PE1899A1 - Metodo para inmortalizar celulas - Google Patents

Metodo para inmortalizar celulas

Info

Publication number
PE1899A1
PE1899A1 PE1997000706A PE00070697A PE1899A1 PE 1899 A1 PE1899 A1 PE 1899A1 PE 1997000706 A PE1997000706 A PE 1997000706A PE 00070697 A PE00070697 A PE 00070697A PE 1899 A1 PE1899 A1 PE 1899A1
Authority
PE
Peru
Prior art keywords
cells
viruses
primary
cell
immortalized cells
Prior art date
Application number
PE1997000706A
Other languages
English (en)
Inventor
Douglas N Foster
Linda K Foster
James A Farris
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of PE1899A1 publication Critical patent/PE1899A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16311—Mardivirus, e.g. Gallid herpesvirus 2, Marek-like viruses, turkey HV
    • C12N2710/16351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Abstract

SE REFIERE A UN METODO PARA TRANSFORMAR CELULAS PRIMARIAS NO PERTENECIENTES A ROEDORES (PREFERENTEMENTE PROCEDENTES DE AVES o HUMANOS), Y, A LAS CELULAS INMORTALIZADAS OBTENIDAS, EN DONDE DICHO METODO COMPRENDE: COLOCAR EL ADN QUE CODIFICA p53 BAJO EL CONTROL DE UN PROMOTOR DE METALOTIONEINA EN UN VECTOR GENICO CAPAZ DE DIRIGIR LA EXPRESION DE p53; INTRODUCIR EL VECTOR GENICO EN DICHAS CELULAS PRIMARIAS; SELECCIONAR FOCOS DE CELULAS (NEGATIVAS A LA TRANSCRIPTASA INVERSA Y NO TUMORIGENICAS) CON PERIODOS DE 0,6 A 1,5 DUPLICACIONES DE POBLACION POR DIA; EN DONDE DICHAS CELULAS PRIMARIAS SE SELECCIONAN DE TEJIDO CUTANEO, DEL MUSCULO DEL PECHO Y/O DEL MUSCULO CARDIACO. TAMBIEN SE REFIERE A METODOS PARA PROPAGAR VIRUS, QUE COMPRENDEN: (A) CONTACTAR AL MENOS UNA PARTICULA VIRAL INFECCIOSA (REOVIRUS, VIRUS DE LA PUSTULACION AVICOLA) CON POR LO MENOS UNA CELULA DE UN CULTIVO DE CELULAS INMORTALIZADAS, Y RECOLECTAR LOS VIRUS PRODUCIDOS; (B) CONTACTAR AL MENOS UNA PARTICULA VIRAL INFECCIOSA CON UNA CELULA PRIMARIA, CULTIVAR LAS CELULAS PRIMARIAS CON CELULAS INMORTALIZADAS, Y RECOLECTAR LOS VIRUS PRODUCIDOS. ESTAS CELULAS INMORTALIZADAS SON USADAS COMO RESERVORIOS LIBRES DE CONTAMINANTES PARA LA PRODUCCION DE PROTEINAS O VIRUS RECOMBINANTES Y COMO SUSTRATOS PARA APOYAR EL RENDIMIENTO DEL VIRUS DURANTE SU PROPAGACION EN OTRAS CELULAS
PE1997000706A 1996-08-13 1997-08-13 Metodo para inmortalizar celulas PE1899A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/696,376 US5830723A (en) 1996-08-13 1996-08-13 Method for immortalizing chicken cells

Publications (1)

Publication Number Publication Date
PE1899A1 true PE1899A1 (es) 1999-02-02

Family

ID=24796804

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1997000706A PE1899A1 (es) 1996-08-13 1997-08-13 Metodo para inmortalizar celulas

Country Status (27)

Country Link
US (1) US5830723A (es)
EP (1) EP0956355B1 (es)
JP (1) JP3833714B2 (es)
KR (1) KR100375872B1 (es)
CN (1) CN1133745C (es)
AR (1) AR008295A1 (es)
AT (1) ATE280236T1 (es)
AU (1) AU717693B2 (es)
BR (1) BR9711165B1 (es)
CA (1) CA2263790C (es)
CO (1) CO4650227A1 (es)
DE (1) DE69731310T2 (es)
DZ (1) DZ2292A1 (es)
ES (1) ES2230619T3 (es)
GT (1) GT199700092A (es)
HK (1) HK1022497A1 (es)
HU (1) HUP9903946A3 (es)
IL (1) IL128512A (es)
MA (1) MA24592A1 (es)
MY (1) MY121698A (es)
NZ (1) NZ334146A (es)
PE (1) PE1899A1 (es)
PT (1) PT956355E (es)
SA (1) SA97180647B1 (es)
UY (1) UY24667A1 (es)
WO (1) WO1998006827A2 (es)
ZA (1) ZA977215B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1161689A1 (en) 1999-03-16 2001-12-12 Serex, Inc. Method and device for detection of apo a, apo b and the ratio thereof in saliva
EP1048725B1 (en) * 1999-04-27 2005-07-06 Transgene S.A. Process for production of mammalian cell lines
EP1048724A1 (en) * 1999-04-27 2000-11-02 Transgene S.A. Human myoblast cell lines and their uses
EP1069187A1 (en) * 1999-07-14 2001-01-17 Stichting Dienst Landbouwkundig Onderzoek Mosaic Infectious Bursal Disease Virus vaccines
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
EP1528101A1 (en) 2003-11-03 2005-05-04 ProBioGen AG Immortalized avian cell lines for virus production
ATE444741T1 (de) 2006-05-10 2009-10-15 Biocompatibles Uk Ltd Glp-1 peptide enthaltende kugelförmige mikrokapseln, deren produktion und deren verwendung
EP2163243A1 (en) 2008-09-12 2010-03-17 Biocompatibles UK Limited Treatment of acute myocardial infarction (AMI) using encapsulated cells encoding and secreting GLP-1 peptides or analogs thereof
EP2251028A1 (en) 2009-05-12 2010-11-17 Biocompatibles Uk Ltd. Treatment of eye diseases using encapsulated cells encoding and secreting an anti-angiogenic factor and/or a neuroprotective factor
US20140004201A1 (en) 2010-09-15 2014-01-02 Biocompatibles Uk Ltd. Treatment of Vascular Diseases Using Encapsulated Cells Encoding and Secreting GLP-1, or a Fragment or Variant Thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2634784A1 (fr) * 1988-08-01 1990-02-02 Inst Nat Sante Rech Med Lignees de cellules epitheliales intestinales immortalisees, leurs procedes de preparation et leurs applications en tant que systeme de production de facteurs de croissance et en tant que modele d'etude de la physiopathologie digestive fonctionnelle et cancereuse
DE4142452A1 (de) * 1991-12-18 1993-06-24 Max Planck Gesellschaft Mittel zur stimulierung der teilungsaktivitaet tierischer zellen
EP0804553A4 (en) * 1993-07-09 1999-07-07 Jolla Cancer Res Found CONDITIONAL IMMORTALIZATION OF GERM CELL LINES
AU2640595A (en) * 1994-05-17 1995-12-05 Rutgers University Immortalized and transformed astrocyte-derived cells

Also Published As

Publication number Publication date
US5830723A (en) 1998-11-03
ZA977215B (en) 1998-02-19
IL128512A0 (en) 2000-01-31
CN1228122A (zh) 1999-09-08
EP0956355A2 (en) 1999-11-17
CA2263790A1 (en) 1998-02-19
UY24667A1 (es) 1998-02-02
CA2263790C (en) 2003-10-14
DE69731310T2 (de) 2006-02-23
CN1133745C (zh) 2004-01-07
MY121698A (en) 2006-02-28
PT956355E (pt) 2005-01-31
HUP9903946A2 (hu) 2000-03-28
AU717693B2 (en) 2000-03-30
BR9711165B1 (pt) 2008-11-18
AR008295A1 (es) 1999-12-29
KR100375872B1 (ko) 2003-03-10
GT199700092A (es) 1997-08-12
JP3833714B2 (ja) 2006-10-18
AU3982997A (en) 1998-03-06
EP0956355B1 (en) 2004-10-20
BR9711165A (pt) 1999-08-17
HK1022497A1 (en) 2000-08-11
ATE280236T1 (de) 2004-11-15
ES2230619T3 (es) 2005-05-01
CO4650227A1 (es) 1998-09-03
DE69731310D1 (de) 2004-11-25
HUP9903946A3 (en) 2000-09-28
JP2000516469A (ja) 2000-12-12
WO1998006827A3 (en) 1998-03-26
NZ334146A (en) 2001-01-26
IL128512A (en) 2005-07-25
SA97180647B1 (ar) 2006-09-05
KR20000029970A (ko) 2000-05-25
WO1998006827A2 (en) 1998-02-19
DZ2292A1 (fr) 2002-12-25
MA24592A1 (fr) 1999-04-01

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Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed