NZ280764A - Expression vector comprising bovine growth hormone gene modified at the 5'end region without changing the encoded protein sequence and a trpa transcription terminator - Google Patents
Expression vector comprising bovine growth hormone gene modified at the 5'end region without changing the encoded protein sequence and a trpa transcription terminatorInfo
- Publication number
- NZ280764A NZ280764A NZ280764A NZ28076495A NZ280764A NZ 280764 A NZ280764 A NZ 280764A NZ 280764 A NZ280764 A NZ 280764A NZ 28076495 A NZ28076495 A NZ 28076495A NZ 280764 A NZ280764 A NZ 280764A
- Authority
- NZ
- New Zealand
- Prior art keywords
- bgh
- expression vector
- growth hormone
- gene
- bovine growth
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 61
- 239000013604 expression vector Substances 0.000 title claims description 30
- 238000013518 transcription Methods 0.000 title claims description 19
- 230000035897 transcription Effects 0.000 title claims description 19
- 108010006025 bovine growth hormone Proteins 0.000 title claims description 12
- 102000004169 proteins and genes Human genes 0.000 title description 13
- 239000013612 plasmid Substances 0.000 claims description 36
- 101150019416 trpA gene Proteins 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 9
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 5
- 230000014621 translational initiation Effects 0.000 claims description 4
- 108091081024 Start codon Proteins 0.000 claims description 3
- 239000012634 fragment Substances 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 22
- 108020004414 DNA Proteins 0.000 description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 239000000872 buffer Substances 0.000 description 14
- 239000000203 mixture Substances 0.000 description 11
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 238000000246 agarose gel electrophoresis Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 108010002350 Interleukin-2 Proteins 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 239000000122 growth hormone Substances 0.000 description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 description 4
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 206010062767 Hypophysitis Diseases 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 235000011148 calcium chloride Nutrition 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 210000003635 pituitary gland Anatomy 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- PLVPPLCLBIEYEA-WAYWQWQTSA-N (z)-3-(1h-indol-3-yl)prop-2-enoic acid Chemical compound C1=CC=C2C(\C=C/C(=O)O)=CNC2=C1 PLVPPLCLBIEYEA-WAYWQWQTSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 108010041952 Calmodulin Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 241001625930 Luria Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- PLVPPLCLBIEYEA-UHFFFAOYSA-N indoleacrylic acid Natural products C1=CC=C2C(C=CC(=O)O)=CNC2=C1 PLVPPLCLBIEYEA-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000003637 steroidlike Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 101150072531 10 gene Proteins 0.000 description 1
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 102000000584 Calmodulin Human genes 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 102000000588 Interleukin-2 Human genes 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- UMSVPCYSAUKCAZ-UHFFFAOYSA-N propane;hydrochloride Chemical compound Cl.CCC UMSVPCYSAUKCAZ-UHFFFAOYSA-N 0.000 description 1
- 229940115271 ralgro Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000000754 repressing effect Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019940040025A KR100225511B1 (ko) | 1994-12-30 | 1994-12-30 | 소 성장호르몬의 대량 생산 방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ280764A true NZ280764A (en) | 1997-02-24 |
Family
ID=36951464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ280764A NZ280764A (en) | 1994-12-30 | 1995-12-21 | Expression vector comprising bovine growth hormone gene modified at the 5'end region without changing the encoded protein sequence and a trpa transcription terminator |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JP3234478B2 (ko) |
| KR (1) | KR100225511B1 (ko) |
| AR (1) | AR000606A1 (ko) |
| AU (1) | AU713218B2 (ko) |
| BG (1) | BG64565B1 (ko) |
| BR (1) | BR9600009A (ko) |
| CO (1) | CO4480064A1 (ko) |
| NZ (1) | NZ280764A (ko) |
| TW (1) | TW505694B (ko) |
| ZA (1) | ZA9511030B (ko) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109182318A (zh) * | 2018-08-16 | 2019-01-11 | 湖北大学 | 一种利用pBV220表达载体高效表达色氨酸合成酶的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0712318B2 (ja) * | 1986-12-31 | 1995-02-15 | 株式会社ラッキー | 合成遺伝子を使用したウシ成長ホルモンの製造方法 |
| DE4101736A1 (de) * | 1991-01-22 | 1992-07-23 | Gruenenthal Gmbh | Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung |
-
1994
- 1994-12-30 KR KR1019940040025A patent/KR100225511B1/ko active IP Right Grant
-
1995
- 1995-12-21 NZ NZ280764A patent/NZ280764A/en not_active IP Right Cessation
- 1995-12-28 JP JP34263295A patent/JP3234478B2/ja not_active Expired - Fee Related
- 1995-12-28 CO CO95062000A patent/CO4480064A1/es unknown
- 1995-12-28 ZA ZA9511030A patent/ZA9511030B/xx unknown
- 1995-12-28 AU AU40768/95A patent/AU713218B2/en not_active Ceased
- 1995-12-28 BG BG100264A patent/BG64565B1/bg unknown
-
1996
- 1996-01-02 AR AR33489496A patent/AR000606A1/es not_active Application Discontinuation
- 1996-01-02 BR BR9600009A patent/BR9600009A/pt not_active IP Right Cessation
- 1996-05-25 TW TW085106241A patent/TW505694B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BG64565B1 (bg) | 2005-07-29 |
| TW505694B (en) | 2002-10-11 |
| JP3234478B2 (ja) | 2001-12-04 |
| BG100264A (bg) | 1996-12-31 |
| AU713218B2 (en) | 1999-11-25 |
| KR960023059A (ko) | 1996-07-18 |
| KR100225511B1 (ko) | 1999-10-15 |
| JPH08228787A (ja) | 1996-09-10 |
| AR000606A1 (es) | 1997-07-10 |
| CO4480064A1 (es) | 1997-07-09 |
| ZA9511030B (en) | 1996-07-09 |
| BR9600009A (pt) | 1998-01-21 |
| AU4076895A (en) | 1996-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2766781B2 (ja) | ウシ成長ホルモンを生産し得る微生物 | |
| US5922583A (en) | Methods for production of recombinant plasmids | |
| US4551433A (en) | Microbial hybrid promoters | |
| CN1131312C (zh) | 在酵母中表达的n末端延伸蛋白 | |
| US4963495A (en) | Secretion of heterologous proteins | |
| EP0067540B1 (en) | Microbial gene promoter/operators | |
| NZ194786A (en) | Nucleotide sequences coding for human proinsulin or human preproinsulin;transformed microorganisms;production of human insulin | |
| US5622840A (en) | Method for controlling gene expression | |
| US5254463A (en) | Method for expression of bovine growth hormone | |
| AU713218B2 (en) | Process for the mass production of bovine growth hormone | |
| US4880910A (en) | Terminal methionyl bovine growth hormone and its use | |
| WO1990001542A1 (en) | Luciferase, luciferase-coding gene, and process for preparing luciferase | |
| EP0134673B1 (en) | Improved plasmid vector and use thereof | |
| US5104796A (en) | High titer production of human somatomedin c | |
| DK156072B (da) | Syntetisk dna-sekvens indeholdende et ribosombindingssted | |
| KR20020061022A (ko) | 항생제 비의존성 항시적 고발현 벡터 및 이를 이용한유전자 발현방법 | |
| US5489529A (en) | DNA for expression of bovine growth hormone | |
| EP0295285B1 (en) | Method for the production of bovine growth hormone using a synthetic gene | |
| US5270180A (en) | Method for the production of salmon growth hormone using a synthetic gene | |
| WO1997041207A2 (en) | Expression of soluble heterologous proteins in bacteria utilizing a thioredoxin/protein expression vector | |
| US5366876A (en) | Method for production of bovine growth hormone using a synthetic gene | |
| DK173822B1 (da) | Fremgangsmåde til fremstilling af bovint væksthormon | |
| CA1302321C (en) | Preparation of polypeptides | |
| EP1008651A2 (en) | Modified DNA sequence coding for hexose oxidase and use hereof | |
| IE914410A1 (en) | A method for enhancing the structural stability of¹recombinant dna expression vectors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| EXPY | Patent expired |