NO822063L - Rekombinant dna-teknikker for fremstilling av relaxin - Google Patents
Rekombinant dna-teknikker for fremstilling av relaxinInfo
- Publication number
- NO822063L NO822063L NO822063A NO822063A NO822063L NO 822063 L NO822063 L NO 822063L NO 822063 A NO822063 A NO 822063A NO 822063 A NO822063 A NO 822063A NO 822063 L NO822063 L NO 822063L
- Authority
- NO
- Norway
- Prior art keywords
- relaxin
- gene
- chain
- plasmid
- synthetic gene
- Prior art date
Links
- 108090000103 Relaxin Proteins 0.000 title claims description 28
- 102000003743 Relaxin Human genes 0.000 title claims description 28
- 238000000034 method Methods 0.000 title claims description 23
- 108020004511 Recombinant DNA Proteins 0.000 title description 2
- 238000002360 preparation method Methods 0.000 title description 2
- 239000013612 plasmid Substances 0.000 claims description 40
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- 108700005078 Synthetic Genes Proteins 0.000 claims description 21
- 238000004519 manufacturing process Methods 0.000 claims description 14
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- 238000003780 insertion Methods 0.000 claims description 4
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- 101800000074 Relaxin A chain Proteins 0.000 claims description 3
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- 101710109558 Relaxin B chain Proteins 0.000 claims description 3
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- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- -1 dimethoxytrityl alcohol Chemical compound 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002608 insulinlike Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 description 1
- 229960001723 oxytocin Drugs 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000003689 pubic bone Anatomy 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010583 slow cooling Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008259 solid foam Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000002254 stillbirth Diseases 0.000 description 1
- 231100000537 stillbirth Toxicity 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/64—Relaxins
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8119138 | 1981-06-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO822063L true NO822063L (no) | 1982-12-23 |
Family
ID=10522709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO822063A NO822063L (no) | 1981-06-22 | 1982-06-21 | Rekombinant dna-teknikker for fremstilling av relaxin |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0068375A3 (fr) |
| JP (1) | JPS5810600A (fr) |
| AU (1) | AU8507282A (fr) |
| DK (1) | DK278682A (fr) |
| NO (1) | NO822063L (fr) |
| PT (1) | PT75093B (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE55479B1 (en) * | 1982-02-12 | 1990-09-26 | Florey Howard Inst | Molecular cloning and characterization of the gene sequence coding for porcine relaxin |
| US5023321A (en) * | 1982-12-13 | 1991-06-11 | Howard Florey Institute Of Experimental Physiology & Medicine | Molecular cloning and characterization of a further gene sequence coding for human relaxin |
| IL70414A (en) * | 1982-12-13 | 1991-06-10 | Florey Howard Inst | Polypeptide having human h2-relaxin activity,double-stranded dna fragments coding therefor,vectors containing the dna fragments and methods for preparing the polypeptide,dna fragments and vectors |
| WO1985000831A1 (fr) * | 1983-08-10 | 1985-02-28 | Amgen | Expression microbienne d'un facteur de croissance semblable a l'insuline |
| US4606699A (en) * | 1984-02-06 | 1986-08-19 | General Electric Company | Compressor casing recess |
| WO1986001515A1 (fr) * | 1984-08-29 | 1986-03-13 | Chiron Corporation | Expression d'activateur de tissue conjonctif humain et peptides associes |
| GB8723662D0 (en) * | 1987-10-08 | 1987-11-11 | British Bio Technology | Synthetic gene |
| AU623518B2 (en) * | 1989-05-04 | 1992-05-14 | Genentech Inc. | Processes and compositions for the isolation of human relaxin |
| US6348327B1 (en) | 1991-12-06 | 2002-02-19 | Genentech, Inc. | Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells |
| ATE241012T1 (de) * | 1993-06-21 | 2003-06-15 | Genentech Inc | Verfahren zur herstellung von humanen relaxin |
| US7259232B1 (en) * | 2000-10-27 | 2007-08-21 | Nymox Pharmaceutical Corporation | Preferred segments of neural thread protein |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| YU257078A (en) * | 1977-11-08 | 1983-12-31 | Genentech Inc | Process for obtaining the code of the structural gene for the microbial transcribing of polypeptides |
-
1982
- 1982-06-19 EP EP82105405A patent/EP0068375A3/fr not_active Withdrawn
- 1982-06-21 JP JP57106645A patent/JPS5810600A/ja active Pending
- 1982-06-21 AU AU85072/82A patent/AU8507282A/en not_active Abandoned
- 1982-06-21 NO NO822063A patent/NO822063L/no unknown
- 1982-06-21 DK DK278682A patent/DK278682A/da not_active Application Discontinuation
- 1982-06-22 PT PT75093A patent/PT75093B/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5810600A (ja) | 1983-01-21 |
| PT75093B (en) | 1983-12-28 |
| EP0068375A2 (fr) | 1983-01-05 |
| EP0068375A3 (fr) | 1983-04-13 |
| AU8507282A (en) | 1983-01-06 |
| PT75093A (en) | 1982-07-01 |
| DK278682A (da) | 1982-12-23 |
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