NO781296L - Reagens for bestemmelse av glukosekonsentrasjoner med glukosedehydrogenase - Google Patents
Reagens for bestemmelse av glukosekonsentrasjoner med glukosedehydrogenaseInfo
- Publication number
- NO781296L NO781296L NO781296A NO781296A NO781296L NO 781296 L NO781296 L NO 781296L NO 781296 A NO781296 A NO 781296A NO 781296 A NO781296 A NO 781296A NO 781296 L NO781296 L NO 781296L
- Authority
- NO
- Norway
- Prior art keywords
- approx
- glucose
- kinetic
- buffer
- reagent
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 52
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims description 50
- 239000008103 glucose Substances 0.000 title claims description 50
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 28
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 20
- 239000000872 buffer Substances 0.000 claims description 19
- 239000005515 coenzyme Substances 0.000 claims description 15
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 14
- 239000008363 phosphate buffer Substances 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 108010020056 Hydrogenase Proteins 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 3
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 claims description 3
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 3
- 235000011009 potassium phosphates Nutrition 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- 239000001488 sodium phosphate Substances 0.000 claims description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 8
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 229950006238 nadide Drugs 0.000 description 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- CXONXVMMINSQBV-NNYOXOHSSA-N (2r,3r,4s,5r)-5-[[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-2-(3-carbamothioylpyridin-1-ium-1-yl)-4-hydroxyoxolan-3-olate Chemical compound NC(=S)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)[O-])=C1 CXONXVMMINSQBV-NNYOXOHSSA-N 0.000 description 1
- BDDLHHRCDSJVKV-UHFFFAOYSA-N 7028-40-2 Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O BDDLHHRCDSJVKV-UHFFFAOYSA-N 0.000 description 1
- CNPRPOBKEGWPEX-UHFFFAOYSA-N 7h-purine;pyridine-3-carboxamide Chemical compound C1=NC=C2NC=NC2=N1.NC(=O)C1=CC=CN=C1 CNPRPOBKEGWPEX-UHFFFAOYSA-N 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000007357 dehydrogenase reaction Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000012209 glucono delta-lactone Nutrition 0.000 description 1
- 229960003681 gluconolactone Drugs 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- -1 thio-NADP Chemical compound 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/791,825 US4120755A (en) | 1977-04-28 | 1977-04-28 | Kinetic method for determination of glucose concentrations with glucose dehydrogenase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO781296L true NO781296L (no) | 1978-10-31 |
Family
ID=25154903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO781296A NO781296L (no) | 1977-04-28 | 1978-04-13 | Reagens for bestemmelse av glukosekonsentrasjoner med glukosedehydrogenase |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US4120755A (nl) |
| JP (1) | JPS5814200B2 (nl) |
| DE (1) | DE2818603A1 (nl) |
| DK (1) | DK186978A (nl) |
| FR (1) | FR2389133A1 (nl) |
| GB (1) | GB1601771A (nl) |
| IT (1) | IT1094968B (nl) |
| NL (1) | NL7803613A (nl) |
| NO (1) | NO781296L (nl) |
| SE (1) | SE7804882L (nl) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1187388A (en) * | 1978-09-20 | 1985-05-21 | American Monitor Corporation | Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays |
| DE3019450A1 (de) * | 1980-05-21 | 1981-11-26 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur gewinnung von glucosedehydrogenase und hierfuer geeigneter mikroorganismus |
| DE3211167A1 (de) * | 1982-03-26 | 1983-09-29 | Merck Patent Gmbh, 6100 Darmstadt | Testsystem und verfahren zur bestimmung von substanzen in fluessigkeiten |
| EP0094161A1 (en) * | 1982-05-07 | 1983-11-16 | Imperial Chemical Industries Plc | Method for determining glucose content of fluid |
| US4394444A (en) * | 1982-06-21 | 1983-07-19 | Miles Laboratories, Inc. | Cofactor indicator compositions |
| US4728608A (en) * | 1985-04-08 | 1988-03-01 | Cambridge Bioscience Corporation | Bacterial screening system with enhanced shelf life |
| GB8600679D0 (en) * | 1986-01-13 | 1986-02-19 | Imp Group Plc | Chemical analysis of tobacco/smoking-related products |
| DE3711881A1 (de) * | 1987-04-08 | 1988-10-27 | Merck Patent Gmbh | Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium |
| US5250415A (en) * | 1987-04-08 | 1993-10-05 | Merck Patent Gesellschaft Mit Beschrankter Haftung | Process for the preparation of glucose dehydrogenase from Bacillus megaterium |
| US5037738A (en) * | 1987-06-03 | 1991-08-06 | Abbott Laboratories | Simultaneous assay for glucose and urea |
| JPH0286779A (ja) * | 1988-09-22 | 1990-03-27 | Amano Pharmaceut Co Ltd | 改良型組換えｄｎａ、それを含む形質転換体及びそれを用いた耐熱性グルコースデヒドロゲナーゼの製造法 |
| SE466158B (sv) | 1989-04-25 | 1992-01-07 | Migrata Uk Ltd | Analysmetod foer glukosbestaemning i helblod |
| SE466157B (sv) * | 1989-04-25 | 1992-01-07 | Migrata Uk Ltd | Saett att bestaemma glukoshalten hos helblod samt engaangskuvett foer detta |
| ATE139801T1 (de) * | 1990-09-18 | 1996-07-15 | Asahi Chemical Ind | Hochsensitives testverfahren für myo-inositol und komposition zur ausführung davon |
| WO1993012254A1 (en) * | 1991-12-12 | 1993-06-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Highly sensitive determination of d-3-hydroxybutyric acid or acetoacetic acid and composition therefor |
| US5487384A (en) * | 1993-02-25 | 1996-01-30 | Blue Marble Research, Inc. | Kinematic assay of plasma glucose concentration without blood sampling |
| US5567581A (en) * | 1995-03-06 | 1996-10-22 | Diagnostic Reagents, Inc. | Method and kit for enzymatically determining the pH of a specimen |
| TW200718785A (en) * | 2005-11-10 | 2007-05-16 | Toyo Boseki | A process for improving the thermal stability of a composition containing a soluble coenzyme conjugated glucose dehydrogenase (GDH) |
| US9347958B2 (en) | 2014-06-27 | 2016-05-24 | Hemocue Ab | Device and method for determination of an analyte in blood |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CS164231B2 (nl) * | 1972-09-28 | 1975-11-07 | ||
| DE2349819A1 (de) * | 1973-10-04 | 1975-04-17 | Eppendorf Geraetebau Netheler | Verfahren zur enzymkinetischen konzentrationsbestimmung eines substrats |
-
1977
- 1977-04-28 US US05/791,825 patent/US4120755A/en not_active Expired - Lifetime
-
1978
- 1978-03-22 GB GB11318/78A patent/GB1601771A/en not_active Expired
- 1978-04-05 NL NL7803613A patent/NL7803613A/nl not_active Application Discontinuation
- 1978-04-13 NO NO781296A patent/NO781296L/no unknown
- 1978-04-27 SE SE7804882A patent/SE7804882L/xx unknown
- 1978-04-27 FR FR7812550A patent/FR2389133A1/fr active Granted
- 1978-04-27 DE DE19782818603 patent/DE2818603A1/de not_active Withdrawn
- 1978-04-28 JP JP53050206A patent/JPS5814200B2/ja not_active Expired
- 1978-04-28 DK DK186978A patent/DK186978A/da not_active Application Discontinuation
- 1978-04-28 IT IT22831/78A patent/IT1094968B/it active
Also Published As
| Publication number | Publication date |
|---|---|
| GB1601771A (en) | 1981-11-04 |
| US4120755A (en) | 1978-10-17 |
| DE2818603A1 (de) | 1978-11-09 |
| DK186978A (da) | 1978-10-29 |
| FR2389133A1 (fr) | 1978-11-24 |
| IT1094968B (it) | 1985-08-10 |
| FR2389133B1 (nl) | 1982-09-10 |
| IT7822831A0 (it) | 1978-04-28 |
| JPS5814200B2 (ja) | 1983-03-17 |
| NL7803613A (nl) | 1978-10-31 |
| SE7804882L (sv) | 1978-10-29 |
| JPS53137199A (en) | 1978-11-30 |
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