NO330689B1

NO330689B1 - Isolert neublastin polynukleotid, ekspresjonsvektor, isolert vertscelle, polypeptid, fremgangsmate for fremstilling av et polypeptid, preparat samt polypeptid for anvendelse som et medikament.

Isolert neublastin polynukleotid, ekspresjonsvektor, isolert vertscelle, polypeptid, fremgangsmate for fremstilling av et polypeptid, preparat samt polypeptid for anvendelse som et medikament. Download PDF

Info

Publication number: NO330689B1
Authority: NO; Norway
Prior art keywords: polypeptide; seq; neublastin; polynucleotide; cells
Prior art date: 1998-07-06

Application number

NO20010088A

Other languages

English (en)

Norwegian (no)

Other versions

NO20010088L (no

NO20010088D0 (no

Inventor

Teit E Johansen

Nikolaj Blom

Claus Hansen

Original Assignee

Nsgene As

Priority date

1998-07-06

Filing date

2001-01-05

Publication date

2011-06-14

1999-07-02 Priority claimed from US09/347,613 external-priority patent/US6593133B1/en

2001-01-05 Application filed by Nsgene As filed Critical Nsgene As

2001-01-05 Publication of NO20010088D0 publication Critical patent/NO20010088D0/no

2001-03-06 Publication of NO20010088L publication Critical patent/NO20010088L/no

2011-06-14 Publication of NO330689B1 publication Critical patent/NO330689B1/no

Links

102100026376 Artemin Human genes 0.000 title claims description 342
101000785776 Homo sapiens Artemin Proteins 0.000 title claims description 277
108090000765 processed proteins & peptides Proteins 0.000 title claims description 187
102000004196 processed proteins & peptides Human genes 0.000 title claims description 168
229920001184 polypeptide Polymers 0.000 title claims description 155
108091033319 polynucleotide Proteins 0.000 title claims description 113
102000040430 polynucleotide Human genes 0.000 title claims description 113
239000002157 polynucleotide Substances 0.000 title claims description 113
239000013604 expression vector Substances 0.000 title claims description 34
238000002360 preparation method Methods 0.000 title claims description 20
238000004519 manufacturing process Methods 0.000 title claims description 15
239000003814 drug Substances 0.000 title claims description 7
229940079593 drug Drugs 0.000 title description 4
-1 isolated host cell Proteins 0.000 title description 2
210000004027 cell Anatomy 0.000 claims description 179
125000003275 alpha amino acid group Chemical group 0.000 claims description 69
238000000034 method Methods 0.000 claims description 60
150000007523 nucleic acids Chemical class 0.000 claims description 60
230000014509 gene expression Effects 0.000 claims description 58
102000039446 nucleic acids Human genes 0.000 claims description 49
108020004707 nucleic acids Proteins 0.000 claims description 49
210000002569 neuron Anatomy 0.000 claims description 41
238000009396 hybridization Methods 0.000 claims description 33
239000002773 nucleotide Substances 0.000 claims description 32
125000003729 nucleotide group Chemical group 0.000 claims description 31
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 30
150000001413 amino acids Chemical class 0.000 claims description 25
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 23
108700026244 Open Reading Frames Proteins 0.000 claims description 15
210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 14
230000000508 neurotrophic effect Effects 0.000 claims description 14
201000010099 disease Diseases 0.000 claims description 13
210000004962 mammalian cell Anatomy 0.000 claims description 12
201000001119 neuropathy Diseases 0.000 claims description 12
230000007823 neuropathy Effects 0.000 claims description 12
230000003902 lesion Effects 0.000 claims description 8
208000033808 peripheral neuropathy Diseases 0.000 claims description 8
206010002026 amyotrophic lateral sclerosis Diseases 0.000 claims description 6
210000003527 eukaryotic cell Anatomy 0.000 claims description 6
230000004770 neurodegeneration Effects 0.000 claims description 6
208000015122 neurodegenerative disease Diseases 0.000 claims description 6
230000000472 traumatic effect Effects 0.000 claims description 6
239000001963 growth medium Substances 0.000 claims description 5
206010012289 Dementia Diseases 0.000 claims description 4
208000018737 Parkinson disease Diseases 0.000 claims description 4
230000002490 cerebral effect Effects 0.000 claims description 4
210000000578 peripheral nerve Anatomy 0.000 claims description 4
230000001225 therapeutic effect Effects 0.000 claims description 4
208000024827 Alzheimer disease Diseases 0.000 claims description 3
208000023105 Huntington disease Diseases 0.000 claims description 3
208000026139 Memory disease Diseases 0.000 claims description 3
208000028389 Nerve injury Diseases 0.000 claims description 3
230000000302 ischemic effect Effects 0.000 claims description 3
230000008764 nerve damage Effects 0.000 claims description 3
210000001178 neural stem cell Anatomy 0.000 claims description 3
239000003937 drug carrier Substances 0.000 claims description 2
230000002538 fungal effect Effects 0.000 claims description 2
210000005253 yeast cell Anatomy 0.000 claims description 2
238000012258 culturing Methods 0.000 claims 1
238000011321 prophylaxis Methods 0.000 claims 1
108090000623 proteins and genes Proteins 0.000 description 91
108020004635 Complementary DNA Proteins 0.000 description 72
238000010804 cDNA synthesis Methods 0.000 description 72
239000002299 complementary DNA Substances 0.000 description 72
108020004414 DNA Proteins 0.000 description 61
102000034615 Glial cell line-derived neurotrophic factor Human genes 0.000 description 57
108091010837 Glial cell line-derived neurotrophic factor Proteins 0.000 description 57
239000012634 fragment Substances 0.000 description 48
235000018102 proteins Nutrition 0.000 description 46
102000004169 proteins and genes Human genes 0.000 description 46
108091028043 Nucleic acid sequence Proteins 0.000 description 34
230000000694 effects Effects 0.000 description 34
239000000523 sample Substances 0.000 description 32
235000001014 amino acid Nutrition 0.000 description 31
239000013612 plasmid Substances 0.000 description 31
210000004556 brain Anatomy 0.000 description 29
210000001519 tissue Anatomy 0.000 description 29
239000002609 medium Substances 0.000 description 27
239000013598 vector Substances 0.000 description 26
229940024606 amino acid Drugs 0.000 description 25
108010025020 Nerve Growth Factor Proteins 0.000 description 23
239000003900 neurotrophic factor Substances 0.000 description 23
VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 22
241000700159 Rattus Species 0.000 description 21
238000002474 experimental method Methods 0.000 description 21
239000000243 solution Substances 0.000 description 21
239000000872 buffer Substances 0.000 description 19
239000003636 conditioned culture medium Substances 0.000 description 19
239000012528 membrane Substances 0.000 description 19
238000012216 screening Methods 0.000 description 19
210000004379 membrane Anatomy 0.000 description 18
238000011282 treatment Methods 0.000 description 17
108091032973 (ribonucleotides)n+m Proteins 0.000 description 16
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
238000006243 chemical reaction Methods 0.000 description 16
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 15
241001465754 Metazoa Species 0.000 description 15
230000000692 anti-sense effect Effects 0.000 description 15
238000002347 injection Methods 0.000 description 15
239000007924 injection Substances 0.000 description 15
102000007072 Nerve Growth Factors Human genes 0.000 description 14
230000001605 fetal effect Effects 0.000 description 14
108091003079 Bovine Serum Albumin Proteins 0.000 description 13
238000000636 Northern blotting Methods 0.000 description 13
DVGHHMFBFOTGLM-UHFFFAOYSA-L fluorogold Chemical compound F[Au][Au]F DVGHHMFBFOTGLM-UHFFFAOYSA-L 0.000 description 13
108020001507 fusion proteins Proteins 0.000 description 13
102000037865 fusion proteins Human genes 0.000 description 13
239000000047 product Substances 0.000 description 13
238000006467 substitution reaction Methods 0.000 description 13
239000003656 tris buffered saline Substances 0.000 description 13
102000002260 Alkaline Phosphatase Human genes 0.000 description 12
108020004774 Alkaline Phosphatase Proteins 0.000 description 12
108091026890 Coding region Proteins 0.000 description 12
238000010367 cloning Methods 0.000 description 12
230000000295 complement effect Effects 0.000 description 12
108020004999 messenger RNA Proteins 0.000 description 12
230000004083 survival effect Effects 0.000 description 12
241000699802 Cricetulus griseus Species 0.000 description 11
108091000117 Tyrosine 3-Monooxygenase Proteins 0.000 description 11
102000048218 Tyrosine 3-monooxygenases Human genes 0.000 description 11
230000003321 amplification Effects 0.000 description 11
229960003638 dopamine Drugs 0.000 description 11
210000001577 neostriatum Anatomy 0.000 description 11
238000003199 nucleic acid amplification method Methods 0.000 description 11
102100023460 Choline O-acetyltransferase Human genes 0.000 description 10
108010058699 Choline O-acetyltransferase Proteins 0.000 description 10
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
208000035475 disorder Diseases 0.000 description 10
210000001259 mesencephalon Anatomy 0.000 description 10
239000012679 serum free medium Substances 0.000 description 10
239000006228 supernatant Substances 0.000 description 10
238000012384 transportation and delivery Methods 0.000 description 10
108010043121 Green Fluorescent Proteins Proteins 0.000 description 9
102000004144 Green Fluorescent Proteins Human genes 0.000 description 9
241001529936 Murinae Species 0.000 description 9
102100021584 Neurturin Human genes 0.000 description 9
239000000499 gel Substances 0.000 description 9
239000005090 green fluorescent protein Substances 0.000 description 9
239000011541 reaction mixture Substances 0.000 description 9
238000001262 western blot Methods 0.000 description 9
108020004705 Codon Proteins 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 8
108010015406 Neurturin Proteins 0.000 description 8
102100036660 Persephin Human genes 0.000 description 8
102000004887 Transforming Growth Factor beta Human genes 0.000 description 8
108090001012 Transforming Growth Factor beta Proteins 0.000 description 8
241000700605 Viruses Species 0.000 description 8
239000011543 agarose gel Substances 0.000 description 8
238000004458 analytical method Methods 0.000 description 8
238000005516 engineering process Methods 0.000 description 8
208000014674 injury Diseases 0.000 description 8
210000004940 nucleus Anatomy 0.000 description 8
DIVDFFZHCJEHGG-UHFFFAOYSA-N oxidopamine Chemical compound NCCC1=CC(O)=C(O)C=C1O DIVDFFZHCJEHGG-UHFFFAOYSA-N 0.000 description 8
108010070453 persephin Proteins 0.000 description 8
239000000825 pharmaceutical preparation Substances 0.000 description 8
210000000278 spinal cord Anatomy 0.000 description 8
ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 8
241000699666 Mus <mouse, genus> Species 0.000 description 7
229920001213 Polysorbate 20 Polymers 0.000 description 7
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 7
210000002257 embryonic structure Anatomy 0.000 description 7
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 7
229960005542 ethidium bromide Drugs 0.000 description 7
239000012091 fetal bovine serum Substances 0.000 description 7
102000050671 human ARTN Human genes 0.000 description 7
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 7
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 7
238000003259 recombinant expression Methods 0.000 description 7
239000011780 sodium chloride Substances 0.000 description 7
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
102000053602 DNA Human genes 0.000 description 6
108700024394 Exon Proteins 0.000 description 6
239000012981 Hank's balanced salt solution Substances 0.000 description 6
101000579425 Homo sapiens Proto-oncogene tyrosine-protein kinase receptor Ret Proteins 0.000 description 6
241000713666 Lentivirus Species 0.000 description 6
241000699670 Mus sp. Species 0.000 description 6
241000283973 Oryctolagus cuniculus Species 0.000 description 6
108010076504 Protein Sorting Signals Proteins 0.000 description 6
102100028286 Proto-oncogene tyrosine-protein kinase receptor Ret Human genes 0.000 description 6
229920002684 Sepharose Polymers 0.000 description 6
238000003556 assay Methods 0.000 description 6
210000003710 cerebral cortex Anatomy 0.000 description 6
230000006378 damage Effects 0.000 description 6
230000003291 dopaminomimetic effect Effects 0.000 description 6
238000001378 electrochemiluminescence detection Methods 0.000 description 6
238000004520 electroporation Methods 0.000 description 6
230000004927 fusion Effects 0.000 description 6
210000005260 human cell Anatomy 0.000 description 6
229940027941 immunoglobulin g Drugs 0.000 description 6
238000001727 in vivo Methods 0.000 description 6
238000002372 labelling Methods 0.000 description 6
239000003446 ligand Substances 0.000 description 6
230000001404 mediated effect Effects 0.000 description 6
210000005036 nerve Anatomy 0.000 description 6
238000012163 sequencing technique Methods 0.000 description 6
241000894007 species Species 0.000 description 6
210000003594 spinal ganglia Anatomy 0.000 description 6
238000010561 standard procedure Methods 0.000 description 6
210000003523 substantia nigra Anatomy 0.000 description 6
238000005382 thermal cycling Methods 0.000 description 6
210000000427 trigeminal ganglion Anatomy 0.000 description 6
241000701161 unidentified adenovirus Species 0.000 description 6
241000283707 Capra Species 0.000 description 5
QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
125000000539 amino acid group Chemical group 0.000 description 5
229940098773 bovine serum albumin Drugs 0.000 description 5
238000010276 construction Methods 0.000 description 5
UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 5
210000005064 dopaminergic neuron Anatomy 0.000 description 5
235000013861 fat-free Nutrition 0.000 description 5
210000001320 hippocampus Anatomy 0.000 description 5
238000011534 incubation Methods 0.000 description 5
238000002955 isolation Methods 0.000 description 5
210000001161 mammalian embryo Anatomy 0.000 description 5
235000013336 milk Nutrition 0.000 description 5
239000008267 milk Substances 0.000 description 5
210000004080 milk Anatomy 0.000 description 5
239000008363 phosphate buffer Substances 0.000 description 5
108020003175 receptors Proteins 0.000 description 5
102000005962 receptors Human genes 0.000 description 5
210000001525 retina Anatomy 0.000 description 5
210000002966 serum Anatomy 0.000 description 5
238000013518 transcription Methods 0.000 description 5
230000035897 transcription Effects 0.000 description 5
238000012546 transfer Methods 0.000 description 5
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 4
238000002965 ELISA Methods 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
241000238631 Hexapoda Species 0.000 description 4
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 4
208000001089 Multiple system atrophy Diseases 0.000 description 4
239000013504 Triton X-100 Substances 0.000 description 4
229920004890 Triton X-100 Polymers 0.000 description 4
108090000631 Trypsin Proteins 0.000 description 4
102000004142 Trypsin Human genes 0.000 description 4
239000002253 acid Substances 0.000 description 4
210000004436 artificial bacterial chromosome Anatomy 0.000 description 4
125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 4
239000002585 base Substances 0.000 description 4
230000004071 biological effect Effects 0.000 description 4
235000011089 carbon dioxide Nutrition 0.000 description 4
210000003169 central nervous system Anatomy 0.000 description 4
239000003795 chemical substances by application Substances 0.000 description 4
238000003776 cleavage reaction Methods 0.000 description 4
150000001875 compounds Chemical class 0.000 description 4
238000001514 detection method Methods 0.000 description 4
239000000975 dye Substances 0.000 description 4
238000001502 gel electrophoresis Methods 0.000 description 4
230000002068 genetic effect Effects 0.000 description 4
210000004408 hybridoma Anatomy 0.000 description 4
238000000338 in vitro Methods 0.000 description 4
238000001990 intravenous administration Methods 0.000 description 4
229930182817 methionine Natural products 0.000 description 4
210000000653 nervous system Anatomy 0.000 description 4
230000001537 neural effect Effects 0.000 description 4
238000012545 processing Methods 0.000 description 4
230000001105 regulatory effect Effects 0.000 description 4
230000007017 scission Effects 0.000 description 4
238000001890 transfection Methods 0.000 description 4
230000008733 trauma Effects 0.000 description 4
239000012588 trypsin Substances 0.000 description 4
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
102000014914 Carrier Proteins Human genes 0.000 description 3
108010078791 Carrier Proteins Proteins 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
241000282412 Homo Species 0.000 description 3
101001136670 Homo sapiens Persephin Proteins 0.000 description 3
108091092195 Intron Proteins 0.000 description 3
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
101100324496 Mus musculus Artn gene Proteins 0.000 description 3
206010028980 Neoplasm Diseases 0.000 description 3
239000000020 Nitrocellulose Substances 0.000 description 3
238000012408 PCR amplification Methods 0.000 description 3
229930040373 Paraformaldehyde Natural products 0.000 description 3
108020004511 Recombinant DNA Proteins 0.000 description 3
229930006000 Sucrose Natural products 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
108700005078 Synthetic Genes Proteins 0.000 description 3
108090000848 Ubiquitin Proteins 0.000 description 3
102000044159 Ubiquitin Human genes 0.000 description 3
241000700618 Vaccinia virus Species 0.000 description 3
208000027418 Wounds and injury Diseases 0.000 description 3
QWXOJIDBSHLIFI-UHFFFAOYSA-N [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate Chemical compound O1OC2(C3CC4CC2CC(Cl)(C4)C3)C1(OC)C1=CC=CC(OP(O)(O)=O)=C1 QWXOJIDBSHLIFI-UHFFFAOYSA-N 0.000 description 3
230000002159 abnormal effect Effects 0.000 description 3
150000007513 acids Chemical class 0.000 description 3
230000004913 activation Effects 0.000 description 3
239000000427 antigen Substances 0.000 description 3
108091007433 antigens Proteins 0.000 description 3
102000036639 antigens Human genes 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
210000004369 blood Anatomy 0.000 description 3
239000008280 blood Substances 0.000 description 3
210000005013 brain tissue Anatomy 0.000 description 3
210000004899 c-terminal region Anatomy 0.000 description 3
201000011510 cancer Diseases 0.000 description 3
210000001638 cerebellum Anatomy 0.000 description 3
210000001175 cerebrospinal fluid Anatomy 0.000 description 3
238000004590 computer program Methods 0.000 description 3
230000007547 defect Effects 0.000 description 3
238000004925 denaturation Methods 0.000 description 3
230000036425 denaturation Effects 0.000 description 3
238000003745 diagnosis Methods 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
239000003623 enhancer Substances 0.000 description 3
230000006870 function Effects 0.000 description 3
210000002216 heart Anatomy 0.000 description 3
238000004128 high performance liquid chromatography Methods 0.000 description 3
235000014304 histidine Nutrition 0.000 description 3
239000007943 implant Substances 0.000 description 3
238000007901 in situ hybridization Methods 0.000 description 3
230000001939 inductive effect Effects 0.000 description 3
208000015181 infectious disease Diseases 0.000 description 3
230000000977 initiatory effect Effects 0.000 description 3
230000003993 interaction Effects 0.000 description 3
210000003734 kidney Anatomy 0.000 description 3
210000004185 liver Anatomy 0.000 description 3
210000004072 lung Anatomy 0.000 description 3
238000012986 modification Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
239000013642 negative control Substances 0.000 description 3
229920001220 nitrocellulos Polymers 0.000 description 3
210000000287 oocyte Anatomy 0.000 description 3
210000001672 ovary Anatomy 0.000 description 3
210000000496 pancreas Anatomy 0.000 description 3
229920002866 paraformaldehyde Polymers 0.000 description 3
239000002245 particle Substances 0.000 description 3
102000054765 polymorphisms of proteins Human genes 0.000 description 3
230000001323 posttranslational effect Effects 0.000 description 3
239000002243 precursor Substances 0.000 description 3
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
230000008569 process Effects 0.000 description 3
210000004129 prosencephalon Anatomy 0.000 description 3
238000003757 reverse transcription PCR Methods 0.000 description 3
210000001202 rhombencephalon Anatomy 0.000 description 3
210000002027 skeletal muscle Anatomy 0.000 description 3
239000011734 sodium Substances 0.000 description 3
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
239000000758 substrate Substances 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
239000000725 suspension Substances 0.000 description 3
210000001103 thalamus Anatomy 0.000 description 3
238000013519 translation Methods 0.000 description 3
230000001228 trophic effect Effects 0.000 description 3
241001430294 unidentified retrovirus Species 0.000 description 3
238000005406 washing Methods 0.000 description 3
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 2
108020003589 5' Untranslated Regions Proteins 0.000 description 2
206010002091 Anaesthesia Diseases 0.000 description 2
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
OBMZMSLWNNWEJA-XNCRXQDQSA-N C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 Chemical compound C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 OBMZMSLWNNWEJA-XNCRXQDQSA-N 0.000 description 2
108091006146 Channels Proteins 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
241001573498 Compacta Species 0.000 description 2
241000699800 Cricetinae Species 0.000 description 2
239000003298 DNA probe Substances 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
102000016911 Deoxyribonucleases Human genes 0.000 description 2
108010053770 Deoxyribonucleases Proteins 0.000 description 2
229920002307 Dextran Polymers 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
101001038376 Homo sapiens GDNF family receptor alpha-3 Proteins 0.000 description 2
108010001336 Horseradish Peroxidase Proteins 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
239000012580 N-2 Supplement Substances 0.000 description 2
208000012902 Nervous system disease Diseases 0.000 description 2
CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
239000002033 PVDF binder Substances 0.000 description 2
208000037273 Pathologic Processes Diseases 0.000 description 2
101710176384 Peptide 1 Proteins 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
241000276498 Pollachius virens Species 0.000 description 2
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
108010029485 Protein Isoforms Proteins 0.000 description 2
102000001708 Protein Isoforms Human genes 0.000 description 2
241000283984 Rodentia Species 0.000 description 2
108091081024 Start codon Proteins 0.000 description 2
208000006011 Stroke Diseases 0.000 description 2
101150057615 Syn gene Proteins 0.000 description 2
108010006785 Taq Polymerase Proteins 0.000 description 2
241001492404 Woodchuck hepatitis virus Species 0.000 description 2
230000037005 anaesthesia Effects 0.000 description 2
238000000137 annealing Methods 0.000 description 2
230000002788 anti-peptide Effects 0.000 description 2
210000003719 b-lymphocyte Anatomy 0.000 description 2
239000011324 bead Substances 0.000 description 2
230000000975 bioactive effect Effects 0.000 description 2
208000029028 brain injury Diseases 0.000 description 2
238000009395 breeding Methods 0.000 description 2
230000001488 breeding effect Effects 0.000 description 2
239000001506 calcium phosphate Substances 0.000 description 2
229910000389 calcium phosphate Inorganic materials 0.000 description 2
235000011010 calcium phosphates Nutrition 0.000 description 2
238000004364 calculation method Methods 0.000 description 2
210000001159 caudate nucleus Anatomy 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
230000001713 cholinergic effect Effects 0.000 description 2
238000003501 co-culture Methods 0.000 description 2
238000007796 conventional method Methods 0.000 description 2
238000001816 cooling Methods 0.000 description 2
230000001351 cycling effect Effects 0.000 description 2
230000007850 degeneration Effects 0.000 description 2
238000002716 delivery method Methods 0.000 description 2
230000004069 differentiation Effects 0.000 description 2
230000029087 digestion Effects 0.000 description 2
239000012153 distilled water Substances 0.000 description 2
231100000673 dose–response relationship Toxicity 0.000 description 2
238000001962 electrophoresis Methods 0.000 description 2
210000002472 endoplasmic reticulum Anatomy 0.000 description 2
210000002889 endothelial cell Anatomy 0.000 description 2
229940088598 enzyme Drugs 0.000 description 2
238000000605 extraction Methods 0.000 description 2
210000002950 fibroblast Anatomy 0.000 description 2
210000000609 ganglia Anatomy 0.000 description 2
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
108091005608 glycosylated proteins Proteins 0.000 description 2
102000035122 glycosylated proteins Human genes 0.000 description 2
210000003128 head Anatomy 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
238000003364 immunohistochemistry Methods 0.000 description 2
238000012744 immunostaining Methods 0.000 description 2
238000007918 intramuscular administration Methods 0.000 description 2
238000007912 intraperitoneal administration Methods 0.000 description 2
238000007913 intrathecal administration Methods 0.000 description 2
238000007914 intraventricular administration Methods 0.000 description 2
PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
238000001638 lipofection Methods 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
210000005171 mammalian brain Anatomy 0.000 description 2
238000013507 mapping Methods 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
238000005259 measurement Methods 0.000 description 2
208000030159 metabolic disease Diseases 0.000 description 2
238000000520 microinjection Methods 0.000 description 2
239000000203 mixture Substances 0.000 description 2
210000002161 motor neuron Anatomy 0.000 description 2
238000002887 multiple sequence alignment Methods 0.000 description 2
230000032405 negative regulation of neuron apoptotic process Effects 0.000 description 2
230000007830 nerve conduction Effects 0.000 description 2
210000005155 neural progenitor cell Anatomy 0.000 description 2
210000000956 olfactory bulb Anatomy 0.000 description 2
229940046166 oligodeoxynucleotide Drugs 0.000 description 2
208000031237 olivopontocerebellar atrophy Diseases 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
230000009054 pathological process Effects 0.000 description 2
239000008188 pellet Substances 0.000 description 2
WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 2
208000027232 peripheral nervous system disease Diseases 0.000 description 2
230000035790 physiological processes and functions Effects 0.000 description 2
229920002401 polyacrylamide Polymers 0.000 description 2
238000003752 polymerase chain reaction Methods 0.000 description 2
229920000136 polysorbate Polymers 0.000 description 2
229920002981 polyvinylidene fluoride Polymers 0.000 description 2
230000004481 post-translational protein modification Effects 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
201000002212 progressive supranuclear palsy Diseases 0.000 description 2
230000009465 prokaryotic expression Effects 0.000 description 2
KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 2
238000011002 quantification Methods 0.000 description 2
238000011160 research Methods 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
238000007894 restriction fragment length polymorphism technique Methods 0.000 description 2
230000000717 retained effect Effects 0.000 description 2
239000012723 sample buffer Substances 0.000 description 2
210000000813 small intestine Anatomy 0.000 description 2
238000013223 sprague-dawley female rat Methods 0.000 description 2
238000010186 staining Methods 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
238000007920 subcutaneous administration Methods 0.000 description 2
238000001356 surgical procedure Methods 0.000 description 2
238000012360 testing method Methods 0.000 description 2
230000000542 thalamic effect Effects 0.000 description 2
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
241000701447 unidentified baculovirus Species 0.000 description 2
241001529453 unidentified herpesvirus Species 0.000 description 2
239000013603 viral vector Substances 0.000 description 2
230000003612 virological effect Effects 0.000 description 2
239000008096 xylene Substances 0.000 description 2
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 1
108020004463 18S ribosomal RNA Chemical group 0.000 description 1
SNBCLPGEMZEWLU-QXFUBDJGSA-N 2-chloro-n-[[(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methyl]acetamide Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CNC(=O)CCl)[C@@H](O)C1 SNBCLPGEMZEWLU-QXFUBDJGSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
108020005345 3' Untranslated Regions Proteins 0.000 description 1
HJFCVJKLGPYQDB-UHFFFAOYSA-N 5-(4-aminophenyl)cyclohexa-2,4-diene-1,1,2-triamine Chemical compound C1C(N)(N)C(N)=CC=C1C1=CC=C(N)C=C1 HJFCVJKLGPYQDB-UHFFFAOYSA-N 0.000 description 1
QRXMUCSWCMTJGU-UHFFFAOYSA-N 5-bromo-4-chloro-3-indolyl phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-N 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
108700028369 Alleles Proteins 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
241000972773 Aulopiformes Species 0.000 description 1
208000000412 Avitaminosis Diseases 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
206010006187 Breast cancer Diseases 0.000 description 1
208000026310 Breast neoplasm Diseases 0.000 description 1
241000208199 Buxus sempervirens Species 0.000 description 1
101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
241000178270 Canarypox virus Species 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
201000009030 Carcinoma Diseases 0.000 description 1
108010062745 Chloride Channels Proteins 0.000 description 1
102000011045 Chloride Channels Human genes 0.000 description 1
102000029816 Collagenase Human genes 0.000 description 1
108060005980 Collagenase Proteins 0.000 description 1
108020004394 Complementary RNA Proteins 0.000 description 1
206010010356 Congenital anomaly Diseases 0.000 description 1
101150074155 DHFR gene Proteins 0.000 description 1
108020003215 DNA Probes Proteins 0.000 description 1
230000005778 DNA damage Effects 0.000 description 1
231100000277 DNA damage Toxicity 0.000 description 1
238000000018 DNA microarray Methods 0.000 description 1
230000006820 DNA synthesis Effects 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
241000702421 Dependoparvovirus Species 0.000 description 1
208000032131 Diabetic Neuropathies Diseases 0.000 description 1
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
101100187684 Drosophila melanogaster Ntmt gene Proteins 0.000 description 1
241001269524 Dura Species 0.000 description 1
102100031780 Endonuclease Human genes 0.000 description 1
108010067770 Endopeptidase K Proteins 0.000 description 1
108010013369 Enteropeptidase Proteins 0.000 description 1
102100029727 Enteropeptidase Human genes 0.000 description 1
241000991587 Enterovirus C Species 0.000 description 1
241001302160 Escherichia coli str. K-12 substr. DH10B Species 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
239000004606 Fillers/Extenders Substances 0.000 description 1
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
241000233866 Fungi Species 0.000 description 1
208000003098 Ganglion Cysts Diseases 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
208000010412 Glaucoma Diseases 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
101100175482 Glycine max CG-3 gene Proteins 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
229920000209 Hexadimethrine bromide Polymers 0.000 description 1
108090000144 Human Proteins Proteins 0.000 description 1
102000003839 Human Proteins Human genes 0.000 description 1
241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
101100321817 Human parvovirus B19 (strain HV) 7.5K gene Proteins 0.000 description 1
206010021135 Hypovitaminosis Diseases 0.000 description 1
XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
208000032382 Ischaemic stroke Diseases 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
XUIIKFGFIJCVMT-LBPRGKRZSA-N L-thyroxine Chemical compound IC1=CC(C[C@H]([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-LBPRGKRZSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
239000012741 Laemmli sample buffer Substances 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
HLFSDGLLUJUHTE-SNVBAGLBSA-N Levamisole Chemical compound C1([C@H]2CN3CCSC3=N2)=CC=CC=C1 HLFSDGLLUJUHTE-SNVBAGLBSA-N 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
241000712079 Measles morbillivirus Species 0.000 description 1
102000003792 Metallothionein Human genes 0.000 description 1
108090000157 Metallothionein Proteins 0.000 description 1
241001460678 Napo <wasp> Species 0.000 description 1
208000025966 Neurological disease Diseases 0.000 description 1
102000019040 Nuclear Antigens Human genes 0.000 description 1
108010051791 Nuclear Antigens Proteins 0.000 description 1
108020004711 Nucleic Acid Probes Proteins 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
108020005187 Oligonucleotide Probes Proteins 0.000 description 1
108700020796 Oncogene Proteins 0.000 description 1
206010033128 Ovarian cancer Diseases 0.000 description 1
229930012538 Paclitaxel Natural products 0.000 description 1
108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
208000027089 Parkinsonian disease Diseases 0.000 description 1
206010034010 Parkinsonism Diseases 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
108010033276 Peptide Fragments Proteins 0.000 description 1
102000007079 Peptide Fragments Human genes 0.000 description 1
208000010886 Peripheral nerve injury Diseases 0.000 description 1
108010002747 Pfu DNA polymerase Proteins 0.000 description 1
206010035226 Plasma cell myeloma Diseases 0.000 description 1
206010036105 Polyneuropathy Diseases 0.000 description 1
239000005700 Putrescine Substances 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
241000700157 Rattus norvegicus Species 0.000 description 1
101000997959 Rattus norvegicus GDNF family receptor alpha-1 Proteins 0.000 description 1
101100068253 Rattus norvegicus Gdnf gene Proteins 0.000 description 1
101100248018 Rattus norvegicus Ret gene Proteins 0.000 description 1
208000007014 Retinitis pigmentosa Diseases 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
241000702208 Shigella phage SfX Species 0.000 description 1
208000009106 Shy-Drager Syndrome Diseases 0.000 description 1
241000710960 Sindbis virus Species 0.000 description 1
108020004682 Single-Stranded DNA Proteins 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
208000005400 Synovial Cyst Diseases 0.000 description 1
210000001744 T-lymphocyte Anatomy 0.000 description 1
108010034949 Thyroglobulin Proteins 0.000 description 1
102000009843 Thyroglobulin Human genes 0.000 description 1
AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
108700019146 Transgenes Proteins 0.000 description 1
239000007984 Tris EDTA buffer Substances 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
108091023045 Untranslated Region Proteins 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
241000269368 Xenopus laevis Species 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
230000001594 aberrant effect Effects 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
239000008186 active pharmaceutical agent Substances 0.000 description 1
230000001154 acute effect Effects 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
239000000443 aerosol Substances 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
238000012197 amplification kit Methods 0.000 description 1
210000004727 amygdala Anatomy 0.000 description 1
238000010171 animal model Methods 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
230000002927 anti-mitotic effect Effects 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000003080 antimitotic agent Substances 0.000 description 1
239000002246 antineoplastic agent Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
150000001483 arginine derivatives Chemical class 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
210000001130 astrocyte Anatomy 0.000 description 1
230000002238 attenuated effect Effects 0.000 description 1
230000002567 autonomic effect Effects 0.000 description 1
230000003376 axonal effect Effects 0.000 description 1
108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 description 1
108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
239000003855 balanced salt solution Substances 0.000 description 1
230000006399 behavior Effects 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
239000001045 blue dye Substances 0.000 description 1
230000037396 body weight Effects 0.000 description 1
238000009835 boiling Methods 0.000 description 1
210000002798 bone marrow cell Anatomy 0.000 description 1
239000012888 bovine serum Substances 0.000 description 1
210000000069 breast epithelial cell Anatomy 0.000 description 1
238000000339 bright-field microscopy Methods 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
239000007975 buffered saline Substances 0.000 description 1
239000008366 buffered solution Substances 0.000 description 1
238000004422 calculation algorithm Methods 0.000 description 1
239000002775 capsule Substances 0.000 description 1
239000000969 carrier Substances 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
210000003850 cellular structure Anatomy 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
238000002512 chemotherapy Methods 0.000 description 1
RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 1
229960002327 chloral hydrate Drugs 0.000 description 1
210000002932 cholinergic neuron Anatomy 0.000 description 1
DQLATGHUWYMOKM-UHFFFAOYSA-L cisplatin Chemical compound N[Pt](N)(Cl)Cl DQLATGHUWYMOKM-UHFFFAOYSA-L 0.000 description 1
229960004316 cisplatin Drugs 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
229960002424 collagenase Drugs 0.000 description 1
239000003184 complementary RNA Substances 0.000 description 1
239000002131 composite material Substances 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
239000012468 concentrated sample Substances 0.000 description 1
239000013068 control sample Substances 0.000 description 1
210000000877 corpus callosum Anatomy 0.000 description 1
229960002126 creosote Drugs 0.000 description 1
238000012136 culture method Methods 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
230000005786 degenerative changes Effects 0.000 description 1
230000022811 deglycosylation Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
230000003210 demyelinating effect Effects 0.000 description 1
210000001947 dentate gyrus Anatomy 0.000 description 1
210000004513 dentition Anatomy 0.000 description 1
239000005549 deoxyribonucleoside Substances 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
230000008021 deposition Effects 0.000 description 1
238000013461 design Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
239000008121 dextrose Substances 0.000 description 1
206010012601 diabetes mellitus Diseases 0.000 description 1
238000010586 diagram Methods 0.000 description 1
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
239000000539 dimer Substances 0.000 description 1
208000037765 diseases and disorders Diseases 0.000 description 1
BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
108010007093 dispase Proteins 0.000 description 1
238000002224 dissection Methods 0.000 description 1
238000009826 distribution Methods 0.000 description 1
239000002552 dosage form Substances 0.000 description 1
238000003134 dye exclusion method Methods 0.000 description 1
238000000835 electrochemical detection Methods 0.000 description 1
230000013020 embryo development Effects 0.000 description 1
210000002919 epithelial cell Anatomy 0.000 description 1
239000005038 ethylene vinyl acetate Substances 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
210000001508 eye Anatomy 0.000 description 1
239000012894 fetal calf serum Substances 0.000 description 1
229940126864 fibroblast growth factor Drugs 0.000 description 1
239000000834 fixative Substances 0.000 description 1
238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
239000008098 formaldehyde solution Substances 0.000 description 1
238000009472 formulation Methods 0.000 description 1
239000012458 free base Substances 0.000 description 1
238000007710 freezing Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
238000001476 gene delivery Methods 0.000 description 1
238000010363 gene targeting Methods 0.000 description 1
230000009395 genetic defect Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
244000144993 groups of animals Species 0.000 description 1
230000012010 growth Effects 0.000 description 1
210000003780 hair follicle Anatomy 0.000 description 1
230000035876 healing Effects 0.000 description 1
210000005003 heart tissue Anatomy 0.000 description 1
210000003958 hematopoietic stem cell Anatomy 0.000 description 1
108060003552 hemocyanin Proteins 0.000 description 1
210000003494 hepatocyte Anatomy 0.000 description 1
150000002411 histidines Chemical class 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
125000001165 hydrophobic group Chemical group 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
230000003053 immunization Effects 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
230000016784 immunoglobulin production Effects 0.000 description 1
238000002513 implantation Methods 0.000 description 1
210000004283 incisor Anatomy 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
239000012678 infectious agent Substances 0.000 description 1
238000013383 initial experiment Methods 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
210000002490 intestinal epithelial cell Anatomy 0.000 description 1
210000000936 intestine Anatomy 0.000 description 1
238000001361 intraarterial administration Methods 0.000 description 1
238000007917 intracranial administration Methods 0.000 description 1
238000010253 intravenous injection Methods 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
208000028867 ischemia Diseases 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
238000005304 joining Methods 0.000 description 1
210000002510 keratinocyte Anatomy 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
208000032839 leukemia Diseases 0.000 description 1
229960001614 levamisole Drugs 0.000 description 1
229950008325 levothyroxine Drugs 0.000 description 1
239000002502 liposome Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000012160 loading buffer Substances 0.000 description 1
230000004807 localization Effects 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
208000002780 macular degeneration Diseases 0.000 description 1
FKHVEXUWEQFKCJ-UHFFFAOYSA-L magnesium;sodium;5-ethyl-5-pentan-2-yl-1,3-diazinane-2,4,6-trione;2,2,2-trichloroethane-1,1-diol;sulfate Chemical compound [Na+].[Mg+2].[O-]S([O-])(=O)=O.OC(O)C(Cl)(Cl)Cl.CCCC(C)C1(CC)C(=O)NC(=O)NC1=O FKHVEXUWEQFKCJ-UHFFFAOYSA-L 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000036210 malignancy Effects 0.000 description 1
239000003550 marker Substances 0.000 description 1
201000001441 melanoma Diseases 0.000 description 1
210000003975 mesenteric artery Anatomy 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000007491 morphometric analysis Methods 0.000 description 1
208000005264 motor neuron disease Diseases 0.000 description 1
201000006417 multiple sclerosis Diseases 0.000 description 1
210000000663 muscle cell Anatomy 0.000 description 1
230000035772 mutation Effects 0.000 description 1
201000000050 myeloid neoplasm Diseases 0.000 description 1
239000006199 nebulizer Substances 0.000 description 1
210000000944 nerve tissue Anatomy 0.000 description 1
206010061311 nervous system neoplasm Diseases 0.000 description 1
210000004498 neuroglial cell Anatomy 0.000 description 1
230000003959 neuroinflammation Effects 0.000 description 1
230000006576 neuronal survival Effects 0.000 description 1
239000002581 neurotoxin Substances 0.000 description 1
231100000618 neurotoxin Toxicity 0.000 description 1
230000003472 neutralizing effect Effects 0.000 description 1
JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
210000000584 nodose ganglion Anatomy 0.000 description 1
239000002853 nucleic acid probe Substances 0.000 description 1
235000018343 nutrient deficiency Nutrition 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
239000002751 oligonucleotide probe Substances 0.000 description 1
238000005457 optimization Methods 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
229960001592 paclitaxel Drugs 0.000 description 1
230000036961 partial effect Effects 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
229960001412 pentobarbital Drugs 0.000 description 1
230000000737 periodic effect Effects 0.000 description 1
102000013415 peroxidase activity proteins Human genes 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
239000008194 pharmaceutical composition Substances 0.000 description 1
239000000546 pharmaceutical excipient Substances 0.000 description 1
238000001050 pharmacotherapy Methods 0.000 description 1
108091005981 phosphorylated proteins Proteins 0.000 description 1
108091008695 photoreceptors Proteins 0.000 description 1
238000011197 physicochemical method Methods 0.000 description 1
239000006187 pill Substances 0.000 description 1
230000001817 pituitary effect Effects 0.000 description 1
210000002826 placenta Anatomy 0.000 description 1
239000013600 plasmid vector Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
230000007824 polyneuropathy Effects 0.000 description 1
108010055896 polyornithine Proteins 0.000 description 1
229920002714 polyornithine Polymers 0.000 description 1
108091005626 post-translationally modified proteins Proteins 0.000 description 1
102000035123 post-translationally modified proteins Human genes 0.000 description 1
230000001124 posttranscriptional effect Effects 0.000 description 1
238000012257 pre-denaturation Methods 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
230000001566 pro-viral effect Effects 0.000 description 1
239000000186 progesterone Substances 0.000 description 1
229960003387 progesterone Drugs 0.000 description 1
238000004393 prognosis Methods 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
210000002307 prostate Anatomy 0.000 description 1
238000000746 purification Methods 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
238000003127 radioimmunoassay Methods 0.000 description 1
238000011552 rat model Methods 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000008085 renal dysfunction Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
229920005989 resin Polymers 0.000 description 1
239000011347 resin Substances 0.000 description 1
210000003994 retinal ganglion cell Anatomy 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
229910052594 sapphire Inorganic materials 0.000 description 1
239000010980 sapphire Substances 0.000 description 1
229940016590 sarkosyl Drugs 0.000 description 1
108700004121 sarkosyl Proteins 0.000 description 1
230000003248 secreting effect Effects 0.000 description 1
230000001953 sensory effect Effects 0.000 description 1
210000000413 sensory ganglia Anatomy 0.000 description 1
210000001044 sensory neuron Anatomy 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
230000011664 signaling Effects 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
238000001542 size-exclusion chromatography Methods 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
239000011781 sodium selenite Substances 0.000 description 1
229960001471 sodium selenite Drugs 0.000 description 1
235000015921 sodium selenite Nutrition 0.000 description 1
208000020431 spinal cord injury Diseases 0.000 description 1
208000002320 spinal muscular atrophy Diseases 0.000 description 1
210000000273 spinal nerve root Anatomy 0.000 description 1
210000000952 spleen Anatomy 0.000 description 1
230000010473 stable expression Effects 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
238000010254 subcutaneous injection Methods 0.000 description 1
239000007929 subcutaneous injection Substances 0.000 description 1
210000004281 subthalamic nucleus Anatomy 0.000 description 1
FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 1
239000013589 supplement Substances 0.000 description 1
208000011580 syndromic disease Diseases 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000012385 systemic delivery Methods 0.000 description 1
239000003826 tablet Substances 0.000 description 1
RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 description 1
229960000814 tetanus toxoid Drugs 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
229960002175 thyroglobulin Drugs 0.000 description 1
230000036346 tooth eruption Effects 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
231100000167 toxic agent Toxicity 0.000 description 1
239000003440 toxic substance Substances 0.000 description 1
239000003053 toxin Substances 0.000 description 1
231100000765 toxin Toxicity 0.000 description 1
108700012359 toxins Proteins 0.000 description 1
210000003437 trachea Anatomy 0.000 description 1
230000005030 transcription termination Effects 0.000 description 1
230000009466 transformation Effects 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
230000010474 transient expression Effects 0.000 description 1
238000002054 transplantation Methods 0.000 description 1
230000008736 traumatic injury Effects 0.000 description 1
229940035722 triiodothyronine Drugs 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
229910052721 tungsten Inorganic materials 0.000 description 1
239000010937 tungsten Substances 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
239000004474 valine Substances 0.000 description 1
210000000752 vestibulocochlear nerve Anatomy 0.000 description 1
210000002845 virion Anatomy 0.000 description 1
210000000239 visual pathway Anatomy 0.000 description 1
230000004400 visual pathway Effects 0.000 description 1
208000030401 vitamin deficiency disease Diseases 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1