NO329791B1 - Transposon-mediert multipleks sekvensering - Google Patents
Transposon-mediert multipleks sekvensering Download PDFInfo
- Publication number
- NO329791B1 NO329791B1 NO20026206A NO20026206A NO329791B1 NO 329791 B1 NO329791 B1 NO 329791B1 NO 20026206 A NO20026206 A NO 20026206A NO 20026206 A NO20026206 A NO 20026206A NO 329791 B1 NO329791 B1 NO 329791B1
- Authority
- NO
- Norway
- Prior art keywords
- transposon
- vector
- target dna
- pcr
- dna
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title claims description 41
- 230000001404 mediated effect Effects 0.000 title claims description 7
- 239000013598 vector Substances 0.000 claims description 114
- 108020004414 DNA Proteins 0.000 claims description 88
- 238000000034 method Methods 0.000 claims description 55
- 238000006243 chemical reaction Methods 0.000 claims description 51
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 42
- 102000053602 DNA Human genes 0.000 claims description 25
- 238000003780 insertion Methods 0.000 claims description 20
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- 230000037431 insertion Effects 0.000 claims description 19
- 238000003752 polymerase chain reaction Methods 0.000 claims description 11
- 230000000295 complement effect Effects 0.000 claims description 7
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- 239000007850 fluorescent dye Substances 0.000 claims description 5
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 claims description 2
- 238000011176 pooling Methods 0.000 claims description 2
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 6
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- 239000000872 buffer Substances 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
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- 238000003199 nucleic acid amplification method Methods 0.000 description 4
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- 102100031629 COP9 signalosome complex subunit 1 Human genes 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
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- XZNUGFQTQHRASN-XQENGBIVSA-N apramycin Chemical compound O([C@H]1O[C@@H]2[C@H](O)[C@@H]([C@H](O[C@H]2C[C@H]1N)O[C@@H]1[C@@H]([C@@H](O)[C@H](N)[C@@H](CO)O1)O)NC)[C@@H]1[C@@H](N)C[C@@H](N)[C@H](O)[C@H]1O XZNUGFQTQHRASN-XQENGBIVSA-N 0.000 description 3
- 229950006334 apramycin Drugs 0.000 description 3
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- 238000006713 insertion reaction Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
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- 101000940485 Homo sapiens COP9 signalosome complex subunit 1 Proteins 0.000 description 2
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- 238000002955 isolation Methods 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108050003510 COP9 signalosome complex subunit 1 Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 108091060211 Expressed sequence tag Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
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- 239000012807 PCR reagent Substances 0.000 description 1
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- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 101150050575 URA3 gene Proteins 0.000 description 1
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- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
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- 102000013165 exonuclease Human genes 0.000 description 1
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- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
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- 108020004999 messenger RNA Proteins 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
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- 241000894007 species Species 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21638100P | 2000-07-07 | 2000-07-07 | |
| PCT/US2001/021269 WO2002004674A2 (en) | 2000-07-07 | 2001-07-05 | Transposon mediated multiplex sequencing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO20026206D0 NO20026206D0 (no) | 2002-12-23 |
| NO20026206L NO20026206L (no) | 2003-02-25 |
| NO329791B1 true NO329791B1 (no) | 2010-12-20 |
Family
ID=22806831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20026206A NO329791B1 (no) | 2000-07-07 | 2002-12-23 | Transposon-mediert multipleks sekvensering |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US7229798B2 (ko) |
| EP (1) | EP1327000B1 (ko) |
| JP (1) | JP4807921B2 (ko) |
| KR (1) | KR100823857B1 (ko) |
| AR (1) | AR029582A1 (ko) |
| AT (1) | ATE361994T1 (ko) |
| AU (2) | AU7318501A (ko) |
| BR (1) | BR0112261B1 (ko) |
| CA (1) | CA2415786C (ko) |
| DE (1) | DE60128379T2 (ko) |
| IL (2) | IL153797A0 (ko) |
| MX (1) | MXPA03000038A (ko) |
| NO (1) | NO329791B1 (ko) |
| NZ (1) | NZ523507A (ko) |
| TW (1) | TWI235180B (ko) |
| WO (1) | WO2002004674A2 (ko) |
| ZA (1) | ZA200300035B (ko) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2425011A1 (en) * | 2009-04-29 | 2012-03-07 | Hendrix Genetics Research, Technology & Services B.V. | Method of pooling samples for performing a biological assay |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6174670B1 (en) * | 1996-06-04 | 2001-01-16 | University Of Utah Research Foundation | Monitoring amplification of DNA during PCR |
| ATE374821T1 (de) * | 1997-02-20 | 2007-10-15 | Univ Johns Hopkins Med | Mutationen in atp-abhängigen transpositionsproteinen die die zielortspezifität reduzieren |
| WO2000026243A2 (en) * | 1998-10-29 | 2000-05-11 | Incyte Pharmaceuticals, Inc. | Transmembrane 4 proteins |
-
2001
- 2001-07-05 WO PCT/US2001/021269 patent/WO2002004674A2/en active IP Right Grant
- 2001-07-05 BR BRPI0112261-4A patent/BR0112261B1/pt not_active IP Right Cessation
- 2001-07-05 AT AT01952433T patent/ATE361994T1/de not_active IP Right Cessation
- 2001-07-05 EP EP01952433A patent/EP1327000B1/en not_active Expired - Lifetime
- 2001-07-05 JP JP2002509527A patent/JP4807921B2/ja not_active Expired - Fee Related
- 2001-07-05 CA CA002415786A patent/CA2415786C/en not_active Expired - Fee Related
- 2001-07-05 NZ NZ523507A patent/NZ523507A/en not_active IP Right Cessation
- 2001-07-05 AU AU7318501A patent/AU7318501A/xx active Pending
- 2001-07-05 AU AU2001273185A patent/AU2001273185B2/en not_active Ceased
- 2001-07-05 KR KR1020037000251A patent/KR100823857B1/ko not_active IP Right Cessation
- 2001-07-05 US US10/332,834 patent/US7229798B2/en not_active Expired - Lifetime
- 2001-07-05 MX MXPA03000038A patent/MXPA03000038A/es active IP Right Grant
- 2001-07-05 DE DE60128379T patent/DE60128379T2/de not_active Expired - Lifetime
- 2001-07-05 IL IL15379701A patent/IL153797A0/xx active IP Right Grant
- 2001-07-06 TW TW090116787A patent/TWI235180B/zh not_active IP Right Cessation
- 2001-07-06 AR ARP010103238A patent/AR029582A1/es active IP Right Grant
-
2002
- 2002-12-23 NO NO20026206A patent/NO329791B1/no not_active IP Right Cessation
-
2003
- 2003-01-02 ZA ZA200300035A patent/ZA200300035B/en unknown
- 2003-01-05 IL IL153797A patent/IL153797A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| IL153797A0 (en) | 2003-07-31 |
| MXPA03000038A (es) | 2003-08-19 |
| WO2002004674A3 (en) | 2003-04-17 |
| NZ523507A (en) | 2005-01-28 |
| AU7318501A (en) | 2002-01-21 |
| BR0112261B1 (pt) | 2012-10-02 |
| BR0112261A (pt) | 2003-09-02 |
| IL153797A (en) | 2008-06-05 |
| TWI235180B (en) | 2005-07-01 |
| ATE361994T1 (de) | 2007-06-15 |
| CA2415786C (en) | 2007-09-25 |
| DE60128379T2 (de) | 2008-01-10 |
| CA2415786A1 (en) | 2002-01-17 |
| US20030219779A1 (en) | 2003-11-27 |
| JP2004502467A (ja) | 2004-01-29 |
| EP1327000B1 (en) | 2007-05-09 |
| US7229798B2 (en) | 2007-06-12 |
| DE60128379D1 (de) | 2007-06-21 |
| AR029582A1 (es) | 2003-07-02 |
| NO20026206L (no) | 2003-02-25 |
| AU2001273185B2 (en) | 2006-05-18 |
| WO2002004674A2 (en) | 2002-01-17 |
| KR100823857B1 (ko) | 2008-04-21 |
| NO20026206D0 (no) | 2002-12-23 |
| JP4807921B2 (ja) | 2011-11-02 |
| KR20030021244A (ko) | 2003-03-12 |
| ZA200300035B (en) | 2004-03-15 |
| EP1327000A2 (en) | 2003-07-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |