AR029582A1 - Secuenciacion multiplex mediada por transposones - Google Patents
Secuenciacion multiplex mediada por transposonesInfo
- Publication number
- AR029582A1 AR029582A1 ARP010103238A ARP010103238A AR029582A1 AR 029582 A1 AR029582 A1 AR 029582A1 AR P010103238 A ARP010103238 A AR P010103238A AR P010103238 A ARP010103238 A AR P010103238A AR 029582 A1 AR029582 A1 AR 029582A1
- Authority
- AR
- Argentina
- Prior art keywords
- sequencing
- multiplex sequencing
- vector
- transmission multiplex
- efficiency
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Un método automatizado de secuenciacion multiplex mediada por transposones de fragmentos de ADN insertados en un vector. Se refiere, en forma particular, a un aumento en la eficiencia de tales métodos automatizados, donde el aumento de la eficiencia se obtiene a través de una seleccion antes de la secuenciacion de tales construcciones, en las que el transposon se inserto en la secuencia vectorial. Esto evita una pérdida de tiempo y recursos en la realizacion de reacciones de secuenciacion de vectores en lugar de los fragmentos de ADN de interés.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21638100P | 2000-07-07 | 2000-07-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR029582A1 true AR029582A1 (es) | 2003-07-02 |
Family
ID=22806831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP010103238A AR029582A1 (es) | 2000-07-07 | 2001-07-06 | Secuenciacion multiplex mediada por transposones |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US7229798B2 (es) |
| EP (1) | EP1327000B1 (es) |
| JP (1) | JP4807921B2 (es) |
| KR (1) | KR100823857B1 (es) |
| AR (1) | AR029582A1 (es) |
| AT (1) | ATE361994T1 (es) |
| AU (2) | AU7318501A (es) |
| BR (1) | BR0112261B1 (es) |
| CA (1) | CA2415786C (es) |
| DE (1) | DE60128379T2 (es) |
| IL (2) | IL153797A0 (es) |
| MX (1) | MXPA03000038A (es) |
| NO (1) | NO329791B1 (es) |
| NZ (1) | NZ523507A (es) |
| TW (1) | TWI235180B (es) |
| WO (1) | WO2002004674A2 (es) |
| ZA (1) | ZA200300035B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010126356A1 (en) * | 2009-04-29 | 2010-11-04 | Hendrix Genetics B.V. | Method of pooling samples for performing a biological assay |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2257109C (en) * | 1996-06-04 | 2009-10-06 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
| US6420524B1 (en) * | 1997-02-20 | 2002-07-16 | Johns Hopkins University School Of Medicine | Gain of function mutations in ATP-dependent transposition proteins |
| CA2347650A1 (en) * | 1998-10-29 | 2000-05-11 | Incyte Pharmaceuticals, Inc. | Transmembrane 4 proteins |
-
2001
- 2001-07-05 CA CA002415786A patent/CA2415786C/en not_active Expired - Fee Related
- 2001-07-05 BR BRPI0112261-4A patent/BR0112261B1/pt not_active IP Right Cessation
- 2001-07-05 AU AU7318501A patent/AU7318501A/xx active Pending
- 2001-07-05 US US10/332,834 patent/US7229798B2/en not_active Expired - Lifetime
- 2001-07-05 WO PCT/US2001/021269 patent/WO2002004674A2/en active IP Right Grant
- 2001-07-05 EP EP01952433A patent/EP1327000B1/en not_active Expired - Lifetime
- 2001-07-05 JP JP2002509527A patent/JP4807921B2/ja not_active Expired - Fee Related
- 2001-07-05 IL IL15379701A patent/IL153797A0/xx active IP Right Grant
- 2001-07-05 NZ NZ523507A patent/NZ523507A/en not_active IP Right Cessation
- 2001-07-05 AU AU2001273185A patent/AU2001273185B2/en not_active Ceased
- 2001-07-05 AT AT01952433T patent/ATE361994T1/de not_active IP Right Cessation
- 2001-07-05 DE DE60128379T patent/DE60128379T2/de not_active Expired - Lifetime
- 2001-07-05 MX MXPA03000038A patent/MXPA03000038A/es active IP Right Grant
- 2001-07-05 KR KR1020037000251A patent/KR100823857B1/ko not_active IP Right Cessation
- 2001-07-06 AR ARP010103238A patent/AR029582A1/es active IP Right Grant
- 2001-07-06 TW TW090116787A patent/TWI235180B/zh not_active IP Right Cessation
-
2002
- 2002-12-23 NO NO20026206A patent/NO329791B1/no not_active IP Right Cessation
-
2003
- 2003-01-02 ZA ZA200300035A patent/ZA200300035B/en unknown
- 2003-01-05 IL IL153797A patent/IL153797A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BR0112261A (pt) | 2003-09-02 |
| JP2004502467A (ja) | 2004-01-29 |
| NO20026206L (no) | 2003-02-25 |
| KR100823857B1 (ko) | 2008-04-21 |
| AU2001273185B2 (en) | 2006-05-18 |
| CA2415786C (en) | 2007-09-25 |
| KR20030021244A (ko) | 2003-03-12 |
| US20030219779A1 (en) | 2003-11-27 |
| MXPA03000038A (es) | 2003-08-19 |
| TWI235180B (en) | 2005-07-01 |
| NO329791B1 (no) | 2010-12-20 |
| JP4807921B2 (ja) | 2011-11-02 |
| EP1327000A2 (en) | 2003-07-16 |
| AU7318501A (en) | 2002-01-21 |
| NO20026206D0 (no) | 2002-12-23 |
| WO2002004674A2 (en) | 2002-01-17 |
| ZA200300035B (en) | 2004-03-15 |
| IL153797A (en) | 2008-06-05 |
| DE60128379T2 (de) | 2008-01-10 |
| US7229798B2 (en) | 2007-06-12 |
| CA2415786A1 (en) | 2002-01-17 |
| DE60128379D1 (de) | 2007-06-21 |
| ATE361994T1 (de) | 2007-06-15 |
| IL153797A0 (en) | 2003-07-31 |
| WO2002004674A3 (en) | 2003-04-17 |
| BR0112261B1 (pt) | 2012-10-02 |
| NZ523507A (en) | 2005-01-28 |
| EP1327000B1 (en) | 2007-05-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |