WO2005116213A3 - Directed evolution of proteins - Google Patents
Directed evolution of proteins Download PDFInfo
- Publication number
- WO2005116213A3 WO2005116213A3 PCT/US2005/013051 US2005013051W WO2005116213A3 WO 2005116213 A3 WO2005116213 A3 WO 2005116213A3 US 2005013051 W US2005013051 W US 2005013051W WO 2005116213 A3 WO2005116213 A3 WO 2005116213A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- proteins
- directed evolution
- fragments
- generate
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
Abstract
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US56276104P | 2004-04-15 | 2004-04-15 | |
US60/562,761 | 2004-04-15 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005116213A2 WO2005116213A2 (en) | 2005-12-08 |
WO2005116213A3 true WO2005116213A3 (en) | 2006-11-09 |
Family
ID=35451466
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2005/013051 WO2005116213A2 (en) | 2004-04-15 | 2005-04-15 | Directed evolution of proteins |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2005116213A2 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8932992B2 (en) | 2001-06-20 | 2015-01-13 | Nuevolution A/S | Templated molecules and methods for using such molecules |
US9096951B2 (en) | 2003-02-21 | 2015-08-04 | Nuevolution A/S | Method for producing second-generation library |
US9109248B2 (en) | 2002-10-30 | 2015-08-18 | Nuevolution A/S | Method for the synthesis of a bifunctional complex |
US9121110B2 (en) | 2002-12-19 | 2015-09-01 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
US9574189B2 (en) | 2005-12-01 | 2017-02-21 | Nuevolution A/S | Enzymatic encoding methods for efficient synthesis of large libraries |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60324778D1 (en) | 2002-03-15 | 2009-01-02 | Nuevolution As | AN IMPROVED METHOD FOR SYNTHESIS OF MATTRESS-RELATED MOLECULES |
AU2003247266A1 (en) | 2002-08-01 | 2004-02-23 | Nuevolution A/S | Multi-step synthesis of templated molecules |
ATE447626T1 (en) | 2003-09-18 | 2009-11-15 | Nuevolution As | METHOD FOR OBTAINING STRUCTURAL INFORMATION FROM ENCODED MOLECULES AND FOR SELECTING COMPOUNDS |
ES2713873T3 (en) | 2010-04-16 | 2019-05-24 | Nuevolution As | Bifunctional complexes and methods for making and using such complexes |
CN110872595B (en) * | 2018-08-31 | 2024-01-12 | 华南理工大学 | Acid-resistant expression cassette and application thereof in fermentation production of organic acid |
EP4054638A1 (en) * | 2019-11-08 | 2022-09-14 | The Trustees Of Columbia University In The City Of New York | Methods for engineering therapeutics and uses thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030027180A1 (en) * | 2001-03-19 | 2003-02-06 | Liu David R. | Nucleic acid shuffling |
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2005
- 2005-04-15 WO PCT/US2005/013051 patent/WO2005116213A2/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030027180A1 (en) * | 2001-03-19 | 2003-02-06 | Liu David R. | Nucleic acid shuffling |
Non-Patent Citations (6)
Title |
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BITTKER JOSHUA A ET AL: "Directed evolution of protein enzymes using nonhomologous random recombination", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 101, no. 18, 4 May 2004 (2004-05-04), pages 7011 - 7016, XP002376780, ISSN: 0027-8424 * |
BITTKER JOSHUA A ET AL: "Nucleic acid evolution and minimization by nonhomologous random recombination", NATURE BIOTECHNOLOGY, vol. 20, no. 10, October 2002 (2002-10-01), pages 1024 - 1029, XP002376779, ISSN: 1087-0156 * |
KAWARASAKI YASUAKI ET AL: "Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.", NUCLEIC ACIDS RESEARCH. 1 NOV 2003, vol. 31, no. 21, 1 November 2003 (2003-11-01), pages e126, XP002376777, ISSN: 1362-4962 * |
LUTZ S ET AL: "Creating multiple-crossover DNA libraries independent of sequence identity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 98, no. 20, 25 September 2001 (2001-09-25), pages 11248 - 11253, XP002250112, ISSN: 0027-8424 * |
LUTZ STEFAN ET AL: "Novel methods for directed evolution of enzymes: quality, not quantity", CURRENT OPINION IN BIOTECHNOLOGY, vol. 15, no. 4, August 2004 (2004-08-01), pages 291 - 297, XP002376781, ISSN: 0958-1669 * |
O'MAILLE PAUL E ET AL: "Structure-based combinatorial protein engineering (SCOPE).", JOURNAL OF MOLECULAR BIOLOGY, vol. 321, no. 4, 23 August 2002 (2002-08-23), pages 677 - 691, XP002376778, ISSN: 0022-2836 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8932992B2 (en) | 2001-06-20 | 2015-01-13 | Nuevolution A/S | Templated molecules and methods for using such molecules |
US9109248B2 (en) | 2002-10-30 | 2015-08-18 | Nuevolution A/S | Method for the synthesis of a bifunctional complex |
US9284600B2 (en) | 2002-10-30 | 2016-03-15 | Neuvolution A/S | Method for the synthesis of a bifunctional complex |
US9121110B2 (en) | 2002-12-19 | 2015-09-01 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
US9096951B2 (en) | 2003-02-21 | 2015-08-04 | Nuevolution A/S | Method for producing second-generation library |
US9574189B2 (en) | 2005-12-01 | 2017-02-21 | Nuevolution A/S | Enzymatic encoding methods for efficient synthesis of large libraries |
Also Published As
Publication number | Publication date |
---|---|
WO2005116213A2 (en) | 2005-12-08 |
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