NO329784B1 - Fremgangsmate for a redusere homologe rekombinasjonsfenomener, defekte rekombinante adenovirus og cellelinje. - Google Patents
Fremgangsmate for a redusere homologe rekombinasjonsfenomener, defekte rekombinante adenovirus og cellelinje. Download PDFInfo
- Publication number
- NO329784B1 NO329784B1 NO20002504A NO20002504A NO329784B1 NO 329784 B1 NO329784 B1 NO 329784B1 NO 20002504 A NO20002504 A NO 20002504A NO 20002504 A NO20002504 A NO 20002504A NO 329784 B1 NO329784 B1 NO 329784B1
- Authority
- NO
- Norway
- Prior art keywords
- sequence
- adenovirus
- region
- genome
- degenerate
- Prior art date
Links
- 230000002950 deficient Effects 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 42
- 241000701161 unidentified adenovirus Species 0.000 title claims description 85
- 230000006801 homologous recombination Effects 0.000 title claims description 39
- 238000002744 homologous recombination Methods 0.000 title claims description 39
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 67
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 63
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 63
- 238000004519 manufacturing process Methods 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 70
- 101150032643 IVa2 gene Proteins 0.000 claims description 36
- 230000004048 modification Effects 0.000 claims description 20
- 238000012986 modification Methods 0.000 claims description 20
- 108020004705 Codon Proteins 0.000 claims description 14
- 230000002759 chromosomal effect Effects 0.000 claims description 12
- 238000012217 deletion Methods 0.000 claims description 10
- 230000037430 deletion Effects 0.000 claims description 10
- 108091026890 Coding region Proteins 0.000 claims description 9
- 230000007850 degeneration Effects 0.000 claims description 5
- 230000007547 defect Effects 0.000 claims description 4
- 108700026244 Open Reading Frames Proteins 0.000 claims description 2
- 230000006798 recombination Effects 0.000 abstract description 43
- 238000005215 recombination Methods 0.000 abstract description 41
- 241000700605 Viruses Species 0.000 abstract description 30
- 230000003612 virological effect Effects 0.000 abstract description 15
- 239000002245 particle Substances 0.000 abstract description 11
- 230000010076 replication Effects 0.000 abstract description 8
- 210000004027 cell Anatomy 0.000 description 56
- 239000013612 plasmid Substances 0.000 description 32
- 101150057615 Syn gene Proteins 0.000 description 30
- 239000013598 vector Substances 0.000 description 25
- 230000014509 gene expression Effects 0.000 description 22
- 102000004169 proteins and genes Human genes 0.000 description 18
- 239000012634 fragment Substances 0.000 description 16
- 239000002773 nucleotide Substances 0.000 description 16
- 125000003729 nucleotide group Chemical group 0.000 description 16
- 108020004414 DNA Proteins 0.000 description 11
- 241001135569 Human adenovirus 5 Species 0.000 description 11
- 238000010276 construction Methods 0.000 description 10
- 230000001225 therapeutic effect Effects 0.000 description 10
- 230000003362 replicative effect Effects 0.000 description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 230000009467 reduction Effects 0.000 description 7
- 239000013603 viral vector Substances 0.000 description 7
- 125000003275 alpha amino acid group Chemical group 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000012795 verification Methods 0.000 description 6
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 101150066555 lacZ gene Proteins 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 241001430294 unidentified retrovirus Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 101100165660 Alternaria brassicicola bsc6 gene Proteins 0.000 description 2
- -1 ApoAIV Proteins 0.000 description 2
- 101100499295 Bacillus subtilis (strain 168) disA gene Proteins 0.000 description 2
- 101100243951 Caenorhabditis elegans pie-1 gene Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 241000702421 Dependoparvovirus Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000701533 Escherichia virus T4 Species 0.000 description 2
- 241000598171 Human adenovirus sp. Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 101150007210 ORF6 gene Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 101100226894 Phomopsis amygdali PaGT gene Proteins 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 108700005077 Viral Genes Proteins 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000005782 double-strand break Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000037432 silent mutation Effects 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 241001529453 unidentified herpesvirus Species 0.000 description 2
- 102100022464 5'-nucleotidase Human genes 0.000 description 1
- 208000010370 Adenoviridae Infections Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000007592 Apolipoproteins Human genes 0.000 description 1
- 108010071619 Apolipoproteins Proteins 0.000 description 1
- 102000013918 Apolipoproteins E Human genes 0.000 description 1
- 108010025628 Apolipoproteins E Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 1
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- 102100038909 Caveolin-2 Human genes 0.000 description 1
- 208000037088 Chromosome Breakage Diseases 0.000 description 1
- 108010005939 Ciliary Neurotrophic Factor Proteins 0.000 description 1
- 102100031614 Ciliary neurotrophic factor Human genes 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 102000000311 Cytosine Deaminase Human genes 0.000 description 1
- 108010080611 Cytosine Deaminase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000000655 Distemper Diseases 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 1
- 102100031706 Fibroblast growth factor 1 Human genes 0.000 description 1
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 102000004038 Glia Maturation Factor Human genes 0.000 description 1
- 108090000495 Glia Maturation Factor Proteins 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 108091027305 Heteroduplex Proteins 0.000 description 1
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 description 1
- 101000740981 Homo sapiens Caveolin-2 Proteins 0.000 description 1
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 1
- 101001055320 Myxine glutinosa Insulin-like growth factor Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000015336 Nerve Growth Factor Human genes 0.000 description 1
- 108090000742 Neurotrophin 3 Proteins 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 101710143509 Pre-histone-like nucleoprotein Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 101100379247 Salmo trutta apoa1 gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 102100032889 Sortilin Human genes 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000003914 blood derivative Substances 0.000 description 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
- 208000014058 canine distemper Diseases 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000009962 secretion pathway Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Battery Electrode And Active Subsutance (AREA)
- Control Of Motors That Do Not Use Commutators (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Ticket-Dispensing Machines (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9714383A FR2774699B1 (fr) | 1997-11-17 | 1997-11-17 | Methode de reduction des evenements de recombinaison homologue |
| PCT/FR1998/002453 WO1999025861A2 (fr) | 1997-11-17 | 1998-11-17 | Vecteurs adenoviraux et methode de reduction des evenements de recombinaison homologue |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO20002504L NO20002504L (no) | 2000-05-15 |
| NO20002504D0 NO20002504D0 (no) | 2000-05-15 |
| NO329784B1 true NO329784B1 (no) | 2010-12-20 |
Family
ID=9513447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20002504A NO329784B1 (no) | 1997-11-17 | 2000-05-15 | Fremgangsmate for a redusere homologe rekombinasjonsfenomener, defekte rekombinante adenovirus og cellelinje. |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US6410298B1 (xx) |
| EP (1) | EP1032695B1 (xx) |
| JP (1) | JP4376454B2 (xx) |
| KR (2) | KR100796060B1 (xx) |
| CN (1) | CN1201013C (xx) |
| AT (1) | ATE393234T1 (xx) |
| AU (1) | AU759531B2 (xx) |
| BR (1) | BR9814210A (xx) |
| CA (1) | CA2309315C (xx) |
| CZ (1) | CZ299797B6 (xx) |
| DE (1) | DE69839403T2 (xx) |
| FR (1) | FR2774699B1 (xx) |
| HU (1) | HU226018B1 (xx) |
| IL (2) | IL135983A0 (xx) |
| NO (1) | NO329784B1 (xx) |
| PL (1) | PL196606B1 (xx) |
| WO (1) | WO1999025861A2 (xx) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2799472B1 (fr) * | 1999-10-07 | 2004-07-16 | Aventis Pharma Sa | Preparation d'adenovirus recombinants et de banques adenovirales |
| US6821775B1 (en) * | 2000-02-11 | 2004-11-23 | Genvec, Inc. | Viral vector encoding pigment epithelium-derived factor |
| US7653769B2 (en) * | 2006-12-14 | 2010-01-26 | International Business Machines Corporation | Management of devices connected to infiniband ports |
| US6591543B2 (en) * | 2000-04-14 | 2003-07-15 | Kenneth P. Sabine | Top water lure with highly active propeller |
| KR20010064682A (ko) * | 2000-07-01 | 2001-07-11 | 김재호 | Ｅ1ｂ가 감쇠된 아데노바이러스를 이용한 ｃｄ／5－ｆｃ및 ｈｓｖ－1 ｔｋ／ｇｃｖ의 이중 자멸 유전자 시스템 |
| DE10045687B4 (de) * | 2000-09-15 | 2006-07-06 | MICROMUN Privates Institut für Mikrobiologische Forschung GmbH Biotechnikum Greifswald | Expressionskassetten und Adenovirusvektoren |
| US20030087438A1 (en) * | 2001-11-02 | 2003-05-08 | Genvec, Inc. | E1-revertant-free adenoviral composition |
| US20030158112A1 (en) | 2002-02-15 | 2003-08-21 | Johns Hopkins University School Of Medicine | Selective induction of apoptosis to treat ocular disease |
| JP2006516027A (ja) * | 2002-12-02 | 2006-06-15 | ジェンベク、インコーポレイティッド | 眼関連障害の治療用材料および治療方法 |
| EP1740218A1 (en) * | 2004-03-12 | 2007-01-10 | Genvec, Inc. | Materials for treating vascular leakage in the eye |
| US20060188481A1 (en) * | 2005-02-22 | 2006-08-24 | Saitama Medical School | Methods for prophylactically or therapeutically treating an animal for an ocular-related disorder |
| MX2009008118A (es) * | 2007-01-30 | 2009-10-13 | Transgene Sa | Polipeptido e2 del virus del papiloma usado para vacunacion. |
| RU2462513C2 (ru) * | 2007-05-15 | 2012-09-27 | Трансген С.А. | Векторы для множественной генной экспрессии |
| SI2173888T1 (sl) | 2007-07-26 | 2017-01-31 | Uniqure Ip B.V. | Bakulovirusni vektorji, ki vsebujejo ponavljajoča kodirna zaporedja z različnimi kodonskimi preferencami |
| CA2742247C (en) * | 2008-11-21 | 2017-07-04 | Bavarian Nordic A/S | Vector comprising multiple homologous nucleotide sequences |
| WO2010085660A2 (en) | 2009-01-23 | 2010-07-29 | Roger Williams Hospital | Viral vectors encoding multiple highly homologous non-viral polypeptides and the use of same |
| WO2012021730A2 (en) * | 2010-08-11 | 2012-02-16 | Genvec, Inc. | Respiratory syncytial virus (rsv) vaccine |
| EP2784155B1 (en) * | 2011-11-24 | 2018-06-13 | Viromed Co. Ltd. | Adenovirus producing novel cell line and the use thereof |
| US9014637B1 (en) * | 2013-09-27 | 2015-04-21 | Intel Corporation | Dynamic switching frequency control of an on-chip or integrated voltage regulator |
| CN108203706B (zh) * | 2017-11-30 | 2021-03-26 | 陕西师范大学 | 一种用于提高高容量腺病毒包装效率的辅助腺病毒 |
| CN112877358B (zh) * | 2021-02-07 | 2023-02-03 | 上海市第一人民医院 | 一种基于5型腺病毒序列的重组质粒及应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2726285B1 (fr) * | 1994-10-28 | 1996-11-29 | Centre Nat Rech Scient | Adenovirus depourvus de particules contaminantes viables, preparation et utilisation |
| ES2300105T3 (es) * | 1995-03-24 | 2008-06-01 | Genzyme Corporation | Vectores adenoviricos para terapia genica. |
| ATE278794T1 (de) * | 1995-06-15 | 2004-10-15 | Crucell Holland Bv | Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie |
-
1997
- 1997-11-17 FR FR9714383A patent/FR2774699B1/fr not_active Expired - Fee Related
-
1998
- 1998-11-17 CN CNB98810749XA patent/CN1201013C/zh not_active Expired - Fee Related
- 1998-11-17 CZ CZ20001791A patent/CZ299797B6/cs not_active IP Right Cessation
- 1998-11-17 KR KR1020007005333A patent/KR100796060B1/ko not_active IP Right Cessation
- 1998-11-17 BR BR9814210-0A patent/BR9814210A/pt not_active IP Right Cessation
- 1998-11-17 WO PCT/FR1998/002453 patent/WO1999025861A2/fr active IP Right Grant
- 1998-11-17 CA CA2309315A patent/CA2309315C/fr not_active Expired - Fee Related
- 1998-11-17 KR KR1020077020673A patent/KR20070103483A/ko not_active Application Discontinuation
- 1998-11-17 AT AT98955679T patent/ATE393234T1/de not_active IP Right Cessation
- 1998-11-17 PL PL340601A patent/PL196606B1/pl unknown
- 1998-11-17 US US09/530,378 patent/US6410298B1/en not_active Expired - Lifetime
- 1998-11-17 DE DE69839403T patent/DE69839403T2/de not_active Expired - Lifetime
- 1998-11-17 HU HU0101827A patent/HU226018B1/hu not_active IP Right Cessation
- 1998-11-17 JP JP2000521224A patent/JP4376454B2/ja not_active Expired - Fee Related
- 1998-11-17 EP EP98955679A patent/EP1032695B1/fr not_active Expired - Lifetime
- 1998-11-17 IL IL13598398A patent/IL135983A0/xx active IP Right Grant
-
2000
- 2000-05-03 AU AU31294/00A patent/AU759531B2/en not_active Ceased
- 2000-05-04 IL IL135983A patent/IL135983A/en not_active IP Right Cessation
- 2000-05-15 NO NO20002504A patent/NO329784B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR20010032146A (ko) | 2001-04-16 |
| WO1999025861A3 (fr) | 1999-07-01 |
| PL340601A1 (en) | 2001-02-12 |
| PL196606B1 (pl) | 2008-01-31 |
| CA2309315C (fr) | 2011-06-14 |
| JP4376454B2 (ja) | 2009-12-02 |
| CZ20001791A3 (cs) | 2000-08-16 |
| FR2774699A1 (fr) | 1999-08-13 |
| AU759531B2 (en) | 2003-04-17 |
| HUP0101827A2 (hu) | 2001-09-28 |
| EP1032695A2 (fr) | 2000-09-06 |
| KR100796060B1 (ko) | 2008-01-21 |
| AU3129400A (en) | 2000-12-14 |
| NO20002504L (no) | 2000-05-15 |
| HUP0101827A3 (en) | 2003-08-28 |
| ATE393234T1 (de) | 2008-05-15 |
| KR20070103483A (ko) | 2007-10-23 |
| NO20002504D0 (no) | 2000-05-15 |
| FR2774699B1 (fr) | 2003-10-03 |
| CZ299797B6 (cs) | 2008-11-26 |
| CN1201013C (zh) | 2005-05-11 |
| CA2309315A1 (fr) | 1999-05-27 |
| HU226018B1 (en) | 2008-03-28 |
| IL135983A0 (en) | 2001-05-20 |
| US6410298B1 (en) | 2002-06-25 |
| BR9814210A (pt) | 2000-10-03 |
| WO1999025861A2 (fr) | 1999-05-27 |
| IL135983A (en) | 2008-11-26 |
| DE69839403D1 (de) | 2008-06-05 |
| CN1278302A (zh) | 2000-12-27 |
| DE69839403T2 (de) | 2009-05-28 |
| JP2003504004A (ja) | 2003-02-04 |
| EP1032695B1 (fr) | 2008-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO329784B1 (no) | Fremgangsmate for a redusere homologe rekombinasjonsfenomener, defekte rekombinante adenovirus og cellelinje. | |
| Parks et al. | An enhanced system for construction of adenoviral vectors by the two-plasmid rescue method | |
| US6482617B2 (en) | Viable contaminant particle free adenoviruses, their preparation and use | |
| US6440728B1 (en) | Chimeric vectors comprising a phage packaging site and a portion derived from the genome of a eukaryotic virus | |
| JP3842818B2 (ja) | 組換えアデノウイルスゲノムの製造方法 | |
| NO320818B1 (no) | Rekombinant adenovirus av ikke-human, animalsk opprinnelse, farmasoytisk preparat omfattende dette samt anvendelse av adenoviruset. | |
| MXPA00012617A (es) | Adenovirus recombinante con replicacion defectuosa con un promotor tardio principal mutado. | |
| EP0988389A1 (en) | Method for the production of non-group c adenoviral vectors | |
| JP3827163B2 (ja) | 遺伝子治療用のアデノウイルスベクター | |
| Mittal et al. | Foreign gene expression by human adenovirus type 5-based vectors studied using firefly luciferase and bacterial β-galactosidase genes as reporters | |
| NO319055B1 (no) | Defekte, rekombinante adenoviruser med inaktivt IVa2-gen | |
| AU770005B2 (en) | Selective regulation of adenovirus production | |
| Lu et al. | Enhanced growth of recombinant human adenovirus type 41 (HAdV-41) carrying ADP gene | |
| Gall et al. | Rescue and production of vaccine and therapeutic adenovirus vectors expressing inhibitory transgenes | |
| AU785431B2 (en) | Recombinant adenoviruses preparation and adenovirus banks | |
| CN113774031B (zh) | 一种复制型人腺病毒及其应用 | |
| Xing et al. | Promoter activity of left inverted terminal repeat and downstream sequences of porcine adenovirus type 3 | |
| US7264818B2 (en) | BAV packaging regions and E1 transcriptional control regions | |
| US7687615B2 (en) | PAV regions for encapsidation and E1 transcriptional control | |
| AU2002313348B2 (en) | Method for preparing a recombinant adenovirus genome | |
| MXPA00004646A (en) | Adenovirus vectors and method for reducing homologous recombination phenomena |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CREP | Change of representative |
Representative=s name: EHRNER & DELMAR PATENTBYRA AB, BOX |
|
| CREP | Change of representative |
Representative=s name: ZACCO NORWAY AS, POSTBOKS 2003 VIKA |
|
| MM1K | Lapsed by not paying the annual fees |