NO318646B1 - Fremgangsmate for a detektere og analysere mikroorganismer samt testkit og reagens derfor - Google Patents
Fremgangsmate for a detektere og analysere mikroorganismer samt testkit og reagens derfor Download PDFInfo
- Publication number
- NO318646B1 NO318646B1 NO19970105A NO970105A NO318646B1 NO 318646 B1 NO318646 B1 NO 318646B1 NO 19970105 A NO19970105 A NO 19970105A NO 970105 A NO970105 A NO 970105A NO 318646 B1 NO318646 B1 NO 318646B1
- Authority
- NO
- Norway
- Prior art keywords
- adp
- atp
- stated
- sample
- adenylate kinase
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 61
- 244000005700 microbiome Species 0.000 title claims abstract description 35
- 238000003149 assay kit Methods 0.000 title abstract 2
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 claims abstract description 96
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 claims abstract description 96
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims abstract description 72
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims abstract description 72
- 108060001084 Luciferase Proteins 0.000 claims abstract description 63
- 239000005089 Luciferase Substances 0.000 claims abstract description 61
- 102000002281 Adenylate kinase Human genes 0.000 claims abstract description 60
- 108020000543 Adenylate kinase Proteins 0.000 claims abstract description 60
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims abstract description 38
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910001425 magnesium ion Inorganic materials 0.000 claims abstract description 38
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims abstract description 37
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims abstract description 37
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims abstract description 37
- 230000003834 intracellular effect Effects 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims description 28
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 23
- 229940098773 bovine serum albumin Drugs 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 23
- 239000003599 detergent Substances 0.000 claims description 23
- 210000004027 cell Anatomy 0.000 claims description 19
- 238000012360 testing method Methods 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 12
- 125000002091 cationic group Chemical group 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 239000011777 magnesium Substances 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 8
- 229910052749 magnesium Inorganic materials 0.000 claims description 8
- 230000029918 bioluminescence Effects 0.000 claims description 7
- 238000005415 bioluminescence Methods 0.000 claims description 7
- 239000000872 buffer Substances 0.000 claims description 6
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 claims description 6
- 239000011654 magnesium acetate Substances 0.000 claims description 6
- 235000011285 magnesium acetate Nutrition 0.000 claims description 6
- 229940069446 magnesium acetate Drugs 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 6
- 239000002738 chelating agent Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 230000002538 fungal effect Effects 0.000 claims description 3
- 238000004020 luminiscence type Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 239000007900 aqueous suspension Substances 0.000 claims description 2
- 210000001082 somatic cell Anatomy 0.000 claims description 2
- 238000011002 quantification Methods 0.000 claims 2
- 239000012898 sample dilution Substances 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 7
- 239000000523 sample Substances 0.000 description 38
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 229940088598 enzyme Drugs 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000004440 column chromatography Methods 0.000 description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 6
- 102000007347 Apyrase Human genes 0.000 description 5
- 108010007730 Apyrase Proteins 0.000 description 5
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229910000160 potassium phosphate Inorganic materials 0.000 description 3
- 235000011009 potassium phosphates Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 150000004985 diamines Chemical group 0.000 description 2
- 239000013024 dilution buffer Substances 0.000 description 2
- 239000005293 duran Substances 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 229960001596 famotidine Drugs 0.000 description 2
- SHFJWMWCIHQNCP-UHFFFAOYSA-M hydron;tetrabutylazanium;sulfate Chemical compound OS([O-])(=O)=O.CCCC[N+](CCCC)(CCCC)CCCC SHFJWMWCIHQNCP-UHFFFAOYSA-M 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 159000000003 magnesium salts Chemical class 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- 230000002407 ATP formation Effects 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 238000005460 biophysical method Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000000021 kinase assay Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB1994/001513 WO1996002665A1 (en) | 1994-07-13 | 1994-07-13 | Microbiological test method and reagents |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO970105D0 NO970105D0 (no) | 1997-01-10 |
| NO970105L NO970105L (no) | 1997-03-13 |
| NO318646B1 true NO318646B1 (no) | 2005-04-25 |
Family
ID=10749377
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19970105A NO318646B1 (no) | 1994-07-13 | 1997-01-10 | Fremgangsmate for a detektere og analysere mikroorganismer samt testkit og reagens derfor |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0788553B1 (de) |
| JP (1) | JP3856467B2 (de) |
| AT (1) | ATE215608T1 (de) |
| AU (1) | AU698916B2 (de) |
| BR (1) | BR9408598A (de) |
| CA (1) | CA2194457C (de) |
| DE (1) | DE69430327T2 (de) |
| DK (1) | DK0788553T3 (de) |
| ES (1) | ES2171457T3 (de) |
| GB (1) | GB2303919B (de) |
| HU (1) | HU220855B1 (de) |
| NO (1) | NO318646B1 (de) |
| NZ (1) | NZ268405A (de) |
| WO (1) | WO1996002665A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9308411D0 (en) * | 1993-04-23 | 1993-06-09 | Celsis Ltd | Detection of biological material |
| US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| JP2002500892A (ja) * | 1998-01-21 | 2002-01-15 | イギリス国 | 抗生物質感受性試験 |
| GB9803156D0 (en) * | 1998-02-13 | 1998-04-08 | Celsis Int Plc | Assay |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| GB9911095D0 (en) * | 1999-05-13 | 1999-07-14 | Secr Defence | Microbiological test method and reagents |
| GB9925161D0 (en) | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0122790D0 (en) | 2001-09-21 | 2001-11-14 | Secr Defence | Method of determining the presence of target bacteria |
| GB0202421D0 (en) * | 2002-02-01 | 2002-03-20 | Celsis Internat Plc | Polyols in bioluminescence assays |
| GB0508981D0 (en) | 2005-05-03 | 2005-06-08 | Acolyte Biomedica Ltd | Distinguishing cells in a sample |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| GB0915664D0 (en) | 2009-09-08 | 2009-10-07 | Enigma Diagnostics Ltd | Reaction method |
| JP2013537399A (ja) | 2010-04-16 | 2013-10-03 | ゼウス・サイエンティフィック・インコーポレイテッド | 非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法 |
| US9284500B2 (en) | 2013-03-14 | 2016-03-15 | Exxonmobil Research And Engineering Company | Production of base oils from petrolatum |
| WO2016040788A1 (en) | 2014-09-11 | 2016-03-17 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3933592A (en) * | 1965-02-17 | 1976-01-20 | Hazleton Laboratories, Incorporated | Method of detecting living microorganisms |
| DE3047860A1 (de) * | 1980-12-18 | 1982-07-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur bestimmung von hla-antigenen |
| CH678065A5 (en) * | 1988-04-13 | 1991-07-31 | Hamilton Bonaduz Ag | Quantitative determn. of adenosine-tri:phosphate - by a bio-luminescent reaction capable of determining ATP in somatic and/or microbial cells |
| GB9301118D0 (en) * | 1993-01-21 | 1993-03-10 | Secr Defence | Enzyme linked assays |
-
1994
- 1994-07-13 DE DE69430327T patent/DE69430327T2/de not_active Expired - Lifetime
- 1994-07-13 NZ NZ268405A patent/NZ268405A/en not_active IP Right Cessation
- 1994-07-13 BR BR9408598A patent/BR9408598A/pt not_active IP Right Cessation
- 1994-07-13 WO PCT/GB1994/001513 patent/WO1996002665A1/en active IP Right Grant
- 1994-07-13 GB GB9627142A patent/GB2303919B/en not_active Revoked
- 1994-07-13 DK DK94920556T patent/DK0788553T3/da active
- 1994-07-13 JP JP50477196A patent/JP3856467B2/ja not_active Expired - Lifetime
- 1994-07-13 CA CA002194457A patent/CA2194457C/en not_active Expired - Lifetime
- 1994-07-13 AU AU71305/94A patent/AU698916B2/en not_active Expired
- 1994-07-13 EP EP94920556A patent/EP0788553B1/de not_active Expired - Lifetime
- 1994-07-13 HU HU9700091A patent/HU220855B1/hu unknown
- 1994-07-13 ES ES94920556T patent/ES2171457T3/es not_active Expired - Lifetime
- 1994-07-13 AT AT94920556T patent/ATE215608T1/de active
-
1997
- 1997-01-10 NO NO19970105A patent/NO318646B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| GB2303919A (en) | 1997-03-05 |
| DE69430327T2 (de) | 2002-10-31 |
| DE69430327D1 (de) | 2002-05-08 |
| JP3856467B2 (ja) | 2006-12-13 |
| GB9627142D0 (en) | 1997-02-19 |
| EP0788553B1 (de) | 2002-04-03 |
| DK0788553T3 (da) | 2002-06-17 |
| NZ268405A (en) | 1997-12-19 |
| AU7130594A (en) | 1996-02-16 |
| CA2194457C (en) | 2009-05-12 |
| EP0788553A1 (de) | 1997-08-13 |
| HU220855B1 (en) | 2002-06-29 |
| HUT76563A (en) | 1997-09-29 |
| CA2194457A1 (en) | 1996-02-01 |
| WO1996002665A1 (en) | 1996-02-01 |
| NO970105D0 (no) | 1997-01-10 |
| NO970105L (no) | 1997-03-13 |
| ES2171457T3 (es) | 2002-09-16 |
| AU698916B2 (en) | 1998-11-12 |
| GB2303919B (en) | 1998-08-26 |
| BR9408598A (pt) | 1997-11-18 |
| JPH10502538A (ja) | 1998-03-10 |
| ATE215608T1 (de) | 2002-04-15 |
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