NO301175B1 - Fremgangsmåte for fremstilling av subenhetsprotein fra koleratoksin (CTB) eller hybrid CTB gen-fusjonsprotein - Google Patents

Fremgangsmåte for fremstilling av subenhetsprotein fra koleratoksin (CTB) eller hybrid CTB gen-fusjonsprotein Download PDF

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Publication number
NO301175B1
NO301175B1 NO893702A NO893702A NO301175B1 NO 301175 B1 NO301175 B1 NO 301175B1 NO 893702 A NO893702 A NO 893702A NO 893702 A NO893702 A NO 893702A NO 301175 B1 NO301175 B1 NO 301175B1
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NO
Norway
Prior art keywords
ctb
protein
gene
cholerae
cholera toxin
Prior art date
Application number
NO893702A
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English (en)
Norwegian (no)
Other versions
NO893702L (no
NO893702D0 (no
Inventor
Jan Holmgren
Joaquin Sanches Castillo
Original Assignee
Vitec Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitec Ab filed Critical Vitec Ab
Publication of NO893702D0 publication Critical patent/NO893702D0/no
Publication of NO893702L publication Critical patent/NO893702L/no
Publication of NO301175B1 publication Critical patent/NO301175B1/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/55Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Soy Sauces And Products Related Thereto (AREA)
  • Exhaust Gas Treatment By Means Of Catalyst (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
NO893702A 1988-09-16 1989-09-15 Fremgangsmåte for fremstilling av subenhetsprotein fra koleratoksin (CTB) eller hybrid CTB gen-fusjonsprotein NO301175B1 (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8803291A SE462285B (sv) 1988-09-16 1988-09-16 Expression av koleratoxinets bindande subenhet med hjaelp av fraemmande promotor- och/eller ledarpeptidstrukturer

Publications (3)

Publication Number Publication Date
NO893702D0 NO893702D0 (no) 1989-09-15
NO893702L NO893702L (no) 1990-03-19
NO301175B1 true NO301175B1 (no) 1997-09-22

Family

ID=20373364

Family Applications (1)

Application Number Title Priority Date Filing Date
NO893702A NO301175B1 (no) 1988-09-16 1989-09-15 Fremgangsmåte for fremstilling av subenhetsprotein fra koleratoksin (CTB) eller hybrid CTB gen-fusjonsprotein

Country Status (13)

Country Link
EP (1) EP0368819B1 (sv)
JP (1) JP2790333B2 (sv)
AT (1) ATE161885T1 (sv)
AU (1) AU633842B2 (sv)
CA (1) CA1340577C (sv)
DE (1) DE68928529T2 (sv)
DK (1) DK175694B1 (sv)
ES (1) ES2113342T3 (sv)
FI (1) FI104496B (sv)
GR (1) GR3025846T3 (sv)
IS (1) IS1804B (sv)
NO (1) NO301175B1 (sv)
SE (1) SE462285B (sv)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0416505A3 (en) * 1989-09-04 1991-10-02 Takeda Chemical Industries, Ltd. Expression plasmids and use thereof
US5356797A (en) * 1991-11-15 1994-10-18 Board Of Regents, The University Of Texas Membrane expression of heterologous genes
EP0613500A1 (en) * 1991-11-15 1994-09-07 The Board Of Regents, The University Of Texas System Membrane expression of heterologous genes
US5932714A (en) * 1995-02-23 1999-08-03 Connaught Laboratories Limited Expression of gene products from genetically manipulated strains of Bordetella
US5998168A (en) * 1995-06-07 1999-12-07 Connaught Laboratories Limited Expression of gene products from genetically manipulated strains of bordetella
US6008329A (en) * 1998-03-06 1999-12-28 The United States Of America As Represented By The Secretary Of The Army Method for purifying cholera toxin
WO2007117339A2 (en) * 2006-01-11 2007-10-18 The United States Of America As Respresented By The Secretary Of The Navy Adhesin-enterotoxin chimera based immunogenic composition against entertoxigenic escherichia coli
DE102011111688A1 (de) * 2011-08-24 2013-02-28 Bioserv Analytik Und Medizinprodukte Gmbh Verfahren zur schnellen Isolation bzw. Aufreinigung von rekombinanten Proteinen mit einer (nicht toxischen) Choleratoxin-Domäne
CN109096403B (zh) * 2018-07-04 2020-06-30 浙江工商大学 一种用于蛋白质转导的蛋白质载体及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666837A (en) * 1982-05-24 1987-05-19 Smithkline-Rit DNA sequences, recombinant DNA molecules and processes for producing the A and B subunits of cholera toxin and preparations containing so-obtained subunit or subunits
US5135862A (en) * 1983-03-04 1992-08-04 University Of Maryland Method of isolating restriction fragment deletions in vibrio cholerae, products thereof
US4882278A (en) * 1983-04-29 1989-11-21 President And Fellows Of Harvard College Non-toxinogenic vibrio cholerae mutants
EP0251579A3 (en) * 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Non-antibiotic marker system
EP0358692B1 (en) * 1987-04-29 1998-06-03 President And Fellows Of Harvard College Cholera vaccines

Also Published As

Publication number Publication date
SE462285B (sv) 1990-05-28
ATE161885T1 (de) 1998-01-15
NO893702L (no) 1990-03-19
EP0368819B1 (en) 1998-01-07
FI894368A (fi) 1990-03-17
ES2113342T3 (es) 1998-05-01
JP2790333B2 (ja) 1998-08-27
NO893702D0 (no) 1989-09-15
FI894368A0 (fi) 1989-09-15
CA1340577C (en) 1999-06-01
DE68928529T2 (de) 1998-04-16
IS3505A7 (is) 1990-03-17
DK456989D0 (da) 1989-09-15
DE68928529D1 (de) 1998-02-12
EP0368819A1 (en) 1990-05-16
DK175694B1 (da) 2005-01-24
GR3025846T3 (en) 1998-04-30
AU633842B2 (en) 1993-02-11
DK456989A (da) 1990-03-17
IS1804B (is) 2002-02-26
JPH0322997A (ja) 1991-01-31
SE8803291D0 (sv) 1988-09-16
AU4134989A (en) 1990-04-05
FI104496B (sv) 2000-02-15

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