NO300428B1 - Fremgangsmåte ved fremstilling av proteiner med FVIII aktivitet eller FVIII derivater - Google Patents
Fremgangsmåte ved fremstilling av proteiner med FVIII aktivitet eller FVIII derivater Download PDFInfo
- Publication number
- NO300428B1 NO300428B1 NO920080A NO920080A NO300428B1 NO 300428 B1 NO300428 B1 NO 300428B1 NO 920080 A NO920080 A NO 920080A NO 920080 A NO920080 A NO 920080A NO 300428 B1 NO300428 B1 NO 300428B1
- Authority
- NO
- Norway
- Prior art keywords
- fviii
- cells
- temperature
- hours
- heavy chain
- Prior art date
Links
- 230000000694 effects Effects 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims description 9
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 title claims description 8
- 102000004169 proteins and genes Human genes 0.000 title claims description 8
- 108090000623 proteins and genes Proteins 0.000 title claims description 8
- 102100026735 Coagulation factor VIII Human genes 0.000 title claims description 7
- 230000008569 process Effects 0.000 title claims description 3
- 210000004027 cell Anatomy 0.000 claims description 22
- 239000013612 plasmid Substances 0.000 claims description 14
- 210000004962 mammalian cell Anatomy 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 4
- 108010054218 Factor VIII Proteins 0.000 description 53
- 102000001690 Factor VIII Human genes 0.000 description 53
- 229960000301 factor viii Drugs 0.000 description 53
- 239000002609 medium Substances 0.000 description 13
- 239000012679 serum free medium Substances 0.000 description 6
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 4
- 241000282693 Cercopithecidae Species 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 230000036284 oxygen consumption Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 101150074155 DHFR gene Proteins 0.000 description 2
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 201000003542 Factor VIII deficiency Diseases 0.000 description 2
- 208000009292 Hemophilia A Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010022394 Threonine synthase Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000002301 combined effect Effects 0.000 description 2
- 108020001096 dihydrofolate reductase Proteins 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 108010047303 von Willebrand Factor Proteins 0.000 description 2
- 102100036537 von Willebrand factor Human genes 0.000 description 2
- 229960001134 von willebrand factor Drugs 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001045988 Neogene Species 0.000 description 1
- CWRVKFFCRWGWCS-UHFFFAOYSA-N Pentrazole Chemical compound C1CCCCC2=NN=NN21 CWRVKFFCRWGWCS-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000012761 co-transfection Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 102000057593 human F8 Human genes 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 101150091879 neo gene Proteins 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/DK1989/000168 WO1991000347A1 (en) | 1989-07-04 | 1989-07-04 | Method of producing proteins with fviii activity and/or fviii derivatives |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO920080D0 NO920080D0 (no) | 1992-01-06 |
| NO920080L NO920080L (no) | 1992-01-06 |
| NO300428B1 true NO300428B1 (no) | 1997-05-26 |
Family
ID=8153554
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO920080A NO300428B1 (no) | 1989-07-04 | 1992-01-06 | Fremgangsmåte ved fremstilling av proteiner med FVIII aktivitet eller FVIII derivater |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0480923B1 (sv) |
| JP (1) | JP2810744B2 (sv) |
| FI (1) | FI94148C (sv) |
| NO (1) | NO300428B1 (sv) |
| WO (1) | WO1991000347A1 (sv) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4306813B2 (ja) | 1995-09-19 | 2009-08-05 | アスビオファーマ株式会社 | 動物細胞の新規培養方法 |
| TR200504220T2 (tr) | 1998-12-17 | 2007-04-24 | Biogen Idec Ma Inc. | Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem. |
| EP2073833A2 (en) | 2006-10-20 | 2009-07-01 | Biogen Idec MA Inc. | Treatment of demyelinating disorders with soluble lymphotoxin-beta-receptor |
| US8338376B2 (en) | 2006-10-20 | 2012-12-25 | Biogen Idec Ma Inc. | Compositions comprising variant LT-B-R-IG fusion proteins |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL84168A0 (en) * | 1986-10-15 | 1988-03-31 | Rorer Int Overseas | Human factor viii-c analogs,process for the preparation thereof and pharmaceutical compositions containing the same |
-
1989
- 1989-07-04 EP EP89908155A patent/EP0480923B1/en not_active Expired - Lifetime
- 1989-07-04 JP JP1507715A patent/JP2810744B2/ja not_active Expired - Lifetime
- 1989-07-04 WO PCT/DK1989/000168 patent/WO1991000347A1/en active IP Right Grant
-
1992
- 1992-01-03 FI FI920037A patent/FI94148C/sv active
- 1992-01-06 NO NO920080A patent/NO300428B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04506448A (ja) | 1992-11-12 |
| EP0480923B1 (en) | 1993-09-29 |
| WO1991000347A1 (en) | 1991-01-10 |
| NO920080D0 (no) | 1992-01-06 |
| FI94148C (sv) | 1995-07-25 |
| NO920080L (no) | 1992-01-06 |
| EP0480923A1 (en) | 1992-04-22 |
| JP2810744B2 (ja) | 1998-10-15 |
| FI920037A0 (sv) | 1992-01-03 |
| FI94148B (sv) | 1995-04-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK1K | Patent expired |