NO175266C - Fremgangsmåte for fremstilling av et humant t-PA protein som mangler en eller flere av de N-bundne karbohydratrestene, DNA, hybridvektor samt transformert eukaryot vertscelle som er i stand til å uttrykke et humant t-PA protein - Google Patents

Fremgangsmåte for fremstilling av et humant t-PA protein som mangler en eller flere av de N-bundne karbohydratrestene, DNA, hybridvektor samt transformert eukaryot vertscelle som er i stand til å uttrykke et humant t-PA protein

Info

Publication number
NO175266C
NO175266C NO864569A NO864569A NO175266C NO 175266 C NO175266 C NO 175266C NO 864569 A NO864569 A NO 864569A NO 864569 A NO864569 A NO 864569A NO 175266 C NO175266 C NO 175266C
Authority
NO
Norway
Prior art keywords
human
protein
expressing
dna
producing
Prior art date
Application number
NO864569A
Other languages
English (en)
Norwegian (no)
Other versions
NO864569D0 (no
NO175266B (no
NO864569L (no
Inventor
Bhabatosh Chaudhuri
Bernd Meyhack
Jutta Heim
Jan Van Oostrum
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of NO864569D0 publication Critical patent/NO864569D0/no
Publication of NO864569L publication Critical patent/NO864569L/no
Publication of NO175266B publication Critical patent/NO175266B/no
Publication of NO175266C publication Critical patent/NO175266C/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
NO864569A 1985-11-18 1986-11-17 Fremgangsmåte for fremstilling av et humant t-PA protein som mangler en eller flere av de N-bundne karbohydratrestene, DNA, hybridvektor samt transformert eukaryot vertscelle som er i stand til å uttrykke et humant t-PA protein NO175266C (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB858528321A GB8528321D0 (en) 1985-11-18 1985-11-18 Modified fibrinolytic agents

Publications (4)

Publication Number Publication Date
NO864569D0 NO864569D0 (no) 1986-11-17
NO864569L NO864569L (no) 1987-05-19
NO175266B NO175266B (no) 1994-06-13
NO175266C true NO175266C (no) 1994-09-28

Family

ID=10588363

Family Applications (1)

Application Number Title Priority Date Filing Date
NO864569A NO175266C (no) 1985-11-18 1986-11-17 Fremgangsmåte for fremstilling av et humant t-PA protein som mangler en eller flere av de N-bundne karbohydratrestene, DNA, hybridvektor samt transformert eukaryot vertscelle som er i stand til å uttrykke et humant t-PA protein

Country Status (17)

Country Link
EP (1) EP0225286B1 (xx)
JP (1) JPS62272976A (xx)
KR (1) KR920007666B1 (xx)
AT (1) ATE78514T1 (xx)
AU (1) AU598490B2 (xx)
DE (1) DE3686136T2 (xx)
DK (1) DK174977B1 (xx)
ES (1) ES2043607T3 (xx)
FI (1) FI91160C (xx)
GB (1) GB8528321D0 (xx)
GR (1) GR3006041T3 (xx)
IE (1) IE57866B1 (xx)
IL (1) IL80659A (xx)
NO (1) NO175266C (xx)
NZ (1) NZ218305A (xx)
PT (1) PT83752B (xx)
ZA (1) ZA868689B (xx)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ213647A (en) * 1984-10-01 1989-05-29 Integrated Genetics Inc Recombinant human tissue plasminogen activator (tpa) modified in n-linked glycosylation pattern
ATE74379T1 (de) * 1985-12-23 1992-04-15 Chiron Corp Peptidplasminogenaktivatoren.
US5002887A (en) * 1986-01-31 1991-03-26 Genetics Institute, Inc. Truncated thrombolytic proteins
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
US5589361A (en) * 1986-03-18 1996-12-31 Genentech, Inc. Human tissue-type plasminogen activator variant
US5047241A (en) * 1986-07-11 1991-09-10 American Home Products Corporation Tris-Kringle plasminogen activator with novel K2 insert
EP0276846A3 (en) * 1987-01-29 1989-07-26 Zymogenetics, Inc. Colony-stimulating factor derivatives
JPH084501B2 (ja) * 1987-03-20 1996-01-24 エーザイ株式会社 糖鎖に関する変異ｔＰＡ
SE8702562L (sv) * 1987-06-18 1988-12-19 Kabigen Ab Nya fibrinolytiska enzymer
US6682733B1 (en) * 1987-06-18 2004-01-27 Roche Diagnostics, Gmbh Fibrinolytic enzymes
US5266474A (en) * 1987-06-24 1993-11-30 Genentech, Inc. Balanced inducible transcription system
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
WO1989000191A1 (en) * 1987-07-06 1989-01-12 Genetics Institute, Inc. Novel thrombolytic proteins
AU1893988A (en) * 1987-07-13 1989-01-19 Collaborative Research Inc. Nonglycosylated plasminogen activator and method of preparation
US5270198A (en) * 1988-05-20 1993-12-14 Genentech, Inc. DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
US5346824A (en) * 1988-05-20 1994-09-13 Genentech, Inc. DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof
IL90146A (en) * 1988-05-20 1994-10-07 Genentech Inc Glycosylation derivatives of tissue plasminogen activator
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
WO1991015235A1 (en) * 1990-03-30 1991-10-17 Survival Technology, Inc. t-PA ENHANCER PROFILE
US5242688A (en) * 1990-12-24 1993-09-07 Eli Lilly And Company Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
NZ271088A (en) * 1993-08-04 1996-10-28 Ciba Geigy Ag Desulphatohirudin muteins, their production, conjugates thereof and pharmaceutical compositions
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
WO2013188587A1 (en) * 2012-06-14 2013-12-19 Portola Pharmaceuticals, Inc. METHOD FOR PURIFICATION OF RECOMBINANT FACTOR Xa DERIVATIVES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79202B (xx) * 1982-05-05 1984-10-22 Genentech Inc
KR840004781A (ko) * 1982-05-05 1984-10-24 월터 에이치·드레거 인체의 조직 플라스미노겐 활성화제
WO1984001786A1 (en) * 1982-10-28 1984-05-10 Beecham Group Plc Enzyme derivatives and their use in the treatment of thrombosis
IE56716B1 (en) * 1983-01-19 1991-11-20 Genentech Inc Method for producing human tpa and expression vectors therefor
NZ213647A (en) * 1984-10-01 1989-05-29 Integrated Genetics Inc Recombinant human tissue plasminogen activator (tpa) modified in n-linked glycosylation pattern
DE3537176C2 (de) * 1984-10-18 1994-06-09 Zymogenetics Inc Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
JPH0811066B2 (ja) * 1985-09-30 1996-02-07 インテグレーテッド・ジエネティックス・インコーポレーテッド 組換えｄｎａが生産するヒト子宮プラスミノ−ゲンアクチベ−タ−

Also Published As

Publication number Publication date
JPS62272976A (ja) 1987-11-27
DK549186A (da) 1987-05-19
NO864569D0 (no) 1986-11-17
AU598490B2 (en) 1990-06-28
EP0225286B1 (en) 1992-07-22
PT83752B (pt) 1989-06-30
ES2043607T3 (es) 1994-01-01
IL80659A0 (en) 1987-02-27
NO175266B (no) 1994-06-13
FI864654A (fi) 1987-05-19
IL80659A (en) 1991-12-15
EP0225286A3 (en) 1987-10-07
DE3686136T2 (de) 1992-12-17
FI864654A0 (fi) 1986-11-14
DK174977B1 (da) 2004-04-05
NO864569L (no) 1987-05-19
ZA868689B (en) 1987-06-24
KR920007666B1 (ko) 1992-09-14
FI91160C (fi) 1994-05-25
DE3686136D1 (de) 1992-08-27
AU6520086A (en) 1987-05-21
DK549186D0 (da) 1986-11-17
GR3006041T3 (xx) 1993-06-21
PT83752A (en) 1986-12-01
GB8528321D0 (en) 1985-12-24
IE57866B1 (en) 1993-05-05
ATE78514T1 (de) 1992-08-15
IE863028L (en) 1987-05-18
FI91160B (fi) 1994-02-15
NZ218305A (en) 1990-01-29
KR870005094A (ko) 1987-06-04
EP0225286A2 (en) 1987-06-10

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