NL2035090A - Maize Dual Regulatory Module for Regulating the Balance between Plant Growth and Disease Resistance - Google Patents
Maize Dual Regulatory Module for Regulating the Balance between Plant Growth and Disease Resistance Download PDFInfo
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- NL2035090A NL2035090A NL2035090A NL2035090A NL2035090A NL 2035090 A NL2035090 A NL 2035090A NL 2035090 A NL2035090 A NL 2035090A NL 2035090 A NL2035090 A NL 2035090A NL 2035090 A NL2035090 A NL 2035090A
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Claims (4)
1. Toepassing van een dubbele regelmodule van mais voor het reguleren van de balans tussen plantengroei en ziekteresistentie, waarbij: — de dubbele regelmodule van mais een promoter van het ZmTBF1a-gen en zijn UORF- sequentie bevat, namelijk pZmTBF 1a: uUORFZmTBF 1a, waarvan de nucleotidensequentie wordt getoond in SEQ ID No.1; — de dubbele regelmodule van mais tevens een promoter van het ZmTBF1b-gen en een UORF-sequentie, namelijk pZmTBF1b: YORF?"73F1° omvat, getoond in SEQ ID No.2; — de toepassing het verbinden van ZmTBF 1a stroomafwaarts van pZmTBF 1a: UORFZ"78F12 en van ZmTBF1b stroomafwaarts van pZmTBF1b: uORF?"78F%% omvat; waarbij de nucleotidensequentie van ZmTBF1a wordt getoond in SEQ ID No.3 en de nucleotidensequentie van ZmTBF1b wordt getoond in SEQ ID No.4; — de plant mais is; — de ziekteresistentie verwijst naar de resistentie tegen ziekten veroorzaakt door stammen van Puccinia polysora en Curvularia lunata.
2. Toepassing van mais dubbele regelmodule voor het reguleren van de balans tussen plantengroei en ziekteresistentie volgens conclusie 1, waarbij het VORF ZmTBF1 element en de overeenkomstige ZmTBF1 promotorsequentie vermeerderd zijn uit maïs-DNA en vervolgens door naadloze klonering in plantenexpressievector pCAMBIA3301 zijn gekloneerd.
3. Toepassing van maïs dubbele regelmodule voor het reguleren van de balans tussen plantengroei en ziekteresistentie volgens conclusie 2, waarbij de primers voor het vermeerderen van het uORF?"'BF element en de overeenkomstige ZmTBF1 promotorsequentie als volgt zijn: ZmTBF1a-3301: voorwaarts: 5'-atgaccatgattacgaattcCATCGCCATCCATTTAAACATT-3' (SEQ ID NO:29) terugwaarts: 5'-taccctcagatctaccatggCCCTCGCCGGCTATGTATA-3' (SEQ ID NO:30) ZmTBF1b-3301: voorwaarts:5'-atgaccatgattacgaattcGCTCGCTCGATCGTCCGCCCTT-3' (SEQ ID NO:31) terugwaarts:5'-taccctcagatctaccatggCTCTCGCTCGGCGCCGACGATT-3' (SEQ ID NO:32).
4. Toepassing van mais dubbele regelmodule voor het reguleren van de balans tussen plantengroei en ziekteresistentie volgens conclusie 2, waarbij de vector pCAMBIA3301 is gelineariseerd door gebruik te maken van EcoRl en Ncol restrictie-endonucleasen, en de vermeerderde dubbele regelmodule van mais door naadloze kloneringstechnologie verbonden is met de plant-expressievector.
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