NL2034776B1 - Primer set for triplex rt-pcr detection of porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), and porcine delta coronavirus (pdcov), and use thereof - Google Patents
Primer set for triplex rt-pcr detection of porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), and porcine delta coronavirus (pdcov), and use thereof Download PDFInfo
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- NL2034776B1 NL2034776B1 NL2034776A NL2034776A NL2034776B1 NL 2034776 B1 NL2034776 B1 NL 2034776B1 NL 2034776 A NL2034776 A NL 2034776A NL 2034776 A NL2034776 A NL 2034776A NL 2034776 B1 NL2034776 B1 NL 2034776B1
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- pdcov
- tgev
- pedv
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- porcine
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- 241001361508 Porcine deltacoronavirus Species 0.000 title claims abstract description 66
- 241001135549 Porcine epidemic diarrhea virus Species 0.000 title claims abstract description 66
- 241000711484 Transmissible gastroenteritis virus Species 0.000 title claims abstract description 66
- 238000001514 detection method Methods 0.000 title claims abstract description 62
- 238000003757 reverse transcription PCR Methods 0.000 claims abstract description 42
- 239000000523 sample Substances 0.000 claims description 75
- 239000013612 plasmid Substances 0.000 claims description 41
- 230000003321 amplification Effects 0.000 claims description 28
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 16
- 239000013641 positive control Substances 0.000 claims description 13
- 239000012634 fragment Substances 0.000 claims description 12
- 239000013642 negative control Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 9
- 239000013558 reference substance Substances 0.000 claims description 6
- 108700026220 vif Genes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 238000012408 PCR amplification Methods 0.000 claims description 4
- 101150010882 S gene Proteins 0.000 claims description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 claims description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 102100034343 Integrase Human genes 0.000 claims description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims 1
- 230000000741 diarrhetic effect Effects 0.000 claims 1
- 239000013589 supplement Substances 0.000 claims 1
- 206010012735 Diarrhoea Diseases 0.000 abstract description 16
- 244000052769 pathogen Species 0.000 abstract description 13
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract 2
- 238000003748 differential diagnosis Methods 0.000 abstract 1
- 238000003771 laboratory diagnosis Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 20
- 108020004707 nucleic acids Proteins 0.000 description 12
- 150000007523 nucleic acids Chemical class 0.000 description 12
- 102000039446 nucleic acids Human genes 0.000 description 12
- 238000011529 RT qPCR Methods 0.000 description 9
- 108091006146 Channels Proteins 0.000 description 8
- 241000700605 Viruses Species 0.000 description 8
- 241001673669 Porcine circovirus 2 Species 0.000 description 7
- 241000702665 Porcine rotavirus Species 0.000 description 7
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 7
- 239000012895 dilution Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 241000711573 Coronaviridae Species 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 208000003322 Coinfection Diseases 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 230000001502 supplementing effect Effects 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 101150096544 AM gene Proteins 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 208000005577 Gastroenteritis Diseases 0.000 description 2
- 241000582786 Monoplex Species 0.000 description 2
- 206010051511 Viral diarrhoea Diseases 0.000 description 2
- 201000009840 acute diarrhea Diseases 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
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- 231100000915 pathological change Toxicity 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 101001047090 Homo sapiens Potassium voltage-gated channel subfamily H member 2 Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000353097 Molva molva Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 102100022807 Potassium voltage-gated channel subfamily H member 2 Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 244000000023 zoonotic pathogen Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211253507.9A CN116042917A (zh) | 2022-10-13 | 2022-10-13 | 一种检测猪流行性腹泻、传染性胃肠炎和丁型冠状病毒的三重rt-pcr引物组及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2034776B1 true NL2034776B1 (en) | 2024-05-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2034776A NL2034776B1 (en) | 2022-10-13 | 2023-05-08 | Primer set for triplex rt-pcr detection of porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), and porcine delta coronavirus (pdcov), and use thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN116042917A (zh) |
| NL (1) | NL2034776B1 (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117701779A (zh) * | 2024-02-04 | 2024-03-15 | 湖南派智生物科技有限公司 | 鉴别非洲猪瘟毒株的方法、引物探针组合、试剂、试剂盒及应用 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257557A (zh) * | 2019-06-10 | 2019-09-20 | 华南农业大学 | 一种TGEV、PEDV、SADS-CoV和PDCoV的多重RT-PCR检测引物组 |
| CN111500791A (zh) * | 2020-06-11 | 2020-08-07 | 河南省动物疫病预防控制中心 | 猪流行性腹泻病毒、猪δ冠状病毒和猪急性腹泻综合征冠状病毒三重FQ-PCR检测方法 |
| CN113462820A (zh) * | 2021-07-21 | 2021-10-01 | 河北工程大学 | 实时荧光定量检测四种猪腹泻病毒的多重rt-pcr引物探针组、试剂盒及其检测方法 |
| CN113943831A (zh) * | 2021-10-14 | 2022-01-18 | 泰州蕾灵百奥生物科技有限公司 | 可同时诊断猪腹泻病三种高发病原的多重荧光定量引物和探针组合及其应用 |
-
2022
- 2022-10-13 CN CN202211253507.9A patent/CN116042917A/zh active Pending
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2023
- 2023-05-08 NL NL2034776A patent/NL2034776B1/en active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257557A (zh) * | 2019-06-10 | 2019-09-20 | 华南农业大学 | 一种TGEV、PEDV、SADS-CoV和PDCoV的多重RT-PCR检测引物组 |
| CN111500791A (zh) * | 2020-06-11 | 2020-08-07 | 河南省动物疫病预防控制中心 | 猪流行性腹泻病毒、猪δ冠状病毒和猪急性腹泻综合征冠状病毒三重FQ-PCR检测方法 |
| CN113462820A (zh) * | 2021-07-21 | 2021-10-01 | 河北工程大学 | 实时荧光定量检测四种猪腹泻病毒的多重rt-pcr引物探针组、试剂盒及其检测方法 |
| CN113943831A (zh) * | 2021-10-14 | 2022-01-18 | 泰州蕾灵百奥生物科技有限公司 | 可同时诊断猪腹泻病三种高发病原的多重荧光定量引物和探针组合及其应用 |
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| Publication number | Publication date |
|---|---|
| CN116042917A (zh) | 2023-05-02 |
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