NL2032234B1 - Active probiotics compositions preparation method - Google Patents

Active probiotics compositions preparation method Download PDF

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NL2032234B1
NL2032234B1 NL2032234A NL2032234A NL2032234B1 NL 2032234 B1 NL2032234 B1 NL 2032234B1 NL 2032234 A NL2032234 A NL 2032234A NL 2032234 A NL2032234 A NL 2032234A NL 2032234 B1 NL2032234 B1 NL 2032234B1
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powder
active
lactobacillus
preparation
mixture
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NL2032234A
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Gao Qian
Ye Qingcai
Liu Junhui
Liu Hongyan
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Anhui Zhongwei Weiyuan Biological Tech Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera

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Abstract

Active Probiotics Compositions Preparation Method A method of preparing active probiotics compositions is disclosed, beginning with the 5 preparation of lactobacillus acidophilus, active lactobacillus curvatus powder and active lactobacillus rhamnosus powder respectively, then the addition of aloe juice dilution, lactobacillus acidophilus powder, active lactobacillus crispatus powder, active lactobacillus rhamnosus powder, pearl powder and osmanthus fragrans fructooligosaccharide in turn through a sterile mixing bucket with intensive mixing for the mixture, followed by the 10 freeze-drying and concentration of the mixture by vacuum freeze-dryer, then being crushed by crusher to get probiotics compositions powder, finally filtering and screening the probiotics compositions powder under aseptic conditions to get the finished product of probiotics compositions. The introduction of aloe juice dilution and pearl powder in the mixture preparation enables the regulation effects on the balance of intestinal flora as well as the 15 certain beauty effects, thus increasing the variety of probiotics compositions effects.

Description

ACTIVE PROBIOTICS COMPOSITIONS PREPARATION METHOD
TECHNICAL FIELD
The present invention is in the field of probiotics compositions preparation, more specifically pertaining to a method of preparing active probiotics compositions.
BACKGROUND
Active probiotic is a microorganism, divided into beneficial microorganism and harmful microorganism by the different nature and metabolism of various microorganisms, in which beneficial microorganisms is capable of enabling the oxygen free radicals (free radicals) removal, disease source elimination, cell aging delay, T cells activation, nitroso compounds and other organic carcinogenic substances decomposition, the inhibition of germs, viruses and cancer cells growth, and regulation of human blood lipid levels. The preparation of active probiotics compositions generally adds lactic acid bacteria, vitamins and proteins to the container for the well-mixed finished product. However, the current probiotics products prepared by active probiotics compositions are mostly for the balance regulation of intestinal flora, with unicity in efficacy. An active probiotics compositions preparation method is thus disclosed to solve the above-mentioned problems.
SUMMARY OF THE INVENTION
The subject invention provides a method of preparing active probiotics compositions. The introduction of aloe juice dilution and pearl powder in the mixture preparation enables the regulation effects on the balance of intestinal flora as well as the certain beauty effects, thus increasing the variety of probiotics compositions effects to solve the above-mentioned problems.
To this end, the subject invention provides following technical solution:
A method of preparing active probiotics compositions, incorporating the following procedures:
S1, cultivation and processing of lactobacillus acidophilus prepared by bovine milk, starter culture and glucose for lactobacillus acidophilus powder.
S2, cultivation and processing of lactobacillus crispatus extracted from animals for active lactobacillus crispatus powder.
S3, cultivation and processing the bovine milk inoculated with lactobacillus rhamnosus after high-temperature instant sterilization for active lactobacillus rhamnosus powder.
S4, introduction of aloe juice dilution, lactobacillus acidophilus powder, active lactobacillus crispatus powder, active lactobacillus rhamnosus powder, pearl powder and osmanthus fragrans fructooligosaccharide in turn through a sterile mixing bucket with intensive mixing for the mixture.
S5, the freeze-drying and concentration of the mixture by vacuum freeze-dryer, then being crushed by crusher to get probiotics compositions powder. 56, the filtering and screening of probiotics compositions powder under aseptic conditions, in which the small particles after screening are dispensed by storage tanks, and the storage tank is sealed and stored to get the finished product of probiotics compositions.
Preferably, the bovine milk in step 1 is fresh and high-quality whole milk or semi-skimmed milk, and the bovine milk is heated at 100°C for 1 hour before use and then immediately cooled to 37°C for sterilization operation.
Preferably, the preparation of said lactobacillus acidophilus powder begins with the addition of 1-1.5% of starter culture and 1% of glucose into sterilized bovine milk under constant temperature at 37 °C for fermentation acidity maintained at 0.6%-0.7%, and the fermentation solution cooled to below 10 °C, and then freeze-drying and concentrating the fermentation solution after stirring well and then freeze-dried and concentrated for lactobacillus acidophilus powder, and finally refrigerating the lactobacillus acidophilus powder at 5°C-6°C.
Preferably, the preparation of the lactobacillus crispatus described in step 2 begins with the extraction of lactobacillus crispatus from animal colon, then inoculating lactobacillus crispatus into the animal whole mitk for cultivation and obtaining the activated strain, followed by the freeze-drying and concentration after separation and purifying of the activated strain to obtain the active lactobacillus crispatus powder.
Preferably, the lactobacillus rhamnosus described in step 3 is extracted from cattle colon, with the extracted strain being sterilized and disinfected to obtain pure lactobacillus rhamnosus, and the bovine milk is sterilized instantaneously at high temperature by heating bovine milk at 100°C for 1 hour and then immediately cooled to 37°C for high-temperature sterilization operation.
Preferably, said aloe juice dilution in step 4 is a mixture obtained by selecting fresh aloe vera gel using distilled water for dilution, in which the distilled water used is secondary distilled water, and the ratio of fresh aloe vera gel and distilled water used is 30%-40% of fresh aloe vera gel and 60%-70% of distilled water.
Preferably, the mixing bucket is configured with a stirring assembly, said stirring assembly includes a stirring shaft and two curved stirring rods, in which said stirring shaft is rotatably connected to the inner bottom of the mixing bucket, said stirring rods are fixed to the outer wall of one end of the stirring shaft, and the other end of said stirring shaft is fitted with a drive motor through the bottom wall of the mixing bucket.
Preferably, according to weight parts, ingredients applied in said mixture are allocated as follows: 20-30 of lactobacillus acidophilus powder, 20-30 of active lactobacillus curvatus powder, 20-30 of active lactobacillus rhamnosus powder, 8-10 of pearl powder and 20-25 of osmanthus fragrans fructooligosaccharide.
Preferably, said vacuum freeze dryer in step 5 comprises a freeze-drying chamber and a cold trap, said freeze-drying chamber is installed with a partition for refrigeration, the mixture enters the freeze-drying chamber to receive vacuum treatment and the freeze-drying operation, said cold trap is applied to capture the moisture in the mixture to realize the freeze-drying of the mixture.
Preferably, the dried mixture is crushed by crusher under aseptic conditions, the large piece of mixture is crushed into powder particles with a diameter of less than 20 um. The obtained powder particles herein are the probiotics compositions powder.
Compared with the prior art, the advantages of the method of preparing active probiotics compositions in the subject invention are as follows:
This invention begins with the preparation of lactobacillus acidophilus, active lactobacillus curvatus powder and active lactobacillus rhamnosus powder respectively, then the addition of aloe juice dilution, lactobacillus acidophilus powder, active lactobacillus crispatus powder, active lactobacillus rhamnosus powder, pearl powder and osmanthus fragrans fructooligosaccharide in turn through a sterile mixing bucket with intensive mixing for the mixture, followed by the freeze-drying and concentration of the mixture by vacuum freeze-dryer, then being crushed by crusher to get probiotics compositions powder, finally filtering and screening the probiotics compositions powder under aseptic conditions, in which the small particles after screening are dispensed by storage tanks, and the storage tank is sealed and stored to get the finished product of probiotics compositions. The introduction of aloe juice dilution and pearl powder in the mixture preparation enables the regulation effects on the balance of intestinal flora as well as the certain beauty effects, thus increasing the variety of probiotics compositions effects.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
Technical solutions in embodiments of the subject invention will be clearly and completely explained in the following text. Obviously, the description of the embodiments is merely part of embodiments contained in the invention, not referring to all embodiments contained in the invention. By way of embodiments, and not limitation, embodiments of the present invention are set forth below. It is within the scope of the invention that all other embodiments obtained by ordinary personnel in the art without creative efforts according to the embodiments in the present invention.
Example 1
The present invention also provides a method of preparing active probiotics compositions, incorporating the following procedures:
S1, cultivation and processing of lactobacillus acidophilus prepared by bovine milk, starter culture and glucose for lactobacillus acidophilus powder.
The bovine milk is fresh and high-quality whole milk or semi-skimmed milk, and the bovine milk is heated at 100°C for 1 hour before use and then immediately cooled to 37°C for sterilization operation.
The preparation of lactobacillus acidophilus powder begins with the addition of 1-1.5% of starter culture and 1% of glucose into sterilized bovine milk under constant temperature at 37 °C for fermentation acidity maintained at 0.6%-0.7%, and the fermentation solution cooled to below 10 °C, and then freeze-drying and concentrating the fermentation solution after stirring well and then freeze-dried and concentrated for lactobacillus acidophilus powder, and finally refrigerating the lactobacillus acidophilus powder at 5°C-6°C. Lactobacillus acidophilus, the genus lactobacillus and a probiotic bacteria, can be applied in clinical practice to adjust the intestinal flora balance and inhibit the proliferation of undesirable microorganisms in the intestinal tract.
S2, cultivation and processing of lactobacillus crispatus extracted from animals for active lactobacillus crispatus powder.
The preparation of the lactobacillus crispatus begins with the extraction of lactobacillus crispatus from animal colon, then inoculating lactobacillys crispatus into the animal whole milk for cultivation and obtaining the activated strain, followed by the freeze-drying and concentration after separation and purifying of the activated strain to obtain the active lactobacillus crispatus powder. Lactobacillus crispatus can decompose glucose and other sugars into various bacteria of lactic acid. Being a non-budding bacillus and Gram-positive bacteria, lactobacillus crispatus is capable of maintaining human health, improving the digestion as well as absorption rate of lactose, protein and calcium, and regulating the intestinal flora.
S3, cultivation and processing the bovine milk inoculated with lactobacillus rhamnosus after high-temperature instant sterilization for active lactobacillus rhamnosus powder.
Lactobacillus rhamnosus is extracted from cattle colon, with the extracted strain being sterilized and disinfected to obtain pure lactobacillus rhamnosus, and the bovine milk is 5 sterilized instantaneously at high temperature by heating bovine milk at 100°C for 1 hour and then immediately cooled to 37°C for high-temperature sterilization operation. Lactobacillus rhamnosus, classified into the genus lactobacillus, a normal flora in the intestinal tract, enables the cholesterol attenuation, cell division promotion, intestinal flora regulation, gastrointestinal function balance and improvement, diarrhea prevention and treatment, toxins elimination, and improvement of the immunity of the body and anti-cancer effects.
S4, the addition of aloe juice dilution, lactobacillus acidophilus powder, active lactobacillus crispatus powder, active lactobacillus rhamnosus powder, then the pearl powder and osmanthus fragrans fructooligosaccharide in turn through a sterile mixing bucket with intensive mixing for the mixture.
Aloe juice dilution is selected from fresh aloe vera gel using distilled water for dilution, in which the distilled water used is secondary distilled water, and the ratio of fresh aloe vera gel and distilled water used is 30%-40% of fresh aloe vera gel and 60%-70% of distilled water; pearl powder is the raw material of pearl, gained by machine grinding and processing after sterilization, characterized by white powder with a special fishy smell, and the function of adjusting gi to regulate mind, adjusting blood to regulate mind, maintenance of liver and eyes, detoxification, beauty and skin care, etc. constituting a valuable Chinese herbal medicine; aloe vera gel contains a unique protein, capable of enabling the cell aging prevention, chronic allergies treatment, thus supplying the function of reconciling the skin and delaying skin aging.
Certain substances in aloe vera gel, mainly polysaccharides, play the role of the body's natural moisturizing factor, enabling the replenishment of the water lost in moisture and the enhancement the skin moisture.
The introduction of aloe juice dilution and pearl powder in the mixture preparation enables the regulation effects on the balance of intestinal flora as well as the certain beauty effects, thus increasing the variety of probiotics compositions effects.
The mixing bucket is configured with a stirring assembly, comprising a stirring shaft and two curved stirring rods, in which stirring shaft is rotatably connected to the inner bottom of the mixing bucket, stirring rods are fixed to the outer wall of one end of the stirring shaft, and the other end of said stirring shaft is fitted with a drive motor through the bottom wall of the mixing bucket. The stirring shaft drives the stirring rod to stir the mixture in the mixing bucket by rotating the stirring shaft as the center to enhance the mixture uniformity.
The ingredients in the mixture are applied as follows according to weight parts: 20 of lactobacillus acidophilus powder, 20 of active lactobacillus curvatus powder, 20 of active lactobacillus rhamnosus powder, 8 of pearl powder, and 20 of osmanthus fragrans fructooligosaccharide.
S5, the freeze-drying and concentration of the mixture by vacuum freeze-dryer, then being crushed by crusher to get probiotics compositions powder.
The vacuum freeze dryer comprises a freeze-drying chamber and a cold trap, in which the freeze-drying chamber is installed with a partition for refrigeration, the mixture enters the freeze-drying chamber to receive vacuum treatment and the freeze-drying operation, and the cold trap is applied to capture the moisture in the mixture to realize the freeze-drying of the mixture.
The vacuum freeze dryer includes a freeze-drying chamber and a cold trap, and the freeze-drying chamber is installed with a partition with refrigeration function, and the mixture enters the freeze-drying chamber for vacuuming before freeze-drying operation, and the cold trap is used to capture the moisture in the mixture, so that the mixture is freeze-dried.
The dried mixture is crushed by crusher under aseptic conditions, the large piece of mixture is crushed into powder particles with a diameter of less than 20 um. The obtained powder particles herein are the probiotics compositions powder.
S6, the filtering and screening of probiotics compositions powder under aseptic conditions, in which the small particles after screening are dispensed by storage tanks, and the storage tank is sealed and stored to get the finished product of probiotics compositions. The large particles of the screened powder will be crushed twice to facilitate the recycling of the large particles of the powder, in which the small particles of the powder are the screened powder with a diameter less than 20um, while the large particles of the powder are greater than or equal to 20um in diameter.
Example 2
The similarities won't go into much detail here, unlike example 1, the ingredients in the mixture of Step 4 are applied as follows according to weight parts: 25 of lactobacillus acidophilus powder, 25 of active lactobacillus curvatus powder, 25 of active lactobacillus rhamnosus powder, 9 of pearl powder, and 23 of osmanthus fragrans fructooligosaccharide.
Example 3
The similarities won't go into much detail here, unlike example 1 and example 2, the ingredients in the mixture are applied as follows according to weight parts: 30 of lactobacillus acidophilus powder, 30 of active lactobacillus curvatus powder, 30 of active lactobacillus rhamnosus powder, 10 of pearl powder, and 25 of osmanthus fragrans fructooligosaccharide.
In conclusion, the foregoing preparation method of probiotics compositions contains the ingredients of lactobacillus acidophilus, active lactobacillus curvatus, active lactobacilius rhamnosus, aloe juice dilution and pearl powder, enabling the regulation effects on the balance of intestinal flora as well as the certain beauty effects, thus increasing the variety of probiotics compositions effects.
Finally, it should be stated that the foregoing descriptions of the embodiments of the invention are intended to illustrate the preferred embodiments and not to limit this invention.
Despite the invention described in detail with reference to the foregoing embodiments, for personnel in the art, modifications may be made on the technical solutions recorded in the foregoing embodiments, or alternations may be made on part of the technical features. The present disclosure may take on any modifications, alterations, and improvements without departing from the spirit and scope of the disclosure.

Claims (10)

CONCLUSIECONCLUSION 1. Methode voor de bereiding van een actieve probiotische samenstelling, met het kenmerk dat zij de volgende stappen bevat:1. Method for the preparation of an active probiotic composition, characterized in that it comprises the following steps: S1. Kweken en verwerken van Lactobacillus acidophilus bereid met rauwe melk, starter en glucose om een Lactobacillus acidophilus poeder te verkrijgen;S1. Cultivation and processing of Lactobacillus acidophilus prepared with raw milk, starter and glucose to obtain a Lactobacillus acidophilus powder; S2. Kweken en verwerken van Lactobacillus curlicus geëxtraheerd van dieren om actief lactobacillus curlicus poeder te verkrijgen;S2. Cultivation and processing of Lactobacillus curlicus extracted from animals to obtain active Lactobacillus curlicus powder; S3. Melkgrondstoffen enten met Lactobacillus rhamnosus na onmiddellijke sterilisatie bij hoge temperatuur, en vervolgens kweken en verwerken om actief Lactobacillus rhamnosus-poeder te verkrijgen;S3. Inoculating milk raw materials with Lactobacillus rhamnosus after instant high temperature sterilization, then culturing and processing to obtain active Lactobacillus rhamnosus powder; S4. Toevoegen van aloë sap verdunningsmiddel in een steriel mengvat, toevoegen van Lactobacillus acidophilus poeder, actieve lactobacillus curlicus poeder en actieve Lactobacillus rhamnosus poeder en mengen ervan in het mengvat, dan parel poeder en osmanthus oligofructose toevoegen en blijven roeren, en volledig mengen om het mengsel te verkrijgen;S4. Add aloe juice diluent into a sterile mixing vessel, add Lactobacillus acidophilus powder, active Lactobacillus curlicus powder and active Lactobacillus rhamnosus powder and mix them into the mixing vessel, then add pearl powder and osmanthus oligofructose and keep stirring, and mix completely to make the mixture to acquire; S5. Vriesdrogen en concentreren van het mengsel door een vacuüm vriesdroger, en dan verpletteren met een breker om probiotische samenstelling poeder te verkrijgen;S5. Freeze-drying and concentrating the mixture by a vacuum freeze dryer, and then crushing with a crusher to obtain probiotic composition powder; S6. Het poeder van de probiotische samenstelling onder steriele omstandigheden filteren en screnen, onderbrengen van het gefilterde poeder met kleine deeltjes in een opslagtank die daarna wordt verzegeld en opgeslagen om de afgewerkte probiotische samenstelling te verkrijgen.S6. Filtering and screening the powder of the probiotic composition under sterile conditions, placing the filtered powder with small particles in a holding tank which is then sealed and stored to obtain the finished probiotic composition. 2. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 1, met het kenmerk dat in stap 1 voor de rauwe melk gebruik wordt gemaakt van verse volle melk van hoge kwaliteit of halfvolle melk, en de rauwe melk vóór gebruik gedurende 1 uur wordt verwarmd bij 100 °C, en vervolgens onmiddellijk wordt afgekoeld tot 37 °C om te worden gesteriliseerd,Method for the preparation of an active probiotic composition according to claim 1, characterized in that in step 1 for the raw milk use is made of high-quality fresh whole milk or semi-skimmed milk, and the raw milk is cooled for 1 hour before use. heated at 100 °C, and then immediately cooled to 37 °C to be sterilized, 3. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 2, met het kenmerk dat tijdens de voorbereiding van het Lactobacillus acidophilus poeder de gesteriliseerde rauwe melk wordt toegevoegd met 1-1.5% starter en 1% glucose, en de thermostatische gisting wordt uitgevoerd bij 37 °C, zodat de zuurheid na gisting 0 is, waarbij 6%-0.7% van de vergiste bouillon wordt gekoeld aan een temperatuur onder 10 °C, en daarna wordt de vergiste bouillon gelijkmatig geroerd, gevriesdroogd en geconcentreerd om Lactobacillus acidophiluspoeder te verkrijgen, tot slot wordt het Lactobacillus acidophiluspoeder gekoeld bij 5 °C -6 °C,Method for the preparation of an active probiotic composition according to claim 2, characterized in that during the preparation of the Lactobacillus acidophilus powder, the sterilized raw milk is added with 1-1.5% starter and 1% glucose, and the thermostatic fermentation is carried out at 37°C, so that the acidity after fermentation is 0, cooling 6%-0.7% of the fermented broth at a temperature below 10°C, and then the fermented broth is evenly stirred, freeze-dried and concentrated to obtain Lactobacillus acidophilus powder , finally, the Lactobacillus acidophilus powder is refrigerated at 5 °C -6 °C, 4. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 1, met het kenmerk dat tijdens de bereiding van het gekrulde Lactobacillus-poeder in stap 2, de gekrulde Lactobacillus eerst uit de dikke darm van het dier wordt geëxtraheerd, en dan in de dierlijke volle melk voor cultuur wordt geënt om de geactiveerde bacteriën te verkrijgen, en dan de geactiveerde bacteriën worden gescheiden en gezuiverd, gevriesdroogd en geconcentreerd om het actieve gekrulde Lactobacillus-poeder te verkrijgen.Method for the preparation of an active probiotic composition according to claim 1, characterized in that during the preparation of the curled Lactobacillus powder in step 2, the curled Lactobacillus is first extracted from the colon of the animal, and then in the animal whole milk for culture is inoculated to obtain the activated bacteria, and then the activated bacteria are separated and purified, freeze-dried and concentrated to obtain the active curly Lactobacillus powder. 5. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 1, met het kenmerk dat in stap 3, de Lactobacillus rhamnosus een stam is die geëxtraheerd is uit de dikke darm van runderen, waarbij de geëxtraheerde stam wordt gesteriliseerd en gedesinfecteerd om zuivere Lactobacillus rhamnosus te verkrijgen, waarbij de methode van onmiddellijke sterilisatie van melk bij hoge temperatuur erin bestaat om de melk te verhitten tot 100 °C en dan onmiddellijk af te koelen tot 37 °C, en de melk bij hoge temperatuur te steriliseren.Method for the preparation of an active probiotic composition according to claim 1, characterized in that in step 3, the Lactobacillus rhamnosus is a strain extracted from the large intestine of cattle, the extracted strain being sterilized and disinfected to obtain pure Lactobacillus rhamnosus, where the method of instant sterilization of milk at high temperature is to heat the milk to 100 °C and then immediately cool it to 37 °C, and sterilize the milk at high temperature. 6. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 1, met het kenmerk dat in stap 4, het aloé sap verdunningsmiddel een mengsel is verkregen door het selecteren van verse aloë gel en verdunnen met gedestilleerd water, het gedestilleerde water secundair gedestilleerd water is, en de verhouding van het gebruik van verse aloé gel en gedestilleerd water 30%-40% is van verse aloë gel en 60%-70% van gedestilleerd water.Method for the preparation of an active probiotic composition according to claim 1, characterized in that in step 4, the aloe juice diluent is a mixture obtained by selecting fresh aloe gel and diluting with distilled water, the distilled water being secondary distilled water is, and the ratio of using fresh aloe gel and distilled water is 30%-40% of fresh aloe gel and 60%-70% of distilled water. 7. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 6, met het kenmerk dat een roerdercomponent in het mengvat geïnstalleerd is, de roerdercomponent een roerderas en twee boogvormige roerstaven omvat, de roerderas roterend verbonden is met de binnenbodem van het mengvat, de roerderstaaf bevestigd is aan de buitenwand van één uiteinde van de roerderas, en het andere uiteinde van de roerderas geïnstalleerd is met een aandrijfmotor doorheen de bodemwand van het mengvat.Method for the preparation of an active probiotic composition according to claim 6, characterized in that a stirrer component is installed in the mixing vessel, the stirrer component comprises a stirrer shaft and two arcuate stirrers, the stirrer shaft is rotatably connected to the inner bottom of the mixing vessel, the stirrer rod is attached to the outer wall of one end of the stirrer shaft, and the other end of the stirrer shaft is installed with a driving motor through the bottom wall of the mixing vessel. 8. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 7, met het kenmerk dat volgens de massacomponenten, de grondstofverhouding die in het mengsel wordt gebruikt is: 20-30 delen van Lactobacillus acidophilus poeder, 20-30 delen van actief lactobacillus crimpus poeder, 20-30 delen van actief Lactobacillus rhamnosus poeder, 8-10 delen van parelpoeder, en 20-25 delen van Osmanthus oligofructose.A method for preparing an active probiotic composition according to claim 7, characterized in that according to the mass components, the raw material ratio used in the mixture is: 20-30 parts of Lactobacillus acidophilus powder, 20-30 parts of active Lactobacillus crimpus powder, 20-30 parts of active Lactobacillus rhamnosus powder, 8-10 parts of pearl powder, and 20-25 parts of Osmanthus oligofructose. 9. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 1, met het kenmerk dat in stap 5, de vacuümvriesdroger bestaat uit een vriesdroogkamer en een koelval, de vriesdroogkamer is uitgerust met een tussenschot met een koelfunctie, het mengsel de vriesdroogkamer binnengaat en wordt vacuüm wordt getrokken alvorens te vriesdrogen, en de koelval wordt gebruikt om het vocht in het mengsel op te vangen, zodat het mengsel wordt gevriesdroogd.A method for the preparation of an active probiotic composition according to claim 1, characterized in that in step 5, the vacuum freeze-dryer consists of a freeze-drying chamber and a cooling trap, the freeze-drying chamber is equipped with a partition with a cooling function, the mixture enters the freeze-drying chamber and vacuum is drawn before freeze-drying, and the cold trap is used to collect the moisture in the mixture, so that the mixture is freeze-dried. 10. Methode voor de bereiding van een actieve probiotische samenstelling volgens conclusie 9, met het kenmerk dat de breker het gedroogde mengsel onder steriele omstandigheden breekt, grote stukken van het mengsel breekt tot poederdeeltjes met een diameter van minder dan 20um, en de verkregen poederdeeltjes probiotisch samenstellingspoeder zijn.Method for the preparation of an active probiotic composition according to claim 9, characterized in that the crusher breaks the dried mixture under sterile conditions, breaks large pieces of the mixture into powder particles with a diameter of less than 20 µm, and the resulting powder particles are probiotically compound powder.
NL2032234A 2022-06-21 2022-06-21 Active probiotics compositions preparation method NL2032234B1 (en)

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