CN1057296A - A kind of method of producing lactobacillin - Google Patents
A kind of method of producing lactobacillin Download PDFInfo
- Publication number
- CN1057296A CN1057296A CN 91100573 CN91100573A CN1057296A CN 1057296 A CN1057296 A CN 1057296A CN 91100573 CN91100573 CN 91100573 CN 91100573 A CN91100573 A CN 91100573A CN 1057296 A CN1057296 A CN 1057296A
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- milk
- lactobacillin
- substratum
- fermentation
- bacterial classification
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Abstract
Lactobacillin is a Lactobacterium acidophilum, a kind of antibiotics of excretory in the cow's milk substratum, the synthesis of thalline and substratum.Has digestion-aid effect.Be used for abnormal fermentation in the intestines, maldigestion, enteritis and diseases such as infantile diarrhea and malnutrition.
(Lactibaci llus acidophi lous) is bacterial classification with Lactobacterium acidophilum, is inoculated in the skimmed milk, through fermentation, dry and make oyster white or micro-yellow powder.
Description
What the present invention relates to is the processing method that is prepared the lactic acid bacteria class goods by Mammals milk, particularly be the processing method for preparing the lactobacillin medicine by cow's milk.
Before a century, the scientist Mei Qinikefu of Russia finds, Caucasia and Bulgarian people's life-span are long, be because a kind of sour milk of this area's people's informal dress (solidifying breast), wherein contain lactobacillus (bacillus bulgaricus), Mei Shi thought at that time: lactobacillus produces acid in enteron aisle, suppressed the spoilage organism growth, thereby bacterial bacillary dysentery, colitis in having cured, just disease such as secretes.
Have only lactasinum at Chinese lactic acid bacteria formulation, said preparation is the streptococcus acidi lactici preparation of living, and is to be the active bacteria formulation that thinner is made with starch with the work streptococcus acidi lactici.These product can not be taken simultaneously with anti-inflammation drugs such as sulfamido, microbiotic, can not use with compatibilities such as bismuth subcarbonate, tannin albuminate, carbo medicinalises.These product are taken also for a long time can cause subacute endocarditis.The lactasinum pharmacological action is.Streptococcus acidi lactici produces lactic acid and suppresses intestinal bacterium after the enteron aisle internal breeding, play abnormal fermentation effect in the control intestines, but, when suffering from gastrointestinal dysfunction, hyperfunction with intestinal peristalsis often, diarrhoea, to get rid of externally less than the gastrointestinal contents of digestion rapidly, the viable bacteria in the lactasinum does not also grow surely in enteron aisle and promptly is excluded externally, just can't bring into play its curative effect yet.Therefore be necessary to develop new similar drug to overcome above-mentioned defective.
Primary and foremost purpose of the present invention provide a kind of aid digestion, can treat illnesss such as enteritis, maldigestion, do not have the short lactobacillin similar drug that falls into of above-mentioned prior art medicine simultaneously.
Further aim of the present invention provides a kind of method for preparing the lactobacillin medicine.
The inventor is on the basis of summing up the lactasinum pharmacological mechanism, selecting Lactobacterium acidophilum for use is bacterial classification, with defatted mammalian milk juice is substratum, and biology has synthesized and can participate in organism metabolism, contains lactic acid with physiologically active and suppress dysentery bacterium, colibacillary antibiotic medicine-lactobacillin outside human body.
After the preparation method of described lactobacillin medicine comprises the steps: Mammals milk carried out degreasing, with it is substratum, the inoculating lactobacillus acidophilus, under 20～55 ℃ temperature, ferment, when reaching 160～350 ° of T, fermented liquid acidity stops fermentation, the fermented liquid that finally obtains promptly is a lactobacillin solution, it be the Lactobacterium acidophilum thalline and in substratum the mixture of excretory antibiotics and substratum.
The used raw material Mammals milk of method of the present invention comprises cow's milk, sheep breast etc., and preferably fresh milk particularly preferably is fresh cow milk.
Method of the present invention preferably after the milk degreasing, concentrates earlier and inoculates described bacterial classification then, and more preferably milk being concentrated into degree Beaume is 9～11.
The used bacterial classification of method of the present invention is Lactobacterium acidophilum (Lactobacillus acidonhilns), it commercial be available.Preferred bacterial classification is the third generation bacterial classification of enlarged culturing.The process of enlarged culturing is, the commercial bacterial classification that obtains is carried out purifying, removes the impurity elimination bacterium, obtains pure Lactobacterium acidophilum, is substratum with degreasing milk, obtains first-generation bacterial classification through breeding, again through breeding, obtains bacterial classification for the second time, obtains third generation bacterial classification through breeding again; Then third generation bacterial classification is carried out vitality test, the mensuration process is: add 3ml third generation bacterial classification in 100ml degreasing milk, under 42 ℃ of temperature condition, cultivated 7～10 hours, produce lactic acid production 110～140 ° of T(gill Nie Er acidity) time for normal (vigor is arranged) bacterial classification, can be used for inoculation.
Postvaccinal substratum can ferment under 20～55 ℃ temperature, also can ferment in this extraneous proper temperature scope, and this can be very clear to those skilled in the art, and preferred leavening temperature is 38～50 ℃.
When fermented liquid acidity reaches 160～350 ° of T(gill Nie Er acidity) time can stop the fermentation, preferably when fermented liquid acidity reaches 270～320 ° of T, stop the fermentation, the fermented liquid that at this moment obtains promptly is the lactobacillin aqueous solution.
Lactobacillin of the present invention can be multiple formulation, as the aqueous solution, powder agent tablet etc.Make the powder agent tablet as need, the fermented liquid after the fermentation ends can be carried out drying and dust, obtain granular substance, make the lactobacillin powder after pulverizing then, sieving, again through well known to a person skilled in the art that film-making technology can obtain tablet.
Below by embodiment method of the present invention is described further.
Embodiment 1
Get the following processing step of fresh milk process:
1. test breast: measure its proportion, acidity, and measure its fat content with alcohol test.
2. degreasing: cold storage cow's milk through giving hot device, giving heat to 28～30 ℃, is pumped into milk clarifier and separating machine with milk pump, pour cream into storage tanker, pour skimming milk into milk tank, with 10～15 ℃ of cold water circulation cooling preservation.
3. concentrate: with being pumped in the sterilization vapour, sterilization ℃ is carried out in steam-heated cal(l)andria to 86～92, pours into then and concentrates the economic benefits and social benefits thin-film evaporator with skimmed milk, and this evaporator operation is preceding with being steam heated to 86～92 ℃ of sterilizations, 0.5～1.5 kilogram/cm of vapor pressure
2, vacuum tightness 700～720 mmhg were sterilized after 0.5～1 minute, poured the sterilization skimming milk into, and logical steam heats, and temperature is controlled at 60 ℃, and vacuum tightness is not less than 600 mmhg, and emulsion is concentrated into 9～11 degree Beaume.After concentrating, emulsion is poured in the fermentor tank.
4. fermentation: with the fermentor tank cleaning and with after the sterilization of steam detoxicating, import concentrated emulsion, make emulsion humidity reduce to 43～46 ℃ by water cooler, the meter capacity is got third generation lactobacterium acidophilus kind by 3～5% of emulsion weight and is inoculated, and is incubated 18～24 hours.After beginning to ferment 4 hours, per hour survey acidity once, finish fermentation when above when acidity reaches 270～320 ° of T.
5. drying is dusted: fermented liquid is made granulated powders through general spray drying device, and the powder injection process condition is: 130～145 ℃ of inlet temperatures, row mouthful 62～65 ℃ of vapor pressure 4.5～6.5Kg/cm of temperature
2, relative temperature 10～14%.
6. sieve powder: with ultra-violet sterilization 15 minutes, pulverizer disinfected in alcohol before work in sieve powder chamber, and the particle that obtains in will be 5. with pulverizer is crushed to 60 orders, with the lactobacillin powder that the obtains plastics bag of packing into.
Lactobacillin of the present invention contains amino acid (seeing Table I) and a large amount of vitamin B group and potassium, sodium, calcium, magnesium, iron, zinc, selenium and other trace elements (seeing Table II).Lactobacillin is not subjected to the influence of the first-pass effect of hydrochloric acid in gastric juice, enters behind the small intestine to reduce the enteron aisle pH value, and the spoilage organism that makes some produce alkali is suppressed, and abnormal fermentation must be controlled in the intestines, thereby the restoration of gastrointestinal function of disorder is normal.Lactic acid in the lactobacillin can reduce the burden of stomach, can also promote stomach and intestine normal creepage of gastrointestinal functions and various secretion of digestive juice (the sick observation of curative effect of 150 routine enteron aisles sees Table III).Anti-inflammation drugs such as lactobacillin and sulfanilamide (SN), microbiotic do not have incompatibility, compare with antiphlogistic drugs such as the berberine for the treatment of enteritis, paraxin, terramycin, Trichofurons, without any side effects such as toxic reactions, lactobacillin and said medicine associating Wu Yongke play synergy.Lactobacillin does not have validity period, and this point is different with lactasinum.The performance comparison of the medicine of lactobacillin and prior art (seeing Table IV).
Claims (12)
1, a kind of method of producing lactobacillin, it is characterized in that this method may further comprise the steps: Mammals milk is carried out degreasing, is substratum, inoculating lactobacillus acidophilus then with it, keep 20～55 ℃ temperature to ferment, when fermented liquid acidity reaches 160～350T, stop fermentation.
2, the method for claim 1 is characterized in that described animal milk is a cow's milk.
3, method as claimed in claim 1 or 2 is characterized in that concentrating after the described milk degreasing, then the inoculating lactobacillus acidophilus.
4, method as claimed in claim 3 is characterized in that it is 9～11 that described degreasing milk is concentrated into degree Beaume.
5,, it is characterized in that described Lactobacterium acidophilum is the third generation bacterial classification through enlarged culturing as claim 1 or 2 or 4 described methods.
6, method as claimed in claim 5, the addition that it is characterized in that described bacterial classification are 1～7%(weight of substratum).
7, method as claimed in claim 6, the addition that it is characterized in that bacterial classification are 3～5%(weight of substratum).
8,, it is characterized in that fermentation time is 18～24 hours as claim 1 or 2 or 4 described methods.
9, method as claimed in claim 1 or 2 is characterized in that described milk is fresh milk.
10, as claim 1 or 2 or 6 described methods, it is characterized in that after fermentation ends, dusting.
11, method as claimed in claim 1 or 2 is characterized in that fermenting under 38～50 ℃ temperature.
12, method as claimed in claim 1 or 2 is characterized in that stopping fermentation when fermented liquid acidity reaches 270～320 ° of T.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91100573 CN1057296A (en) | 1991-02-02 | 1991-02-02 | A kind of method of producing lactobacillin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91100573 CN1057296A (en) | 1991-02-02 | 1991-02-02 | A kind of method of producing lactobacillin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1057296A true CN1057296A (en) | 1991-12-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91100573 Pending CN1057296A (en) | 1991-02-02 | 1991-02-02 | A kind of method of producing lactobacillin |
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| CN (1) | CN1057296A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103249830A (en) * | 2010-12-10 | 2013-08-14 | 帝斯曼知识产权资产管理有限公司 | Starter culture compositions |
| CN103451134A (en) * | 2013-09-06 | 2013-12-18 | 江南大学 | Lactobacillus hilgardii and application of lactobacillus hilgardii in yellow rice wine brewing |
| CN106901344A (en) * | 2017-03-16 | 2017-06-30 | 北京知蜂堂健康科技股份有限公司 | A kind of composition for alleviating stomachache stomachache and its application |
| CN107723233A (en) * | 2017-11-23 | 2018-02-23 | 呼伦贝尔康益药业有限公司 | A kind of apparatus and method for producing lactein |
| CN108103128A (en) * | 2017-12-01 | 2018-06-01 | 安佑生物科技集团股份有限公司 | A kind of preparation method for the lactein for being used to treat grice diarrhoea |
| CN109717291A (en) * | 2019-03-18 | 2019-05-07 | 马亚宾 | A kind of production technology of the lactein suitable for intestinal health |
-
1991
- 1991-02-02 CN CN 91100573 patent/CN1057296A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103249830A (en) * | 2010-12-10 | 2013-08-14 | 帝斯曼知识产权资产管理有限公司 | Starter culture compositions |
| CN103451134A (en) * | 2013-09-06 | 2013-12-18 | 江南大学 | Lactobacillus hilgardii and application of lactobacillus hilgardii in yellow rice wine brewing |
| CN103451134B (en) * | 2013-09-06 | 2015-05-13 | 江南大学 | Lactobacillus hilgardii and application of lactobacillus hilgardii in yellow rice wine brewing |
| CN106901344A (en) * | 2017-03-16 | 2017-06-30 | 北京知蜂堂健康科技股份有限公司 | A kind of composition for alleviating stomachache stomachache and its application |
| CN107723233A (en) * | 2017-11-23 | 2018-02-23 | 呼伦贝尔康益药业有限公司 | A kind of apparatus and method for producing lactein |
| CN108103128A (en) * | 2017-12-01 | 2018-06-01 | 安佑生物科技集团股份有限公司 | A kind of preparation method for the lactein for being used to treat grice diarrhoea |
| CN109717291A (en) * | 2019-03-18 | 2019-05-07 | 马亚宾 | A kind of production technology of the lactein suitable for intestinal health |
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