JP5855375B2 - Anti-fatigue composition - Google Patents

Description

  The present invention relates to an anti-fatigue composition. More specifically, the present invention relates to an anti-fatigue composition containing, as an active ingredient, a fermented composition obtained by inoculating Bacillus natto into a medium containing rice bran and soybeans and fermenting them.

  Conventionally, in aerobic exercise such as sports and labor, compositions and the like having a fatigue recovery effect that suppress a decrease in exercise performance resulting from muscle fatigue have been developed. For example, the following Patent Document 1 discloses a composition for inhibiting muscle damage, characterized by containing cacao mass and / or a processed product thereof as an active ingredient. Patent Document 2 below discloses a health food or drink characterized by containing a peptide (soybean peptide), maca extract, and ginger or ginger extract.

  One of the causes of muscle fatigue is the accumulation of fatigue substances. This fatigue substance is a metabolic substance when muscles are active, and lactic acid is known as a representative one. Muscles contract by breaking down glycogen (sugar), and lactic acid is also produced during the degradation process. The produced lactic acid is carried out by the blood and reused as energy by the function of the liver, but some lactic acid stays in the muscle, and the remaining lactic acid oxidizes the muscle and inhibits muscle activity to the body. It is thought to cause muscle fatigue. Therefore, compositions that exhibit a fatigue suppression effect or a fatigue recovery effect by suppressing lactic acid production and lactic acid accumulation have been developed. For example, Patent Document 3 listed below has an anti-endurance effect and an anti-lactic acid production-suppressing effect in exercises containing glycogen having a molecular weight of 3000 to 9500, which can be produced by heating and pressurizing a saccharide raw material under acidic conditions. A fatigue food or drink is disclosed.

JP 2006-282573 A JP 2007-222116 A JP 2005-213185 A

However, the muscle damage-suppressing compositions, health foods and drinks and anti-fatigue foods and drinks described in Patent Documents 1 to 3 are still not sufficient in anti-fatigue effects.
An object of the present invention is to provide an anti-fatigue composition which has an improvement in endurance and an anti-fatigue effect, and is excellent in suppressing a decrease in exercise performance (exercise ability).

The present invention is as follows.
1. An anti-fatigue composition characterized by using a fermented composition obtained by inoculating natto bacteria on a medium containing rice bran, soybeans, phytic acid and / or a salt thereof, and arginine and fermenting and culturing the same. Manufacturing method .
2. The content of the soybeans, the phytic acid and / or salt thereof, and the arginine in the medium is 50 to 200 parts by mass with respect to 100 parts by mass of the rice bran, respectively. A method for producing an anti-fatigue composition as described in 1. above.

The anti-fatigue composition of the present invention comprises a fermented composition obtained by inoculating natto bacteria in a medium containing rice bran and soybeans and fermented and cultured as an active ingredient, thereby inhibiting lactic acid production or blood. Since it is excellent in the lactic acid accumulation inhibitory effect, it can be set as the anti-fatigue composition which is excellent in the endurance improvement and the anti-fatigue effect, and has the effect of suppressing the fall of exercise performance.
In addition, when it is a lactic acid production inhibitor and / or a blood lactic acid accumulation inhibitor, it has a lactic acid production inhibitory action and / or a blood lactic acid accumulation inhibitory action, and has an excellent exercise performance reduction inhibitory effect and anti-fatigue effect. An anti-fatigue composition can be provided.

It is a figure which shows the elapsed time change of the momentum value (power value (W) shown by an ergometer) in the bicycle ergometer exercise test at the time of ingestion about the test article A and the control article B which are anti-fatigue compositions of the present invention. is there. It is a figure which shows the change of the lactic acid level in the blood before and behind the bicycle ergometer exercise test at the time of ingestion about the test product A and the control product B which are anti-fatigue compositions of this invention.

The method for producing an anti-fatigue composition of the present invention comprises a fermented composition obtained by inoculating natto bacteria in a medium containing rice bran, soybeans, phytic acid and / or a salt thereof, and arginine, followed by fermentation and culture. It is characterized by using a thing.

The above-mentioned fermented composition, and rice bran such, inoculated and soybeans, and phytic acid and / or salts thereof, Bacillus natto in medium containing arginine, a composition obtained by fermentation. As said fermentation composition, the liquid which filtered the culture (fermented liquid) obtained by culture | cultivation may be sufficient, and the liquid which carried out post-processing of this, such as decoloring, may be sufficient. Furthermore, the concentrate which concentrated these may be sufficient. In addition, a solid or powdered powder from which the solvent has been removed by a known method such as freeze drying or spray drying may be used.

The medium in the present invention, the rice bran such, and soybeans, phytic acid and / or its salt, and arginine are added as needed, which include other carbon Minamoto no Hitoshi, natto (microorganism described later ) Is not particularly limited as long as it can grow. Usually, it is a liquid medium, but may be a solid medium.

The rice bran means one containing at least a part of pericarp, seed coat, germ and the like produced when white rice is polished (polished rice). Examples of the rice bran include rice bran, rice germ, defatted rice germ, defatted rice bran, and rice bran extract. These can be used alone or in combination of two or more.
The soybeans mean seeds obtained from soybean (Glycine max), which is an annual plant of the leguminous family, and the presence or absence of processing is not particularly limited. Examples of the soybeans include raw soybeans, dried soybeans, defatted soybeans, quina flour, suda, soybean flour, soybean meal, soybean extract, soybean protein, soybean peptide, and hydrolysates thereof. These can use 1 type (s) or 2 or more types.

  As a mixing ratio of rice bran and soybeans in the medium, when rice bran is 100 parts by mass, soybeans are preferably 10 to 1000 parts by mass, more preferably 20 to 500 parts by mass, and still more preferably. Is 50 to 200 parts by mass. Moreover, as the amount of rice bran and soybeans added to the medium, when the total amount of the medium is 100% by mass, both rice bran and soybeans are preferably 0.1 to 15% by mass, respectively. Preferably it is 0.5-5 mass%. When it is in these ranges, the bacteria can grow well.

Moreover, as said culture medium, carbon sources other than rice bran and soybeans (henceforth only "carbon source") can be further contained as needed. As this carbon source, what is normally used for fermentation etc. can be used, For example, glucose, dextrin, lactose, starch, etc. are mentioned. These can use 1 type (s) or 2 or more types.
As a compounding quantity when a carbon source is contained in a culture medium, when rice bran is 100 parts by mass, it is preferably 10 to 1000 parts by mass, more preferably 20 to 500 parts by mass, and further preferably 50 to 50 parts by mass. 200 parts by mass. Moreover, as a compounding quantity to the culture medium of a carbon source, when a culture medium whole quantity is 100 mass%, Preferably it is 0.1-15 mass%, More preferably, it is 0.5-5 mass%. When it is in these ranges, the bacteria can grow well.

The upper SL medium, at least one of phytic acid and salts thereof (hereinafter, simply referred to as "phytic acid".) It contains.
The phytic acid may be synthetic phytic acid or extracted and isolated phytic acid. Further, as the salt thereof, a non-toxic salt is used, and a salt with a metal, an organic base, a basic amino acid, an organic ester residue or the like is used. For example, potassium, sodium, arginine, ornithine, lysine, histidine, glucamine Or a salt such as monoethanolamine.
The amount of phytic acid to be contained in the medium is preferably 10 to 1000 parts by mass, more preferably 20 to 500 parts by mass, and still more preferably 50 to 100 parts by mass when the rice bran is 100 parts by mass. 200 parts by mass. Moreover, as a compounding quantity of the phytic acid to the culture medium, when the total amount of the culture medium is 100% by mass, it is preferably 0.1 to 15% by mass, and more preferably 0.5 to 5% by mass. When it exists in these ranges, growth of a microbe is favorable, and also the obtained fermentation composition shall be excellent in the exercise performance fall inhibitory effect.

Further, media in the present invention, a rice bran acids, and soybeans, and phytic acid and / or salt thereof, containing arginine, further normally also contain other additives used in the cultivation of the bacterium it can. Examples of other additives include amino acids, vitamins, pH adjusters, and antifoaming agents. These may be used alone or in appropriate combination of two or more.

Examples of the amino acids include arginine, glycine, glutamine and glutamic acid. These amino acids may be used alone or in combination of two or more. Of these, the media in the present invention, you at least arginine.
As content in the case of containing amino acids, when rice bran is made into 100 mass parts, it becomes like this. Preferably it is 10-1000 mass parts, More preferably, it is 20-500 mass parts, More preferably, it is 50-200 masses. Part. The amount of amino acids to be added to the medium is preferably 0.1 to 15% by mass, more preferably 0.5 to 5% by mass, when the total amount of the medium is 100% by mass.

Examples of the vitamins include vitamin B1, vitamin B6, vitamin B12, nicotinic acid, nicotinic acid amide, inositol, myo-inositol, vitamin K, and the like. These vitamins may use only 1 type and may use 2 or more types together.
As content in the case of containing the said vitamin acids, when rice bran is made into 100 mass parts, Preferably it is 0.1-50 mass parts, More preferably, it is 1-10 mass parts.

Examples of the pH adjusting agent include bases (alkalizing agents) such as potassium hydroxide, sodium hydroxide, potassium carbonate, and sodium bicarbonate, and acids (oxidation) such as citric acid, gluconic acid, succinic acid, lactic acid, and carbon dioxide. Agent). These pH adjusters may use only 1 type and may use 2 or more types together.
The addition amount of the pH adjusting agent is appropriately selected such that the desired pH is obtained in the medium preparation step and the fermentation culture step.

  Moreover, as said antifoamer, the antifoamer normally used for the culture medium in culture | cultivation can be used. For example, a silicone type antifoamer, a polyether type antifoamer, etc. are mentioned. These antifoaming agents may use only 1 type and may use 2 or more types together. The blending amount of the antifoaming agent in the medium is preferably 0.01 to 1% by mass, more preferably 0.05 to 0.5% by mass when the total amount of the medium is 100% by mass.

  As a microorganism for obtaining the fermentation composition, Bacillus subtilis var. Natto is used. The fermented composition according to the present invention may be a natural or chemical mutant such as nitrosoguanidine, an X-ray, ultraviolet light, or the like obtained by an artificial mutation means, and a mutant strain having mycological properties mutated. It can be used as long as it does not lose its property of producing. Moreover, these natto bacteria may use together 1 type and 2 or more types, Furthermore, in addition to the said natto bacteria, you may use together 1 type and 2 or more types of another bacteria and yeast.

There is no restriction | limiting in particular about the fermentation culture conditions in the fermentation culture process for obtaining the said fermentation composition, as long as fermentation is performed. Usually, fermentation culture is performed by aeration and stirring, and the culture temperature is preferably about 30 to 45 ° C, more preferably 35 to 40 ° C.
The pH is preferably 6.5 to 10, more preferably 7.0 to 9.0, and still more preferably 7.5 to 8.5. When adjusting the pH of the medium, the above alkalizing agent (preferably potassium hydroxide or the like) can be used.
Moreover, although culture | cultivation time is suitably selected by various conditions, Preferably it is 6 to 48 hours, More preferably, it is 12 to 24 hours, More preferably, it is 15 to 20 hours.

  In the fermentation culture, the medium is preferably sterilized at 90 to 130 ° C. for 10 to 30 minutes and then cooled. Next, natto bacteria are inoculated, and aeration and agitation are performed within the culture temperature range for the culture time to perform fermentation culture. When the fermentation is terminated after reaching the culture time, the above-mentioned oxidizing agent (preferably citric acid or the like) is added to lower the pH (for example, the pH value is 6 or less, and more Preferably, it is 5 or less, more preferably 4 or less, and usually 1 or more.) Thus, fermentation can be terminated and a culture (fermented liquid) can be obtained. The obtained culture is subjected to solid-liquid separation by centrifugation or the like, if necessary, and then the separated liquid is filtered with a filter paper or the like, whereby the filtrate can be obtained as a fermentation composition.

  The fermentation composition in the present invention may be a fermentation broth obtained by culturing a liquid medium, or may be a filtered liquid. Further, it may be a liquid after post-treatment such as decoloring, or a concentrated liquid obtained by concentrating them. In addition, when the fermentation composition in the present invention is a liquid such as the fermentation liquid, filtrate or concentrated liquid shown above, the solid content concentration is not particularly limited, but for example, 0.1 to 50% by mass It can also be 1-20 mass%.

  The anti-fatigue composition of this invention contains said fermentation composition as an active ingredient. Content of a fermentation composition is not specifically limited, It may contain as a part of anti-fatigue composition, and may comprise the whole quantity of an anti-fatigue composition. For example, as content of the fermentation composition in an anti-fatigue composition, when the total amount of an anti-fatigue composition is 100 mass%, it is 0.0001-50 by solid content (solid content conversion amount) of a fermentation composition. It can be set to mass%, or can be 0.001 to 20 mass%.

  The anti-fatigue in the anti-fatigue composition of the present invention means that it has an effect of improving body endurance in exercise and the like and suppressing body fatigue due to exercise and the like. In other words, an action that improves endurance of the body and an action that suppresses fatigue of the body with respect to an exercise that requires endurance, an exercise that requires repeated muscular work, and exercise in a broad sense including labor It means having. And the anti-fatigue composition of this invention can be made into the anti-fatigue composition excellent in the effect which suppresses the fall of exercise performance by the improvement of the endurance of a body, suppression of fatigue of a body, etc.

Moreover, the anti-fatigue composition in this invention has a lactic acid production inhibitory effect and / or a blood lactic acid accumulation inhibitory effect. In the present invention, the lactic acid production inhibitory action has an action of inhibiting lactic acid production in skeletal muscle and the like. The reduction in blood lactate accumulation refers to the action of suppressing blood lactate accumulation, and the reduction of blood lactate accumulation also includes the action of reducing blood lactate. If the accumulation of lactic acid is suppressed, it is possible to suppress a decrease in muscle activity, perform exercise with stronger intensity, and suppress a decrease in exercise performance.

  The anti-fatigue composition of the present invention can be used as a composition for improving endurance, an anti-fatigue composition during aerobic exercise, a lactic acid production inhibitor, and a blood lactic acid accumulation inhibitor. , And can be provided as pharmaceuticals and quasi drugs.

  Moreover, although the usage-amount (intake amount) per day of the anti-fatigue composition of this invention is not specifically limited, 0.1-100g is preferable in the solid content (solid content conversion amount) of the said fermentation composition, More preferably, it is 1-50 g, More preferably, it is 5-30 g. When the amount used is within the above range, an excellent anti-fatigue effect is exhibited.

  There is no limitation in particular about the form of the anti-fatigue composition of this invention. Usually, it can use with various forms about fermentation compositions, such as a culture solution (fermentation liquid) or a filtrate and concentrated liquid of a fermentation liquid. For example, it can be liquid, solid, powder, granule, granulated granulation and the like.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples as long as the gist of the present invention is not exceeded. “Parts” and “%” are based on mass unless otherwise specified.

[1] Preparation of fermentation composition About 80 parts of water, 1.5 parts of rice bran extract, 1.5 parts of soybean protein, 1.5 parts of phytic acid, 1.5 parts of glucose, 1.5 parts of arginine and an antifoaming agent 0.1 part was added, and after adjusting pH to 8.0 with potassium hydroxide, water was added so that it might become 100 parts in total, and the culture medium was prepared. Next, the medium was sterilized by autoclaving at 121 ° C for 20 minutes, and then cooled to the culture temperature (37 ° C). Then, Bacillus natto was inoculated into the above medium, and cultured for 18 hours at a culture temperature of 37 ° C. without any forced aeration. Thereafter, 3 parts of citric acid were added to the culture medium (fermented product) with respect to 100 parts of the medium to lower the pH to 3.4. Next, after sterilizing by autoclaving at 121 ° C. for 20 minutes, it is cooled to room temperature (about 20 ° C.) and centrifuged (centrifuge; “GRX-250” manufactured by Tommy Seiko Co., Ltd., rotation speed: 5000 rpm, time: 15 The fermented liquor from which solid content was removed was obtained. Next, the fermentation broth was filtered (using # 1 filter paper) to obtain a brown liquid as a fermentation composition. The solid content concentration of this liquid was 10%.

  20 g of the fermented composition obtained as described above was added to 350 g of a cola-flavored beverage to prepare 370 g of an anti-fatigue composition test product A. Further, as a product not containing the above-mentioned fermentation composition (anti-fatigue composition), 20 g of water was added to 350 g of a cola-flavored beverage to prepare 370 g of a control product B.

[2] Performance Evaluation Using the test product A and the control product B obtained by the above method as samples, (1) endurance test and (2) blood lactate measurement were performed by the following methods. .

(1) Endurance test Endurance test was performed using a bicycle ergometer (manufactured by Combi Wellness Co., Ltd. (registered trademark) “POWERMAX-VIII”) with 10 male monitors in their 20s.
In the endurance test, 370 g of the above-mentioned test product A was ingested, and after 1 hour from the ingestion, a bicycle ergometer was run for 10 seconds per minute, and then a rest for 50 seconds was performed for 30 minutes. The endurance test was repeated several times. And the power value (W) in the bicycle ergometer for 10 seconds for every 1 minute was measured. And the graph which plotted the average value of the power value (W) by the 10 male monitors obtained every 1 minute is shown in FIG.

  One week after the endurance test with the test product A, instead of taking the test product A, the control product B was taken, and then the endurance test using a bicycle ergometer was performed in the same manner as described above. And the graph which plotted the power value (W) obtained for every 1 minute is written together in FIG.

(2) Measurement of lactic acid level in blood With respect to the above test product A and the above control product B, the lactic acid level (mmol / l) in blood before and after the endurance test was measured. Specifically, when performing the endurance test, blood is collected from the fingertips immediately before the intake of the test product A or the control product B and immediately after the 30-minute endurance test, and blood before and after the endurance test. The value of lactic acid in it was measured. The measurement results are shown as a graph in FIG. In FIG. 2, the blood concentration immediately before the intake of the test product A or the control product B is expressed as pre, and the blood concentration immediately after the endurance test for 30 minutes is expressed as post.

From the results shown in FIG. 1, the test product A containing the anti-fatigue composition of the present invention improves endurance and suppresses the decrease in exercise performance compared to the control product B containing no anti-fatigue composition. It can be seen that the effect is excellent.
Further, from the results shown in FIG. 2, since the test product A contains the anti-fatigue composition of the present invention, compared to the control product B, the test product A can suppress the production of lactic acid during exercise or the accumulation of lactic acid. It turns out that it is excellent.

The present invention is not limited to the specific examples described above, and can be variously modified examples within the scope of the present invention depending on the purpose and application. That is, the form of the composition is usually
It is a liquid such as a fermented liquid, a filtrate such as a filtrate or a concentrated liquid, or a liquid such as an aqueous solution, but is not limited thereto, and this solid solution is a solid or powder from which a solvent is removed by a known method such as freeze drying or spray drying. Powdered powder may be used. Moreover, it can be set as the powder which impregnated stock solution etc. in the liquid absorption powder, the granulated product which carried out granulation, the tablet which mix | blended other powder components, such as a bulking agent, or a microcapsule. In addition, a product form in which a predetermined container is filled with these undiluted solutions, powdered products, etc., or these can be used alone or mixed with other agents (whether aqueous solutions, oily liquids or powders). There is no particular limitation on whether or not to perform, for example, it may be a portion type, may be filled in a container of another shape, or may be a powder product filled in a stick-like container (bag). Furthermore, you may mix | blend and disperse | distribute to the conventional soft drink, a drink agent, a dairy product, an oil-formation product, etc. In addition, this dispersion | distribution does not ask | require water-in-oil type and an oil-in-water type. Further, other nutritional components, medicinal components, seasoning components, odor components and the like may be blended. Of these, water-soluble components are particularly preferable. It is because it can be set as the product melt | dissolved uniformly.

  Since the present invention has an excellent anti-fatigue property and an action of inhibiting lactic acid production or blood lactate accumulation, it can be applied to foods and drinks containing an anti-fatigue composition. For example, the present invention can be suitably used in the fields of beverages (particularly sports drinks), foods (particularly functional foods, health foods), pharmaceuticals and the like.

Claims (2)

An anti-fatigue composition characterized by using a fermented composition obtained by inoculating natto bacteria on a medium containing rice bran, soybeans, phytic acid and / or a salt thereof, and arginine and fermenting and culturing the same. Manufacturing method . The content of the soybeans, the phytic acid and / or salt thereof, and the arginine in the medium is 50 to 200 parts by mass with respect to 100 parts by mass of the rice bran, respectively. A method for producing an anti-fatigue composition.

Images