NL2032233B1 - Probiotic compositions fermentation formula and method of preparation - Google Patents

Probiotic compositions fermentation formula and method of preparation Download PDF

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NL2032233B1
NL2032233B1 NL2032233A NL2032233A NL2032233B1 NL 2032233 B1 NL2032233 B1 NL 2032233B1 NL 2032233 A NL2032233 A NL 2032233A NL 2032233 A NL2032233 A NL 2032233A NL 2032233 B1 NL2032233 B1 NL 2032233B1
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probiotics
servings
fermentation
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Liu Junhui
Liu Hongyan
Gao Qian
Ye Qingcai
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Anhui Zhongwei Weiyuan Biological Tech Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof

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Abstract

PROBIOTIC COMPOSITIONS FERMENTATION FORMULA AND METHOD OF PREPARATION Probiotic compositions fermentation formula is disclosed, including probiotics, starter 5 culture and single Chinese herb, in which there is bifidobacterium lactics ranging from 30 to 50, lactobacillus rhamnosus ranging from 30 to 50, lactobacillus acidophilus ranging from 30 to 50, bacillus strains ranging from 20 to 40, and streptococcus thermophilus ranging from 20 to 40 for probiotics, yeast powder ranging from 8 to 15 for starter culture, as well as astragalus membranaceus ranging from 10 to 18 for single Chinese herb respectively, according to weight 10 parts. This formula is configured with various Chinese herbal ingredients performing pharmacological actions, most nutrition compositions of which furnish diversified nutrition for the probiotics cultivation, bring a rise in the amount and variety of intestinal probiotics, serve the critical role of balancing mechanism micro ecological environment, eliminate obstacles for the proliferation of intestinal probiotics, enable the inhibition of the cultivation and proliferation of 15 harmful bacteria, as well as the promotion of rapid proliferation of intestinal beneficial bacteria, supplying a solution to the side effect from probiotics to some extent, and exerting certain function on disease treatment and prevention, mechanism immune function regulation and intestinal microbial ecology balance.

Description

PROBIOTIC COMPOSITIONS FERMENTATION FORMULA AND METHOD OF
PREPARATION
TECHNICAL FIELD
Embodiments of the present invention are in the field of probiotics fermentation, and in particular, probiotics compositions fermentation formula, more specifically pertaining to a method of preparing probiotics compositions fermentation formula.
BACKGROUND
Probiotics are characterized by live microorganisms with beneficial effects on host health.
Currently, there are over 20 kinds of probiotics used in China, mainly bifidobacteria, lactobacillus, clostridium butyricum, Saccharomyces boulardii, enterococcus, bacillus licheniformis and bacillus cereus, etc. The beneficial bacteria or fungi in human body and animal body are mainly emerged as lactic acid bacteria, bifidobacteria and lactobacillus acidophilus, etc., extensive applied in bioengineering, food safety and life health. To this day, probiotics found by scientists generally falls into three major categories, including: 1. lactobacillus {such as lactobacillus acidophilus, lactobacillus casei, lactobacillus janus, lactobacillus laman, etc.}; 2. bifidobacteria {such as bifidobacterium longum, bifidobacterium breve, oval bifidobacterial, bifidobacterium thermophilum, etc}; 3. gram-positive cocci {such as Streptococcus faecalis, lactococcus, intermediary Streptococcus, etc.).
No herbal ingredients or only a few herbal ingredients is introduced into most existing probiotics compositions fermentation formulas, leading to the deficiency of nutrition in probiotics, unfavorable growing environment for probiotics, as well as small numbers in amount and variety of intestinal probiotics accordingly, which is not capable of inhibiting the growth and proliferation of harmful bacteria, unfavorable for the rapid proliferation of intestinal beneficial bacteria, and unable to provide a solution to the side effect from probiotics and the function deficiency on disease treatment and prevention, mechanism immune function regulation and intestinal microbial ecology balance, the probiotics compositions fermentation formula and method of preparation are thereby disclosed.
SUMMARY OF THE INVENTION
The subject invention provides probiotic compositions fermentation formula and method of preparation, configured with various Chinese herbal ingredients performing pharmacological actions, most nutrition compositions of which furnish diversified nutrition for the probiotics cultivation, bring a rise in the amount and variety of intestinal probiotics, serve the critical role of balancing mechanism micro ecological environment, eliminate obstacles for the proliferation of intestinal probiotics, enable the inhibition of the cultivation and proliferation of harmful bacteria, as well as the promotion of rapid proliferation of intestinal beneficial bacteria, supplying a solution to the side effect from probiotics to some extent, and exerting certain function on disease treatment and prevention, mechanism immune function regulation and intestinal microbial ecology balance.
To this end, the subject invention provides following technical solution: a probiotics compositions fermentation formula, including probiotics, starter culture and single Chinese herb, in which there is bifidobacterium lactics ranging from 30 to 50, lactobacillus rhamnosus ranging from 30 to 50, lactobacillus acidophilus ranging from 30 to 50, bacillus strains ranging from 20 to 40, and streptococcus thermophilus ranging from 20 to 40 for probiotics, yeast powder ranging from 8 to 15 for starter culture, as well as astragalus membranaceus ranging from 10 to 18, glycyrrhiza uralensis ranging 10 to 18, radix isatidis ranging from 3 to 5, bupleuri radix ranging from 3 to 5, forsythia suspensa ranging 3 to 5, magnolia officinalis ranging from 6 to12, rhubarb ranging from 8 to 15, mirabilite ranging from 5 to 13, lycium barbarum ranging from 6 to 12, rehmannia glutinosa ranging from 5 to 12, and schisandra chinensis ranging 4 to 8 for single
Chinese herb respectively, according to weight parts.
Preferably, according to weight parts, probiotics includes 30 of bifidobacterium lactics, 30 of lactobacillus rhamnosus, 30 of lactobacillus acidophilus, 20 of bacillus strains, and 20 of streptococcus thermophilus, starter culture includes 8 of yeast powder, and single Chinese herb includes 10 of astragalus membranaceus, 10 of glycyrrhiza uralensis, 3 of radix isatidis, 3 of bupleuri radix, 3 of forsythia suspensa, 6 of magnolia officinalis, 8 of rhubarb, 5 of mirabilite, 6 of lycium barbarum, 5 of rehmannia glutinosa, and 4 of schisandra chinensis.
Preferably, according to weight parts, probiotics includes 40 of bifidobacterium lactics, 40 of lactobacillus rhamnosus, 40 of lactobacillus acidophilus, 30 of bacillus strains, and 30 of streptococcus thermophilus, starter culture includes 12 of yeast powder, and single Chinese herb includes 14 of astragalus membranaceus, 14 of glycyrrhiza uralensis, 4 of radix isatidis, 4 of bupleuri radix, 4 of forsythia suspensa, 9 of magnolia officinalis, 11 of rhubarb, 9 of mirabilite, 9 of lycium barbarum, 8 of rehmannia glutinosa, and 6 of schisandra chinensis.
Preferably, according to weight parts, probiotics includes 50 of bifidobacterium lactics, 50 of lactobacillus rhamnosus, 50 of lactobacillus acidophilus, 40 of bacillus strains, and 40 of streptococcus thermophilus, starter culture includes 15 of yeast powder, and single Chinese herb includes 18 of astragalus membranaceus, 18 of glycyrrhiza uralensis, 5 of radix isatidis, 5 of bupleuri radix, 5 of forsythia suspensa, 12 of magnolia officinalis, 15 of rhubarb, 13 of mirabilite, 12 of lycium barbarum, 12 of rehmannia glutinosa, and 8 of schisandra chinensis.
Preferably, the said probiotics are ground into powder, and the probiotics and starter culture powder particles range from 80 mesh to100 mesh.
The present invention also provides a method of preparing probiotics compositions fermentation formula, incorporating the following procedures:
S1, material preparation to weigh each material as spares according to probiotics compositions fermentation formula by weight parts.
S2, material decoction to add an appropriate amount of distilled water to the cooking pot, followed by the single Chinese medicine into distilled water for 45-60 minutes by gentle heat, then to pull out the dregs for a soup, and add 0.5-1% of glucose, 0.3-0.5% of yeast extract, and 0.5-1% of isomaltooligosaccharides respectively by weight, adjusting the PH to 7 with sodium hydroxide, sterilizing the resulting solution under high temperature and pressure at 120 degrees and then cooling as spares.
S3, activation treatment on bifidobacterium lactis, lactobacilius rhamnosus, lactobacitlus acidophilus, bacillus strains, and streptococcus thermophilus in the culture medium.
S4, stirring to take out the cooled single Chinese medicine into stirred tank reactor, with the addition of the starter culture and the activated probiotics to stirred tank reactor for Sminutes to 10 minutes for the intensive mixing of single Chinese medicine and probiotics.
S5. the mixed solution transfer to a constant temperature shaker for cultivation and fermentation in liquid form to gain the probiotics compositions, in which the temperature of the cultivation and fermentation ranges from 25°C to 45°C and the cultivation duration ranges from 2 days to 4 days.
Preferably, according to weight parts, take 14 of membranaceus, 14 of glycyrrhiza uralensis, 4 of radix isatidis, 4 of bupleuri radix, 4 of forsythia suspensa, 9 of magnolia officinalis, 11 of rhubarb, 9 of mirabilite, 9 of lycium barbarum, 8 of rehmannia glutinosa, and 6 of schisandra chinensis and add into 1500ml of distilled water for 50 minutes by gentle heat, then to pull out thedregs fora soup, and add 1% of glucose, 0.5% of yeast extract, 1% of isomaltooligosaccharides respectively by weight, adjusting the PH to 7 with sodium hydroxide, sterilizing the resulting solution under high temperature and pressure at 120 degrees.
Preferably, the said stirred tank reactor is configured with a sterile internal environment, and a stirring rod inside the said stirred tank reactor is set as 80r/min.
Compared with the prior art, the technical effect and advantage of the subject invention is as follows:
Through the configuration of single Chinese herb, the subject invention introduces various
Chinese herbal ingredients performing pharmacological actions, most nutrition compositions of 5 which furnish diversified nutrition for the probiotics cultivation, bring a rise in the amount and variety of intestinal probiotics, serve the critical role of balancing mechanism micro ecological environment, eliminate obstacles for the proliferation of intestinal probiotics, enable the inhibition of the cultivation and proliferation of harmful bacteria, as well as the promotion of rapid proliferation of intestinal beneficial bacteria, supplying a solution to the side effect from probiotics to some extent, and exerting certain function on disease treatment and prevention, mechanism immune function regulation and intestinal microbial ecology balance.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
Technical solutions in embodiments of the subject invention will be clearly and completely explained in the following text. Obviously, the description of the embodiments is merely part of embodiments contained in the invention, not referring to all embodiments contained in the invention. By way of embodiments, and not limitation, embodiments of the present invention are set forth below. It is within the scope of the invention that all other embodiments obtained by ordinary personnel in the art without creative efforts according to the embodiments in the present invention.
Example 1
A probiotics compositions fermentation formula, incorporating probiotics, starter culture and single Chinese herb, according to weight parts, probiotics includes 30 of bifidobacterium lactics, 30 of lactobacillus rhamnosus, 30 of lactobacillus acidophilus, 20 of bacillus strains, and 20 of streptococcus thermophilus, starter culture includes 8 of yeast powder, and single Chinese herb includes 10 of astragalus membranaceus, 10 of glycyrrhiza uralensis, 3 of radix isatidis, 3 of bupleuri radix, 3 of forsythia suspensa, 6 of magnolia officinalis, 8 of rhubarb, 5 of mirabilite, 6 of lycium barbarum, 5 of rehmannia glutinosa, and 4 of schisandra chinensis.
Radix isatidis contains plant proteins, carbonhydrate, sinigrin and various amino acid substances, capable of enabling the promotion of bifidobacterium lactis growth when at low concentration; bupleuri radix is capable of inhibiting the growth of pathogenic microorganisms, and promoting the performance of the nerve center, cardiovascular system and digestive system; forsythia suspensa contains antibacterial components, exerting a strong inhibitory effect on
Escherichia coli, Staphylococcus aureus, etc.; rhubarb plays a significant role in immune regulation, in vivo oxygen radial removal, antibacterial effect and anti-viral function; magnolia officinalis, through its active ingredient, can performs the function of gastric acid secretion inhibition, anti-ulcer, and bactericidal effect on Lactobacillus bulgaricus and hemolytic streptococci, etc.; schisandra chinensis, lycium barbarum and rehmannia glutinosa contain polysaccharides, enabling the promotion of bifidobacterium lactis and normal beneficial intestinal flora growth, and the alance maintenance of the mechanism microbial ecology.
Preferably, said probiotics are ground into powder, and the probiotics and starter culture powder particles range from 80 mesh to100 mesh for easy absorption.
The present invention also provides a method of preparing probiotics compositions fermentation formula, incorporating the following procedures:
S1, material preparation to weigh each material as spares according to probiotics compositions fermentation formula by weight paris. $2, material decoction to add an appropriate amount of distilled water to the cooking pot, followed by the single Chinese medicine into distilled water for 45-60 minutes by gentle heat, then to pull out the dregs for a soup, and add 0.5-1% of glucose, 0.3-0.5% of yeast extract, and 0.5-1% of isomaltooligosaccharides respectively by weight, adjusting the PH to 7 with sodium hydroxide, sterilizing the resulting solution under high temperature and pressure at 120 degrees and then cooling as spares.
S3, activation treatment on bifidobacterium lactis, lactobacillus rhamnosus, lactobacillus acidophilus, bacillus strains, and streptococcus thermophilus in the culture medium.
S4, stirring to take out the cooled single Chinese medicine into stirred tank reactor, with the addition of the starter culture and the activated probiotics to stirred tank reactor for Sminutes to 10 minutes for the intensive mixing of single Chinese medicine and probiotics.
S5. the mixed solution transfer to a constant temperature shaker for cultivation and fermentation in liquid form to gain the probiotics compositions, in which the temperature of the cultivation and fermentation ranges from 25°C to 45°C and the cultivation duration ranges from 2 days to 4 days.
Preferably, according to weight parts, take 14 of membranaceus, 14 of glycyrrhiza uralensis, 4 of radix isatidis, 4 of bupleuri radix, 4 of forsythia suspensa, 9 of magnolia officinalis, 11 of rhubarb, 9 of mirabilite, 9 of lycium barbarum, 8 of rehmannia glutinosa, and 6 of schisandra chinensis and add into 1500ml of distilled water for 50 minutes by gentle heat, then to pull out the dregs for a soup, and add 1% of glucose, 0.5% of yeast extract, 1% of isomaltooligosaccharides respectively by weight, adjusting the PH to 7 with sodium hydroxide, sterilizing the resulting solution under high temperature and pressure at 120 degrees for 15 minutes to 20 minutes.
Preferably, the said stirred tank reactor is configured with a sterile internal environment, and a stirring rod inside the said stirred tank reactor is set as 80r/min.
Example 2
Unlike example 1, according to weight parts, probiotics includes 40 of bifidobacterium lactics, 40 of lactobacillus rhamnosus, 40 of lactobacillus acidophilus, 30 of bacillus strains, and 30 of streptococcus thermophilus, starter culture includes 12 of yeast powder, and single Chinese herb includes 14 of astragalus membranaceus, 14 of glycyrrhiza uralensis, 4 of radix isatidis, 4 of bupleuri radix, 4 of forsythia suspensa, 9 of magnolia officinalis, 11 of rhubarb, 9 of mirabilite, 9 of lycium barbarum, 8 of rehmannia glutinosa, and 6 of schisandra chinensis.
Example 3
Unlike Example 1, according to weight parts, probiotics includes 50 of bifidobacterium lactics, 50 of lactobacillus rhamnosus, 50 of lactobacillus acidophilus, 40 of bacillus strains, and 40 of streptococcus thermophilus, starter culture includes 15 of yeast powder, and single Chinese herb includes 18 of astragalus membranaceus, 18 of glycyrrhiza uralensis, 5 of radix isatidis, 5 of bupleuri radix, 5 of forsythia suspensa, 12 of magnolia officinalis, 15 of rhubarb, 13 of mirabilite, 12 of lycium barbarum, 12 of rehmannia glutinosa, and 8 of schisandra chinensis.
The specific formula data of the three examples of the invention are listed below {according to weight parts):
Toomer [coer [mee ew |e | a | ow eens
ES NW mew | ss | wo ween [os [ow memos | 5 | 5 | ow eee | 0 | © | +
The preparation of the probiotics composition fermentation formula of this invention begins with weighing each material by weight parts as spares according to the probiotics composition fermentation formula, specifically, 14 of membranaceus, 14 of glycyrrhiza uralensis, 4 of radix isatidis, 4 of bupleuri radix, 4 of forsythia suspensa, 9 of magnolia officinalis, 11 of rhubarb, 9 of mirabilite, 9 of lycium barbarum, 8 of rehmannia glutinosa, and 6 of schisandra chinensis, then adding an appropriate amount of distilled water to the cooking pot, followed by the single
Chinese medicine into distilled water for 45-60 minutes by gentle heat, then pulling out the dregs for a soup, and add 0.5-1% of glucose, 0.3-0.5% of yeast extract, and 0.5-1% of isomaltooligosaccharides respectively by weight, adjusting the PH to 7 with sodium hydroxide, sterilizing the resulting solution under high temperature and pressure at 120 degrees and then cooling as spares. Activation treatment is then conducted on bifidobacterium lactis, lactobaciltus rhamnosus, lactobacillus acidophilus, bacillus strains, and streptococcus thermophilus in the culture medium. The cooled single Chinese medicine is subsequently taken out into stirred tank reactor, with the addition of the starter culture and the activated probiotics to stirred tank reactor for 5minutes to 10 minutes for the intensive mixing of single Chinese medicine and probiotics. The mixed solution is then transferred to a constant temperature shaker for cultivation and fermentation in liquid form to gain the probiotics compositions, in which the temperature of the cultivation and fermentation ranges from 25°C to 45°C and the cultivation duration ranges from 2 days to 4 days.
This invention is configured with various Chinese herbal ingredients performing pharmacological actions, most nutrition compositions of which furnish diversified nutrition for the probiotics cultivation, bring a rise in the amount and variety of intestinal probiotics, serve the critical role of balancing mechanism micro ecological environment, eliminate obstacles for the proliferation of intestinal probiotics, enable the inhibition of the cultivation and proliferation of harmful bacteria, as well as the promotion of rapid proliferation of intestinal beneficial bacteria, supplying a solution to the side effect from probiotics to some extent, and exerting certain function on disease treatment and prevention, mechanism immune function regulation and intestinal microbial ecology balance.
Finally, it should be stated that the foregoing descriptions of the embodiments of the invention are intended to illustrate the preferred embodiments and not to limit this invention.
Despite the invention described in detail with reference to the foregoing embodiments, for personnel in the art, modifications may be made on the technical solutions recorded in the foregoing embodiments, or alternations may be made on part of the technical features. The present disclosure may take on any modifications, alterations, and improvements without departing from the spirit and scope of the disclosure.

Claims (8)

CONCLUSIESCONCLUSIONS 1. Fermentatieformule van een probiotische samenstelling, inclusief probiotica, starter en enkele traditionele Chinese geneeskunde, met het kenmerk dat zij probiotica 30-50 porties Bifidobacterium lactis, 30-50 porties Lactobacillus rhamnosus, 30-50 porties Lactobacillus acidophilus, 20-40 porties Bacillus, 20-40 porties Streptococcus thermophilus, 8-15 porties fermentatie poeder, 10-18 porties Radix Astragali, 10-18 porties drop, 3-5 porties Radix Isatidis, 3-5 porties Bupleurum chinense, 3-5 porties Forsythia suspensa, 6-12 porties Magnolia officinalis, 8 -15 porties rabarber, 5-13 porties mirabilite, 6-12 porties wolfberry Rehmannia glutinosa 5-12 porties en Schisandra chinensis 4-8 porties bevat.1. Fermentation formula of a probiotic composition, including probiotics, starter and some traditional Chinese medicine, characterized in that they contain probiotics 30-50 servings of Bifidobacterium lactis, 30-50 servings of Lactobacillus rhamnosus, 30-50 servings of Lactobacillus acidophilus, 20-40 servings of Bacillus , 20-40 servings Streptococcus thermophilus, 8-15 servings fermentation powder, 10-18 servings Radix Astragali, 10-18 servings licorice, 3-5 servings Radix Isatidis, 3-5 servings Bupleurum chinense, 3-5 servings Forsythia suspensa, 6 -12 servings Magnolia officinalis, 8-15 servings rhubarb, 5-13 servings mirabilite, 6-12 servings wolfberry, Rehmannia glutinosa 5-12 servings and Schisandra chinensis 4-8 servings. 2. Fermentatieformule van de probiotische samenstelling volgens conclusie 1, met het kenmerk dat volgens het aantal delen per massa zij, probiotica 30 delen Bifidobacterium lactis, 30 delen Lactobacillus rhamnosus, 30 delen Lactobacillus acidophilus, 20 delen Bacillus, 20 delen Streptococcus thermophilus, 8 delen fermentatie poeder, 10 delen Radix Astragali, 10 delen drop, 3 delen Radix Isatidis, 3 delen bupleurum, 3 delen Forsythia suspensa, 6 delen Magnolia officinalis, 8 delen rabarber, 5 delen mirabilite, 6 delen wolfberry, 5 delen rehmannia en 4 delen Schisandra chinensis bevat.Fermentation formula of the probiotic composition according to claim 1, characterized in that according to the number of parts by mass, probiotics are 30 parts Bifidobacterium lactis, 30 parts Lactobacillus rhamnosus, 30 parts Lactobacillus acidophilus, 20 parts Bacillus, 20 parts Streptococcus thermophilus, 8 parts fermentation powder, 10 parts Radix Astragali, 10 parts licorice, 3 parts Radix Isatidis, 3 parts bupleurum, 3 parts Forsythia suspensa, 6 parts Magnolia officinalis, 8 parts rhubarb, 5 parts mirabilite, 6 parts wolfberry, 5 parts rehmannia and 4 parts Schisandra chinensis contains. 3. Fermentatieformule van de probiotische samenstelling volgens conclusie 1, met het kenmerk dat volgens het aantal delen per massa zij, probiotica 40 delen Bifidobacterium lactis, 40 delen Lactobacillus rhamnosus, 40 delen Lactobacillus acidophilus, 30 delen Bacillus, 30 delen Streptococcus thermophilus, 12 delen fermentatie poeder, 14 delen Radix Astragali, 14 delen drop, 4 delen Radix Isatidis, 4 delen bupleurum, 4 delen Forsythia suspensa, 9 delen Magnolia officinalis, 11 delen rabarber, 9 delen mirabilite, 9 delen wolfberry ‚8 delen rehmannia en 6 delenFermentation formula of the probiotic composition according to claim 1, characterized in that according to the number of parts by mass, probiotics 40 parts Bifidobacterium lactis, 40 parts Lactobacillus rhamnosus, 40 parts Lactobacillus acidophilus, 30 parts Bacillus, 30 parts Streptococcus thermophilus, 12 parts fermentation powder, 14 parts Radix Astragali, 14 parts licorice, 4 parts Radix Isatidis, 4 parts bupleurum, 4 parts Forsythia suspensa, 9 parts Magnolia officinalis, 11 parts rhubarb, 9 parts mirabilite, 9 parts wolfberry‚8 parts rehmannia and 6 parts Schisandra chinensis bevat.Schisandra chinensis contains. 4. Fermentatieformule van de probiotische samenstelling volgens conclusie 1, met het kenmerk dat volgens het aantal massadelen zij, probiotica 50 delen Bifidobacterium lactis, 50 delen Lactobacillus rhamnosus, 50 delen Lactobacillus acidophilus, 40 delen Bacillus, 40 delen Streptococcus thermophilus, 15 delen fermentatie poeder, 18 delen Radix Astragali, 18 delen drop, 5 delen Radix Isatidis, 5 delen bupleurum, 5 delen Forsythia suspensa, 12 delen Magnolia officinalis, 15 delen rabarber, 13 delen mirabilite, 12 delen wolfberry , 12 delen Rehmannia glutinosa en 8 delen Schisandra chinensis bevat.Fermentation formula of the probiotic composition according to claim 1, characterized in that according to the number of parts by mass, probiotics are 50 parts Bifidobacterium lactis, 50 parts Lactobacillus rhamnosus, 50 parts Lactobacillus acidophilus, 40 parts Bacillus, 40 parts Streptococcus thermophilus, 15 parts fermentation powder , 18 parts Radix Astragali, 18 parts licorice, 5 parts Radix Isatidis, 5 parts bupleurum, 5 parts Forsythia suspensa, 12 parts Magnolia officinalis, 15 parts rhubarb, 13 parts mirabilite, 12 parts wolfberry, 12 parts Rehmannia glutinosa and 8 parts Schisandra chinensis contains. 5. Fermentatieformule van de probiotische samenstelling volgens conclusie 1, met het kenmerk dat de probiotica in poedervorm gemalen is en de probiotica en starterpoederdeeltjes zijn ingesteld op 80-100 mesh.The fermentation formula of the probiotic composition according to claim 1, characterized in that the probiotic powder is ground and the probiotics and starter powder particles are adjusted to 80-100 mesh. 6. Werkwijze voor het bereiden van een fermentatieformule van een probiotische samenstelling volgens één van de conclusies 1-5, met het kenmerk dat deze de volgende stappen omvat:Process for preparing a fermentation formula of a probiotic composition according to any one of claims 1-5, characterized in that it comprises the following steps: S1. Voorbereiden van het materiaal volgens de formule van de fermentatie van de probiotische samenstelling, elk materiaal wegen volgens de hoeveelheid delen in massa, en apart zetten;S1. Prepare the material according to the formula of the fermentation of the probiotic composition, weigh each material according to the amount of parts by mass, and set aside; S2. Voor het koken, toevoegen van een passende hoeveelheid gedestilleerd water aan de kookpot, daarna toevoegen van één traditioneel Chinees geneesmiddel aan het gedestilleerde water, gedurende 45-60 minuten laten sudderen, verwijderen van het geneesmiddelresidu, en maken van een soep, toevoegen van 0,5-1% glucose, 0,3-0,5% gistextract, 0,5-1% Isomaltooligosaccharide per gewicht, en aanpassen van de pH tot 7 met natriumhydroxide, steriliseren van de resulterende oplossing onder 120 °C hoge temperatuur en druk, en daarna koelen voor stand-by;S2. Before cooking, add an appropriate amount of distilled water to the cooking pot, then add one traditional Chinese medicine to the distilled water, simmer for 45-60 minutes, remove the medicine residue, and make a soup, adding 0, 5-1% glucose, 0.3-0.5% yeast extract, 0.5-1% Isomaltooligosaccharide by weight, and adjust pH to 7 with sodium hydroxide, sterilize the resulting solution under 120°C high temperature and pressure, and then cooling for standby; S3. Activeren van Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus acidophilus, bacillus en Streptococcus thermophilus in het kweekmedium;S3. Activating Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus acidophilus, bacillus and Streptococcus thermophilus in the culture medium; S4. het afgekoelde enkelvoudige traditionele Chinese geneesmiddel uit de ketel nemen, S overbrengen ervan in de roerketel, en toevoegen van de starter en de geactiveerde probiotica in de roerketel, daarbij roeren gedurende 5-10 minuten, zodat het enkelvoudige traditionele Chinese medicijn en de probiotica volledig kunnen worden gemengd;S4. take the cooled single traditional Chinese medicine from the kettle, transfer it to the stirring kettle, and add the starter and the activated probiotics into the stirring kettle, stirring for 5-10 minutes, so that the single traditional Chinese medicine and the probiotics can fully be mixed; S5. de gemengde oplossing daarna overbrengen naar een constante temperatuurschudtafel voor cultuur, en het fermenteren van de vloeistof om een probiotische samenstelling te verkrijgen, waarbij de temperatuur van de cultuur en de gisting worden gecontroleerd bij 25- 45 °C, en de kweektijd 2-4 dagen is.S5. then transferring the mixed solution to a constant temperature shaking table for culture, and fermenting the liquid to obtain a probiotic composition, controlling the culture temperature and fermentation at 25-45°C, and the culture time 2-4 days is. 7. Werkwijze voor het bereiden van een fermentatieformule van een probiotische samenstelling volgens conclusie 6, met het kenmerk dat volgens het aantal delen per massa, 14 delen Astragalus membranaceus, 14 delen zoethout, 4 delen isatiswortel, 4 delen bupleurumwortel, 4 delen Forsythia suspensa, 9 delen Magnolia officinalis, 11 delen rabarber, 9 delen mirabilite, 9 delen wolfberry fruit, 8 delen Rehmannia glutinosa en 6 delen Schisandra chinensis gebruikt zijn, toevoegen van 1500 mi gedestilleerd water, gedurende 50 minuten laten sudderen, verwijderen van de medicijnresten, maken van de soep, toevoegen van 1500 m! gedestilleerd water en 1% glucose, toevoegen van 0,5% gistextract en 1% Isomaltooligosaccharide per gewicht, instellen van de pH in op 7 met natriumhydroxide en steriliseren van de verkregen oplossing onder 120 °C hoge temperatuur en hoge druk.Process for preparing a fermentation formula of a probiotic composition according to claim 6, characterized in that according to the number of parts by mass, 14 parts of Astragalus membranaceus, 14 parts of licorice root, 4 parts of isatis root, 4 parts of bupleurum root, 4 parts of Forsythia suspensa, 9 parts Magnolia officinalis, 11 parts rhubarb, 9 parts mirabilite, 9 parts wolfberry fruit, 8 parts Rehmannia glutinosa and 6 parts Schisandra chinensis have been used, add 1500 ml distilled water, simmer for 50 minutes, remove drug residues, make the soup, adding 1500 m! distilled water and 1% glucose, adding 0.5% yeast extract and 1% Isomaltooligosaccharide by weight, adjusting the pH to 7 with sodium hydroxide and sterilizing the resulting solution under 120°C high temperature and high pressure. 8. Werkwijze voor het bereiden van een fermentatieformule van een probiotische samenstelling volgens conclusie 6, met het kenmerk dat de omgeving in de roerketel is ingesteld als een steriele omgeving en de rotatiesnelheid van de roerstaaf in de roerketel is ingesteld op 80r/min.A method for preparing a fermentation formula of a probiotic composition according to claim 6, characterized in that the environment in the stirring kettle is set as a sterile environment and the rotation speed of the stirrer bar in the stirring kettle is set to 80r/min.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106266105A (en) * 2016-09-05 2017-01-04 三株福尔制药有限公司 A kind of probiotics fermention Chinese medicine composition of Antialcoholic liver-protecting and its preparation method and application
CN107874251A (en) * 2017-11-14 2018-04-06 广东健来福云健康科技股份有限公司 A kind of eight delicacies probiotic composition with strengthening the spleen and stomach benefit function of intestinal canal

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106266105A (en) * 2016-09-05 2017-01-04 三株福尔制药有限公司 A kind of probiotics fermention Chinese medicine composition of Antialcoholic liver-protecting and its preparation method and application
CN107874251A (en) * 2017-11-14 2018-04-06 广东健来福云健康科技股份有限公司 A kind of eight delicacies probiotic composition with strengthening the spleen and stomach benefit function of intestinal canal

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