CN114191464B - Traditional Chinese medicine microecological preparation for improving duck growth performance and application thereof - Google Patents
Traditional Chinese medicine microecological preparation for improving duck growth performance and application thereof Download PDFInfo
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Abstract
The application discloses a traditional Chinese medicine microecological preparation for improving duck growth performance and application thereof. The traditional Chinese medicine microecological preparation is prepared by adding seven traditional Chinese medicines of red sage root, pilose asiabell root, dyers woad leaf, astragalus root, poria cocos, grifola and liquorice into a fermentation substrate, and fermenting with lactobacillus plantarum, bifidobacterium, clostridium butyricum and bacillus amyloliquefaciens according to a certain mass ratio. Compared with antibiotics such as aureomycin, the application has more obvious advantages, can obviously improve the growth performance and the immune function of cherry valley ducks, and improve serum biochemical and antioxidant indexes. The application is applied to the aspects of growth promotion, disease prevention, antibiosis and the like of livestock and poultry cultivation.
Description
Technical Field
The application relates to a traditional Chinese medicine microecological preparation capable of improving the growth performance and immunity of ducks and application thereof, belonging to the field of feed additives.
Background
The feed additive is a general term for substances added into basic feed to exert the effects of improving the palatability and the nutrition utilization rate of livestock and poultry to the feed, improving the production performance and the product quality of the livestock and poultry, enhancing the disease resistance, the immunity and the like of organisms. The feed additive has small addition amount in basic feed, but has remarkable effects in improving feed conversion rate and livestock and poultry growth performance, ensuring animal health and the like.
The traditional Chinese medicine microecological preparation is a green feed additive obtained by carrying out synergistic fermentation on traditional Chinese medicines and probiotics by using a modern fermentation technology. The probiotics can keep the balance of the host micro-ecological environment, strengthen the immunity of the organism and regulate the intestinal micro-ecological balance. The traditional Chinese medicine is a magnificent of China with long history. Research shows that the Chinese medicine has several active components capable of strengthening body's nourishing, improving body's immunity, resisting bacteria, diminishing inflammation, resisting virus, etc. The traditional Chinese medicine microecological preparation is based on microecological theory, and the preparation work of the traditional Chinese medicine microecological preparation is completed by means of modern microbial fermentation technology, so that the traditional Chinese medicine matrix organisms are transformed, and finally, the corresponding product is obtained. Under the condition, the traditional Chinese medicine composition can be applied to the animal husbandry, so that the toxic and side effects of the traditional Chinese medicine can be greatly reduced, macromolecular impurities in the traditional Chinese medicine can be removed, and beneficial microorganisms can positively influence the health of animal organisms.
In the production of livestock and poultry, the residue of chemical feed additives such as antibiotics and hormone is large, the toxic and side effects are deep, animal-derived food pollution caused by the chemical feed additives such as antibiotics is generally concerned and worried by society, the market demands are difficult to adapt, and green feed additives are gradually becoming the main stream of the market.
Disclosure of Invention
The application aims to provide a traditional Chinese medicine microecological preparation capable of improving the growth performance of ducks, which can remarkably improve the growth performance, immune function, serum biochemical and antioxidant indexes of cherry valley ducks and has positive effects on promoting the growth of meat ducks and promoting immunity.
In order to achieve the above purpose, the present application adopts the following technical scheme: a Chinese medicinal microecological preparation capable of improving growth performance of duck is prepared by selecting antibacterial and liver-protecting Chinese medicinal materials such as Saviae Miltiorrhizae radix, radix Codonopsis, folium Isatidis, radix astragali, poria, polyporus and Glycyrrhrizae radix, drying, pulverizing, adding 1.5 times of water and 15% of nutrition substrate of the mixture of Chinese medicinal materials, mixing, sterilizing at high temperature under high pressure, cooling, inoculating probiotic accounting for 20% of the total mass of the composition, sealing at 37deg.C, fermenting 48 h, air drying at room temperature, and pulverizing; wherein the traditional Chinese medicine consists of 3-5 parts of red sage root, 2-4 parts of pilose asiabell root, 2-4 parts of dyers woad leaf, 2-4 parts of liquorice, 1-3 parts of grifola, 1-3 parts of poria and 1-3 parts of astragalus root in parts by weight; the probiotics consist of lactobacillus plantarum (Lactobacillus plantarum) M III, bifidobacterium bifidum (Bifidobacterium bifidum), clostridium butyricum (Clostridium butyricum) and bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
Preferably, in the probiotic seed liquid, the volume ratio of the lactobacillus plantarum MIII to the bifidobacterium bifidum to the bacillus amyloliquefaciens to the clostridium butyricum is 2-4:2-4:1-3:1-3.
Preferably, the nutrient substrate consists of 2-4 parts of corn starch, 2-4 parts of brown sugar, 2-4 parts of fructo-oligosaccharide, 2-4 parts of lysine and 1-3 parts of talcum powder, wherein the nutrient substrate accounts for 5-30% of the total weight of the traditional Chinese medicine powder and water.
Preferably, the bacillus amyloliquefaciens is a bacillus amyloliquefaciens L2 strain which is isolated from cecum of healthy chickens and has protease and amylase activities; the bacillus amyloliquefaciens L2 discloses biological preservation information in the application patent text with the publication number of CN 113476536A; specifically, the strain is classified and named as bacillus amyloliquefaciens L2, latin literature is named as Bacillus amyloliquefaciens L, the preservation unit is named as China Center for Type Culture Collection (CCTCC), the address is in eight-path 299-No. Wuhan university in Wuchang district of Wuhan, hubei province, the preservation date is 20201005, and the number is M2020570.
Preferably, the probiotic seed liquid comprises 10-50% of the total weight of the fermentation substrate.
Preferably, the fermentation is carried out at a temperature of 37℃for a period of 48 hours.
Preferably, the preparation contains viable bacteria number not less than 1×10 9 CFU/g。
According to a specific embodiment of the application, the optimal method for preparing the traditional Chinese medicine microecological preparation is as follows: pulverizing Saviae Miltiorrhizae radix, radix Codonopsis, folium Isatidis, radix astragali, poria, polyporus and Glycyrrhrizae radix, mixing all the above materials at mass ratio of=3:2:2:2:1:1:1, adding into fermentation matrix, inoculating probiotic strain seed liquid accounting for 20% of the total mass of fermentation matrix, hermetically fermenting at 37deg.C for 48 h, air drying at room temperature, and sieving with 60 mesh sieve.
The fermentation substrate consists of water which is 1.5 times of the total mass of the traditional Chinese medicine and a nutrition substrate which is 15% of the total mass of the mixture of the traditional Chinese medicine and the water, wherein the nutrition substrate comprises corn starch, brown sugar, fructo-oligosaccharide, lysine and talcum powder, and the mass ratio of the nutrition substrate to the corn starch to the brown sugar to the fructo-oligosaccharide to the lysine to the talcum powder is 2:2:2:2:1.
The probiotic bacterial seed liquid comprises lactobacillus plantarum MIII, bifidobacterium, clostridium butyricum and bacillus amyloliquefaciens, and the volume ratio of the probiotic bacterial seed liquid to the bacillus amyloliquefaciens is 2:2:1:1.
In the application, lactobacillus plantarum M III discloses biological preservation information in the application patent document with the publication number of CN 110495522A.
The efficacy of the raw materials selected in the application is as follows:
root of red-rooted salvia: bitter, slightly cold, heart and liver meridian, has the effects of promoting blood circulation, removing blood stasis, dredging meridian, relieving pain, clearing heart fire, relieving restlessness, cooling blood and eliminating carbuncle;
radix codonopsis pilosulae: sweet, calm, enter spleen and lung meridian, invigorate spleen and benefit lung, nourish blood and promote the production of body fluid;
folium Isatidis: bitter, cold, heart and stomach meridian, heat-clearing and detoxicating, blood cooling and spot eliminating;
radix astragali: sweet, slightly warm, enter lung and spleen meridians, tonify qi and raise yang, strengthen superficies and stop sweating, induce diuresis to alleviate edema, promote fluid production and nourish blood, promote stagnancy and relieve arthralgia, remove toxic materials and promote pus discharge, heal sore and promote granulation;
poria cocos: sweet, light, flat, enter heart, lung, spleen and kidney meridians, induce diuresis and excrete dampness, invigorate spleen, calm heart;
polyporus umbellatus). Sweet, light, flat, enter kidney and bladder meridians, induce diuresis and excrete dampness;
licorice root: sweet, flat, heart, lung, spleen and stomach channels, spleen tonifying, qi replenishing, heat and toxic materials clearing away, phlegm eliminating, cough relieving, urgency relieving, pain relieving, and medicines regulating;
bifidobacteria: has the physiological activities of nourishing, inhibiting bacteria, enhancing immunity, protecting liver, improving gastrointestinal function, etc.;
clostridium butyricum: short chain fatty acid is generated in the intestinal tract metabolic process, and has the effects of inhibiting growth or killing pathogenic bacteria in the intestinal tract and promoting growth of beneficial bacteria in the intestinal tract such as lactobacillus and bifidobacterium;
bacillus amyloliquefaciens: can produce abundant amylase, protease and lipase, and can produce polypeptide, lipopeptide and antibacterial protein antibacterial substances;
lactobacillus plantarum M III: has strong antibacterial capability and strong stress resistance.
The application has the beneficial effects that:
1. the addition of the traditional Chinese medicine microecological preparation obviously improves the average weight and average daily gain of cherry valley ducks, reduces the feed-to-weight ratio, and obviously improves the growth performance of cherry valley ducks and the utilization rate of feed;
2. the traditional Chinese medicine microecological preparation can obviously increase spleen and bursa of Fabricius index of cherry valley ducks, and the effect is obviously better than that of aureomycin, which indicates that the traditional Chinese medicine microecological preparation can promote the development of immune organs of cherry valley ducks, thereby enhancing the immune function of cherry valley ducks and replacing antibiotics to a certain extent;
3. the traditional Chinese medicine microecological preparation has obvious antibacterial effects on escherichia coli, staphylococcus epidermidis, staphylococcus aureus and salmonella, and the antibacterial effect of the preparation is even more obvious than that of aureomycin, which indicates that the traditional Chinese medicine microecological preparation can replace antibiotics to a certain extent and is applied to antibiosis and disease prevention in the livestock and poultry breeding industry;
4. the traditional Chinese medicine microecological preparation reduces the MDA level of main products of AST, ALT and lipid peroxidation of cherry valley ducks, balances the GSH-Px of peroxidases in the oxidation-reduction state of organisms, the total level of various antioxidant macromolecules, antioxidant micromolecules and enzymes in a system and the SOD level of a primary substance for removing free radicals of the organisms, and shows that the traditional Chinese medicine microecological preparation can obviously improve serum biochemical and antioxidant indexes of livestock and poultry;
5. the traditional Chinese medicine microecological preparation is selected according with the compatibility basis, most of selected medicines belong to medicine and food homologous traditional Chinese medicines, the selected probiotics have good antibacterial stress resistance, the traditional Chinese medicines and the probiotics can play a synergistic effect in combination, so that the growth of livestock and poultry can be promoted, the immunity can be improved, the residues of feed additives such as antibiotics and growth hormone in animal bodies can be avoided, and the feed additive is a green feed additive with excellent performance.
Drawings
Fig. 1: in-vitro antibacterial effect of traditional Chinese medicine microecological preparation on common pathogenic bacteria [ mean±sd, n=3; the different letters marked on the a-d plots indicate significant differences (p < 0.05), the same letters indicate insignificant differences (p > 0.05) ].
Fig. 2: influence of traditional Chinese medicine microecological preparation on immune organ index of 35-day-old cherry valley ducks [ mean±sd, n=10; the different letters marked on the a-c plots indicate significant differences (p < 0.05), the same letters indicate insignificant differences (p > 0.05) ].
Fig. 3: influence of traditional Chinese medicine microecological preparation on serum biochemical index of 35-day-old cherry valley duck [ mean±sd, n=10; the different letters marked on the a-c plots indicate significant differences (p < 0.05), the same letters indicate insignificant differences (p > 0.05) ].
Fig. 4: influence of traditional Chinese medicine microecological preparation on serum antioxidant index of 35-day-old cherry valley ducks [ mean±sd, n=10; the different letters marked on the a-c plots indicate significant differences (p < 0.05), the same letters indicate insignificant differences (p > 0.05) ].
Detailed Description
See the embodiments for more details. The application is described in further detail below in connection with specific examples which are given solely for the purpose of illustration and are not intended to limit the scope of the application. And without departing from the technical scheme conception of the application, a plurality of variants and modifications can be made, which all fall within the protection scope of the application.
Embodiment case 1: preparation of traditional Chinese medicine microecological preparation
1. Preparation of probiotic seed liquid
Inoculating a small amount of lactobacillus plantarum MIII to an MRS broth culture medium by using an inoculating loop, inoculating a small amount of bacillus amyloliquefaciens L2 to an NB broth culture medium, placing the NB broth culture medium in a constant-temperature oscillator at 37 ℃, and aerobically culturing at 220 rpm to a stable period; inoculating the seed solution cultured to the stationary phase into a corresponding liquid culture medium, and culturing at 37 ℃ and 220 rpm to the stationary phase to obtain seed solution of lactobacillus plantarum MIII and seed solution of bacillus amyloliquefaciens L2 respectively.
Selecting a small amount of bifidobacterium bifidum and clostridium butyricum, respectively inoculating the bifidobacterium bifidum and the clostridium butyricum into MRS and RCM liquid culture media, and carrying out anaerobic culture at 37 ℃ until the bifidobacterium bifidum and the clostridium butyricum reach a stable stage; seed solutions of bifidobacterium bifidum and clostridium butyricum are obtained respectively.
Mixing the lactobacillus plantarum MIII, the bifidobacterium bifidum, the bacillus amyloliquefaciens L2 and the clostridium butyricum seed liquid according to the volume ratio of 2:2:1:1 to obtain the composite probiotic seed liquid.
2. Preparation of traditional Chinese medicine microecological preparation
Selecting antibacterial liver-protecting traditional Chinese medicines (radix salviae miltiorrhizae, radix codonopsis pilosulae, folium isatidis, radix astragali, poria cocos, polyporus umbellatus and liquorice, wherein the mass ratio of the radix astragali to the poria cocos is (3:2:2:2:1:1:1), adding the traditional Chinese medicines into a fermentation substrate [ water which is 1.5 times of the total mass of the traditional Chinese medicines and a nutrient substrate (corn starch: fructo-oligosaccharide: lysine: talcum powder mass ratio of the traditional Chinese medicines (2:2:2:2:1) ] which is 15% of the total mass of the traditional Chinese medicine and water mixture ], inoculating probiotics accounting for 20% of the total mass of the composition, hermetically fermenting the traditional Chinese medicines at 37 ℃ for 48 h, air-drying the traditional Chinese medicines at room temperature, and sieving the traditional Chinese medicines with a 60-mesh sieve for standby.
Embodiment case 2: oxford cup bacteriostasis experiment of traditional Chinese medicine microecological preparation
Test drug:
antibiotics: aureomycin;
commercial microecologics: macleaya cordata;
traditional Chinese medicine microecological preparation: prepared as in example 1;
traditional Chinese medicine mixture: mixing and crushing the traditional Chinese medicine formula in the embodiment 1;
probiotic mixture: mixing lactobacillus plantarum, bifidobacterium bifidum, bacillus amyloliquefaciens L2 and clostridium butyricum in a volume ratio of 2:2:1:1, inoculating to a nutrition substrate (corn starch: brown sugar: fructo-oligosaccharide: lysine: talcum powder mass ratio=2:2:2:2:1), hermetically fermenting at 37 ℃ for 48 h, and air-drying and crushing into bacterial powder.
Diluting four indicator bacteria (Salmonella, staphylococcus epidermidis, staphylococcus aureus and Escherichia coli) fermentation broth with sterile water to 10 -3 Respectively sucking the fermentation liquid of each indicator bacterium diluted by 0.1 mL to a corresponding culture dish by a pipetting gun, and uniformly coating by a coating rod; then using sterile forceps to clamp the sterilized and dried oxford cup, vertically placing the oxford cup on a plate containing indicator bacteria, and lightly applying pressure to ensure that the oxford cup contacts a medium without gaps; then, respectively sucking the test medicines into the corresponding oxford cups by using a pipetting gun, and covering the culture dish cover; carefully placing the culture dish into a constant temperature incubator, culturing at 37 ℃, observing the size of a bacteriostasis zone around the oxford cup after 24 and h, and judging that the bacteriostasis activity is achieved if the bacteriostasis zone is more than or equal to 5 mm.
The in vitro antibacterial test of the application is shown in figure 1, and the result shows that: the antibacterial effect of the traditional Chinese medicine microecological preparation on escherichia coli, staphylococcus epidermidis, staphylococcus aureus and salmonella is obviously superior to that of a single traditional Chinese medicine mixture or a probiotic mixture, which shows that the combined fermentation of the traditional Chinese medicine microecological preparation and the probiotic mixture can actually enhance the antibacterial activity, and the antibacterial effect of the preparation is even more obvious than that of aureomycin, which shows that the preparation can possibly replace antibiotics to a certain extent.
Embodiment 3: influence of traditional Chinese medicine microecological preparation on growth performance, immune organ index and serum index of cherry valley ducks
1. Animal experiment grouping design
200 healthy male cherry valley ducks with the average weight of 73.87+/-2.67 g at 0 days of age are selected, randomly divided into 4 groups of 50 (5 replicates per group and 10 replicates per group), and each duck is weighed, marked and recorded. Blank control group: feeding cherry valley ducks with a basic ration of 35 d; traditional Chinese medicine microecological preparation group: feeding cherry valley ducks with a basic ration 35 d added with 0.2% of traditional Chinese medicine microecological preparation; antibiotic group: feeding cherry valley ducks with a basic ration 35 d added with 0.015% aureomycin; commercial traditional Chinese medicine microecological preparation group: the cherry valley ducks are fed with basic ration 35 d added with 0.2% of the commercial traditional Chinese medicine microecological preparation for poultry.
2. Feeding management
All cherry valley ducks are placed in a duck cage for feeding, and are subjected to 24-hour illumination, free feeding and drinking, insect expelling and disinfection according to the conventional feeding management program of a duck farm.
3. Detection of growth Properties
Weighing before feeding 1, 7, 14, 21, 28 and 35 d morning respectively, recording feed intake and residual feed amount of cherry valley ducks of each test group every day, timely recording death number during feeding, and calculating average weight, average daily gain, average daily feed intake and feed weight ratio of each stage. The calculation formula is as follows: average body weight (g) =randomly weighed total mass/weighed number only; average daily gain (g) = (last weight-initial weight)/experimental days; average daily feed intake (g/d) =total feed intake/experimental days; feed weight ratio (g/g) =average daily feed intake/average daily gain.
4. Determination of immune organ index
At 35 d, all cherry valley ducks were slaughtered, the abdominal feathers were cut off with scissors, the abdominal cavity was carefully cut off, the spleen and bursa were removed, the connective tissue and adipose tissue on the organ surface were carefully cut off, then the on-site weighing was performed, and the immune organ index was calculated according to the following formula: immune organ (spleen or bursa) index (100%) =immune organ (spleen or bursa) fresh weight (g)/cherry valley duck pre-slaughter live weight (kg) ×100%.
5. Detection of serum biochemical index
At 35. 35 d, ducks were collected by the wing vein for about 6.0 mL, allowed to stand at 37℃for 2 h, and then centrifuged at 3000 rpm for 15 min to obtain the supernatant (serum) and stored at-80 ℃. According to the instruction of the kit, the contents of AST, ALT, TP, ALB, TG and T-CHO in serum of cherry valley ducks in each group are detected. And calculating the content of Globulin (GLO) and the white ball ratio (A/G), wherein the calculation formula is as follows: GLO content = TP content-ALB content, a/G = ALB content/GLO content.
6. Detection of oxidation index
Serum samples stored at-80℃were thawed at 4℃and then assayed for levels of SOD, MDA, GSH-Px and T-AOC in cherry valley duck serum, strictly as indicated by the corresponding assay kit.
7. Test results
The addition of the traditional Chinese medicine microecological preparation obviously improves the average weight and average daily gain of cherry valley ducks, reduces the feed-to-weight ratio, and has no obvious influence on average daily feed intake.
Details are given in tables 1-4 below.
Table 1: the influence of the traditional Chinese medicine microecological preparation on the average daily feed intake (g/d) of cherry valley ducks (mean±sd, n=5 repetitions/group, 10 repetitions/group), wherein the lower case letters of the same shoulder marks in the table indicate that the difference is not obvious (p > 0.05);
table 2: the influence of the traditional Chinese medicine microecological preparation on the feed weight ratio (g/g) of cherry valley ducks (mean+ -SD, n=5 repeats/group, 10 repeats), different letters marked in the a-c table show that the difference is obvious (p < 0.05), and the same letters show that the difference is not obvious (p > 0.05);
table 3: compared with other groups, the average daily feed intake of cherry valley ducks in each stage of the test of the traditional Chinese medicine microecological preparation group has no significant difference (p is more than 0.05);
table 4: the material weight ratio of the traditional Chinese medicine microecological preparation group is obviously lower than that of a blank control group, an antibiotic group and a commercial traditional Chinese medicine microecological preparation group (p is less than 0.05) when the traditional Chinese medicine microecological preparation group is in a range of 1-35 d.
As shown in table 1, the average body weight of the traditional Chinese medicine microecological formulation group was significantly higher at the end of feeding 14 d, 28 d and 35 d than the blank group, the antibiotic group and the commercial traditional Chinese medicine microecological formulation group (p < 0.05); as shown in table 2, the average daily gain of the traditional Chinese medicine microecological formulation group at 1-35 d was significantly higher than that of the blank group, the antibiotic group and the commercially available traditional Chinese medicine microecological formulation group (p < 0.05); as shown in table 3, the average daily feed intake of cherry valley ducks in each stage of the test of the traditional Chinese medicine microecological preparation group is not significantly different (p > 0.05) from that of other groups, and meanwhile, as shown in table 4, the feed weight ratio of the traditional Chinese medicine microecological preparation group at 1-35 d is significantly lower than that of the blank control group, the antibiotic group and the commercially available traditional Chinese medicine microecological preparation group (p < 0.05); moreover, the beneficial effect of the traditional Chinese medicine microecological preparation on the growth performance of cherry valley ducks is obviously better than that of aureomycin, which suggests that the preparation has potential to be developed into a feed additive for replacing antibiotics.
The traditional Chinese medicine microecological preparation is supplemented in the basic ration, so that the spleen and bursa of Fabricius index of cherry valley ducks can be obviously increased, and the effect is obviously better than that of aureomycin, which indicates that the traditional Chinese medicine microecological preparation can promote the development of immune organs of cherry valley ducks, thereby enhancing the immune function of cherry valley ducks. As can be seen from fig. 2, at 35 d, the spleen index of cherry valley ducks in the traditional Chinese medicine microecological preparation group is significantly higher than that of the blank group and the antibiotic group (p < 0.05), and is close to that of the traditional Chinese medicine microecological preparation group (p > 0.05); the bursa of Fabricius index of the traditional Chinese medicine microecological preparation group is obviously higher than that of a blank control group, an antibiotic group and a commercial traditional Chinese medicine microecological preparation group (p is less than 0.05).
The traditional Chinese medicine microecological preparation can obviously improve serum biochemical indexes of cherry valley ducks and obviously enhance the oxidation resistance of the cherry valley ducks. ALT and AST are common detection indicators used clinically to determine whether the liver is damaged. TP and ALB can reflect the utilization condition of protein by an organism, and ALB and GLO are one of indexes for measuring the immunity level of the organism. T-CHO and TG levels can reflect the availability of lipids to the body. As can be seen from fig. 3, AST and ALT levels in each group of serum were within the normal range. The serum AST and ALT levels were significantly reduced (p < 0.05) in the traditional chinese microecological group, and the ALB levels were significantly increased (p < 0.05), but were close to the commercial microecological group (p > 0.05), relative to the placebo and antibiotic groups. Relative to the blank control group, the TP and GLO levels and the a/G ratio in the serum of the traditional Chinese medicine microecological preparation group were significantly increased (p < 0.05), the TG content was significantly reduced (p < 0.05), but there was no significant difference (p > 0.05) compared to the antibiotic group and the commercial microecological preparation group. SOD is an important substance for the body to scavenge free radicals. GSH-Px is an important catabolic enzyme for body to remove peroxide. T-AOC shows the total antioxidant capacity of the organism. MDA reflects the degree of lipid peroxidation of the body. As can be seen from fig. 4, the serum levels of GSH-Px, SOD and T-AOC were significantly increased (p < 0.05) and the MDA levels were significantly decreased (p < 0.05) in the group of the traditional Chinese medicine probiotics, compared to the blank group and the antibiotic group. The animal experiment results show that the application can obviously improve the serum biochemical and antioxidant indexes of livestock and poultry.
Claims (5)
1. The application of the traditional Chinese medicine microecological preparation in preparing feed additives for promoting duck growth performance and immune function is characterized in that the traditional Chinese medicine microecological preparation is prepared according to the following method: mixing traditional Chinese medicine powder, and then adding water and a nutrition substrate as a fermentation substrate, wherein the fermentation substrate is composed of 1.5 times of water by weight of the total mass of the traditional Chinese medicine and 15% of nutrition substrate by weight of the total mass of a mixture of the traditional Chinese medicine and the water, and the nutrition substrate is composed of 2-4 parts of corn starch, 2-4 parts of brown sugar, 2-4 parts of fructo-oligosaccharide, 2-4 parts of lysine and 1-3 parts of talcum powder by weight; mixing, sterilizing under high pressure, cooling, inoculating probiotic strain seed liquid into fermentation matrix, fermenting at 35-45deg.C under sealed condition for 30-60 h, air drying, and pulverizing; the probiotics seed liquid is lactobacillus plantarum M III, bifidobacterium, clostridium butyricum and bacillus amyloliquefaciens L2, and the volume ratio is 2-4:2-4:1-3:1-3; the traditional Chinese medicine is prepared from seven traditional Chinese medicinal materials of radix salviae miltiorrhizae, radix codonopsis, dyers woad leaf, radix astragali, poria cocos, polyporus umbellatus and liquorice, and comprises, by weight, 3-5 parts of radix salviae miltiorrhizae, 2-4 parts of radix codonopsis, 2-4 parts of dyers woad leaf, 2-4 parts of liquorice, 1-3 parts of Polyporus umbellatus, 1-3 parts of poria cocos and 1-3 parts of radix astragali.
2. The use according to claim 1, wherein: the bacillus amyloliquefaciens is bacillus amyloliquefaciens L2, and the strain is isolated from cecum of healthy chickens and has protease and amylase activities.
3. The use according to claim 1, wherein: the temperature of the fermentation is 37 ℃ and the time is 48 hours.
4. The use according to claim 1, wherein: the inoculation amount of the probiotic seed liquid is 20% of the total mass of the traditional Chinese medicine and fermentation matrix composition.
5. The use according to claim 1, wherein: the viable count in the preparation is not less than 1×10 9 CFU/g。
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| CN1775241A (en) * | 2005-11-18 | 2006-05-24 | 汤生玲 | Immunity strengthening agent of traditional Chinese herbs for birds |
| CN106344761A (en) * | 2016-08-30 | 2017-01-25 | 福建贝迪药业有限公司 | Fermentation Chinese herbal medicine for improving livestock and poultry immunity and preparing method thereof |
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