MY161418A - Assembly of large nucleic acids - Google Patents

Assembly of large nucleic acids

Info

Publication number
MY161418A
MY161418A MYPI2010001512A MYPI2010001512A MY161418A MY 161418 A MY161418 A MY 161418A MY PI2010001512 A MYPI2010001512 A MY PI2010001512A MY PI2010001512 A MYPI2010001512 A MY PI2010001512A MY 161418 A MY161418 A MY 161418A
Authority
MY
Malaysia
Prior art keywords
assembly
nucleic acids
large nucleic
cassettes
recombination
Prior art date
Application number
MYPI2010001512A
Other languages
English (en)
Inventor
Daniel G Gibson
Gwynedd A Benders
Lei Young
John I Glass
J Craig Venter
Iii Clyde A Hutchison
Hamilton O Smith
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of MY161418A publication Critical patent/MY161418A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
MYPI2010001512A 2007-10-08 2008-10-07 Assembly of large nucleic acids MY161418A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US98354907P 2007-10-29 2007-10-29
US6221408P 2008-01-23 2008-01-23
US2339208P 2008-01-24 2008-01-24
US9627008P 2008-09-11 2008-09-11

Publications (1)

Publication Number Publication Date
MY161418A true MY161418A (en) 2017-04-14

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2010001512A MY161418A (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Country Status (11)

Country Link
US (1) US9593329B2 (https=)
EP (1) EP2207899B1 (https=)
JP (1) JP5618413B2 (https=)
CN (1) CN101932725A (https=)
AU (1) AU2008311000B2 (https=)
CA (1) CA2701923C (https=)
DK (1) DK2207899T3 (https=)
IL (1) IL204892B (https=)
MX (1) MX2010003644A (https=)
MY (1) MY161418A (https=)
WO (1) WO2009048885A2 (https=)

Families Citing this family (31)

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Publication number Priority date Publication date Assignee Title
US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9234226B2 (en) 2008-10-14 2016-01-12 Wayne M. Barnes In vitro recombination methods using 5′ exonuclease
WO2010059763A1 (en) * 2008-11-19 2010-05-27 Amyris Biotechnologies, Inc. Compositions and methods for the assembly of polynucleotides
SG10201400436PA (en) 2009-03-06 2014-06-27 Synthetic Genomics Inc Methods For Cloning And Manipulating Genomes
WO2011012999A1 (en) * 2009-07-31 2011-02-03 Novartis Ag Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP2783000A1 (en) * 2011-11-23 2014-10-01 DSM IP Assets B.V. Nucleic acid assembly system
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
EP2890787B1 (en) * 2012-08-31 2019-05-01 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
CA2893817C (en) * 2012-12-13 2021-03-09 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
MX2015009330A (es) * 2013-01-23 2016-04-15 Novartis Ag Reordenamiento del virus de influenza.
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
EP3433354B1 (en) 2016-03-23 2023-12-27 Synthetic Genomics, Inc. Generation of synthetic genomes
US20210284991A1 (en) * 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
AU2019311199B2 (en) * 2018-07-23 2024-12-19 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
US20220235378A1 (en) * 2019-05-06 2022-07-28 Dsm Ip Assets B.V. Multipartite crispr donor
US20240025939A1 (en) 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
JP2000515011A (ja) * 1996-07-09 2000-11-14 アメリカ合衆国 形質転換関連組換えクローニング
PT1538206E (pt) * 1997-09-16 2010-04-12 Centocor Ortho Biotech Inc Método para a síntese química e construção completa de genes e genomas
WO2000006715A1 (en) 1998-07-27 2000-02-10 Genotypes Inc. Automatic eukaryotic artificial chromosome vector
AU5597200A (en) * 1999-06-07 2000-12-28 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
US7822969B2 (en) * 2001-04-16 2010-10-26 Digimarc Corporation Watermark systems and methods
AU2002332027A1 (en) * 2001-10-04 2003-04-14 The Government Of The United States Of America, As Tandem repeat markers
JP2007534303A (ja) * 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のdna配列を作製するための方法
CA2618699C (en) * 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
CA2618665C (en) 2005-08-11 2012-11-13 J. Craig Venter Institute Method for in vitro recombination
US20070122826A1 (en) * 2005-10-12 2007-05-31 J. Craig Venter Institute, Inc. Minimal bacterial genome
CA2698627A1 (en) 2007-10-05 2009-04-09 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Also Published As

Publication number Publication date
JP2010539994A (ja) 2010-12-24
DK2207899T3 (en) 2018-09-03
US9593329B2 (en) 2017-03-14
AU2008311000A1 (en) 2009-04-16
EP2207899A2 (en) 2010-07-21
AU2008311000B2 (en) 2013-12-19
US20090275086A1 (en) 2009-11-05
MX2010003644A (es) 2010-06-23
WO2009048885A3 (en) 2009-12-30
AU2008311000A2 (en) 2010-06-03
JP5618413B2 (ja) 2014-11-05
IL204892A0 (en) 2010-11-30
CN101932725A (zh) 2010-12-29
CA2701923C (en) 2018-07-10
EP2207899B1 (en) 2018-04-18
WO2009048885A2 (en) 2009-04-16
CA2701923A1 (en) 2009-04-16
IL204892B (en) 2018-02-28
EP2207899A4 (en) 2010-12-01

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