IL204892B - Assembly of large nucleic acids - Google Patents
Assembly of large nucleic acidsInfo
- Publication number
- IL204892B IL204892B IL204892A IL20489210A IL204892B IL 204892 B IL204892 B IL 204892B IL 204892 A IL204892 A IL 204892A IL 20489210 A IL20489210 A IL 20489210A IL 204892 B IL204892 B IL 204892B
- Authority
- IL
- Israel
- Prior art keywords
- assembly
- nucleic acids
- large nucleic
- acids
- nucleic
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Ecology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97838807P | 2007-10-08 | 2007-10-08 | |
| US98354907P | 2007-10-29 | 2007-10-29 | |
| US6221408P | 2008-01-23 | 2008-01-23 | |
| US2339208P | 2008-01-24 | 2008-01-24 | |
| US9627008P | 2008-09-11 | 2008-09-11 | |
| PCT/US2008/079109 WO2009048885A2 (en) | 2007-10-08 | 2008-10-07 | Assembly of large nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL204892A0 IL204892A0 (en) | 2010-11-30 |
| IL204892B true IL204892B (en) | 2018-02-28 |
Family
ID=40549815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL204892A IL204892B (en) | 2007-10-08 | 2010-04-06 | Assembly of large nucleic acids |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9593329B2 (https=) |
| EP (1) | EP2207899B1 (https=) |
| JP (1) | JP5618413B2 (https=) |
| CN (1) | CN101932725A (https=) |
| AU (1) | AU2008311000B2 (https=) |
| CA (1) | CA2701923C (https=) |
| DK (1) | DK2207899T3 (https=) |
| IL (1) | IL204892B (https=) |
| MX (1) | MX2010003644A (https=) |
| MY (1) | MY161418A (https=) |
| WO (1) | WO2009048885A2 (https=) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9267132B2 (en) | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| US9234226B2 (en) | 2008-10-14 | 2016-01-12 | Wayne M. Barnes | In vitro recombination methods using 5′ exonuclease |
| WO2010059763A1 (en) * | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
| SG10201400436PA (en) | 2009-03-06 | 2014-06-27 | Synthetic Genomics Inc | Methods For Cloning And Manipulating Genomes |
| WO2011012999A1 (en) * | 2009-07-31 | 2011-02-03 | Novartis Ag | Reverse genetics systems |
| US20110269119A1 (en) | 2009-10-30 | 2011-11-03 | Synthetic Genomics, Inc. | Encoding text into nucleic acid sequences |
| EP2542676A1 (en) | 2010-03-05 | 2013-01-09 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| WO2012051327A2 (en) | 2010-10-12 | 2012-04-19 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements |
| US12595500B2 (en) | 2011-09-26 | 2026-04-07 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN103945931B (zh) | 2011-09-26 | 2017-03-22 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| EP2783000A1 (en) * | 2011-11-23 | 2014-10-01 | DSM IP Assets B.V. | Nucleic acid assembly system |
| WO2013191950A2 (en) | 2012-06-22 | 2013-12-27 | Monsanto Technology Llc | Unique modular vector design |
| EP2890787B1 (en) * | 2012-08-31 | 2019-05-01 | Synthetic Genomics, Inc. | Crowding agent-induced nucleic acid transfer into a recipient host cell |
| CA2893817C (en) * | 2012-12-13 | 2021-03-09 | Synthetic Genomics, Inc. | Peg-mediated assembly of nucleic acid molecules |
| MX2015009330A (es) * | 2013-01-23 | 2016-04-15 | Novartis Ag | Reordenamiento del virus de influenza. |
| US20140274806A1 (en) | 2013-03-15 | 2014-09-18 | Synthetic Genomics Vaccines | Influenza virus reassortment |
| WO2016007604A1 (en) | 2014-07-09 | 2016-01-14 | Gen9, Inc. | Compositions and methods for site-directed dna nicking and cleaving |
| US10407676B2 (en) | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN106282224B (zh) * | 2015-06-26 | 2020-05-01 | 深圳华大生命科学研究院 | 在靶细胞中构建人工染色体的方法以及真核细胞 |
| EP3763818A1 (en) | 2015-10-06 | 2021-01-13 | Pierce Biotechnology, Inc. | Devices and methods for producing proteins |
| EP3433354B1 (en) | 2016-03-23 | 2023-12-27 | Synthetic Genomics, Inc. | Generation of synthetic genomes |
| US20210284991A1 (en) * | 2016-11-21 | 2021-09-16 | Novozymes A/S | Yeast Cell Extract Assisted Construction of DNA Molecules |
| US10726110B2 (en) * | 2017-03-01 | 2020-07-28 | Seven Bridges Genomics, Inc. | Watermarking for data security in bioinformatic sequence analysis |
| US20210147830A1 (en) | 2018-06-29 | 2021-05-20 | Thermo Fisher Scientific Geneart Gmbh | High throughput assembly of nucleic acid molecules |
| AU2019311199B2 (en) * | 2018-07-23 | 2024-12-19 | Dna Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| US20220235378A1 (en) * | 2019-05-06 | 2022-07-28 | Dsm Ip Assets B.V. | Multipartite crispr donor |
| US20240025939A1 (en) | 2020-03-06 | 2024-01-25 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
| CN113380322B (zh) * | 2021-06-25 | 2023-10-24 | 倍生生物科技(深圳)有限公司 | 人工核酸序列水印编码系统、水印字符串及编码和解码方法 |
| WO2024132094A1 (en) | 2022-12-19 | 2024-06-27 | Thermo Fisher Scientific Geneart Gmbh | Retrieval of sequence-verified nucleic acid molecules |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6348353B1 (en) * | 1995-06-07 | 2002-02-19 | Case Western Reserve University | Artificial mammalian chromosome |
| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| JP2000515011A (ja) * | 1996-07-09 | 2000-11-14 | アメリカ合衆国 | 形質転換関連組換えクローニング |
| PT1538206E (pt) * | 1997-09-16 | 2010-04-12 | Centocor Ortho Biotech Inc | Método para a síntese química e construção completa de genes e genomas |
| WO2000006715A1 (en) | 1998-07-27 | 2000-02-10 | Genotypes Inc. | Automatic eukaryotic artificial chromosome vector |
| AU5597200A (en) * | 1999-06-07 | 2000-12-28 | Cell Genesys, Inc. | Hybrid yeast-bacteria cloning system and uses thereof |
| US7822969B2 (en) * | 2001-04-16 | 2010-10-26 | Digimarc Corporation | Watermark systems and methods |
| AU2002332027A1 (en) * | 2001-10-04 | 2003-04-14 | The Government Of The United States Of America, As | Tandem repeat markers |
| JP2007534303A (ja) * | 2003-05-22 | 2007-11-29 | ユニバーシティー オブ カリフォルニア | 合成遺伝子または他のｄｎａ配列を作製するための方法 |
| CA2618699C (en) * | 2005-08-11 | 2012-10-02 | J. Craig Venter Institute, Inc. | In vitro recombination method |
| CA2618665C (en) | 2005-08-11 | 2012-11-13 | J. Craig Venter Institute | Method for in vitro recombination |
| US20070122826A1 (en) * | 2005-10-12 | 2007-05-31 | J. Craig Venter Institute, Inc. | Minimal bacterial genome |
| CA2698627A1 (en) | 2007-10-05 | 2009-04-09 | Sapphire Energy, Inc. | System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts |
-
2008
- 2008-10-07 CN CN200880119758XA patent/CN101932725A/zh active Pending
- 2008-10-07 EP EP08838436.7A patent/EP2207899B1/en not_active Not-in-force
- 2008-10-07 US US12/247,126 patent/US9593329B2/en active Active
- 2008-10-07 MY MYPI2010001512A patent/MY161418A/en unknown
- 2008-10-07 JP JP2010528976A patent/JP5618413B2/ja not_active Expired - Fee Related
- 2008-10-07 MX MX2010003644A patent/MX2010003644A/es unknown
- 2008-10-07 CA CA2701923A patent/CA2701923C/en active Active
- 2008-10-07 WO PCT/US2008/079109 patent/WO2009048885A2/en not_active Ceased
- 2008-10-07 AU AU2008311000A patent/AU2008311000B2/en not_active Ceased
- 2008-10-07 DK DK08838436.7T patent/DK2207899T3/en active
-
2010
- 2010-04-06 IL IL204892A patent/IL204892B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010539994A (ja) | 2010-12-24 |
| DK2207899T3 (en) | 2018-09-03 |
| US9593329B2 (en) | 2017-03-14 |
| AU2008311000A1 (en) | 2009-04-16 |
| EP2207899A2 (en) | 2010-07-21 |
| AU2008311000B2 (en) | 2013-12-19 |
| US20090275086A1 (en) | 2009-11-05 |
| MY161418A (en) | 2017-04-14 |
| MX2010003644A (es) | 2010-06-23 |
| WO2009048885A3 (en) | 2009-12-30 |
| AU2008311000A2 (en) | 2010-06-03 |
| JP5618413B2 (ja) | 2014-11-05 |
| IL204892A0 (en) | 2010-11-30 |
| CN101932725A (zh) | 2010-12-29 |
| CA2701923C (en) | 2018-07-10 |
| EP2207899B1 (en) | 2018-04-18 |
| WO2009048885A2 (en) | 2009-04-16 |
| CA2701923A1 (en) | 2009-04-16 |
| EP2207899A4 (en) | 2010-12-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |