MXPA06013586A - Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina. - Google Patents

Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.

Info

Publication number
MXPA06013586A
MXPA06013586A MXPA06013586A MXPA06013586A MXPA06013586A MX PA06013586 A MXPA06013586 A MX PA06013586A MX PA06013586 A MXPA06013586 A MX PA06013586A MX PA06013586 A MXPA06013586 A MX PA06013586A MX PA06013586 A MXPA06013586 A MX PA06013586A
Authority
MX
Mexico
Prior art keywords
amino acid
trna
protein
orthogonal
cell
Prior art date
Application number
MXPA06013586A
Other languages
English (en)
Spanish (es)
Inventor
Peter G Schultz
Jianming Xie
Lei Wang
Ning Wu
Glen Spraggon
Original Assignee
Scripss Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripss Res Inst filed Critical Scripss Res Inst
Publication of MXPA06013586A publication Critical patent/MXPA06013586A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
MXPA06013586A 2004-05-25 2005-05-24 Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina. MXPA06013586A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57455404P 2004-05-25 2004-05-25
US60204804P 2004-08-16 2004-08-16
PCT/US2005/018168 WO2005116237A2 (en) 2004-05-25 2005-05-24 Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination

Publications (1)

Publication Number Publication Date
MXPA06013586A true MXPA06013586A (es) 2007-11-09

Family

ID=35451472

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA06013586A MXPA06013586A (es) 2004-05-25 2005-05-24 Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.

Country Status (9)

Country Link
US (1) US7399619B2 (https=)
EP (1) EP1774018A2 (https=)
JP (1) JP2008500050A (https=)
AU (1) AU2005248392A1 (https=)
BR (1) BRPI0511482A (https=)
CA (1) CA2567624A1 (https=)
IL (1) IL179211A0 (https=)
MX (1) MXPA06013586A (https=)
WO (1) WO2005116237A2 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP1649004A4 (en) * 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
US20080171317A1 (en) * 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
CN101048506B (zh) 2004-10-27 2013-06-26 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
BRPI0617191A2 (pt) * 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
WO2007092475A2 (en) 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
KR20080106430A (ko) * 2006-03-09 2008-12-05 더 스크립스 리서치 인스티튜트 진정 세균 숙주 세포 내에서의 직교형 번역 성분의 발현을 위한 시스템
RU2467069C2 (ru) * 2006-03-09 2012-11-20 Зе Скрипс Ресеч Инститьют Система экспрессии компонентов ортогональной трансляции в эубактериальной клетке-хозяине
WO2007109035A2 (en) 2006-03-16 2007-09-27 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
AU2007267950B2 (en) * 2006-05-23 2012-08-16 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
JP5385143B2 (ja) * 2006-09-21 2014-01-08 ザ スクリプス リサーチ インスティチュート 真正細菌における選択的に硫酸化された蛋白質の遺伝的にプログラムされた発現
WO2008073184A2 (en) 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
US8673591B2 (en) 2007-10-25 2014-03-18 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
JP5513398B2 (ja) * 2007-11-02 2014-06-04 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸を含有する蛋白質を使用する指向的進化
CN101939410A (zh) 2007-12-11 2011-01-05 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
CN104651381A (zh) 2008-01-03 2015-05-27 巴斯夫酶有限责任公司 转移酶和氧化还原酶、编码它们的核酸以及其制备和应用方法
EP2249865A4 (en) * 2008-02-08 2012-04-04 Scripps Research Inst BREAKING IMMUNOLOGICAL TOLERANCE WITH A GENETICALLY ENCODED UNNATURAL AMINO ACID
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP4444113B2 (ja) 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
CA2508939A1 (en) * 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
JP5642916B2 (ja) * 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2410331B1 (en) * 2003-06-18 2015-09-23 The Scripps Research Institute Aminoacyl-tRNA synthetase for aminoacylation tRNA with unnatural amino acids
WO2005007624A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
EP1649004A4 (en) * 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
US20060160175A1 (en) * 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
PT1687401E (pt) * 2003-10-14 2013-02-26 Scripps Research Inst Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas
US7741071B2 (en) * 2003-12-18 2010-06-22 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Also Published As

Publication number Publication date
WO2005116237A3 (en) 2007-04-19
AU2005248392A1 (en) 2005-12-08
US20050272121A1 (en) 2005-12-08
BRPI0511482A (pt) 2007-12-26
CA2567624A1 (en) 2005-12-08
EP1774018A2 (en) 2007-04-18
WO2005116237A2 (en) 2005-12-08
JP2008500050A (ja) 2008-01-10
IL179211A0 (en) 2007-03-08
US7399619B2 (en) 2008-07-15

Similar Documents

Publication Publication Date Title
EP1666604B1 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
EP1994165B1 (en) Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
US7399619B2 (en) Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
US8357512B2 (en) Systems for the expression of orthogonal translation components in eubacterial host cells
US8114654B2 (en) Site-specific incorporation of redox active amino acids into proteins
US9624485B2 (en) Genetic incorporation of unnatural amino acids into proteins in mammalian cells
EP1914314B1 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
CN101094916A (zh) 将含有重原子的非天然氨基酸位置特异性地引入蛋白质来确定晶体结构
ES2348487T3 (es) Composiciones de parejas aminoacil-trna sintetasa y trna-lisil ortogonales y usos de las mismas.