AU2005248392A1 - Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination - Google Patents

Info

Publication number

AU2005248392A1

AU2005248392A1 AU2005248392A AU2005248392A AU2005248392A1 AU 2005248392 A1 AU2005248392 A1 AU 2005248392A1 AU 2005248392 A AU2005248392 A AU 2005248392A AU 2005248392 A AU2005248392 A AU 2005248392A AU 2005248392 A1 AU2005248392 A1 AU 2005248392A1

Authority

AU

Australia

Prior art keywords

amino acid

trna

protein

orthogonal

cell

Prior art date

2004-05-25

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Abandoned

Links

• Espacenet
• Global Dossier
• Discuss

• 150000001413 amino acids Chemical class 0.000 title claims description 419
• 108090000623 proteins and genes Proteins 0.000 title claims description 309
• 102000004169 proteins and genes Human genes 0.000 title claims description 288
• 238000010348 incorporation Methods 0.000 title description 50
• 238000005564 crystal structure determination Methods 0.000 title description 4
• 108020004705 Codon Proteins 0.000 claims description 176
• 210000004027 cell Anatomy 0.000 claims description 163
• 238000000034 method Methods 0.000 claims description 144
• 150000007523 nucleic acids Chemical class 0.000 claims description 127
• 102000039446 nucleic acids Human genes 0.000 claims description 119
• 108020004707 nucleic acids Proteins 0.000 claims description 119
• 108091033319 polynucleotide Proteins 0.000 claims description 100
• 102000040430 polynucleotide Human genes 0.000 claims description 100
• 239000002157 polynucleotide Substances 0.000 claims description 99
• 238000013519 translation Methods 0.000 claims description 97
• 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims description 92
• 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims description 92
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 81
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 78
• 229920001184 polypeptide Polymers 0.000 claims description 77
• 102000003960 Ligases Human genes 0.000 claims description 70
• 108090000364 Ligases Proteins 0.000 claims description 70
• 239000000203 mixture Substances 0.000 claims description 59
• 239000013078 crystal Substances 0.000 claims description 41
• 241000203407 Methanocaldococcus jannaschii Species 0.000 claims description 36
• 230000002547 anomalous effect Effects 0.000 claims description 34
• 230000000295 complement effect Effects 0.000 claims description 34
• 241000588724 Escherichia coli Species 0.000 claims description 33
• 238000000338 in vitro Methods 0.000 claims description 29
• 239000013598 vector Substances 0.000 claims description 27
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 26
• 239000004475 Arginine Substances 0.000 claims description 22
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 22
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 22
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 22
• 230000006870 function Effects 0.000 claims description 22
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 21
• 210000003527 eukaryotic cell Anatomy 0.000 claims description 21
• 229930195712 glutamate Natural products 0.000 claims description 18
• 230000008569 process Effects 0.000 claims description 18
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 13
• PZNQZSRPDOEBMS-QMMMGPOBSA-N 4-iodo-L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(I)C=C1 PZNQZSRPDOEBMS-QMMMGPOBSA-N 0.000 claims description 13
• 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims description 13
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 11
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 10
• PEMUHKUIQHFMTH-QMMMGPOBSA-N (2s)-2-amino-3-(4-bromophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=C(Br)C=C1 PEMUHKUIQHFMTH-QMMMGPOBSA-N 0.000 claims description 8
• 101150111020 GLUL gene Proteins 0.000 claims description 8
• 238000002447 crystallographic data Methods 0.000 claims description 7
• UQTZMGFTRHFAAM-ZETCQYMHSA-N 3-iodo-L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(I)=C1 UQTZMGFTRHFAAM-ZETCQYMHSA-N 0.000 claims description 6
• 239000013604 expression vector Substances 0.000 claims description 6
• BABTYIKKTLTNRX-QMMMGPOBSA-N (2s)-2-amino-3-(3-iodophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC(I)=C1 BABTYIKKTLTNRX-QMMMGPOBSA-N 0.000 claims description 5
• BKXVGLPBXYBDDM-QMMMGPOBSA-N (2s)-2-azaniumyl-3-(2-iodophenyl)propanoate Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1I BKXVGLPBXYBDDM-QMMMGPOBSA-N 0.000 claims description 5
• UBPJIGYYLTZEDD-QMMMGPOBSA-N (2s)-2-amino-3-(2-bromo-4-hydroxyphenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1Br UBPJIGYYLTZEDD-QMMMGPOBSA-N 0.000 claims description 4
• JFVLNTLXEZDFHW-QMMMGPOBSA-N (2s)-2-amino-3-(2-bromophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1Br JFVLNTLXEZDFHW-QMMMGPOBSA-N 0.000 claims description 4
• GDMOHOYNMWWBAU-QMMMGPOBSA-N (2s)-2-amino-3-(3-bromophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC(Br)=C1 GDMOHOYNMWWBAU-QMMMGPOBSA-N 0.000 claims description 4
• BMBZSHPNHVPCNG-QMMMGPOBSA-N (2s)-2-amino-3-(4-hydroxy-2-iodophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1I BMBZSHPNHVPCNG-QMMMGPOBSA-N 0.000 claims description 4
• HGWOSUKIFQMEIF-ZETCQYMHSA-N 3-bromo-L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(Br)=C1 HGWOSUKIFQMEIF-ZETCQYMHSA-N 0.000 claims description 4
• 229940024606 amino acid Drugs 0.000 description 386
• 235000001014 amino acid Nutrition 0.000 description 386
• 235000018102 proteins Nutrition 0.000 description 253
• 125000004429 atom Chemical group 0.000 description 129
• 108020004566 Transfer RNA Proteins 0.000 description 119
• 230000014616 translation Effects 0.000 description 82
• 238000001727 in vivo Methods 0.000 description 47
• 125000003275 alpha amino acid group Chemical group 0.000 description 43
• 230000001629 suppression Effects 0.000 description 35
• 102000004190 Enzymes Human genes 0.000 description 33
• 108090000790 Enzymes Proteins 0.000 description 33
• 229940088598 enzyme Drugs 0.000 description 32
• 238000009396 hybridization Methods 0.000 description 30
• 108020004414 DNA Proteins 0.000 description 29
• -1 bromoPhe or iodoPhe) Chemical class 0.000 description 29
• 239000003550 marker Substances 0.000 description 29
• 101000925646 Enterobacteria phage T4 Endolysin Proteins 0.000 description 26
• 238000004519 manufacturing process Methods 0.000 description 24
• 238000002703 mutagenesis Methods 0.000 description 24
• 231100000350 mutagenesis Toxicity 0.000 description 24
• 238000013459 approach Methods 0.000 description 23
• 239000002773 nucleotide Substances 0.000 description 21
• 125000003729 nucleotide group Chemical group 0.000 description 21
• 239000000523 sample Substances 0.000 description 21
• 108020005038 Terminator Codon Proteins 0.000 description 20
• 230000002068 genetic effect Effects 0.000 description 20
• 229910052740 iodine Inorganic materials 0.000 description 20
• 230000004044 response Effects 0.000 description 20
• ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 19
• 241000894007 species Species 0.000 description 19
• 230000015572 biosynthetic process Effects 0.000 description 18
• 238000012216 screening Methods 0.000 description 17
• 241000894006 Bacteria Species 0.000 description 16
• 230000014509 gene expression Effects 0.000 description 16
• 238000003786 synthesis reaction Methods 0.000 description 16
• 238000012360 testing method Methods 0.000 description 16
• 238000003556 assay Methods 0.000 description 15
• 238000004422 calculation algorithm Methods 0.000 description 15
• 239000000243 solution Substances 0.000 description 15
• 238000006467 substitution reaction Methods 0.000 description 15
• 108091060545 Nonsense suppressor Proteins 0.000 description 14
• 235000009697 arginine Nutrition 0.000 description 14
• 230000000694 effects Effects 0.000 description 14
• 235000002374 tyrosine Nutrition 0.000 description 13
• 230000002209 hydrophobic effect Effects 0.000 description 12
• 230000035772 mutation Effects 0.000 description 12
• 230000037361 pathway Effects 0.000 description 12
• 108091028043 Nucleic acid sequence Proteins 0.000 description 11
• 235000013675 iodine Nutrition 0.000 description 11
• 239000013612 plasmid Substances 0.000 description 11
• 229920000642 polymer Polymers 0.000 description 11
• 235000001508 sulfur Nutrition 0.000 description 11
• 229960004441 tyrosine Drugs 0.000 description 11
• 241000238631 Hexapoda Species 0.000 description 10
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 10
• 230000006229 amino acid addition Effects 0.000 description 10
• 239000011630 iodine Substances 0.000 description 10
• 238000000746 purification Methods 0.000 description 10
• 229910052717 sulfur Inorganic materials 0.000 description 10
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
• 241000196324 Embryophyta Species 0.000 description 9
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 9
• NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 9
• 239000011593 sulfur Substances 0.000 description 9
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 9
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 8
• 125000000539 amino acid group Chemical group 0.000 description 8
• 230000006696 biosynthetic metabolic pathway Effects 0.000 description 8
• 238000002425 crystallisation Methods 0.000 description 8
• 230000008025 crystallization Effects 0.000 description 8
• 238000001212 derivatisation Methods 0.000 description 8
• 238000002050 diffraction method Methods 0.000 description 8
• 101150066555 lacZ gene Proteins 0.000 description 8
• 230000002829 reductive effect Effects 0.000 description 8
• 241000205062 Halobacterium Species 0.000 description 7
• 108020004485 Nonsense Codon Proteins 0.000 description 7
• 108091034117 Oligonucleotide Proteins 0.000 description 7
• 150000001875 compounds Chemical class 0.000 description 7
• 108020004999 messenger RNA Proteins 0.000 description 7
• 229960005190 phenylalanine Drugs 0.000 description 7
• 235000008729 phenylalanine Nutrition 0.000 description 7
• 235000013930 proline Nutrition 0.000 description 7
• 238000001742 protein purification Methods 0.000 description 7
• 238000013518 transcription Methods 0.000 description 7
• BEWCSNDRHAIGAY-BYPYZUCNSA-N (2s)-2-amino-4-methyltellanylbutanoic acid Chemical compound C[Te]CC[C@H](N)C(O)=O BEWCSNDRHAIGAY-BYPYZUCNSA-N 0.000 description 6
• 102100025698 Cytosolic carboxypeptidase 4 Human genes 0.000 description 6
• 241000206602 Eukaryota Species 0.000 description 6
• 241000233866 Fungi Species 0.000 description 6
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 6
• 241000700605 Viruses Species 0.000 description 6
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Chemical class Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
• 238000007792 addition Methods 0.000 description 6
• 230000008901 benefit Effects 0.000 description 6
• 230000001413 cellular effect Effects 0.000 description 6
• 230000008859 change Effects 0.000 description 6
• 239000003795 chemical substances by application Substances 0.000 description 6
• 238000002474 experimental method Methods 0.000 description 6
• 235000006109 methionine Nutrition 0.000 description 6
• 230000004048 modification Effects 0.000 description 6
• 238000012986 modification Methods 0.000 description 6
• 239000000758 substrate Substances 0.000 description 6
• 230000035897 transcription Effects 0.000 description 6
• 150000003668 tyrosines Chemical class 0.000 description 6
• 108020005098 Anticodon Proteins 0.000 description 5
• 244000105975 Antidesma platyphyllum Species 0.000 description 5
• 101000932590 Homo sapiens Cytosolic carboxypeptidase 4 Proteins 0.000 description 5
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 5
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 5
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
• 101001033003 Mus musculus Granzyme F Proteins 0.000 description 5
• 239000003153 chemical reaction reagent Substances 0.000 description 5
• 230000001186 cumulative effect Effects 0.000 description 5
• 235000009424 haa Nutrition 0.000 description 5
• 229910052739 hydrogen Inorganic materials 0.000 description 5
• 239000001257 hydrogen Substances 0.000 description 5
• 238000003780 insertion Methods 0.000 description 5
• 230000037431 insertion Effects 0.000 description 5
• 229930182817 methionine Natural products 0.000 description 5
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 5
• 238000002741 site-directed mutagenesis Methods 0.000 description 5
• 239000002904 solvent Substances 0.000 description 5
• 239000000126 substance Substances 0.000 description 5
• 230000001225 therapeutic effect Effects 0.000 description 5
• 238000002424 x-ray crystallography Methods 0.000 description 5
• 241000205042 Archaeoglobus fulgidus Species 0.000 description 4
• 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 4
• 241000195493 Cryptophyta Species 0.000 description 4
• 102000053602 DNA Human genes 0.000 description 4
• 241000701533 Escherichia virus T4 Species 0.000 description 4
• 102000018233 Fibroblast Growth Factor Human genes 0.000 description 4
• 108050007372 Fibroblast Growth Factor Proteins 0.000 description 4
• WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
• 241000204933 Haloferax volcanii Species 0.000 description 4
• OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 4
• 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 4
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 4
• 241001465754 Metazoa Species 0.000 description 4
• 241001529871 Methanococcus maripaludis Species 0.000 description 4
• 241000204641 Methanopyrus kandleri Species 0.000 description 4
• 241001148023 Pyrococcus abyssi Species 0.000 description 4
• 241000205156 Pyrococcus furiosus Species 0.000 description 4
• 241000522615 Pyrococcus horikoshii Species 0.000 description 4
• 108091006629 SLC13A2 Proteins 0.000 description 4
• 241000205091 Sulfolobus solfataricus Species 0.000 description 4
• 241000160715 Sulfolobus tokodaii Species 0.000 description 4
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical group O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
• 230000001851 biosynthetic effect Effects 0.000 description 4
• 229910052794 bromium Inorganic materials 0.000 description 4
• 239000000872 buffer Substances 0.000 description 4
• 229910052799 carbon Inorganic materials 0.000 description 4
• 230000010261 cell growth Effects 0.000 description 4
• 239000011549 crystallization solution Substances 0.000 description 4
• 238000013480 data collection Methods 0.000 description 4
• 229940126864 fibroblast growth factor Drugs 0.000 description 4
• 230000037433 frameshift Effects 0.000 description 4
• 150000002308 glutamine derivatives Chemical class 0.000 description 4
• 239000001963 growth medium Substances 0.000 description 4
• 150000004820 halides Chemical class 0.000 description 4
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
• 150000002500 ions Chemical class 0.000 description 4
• 239000000463 material Substances 0.000 description 4
• 238000005259 measurement Methods 0.000 description 4
• 239000002609 medium Substances 0.000 description 4
• 229910052751 metal Inorganic materials 0.000 description 4
• 239000002184 metal Substances 0.000 description 4
• 230000010355 oscillation Effects 0.000 description 4
• 239000013641 positive control Substances 0.000 description 4
• 238000002708 random mutagenesis Methods 0.000 description 4
• 230000006798 recombination Effects 0.000 description 4
• 238000005215 recombination Methods 0.000 description 4
• 238000002791 soaking Methods 0.000 description 4
• 230000002103 transcriptional effect Effects 0.000 description 4
• KYNFOMQIXZUKRK-UHFFFAOYSA-N 2,2'-dithiodiethanol Chemical compound OCCSSCCO KYNFOMQIXZUKRK-UHFFFAOYSA-N 0.000 description 3
• 241000193830 Bacillus <bacterium> Species 0.000 description 3
• 229910016523 CuKa Inorganic materials 0.000 description 3
• WHUUTDBJXJRKMK-GSVOUGTGSA-N D-glutamic acid Chemical compound OC(=O)[C@H](N)CCC(O)=O WHUUTDBJXJRKMK-GSVOUGTGSA-N 0.000 description 3
• 241000186394 Eubacterium Species 0.000 description 3
• 108090000385 Fibroblast growth factor 7 Proteins 0.000 description 3
• 102000003972 Fibroblast growth factor 7 Human genes 0.000 description 3
• 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 3
• 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 3
• 108010002352 Interleukin-1 Proteins 0.000 description 3
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 3
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 3
• 241000124008 Mammalia Species 0.000 description 3
• 238000007476 Maximum Likelihood Methods 0.000 description 3
• 241000205274 Methanosarcina mazei Species 0.000 description 3
• 241000736843 Pyrobaculum aerophilum Species 0.000 description 3
• RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 3
• RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 3
• 102000013275 Somatomedins Human genes 0.000 description 3
• 241000489996 Thermoplasma volcanium Species 0.000 description 3
• 241000589499 Thermus thermophilus Species 0.000 description 3
• 239000007983 Tris buffer Substances 0.000 description 3
• 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 3
• 102100040247 Tumor necrosis factor Human genes 0.000 description 3
• 238000002441 X-ray diffraction Methods 0.000 description 3
• 238000010521 absorption reaction Methods 0.000 description 3
• 239000002253 acid Substances 0.000 description 3
• 235000004279 alanine Nutrition 0.000 description 3
• 235000008206 alpha-amino acids Nutrition 0.000 description 3
• 238000004458 analytical method Methods 0.000 description 3
• 125000003118 aryl group Chemical group 0.000 description 3
• 229960001230 asparagine Drugs 0.000 description 3
• WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
• 229960005091 chloramphenicol Drugs 0.000 description 3
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
• 235000018417 cysteine Nutrition 0.000 description 3
• 238000012217 deletion Methods 0.000 description 3
• 230000037430 deletion Effects 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 239000006185 dispersion Substances 0.000 description 3
• 241001493065 dsRNA viruses Species 0.000 description 3
• 230000007613 environmental effect Effects 0.000 description 3
• 239000012634 fragment Substances 0.000 description 3
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
• 235000004554 glutamine Nutrition 0.000 description 3
• 239000003262 industrial enzyme Substances 0.000 description 3
• 230000002757 inflammatory effect Effects 0.000 description 3
• 230000003993 interaction Effects 0.000 description 3
• 239000000543 intermediate Substances 0.000 description 3
• 238000002955 isolation Methods 0.000 description 3
• 125000000468 ketone group Chemical group 0.000 description 3
• 229920002521 macromolecule Polymers 0.000 description 3
• 210000004962 mammalian cell Anatomy 0.000 description 3
• 239000011159 matrix material Substances 0.000 description 3
• 230000007246 mechanism Effects 0.000 description 3
• 238000012269 metabolic engineering Methods 0.000 description 3
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 3
• 244000005700 microbiome Species 0.000 description 3
• 239000002245 particle Substances 0.000 description 3
• 150000002994 phenylalanines Chemical class 0.000 description 3
• 229920000570 polyether Polymers 0.000 description 3
• 150000003148 prolines Chemical class 0.000 description 3
• 238000003498 protein array Methods 0.000 description 3
• 230000010076 replication Effects 0.000 description 3
• 229910052711 selenium Inorganic materials 0.000 description 3
• 239000011669 selenium Substances 0.000 description 3
• 229960002718 selenomethionine Drugs 0.000 description 3
• 230000035945 sensitivity Effects 0.000 description 3
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
• 231100000765 toxin Toxicity 0.000 description 3
• 239000003053 toxin Substances 0.000 description 3
• 108700012359 toxins Proteins 0.000 description 3
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
• 102000003298 tumor necrosis factor receptor Human genes 0.000 description 3
• MJZAZMKENKZBAJ-NEYKFGMSSA-N tyrosyladenylate Chemical compound C([C@H](N)C(=O)NS(=O)(=O)OC[C@H]1[C@@H]([C@@H](O)[C@@H](O1)N1C2=NC=NC(N)=C2N=C1)O)C1=CC=C(O)C=C1 MJZAZMKENKZBAJ-NEYKFGMSSA-N 0.000 description 3
• 241000701447 unidentified baculovirus Species 0.000 description 3
• 238000011179 visual inspection Methods 0.000 description 3
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
• ULBLZIPFWGIOJF-QMMMGPOBSA-N (2s)-2-(bromoamino)-3-phenylpropanoic acid Chemical compound OC(=O)[C@@H](NBr)CC1=CC=CC=C1 ULBLZIPFWGIOJF-QMMMGPOBSA-N 0.000 description 2
• 108010052418 (N-(2-((4-((2-((4-(9-acridinylamino)phenyl)amino)-2-oxoethyl)amino)-4-oxobutyl)amino)-1-(1H-imidazol-4-ylmethyl)-1-oxoethyl)-6-(((-2-aminoethyl)amino)methyl)-2-pyridinecarboxamidato) iron(1+) Proteins 0.000 description 2
• 241000203069 Archaea Species 0.000 description 2
• 208000002109 Argyria Diseases 0.000 description 2
• 241000238421 Arthropoda Species 0.000 description 2
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 2
• WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
• 102100021943 C-C motif chemokine 2 Human genes 0.000 description 2
• 101710155857 C-C motif chemokine 2 Proteins 0.000 description 2
• 108010029697 CD40 Ligand Proteins 0.000 description 2
• 102100032937 CD40 ligand Human genes 0.000 description 2
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
• 108010071942 Colony-Stimulating Factors Proteins 0.000 description 2
• 101710136772 Crambin Proteins 0.000 description 2
• 239000004971 Cross linker Substances 0.000 description 2
• 102000004127 Cytokines Human genes 0.000 description 2
• 108090000695 Cytokines Proteins 0.000 description 2
• FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 2
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
• RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
• 238000004252 FT/ICR mass spectrometry Methods 0.000 description 2
• 241000193385 Geobacillus stearothermophilus Species 0.000 description 2
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
• 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 2
• 102100021866 Hepatocyte growth factor Human genes 0.000 description 2
• AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 2
• DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
• 108700005091 Immunoglobulin Genes Proteins 0.000 description 2
• 101000668058 Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) RNA-directed RNA polymerase catalytic subunit Proteins 0.000 description 2
• 108090001061 Insulin Proteins 0.000 description 2
• 102000004877 Insulin Human genes 0.000 description 2
• 108010050904 Interferons Proteins 0.000 description 2
• 102000014150 Interferons Human genes 0.000 description 2
• 108010002350 Interleukin-2 Proteins 0.000 description 2
• 108010063738 Interleukins Proteins 0.000 description 2
• 102000015696 Interleukins Human genes 0.000 description 2
• WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
• WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 2
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
• ZFOMKMMPBOQKMC-KXUCPTDWSA-N L-pyrrolysine Chemical compound C[C@@H]1CC=N[C@H]1C(=O)NCCCC[C@H]([NH3+])C([O-])=O ZFOMKMMPBOQKMC-KXUCPTDWSA-N 0.000 description 2
• ZKZBPNGNEQAJSX-REOHCLBHSA-N L-selenocysteine Chemical compound [SeH]C[C@H](N)C(O)=O ZKZBPNGNEQAJSX-REOHCLBHSA-N 0.000 description 2
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
• 241000209510 Liliopsida Species 0.000 description 2
• 102000003820 Lipoxygenases Human genes 0.000 description 2
• 108090000128 Lipoxygenases Proteins 0.000 description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
• 239000004472 Lysine Substances 0.000 description 2
• 108010092041 Lysine-tRNA Ligase Proteins 0.000 description 2
• 102000017737 Lysine-tRNA Ligase Human genes 0.000 description 2
• 241001302042 Methanothermobacter thermautotrophicus Species 0.000 description 2
• 102000016943 Muramidase Human genes 0.000 description 2
• 108010014251 Muramidase Proteins 0.000 description 2
• 241000699670 Mus sp. Species 0.000 description 2
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
• 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
• 108091005461 Nucleic proteins Proteins 0.000 description 2
• 108700020796 Oncogene Proteins 0.000 description 2
• 102000043276 Oncogene Human genes 0.000 description 2
• 239000004721 Polyphenylene oxide Substances 0.000 description 2
• RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
• SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
• BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
• 102000019197 Superoxide Dismutase Human genes 0.000 description 2
• 108010012715 Superoxide dismutase Proteins 0.000 description 2
• MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
• 241000204673 Thermoplasma acidophilum Species 0.000 description 2
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
• 239000004473 Threonine Substances 0.000 description 2
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
• 229910052770 Uranium Inorganic materials 0.000 description 2
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
• 230000003213 activating effect Effects 0.000 description 2
• 239000002671 adjuvant Substances 0.000 description 2
• 238000001042 affinity chromatography Methods 0.000 description 2
• 150000001299 aldehydes Chemical class 0.000 description 2
• 150000001371 alpha-amino acids Chemical class 0.000 description 2
• 230000004075 alteration Effects 0.000 description 2
• 125000003277 amino group Chemical group 0.000 description 2
• 235000009582 asparagine Nutrition 0.000 description 2
• 238000003149 assay kit Methods 0.000 description 2
• 229910052788 barium Inorganic materials 0.000 description 2
• 230000003115 biocidal effect Effects 0.000 description 2
• 230000000903 blocking effect Effects 0.000 description 2
• GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
• 150000001720 carbohydrates Chemical group 0.000 description 2
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
• 238000005277 cation exchange chromatography Methods 0.000 description 2
• 230000004700 cellular uptake Effects 0.000 description 2
• 238000006243 chemical reaction Methods 0.000 description 2
• 239000000460 chlorine Substances 0.000 description 2
• 238000004587 chromatography analysis Methods 0.000 description 2
• 238000010367 cloning Methods 0.000 description 2
• 230000000052 comparative effect Effects 0.000 description 2
• 102000006834 complement receptors Human genes 0.000 description 2
• 108010047295 complement receptors Proteins 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 239000002577 cryoprotective agent Substances 0.000 description 2
• 230000002950 deficient Effects 0.000 description 2
• 238000001514 detection method Methods 0.000 description 2
• VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
• 239000003623 enhancer Substances 0.000 description 2
• 241001233957 eudicotyledons Species 0.000 description 2
• BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
• 238000002523 gelfiltration Methods 0.000 description 2
• 235000013922 glutamic acid Nutrition 0.000 description 2
• 239000004220 glutamic acid Substances 0.000 description 2
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
• 239000005090 green fluorescent protein Substances 0.000 description 2
• 239000003102 growth factor Substances 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 230000006801 homologous recombination Effects 0.000 description 2
• 238000002744 homologous recombination Methods 0.000 description 2
• 229930195733 hydrocarbon Natural products 0.000 description 2
• 150000002430 hydrocarbons Chemical class 0.000 description 2
• 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
• 208000015181 infectious disease Diseases 0.000 description 2
• 206010022000 influenza Diseases 0.000 description 2
• 229940125396 insulin Drugs 0.000 description 2
• 229940047124 interferons Drugs 0.000 description 2
• 229940047122 interleukins Drugs 0.000 description 2
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
• 229960000310 isoleucine Drugs 0.000 description 2
• 239000003446 ligand Substances 0.000 description 2
• 239000004325 lysozyme Substances 0.000 description 2
• 229960000274 lysozyme Drugs 0.000 description 2
• 235000010335 lysozyme Nutrition 0.000 description 2
• 230000037353 metabolic pathway Effects 0.000 description 2
• 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
• 239000006151 minimal media Substances 0.000 description 2
• 238000010369 molecular cloning Methods 0.000 description 2
• 210000001616 monocyte Anatomy 0.000 description 2
• 210000000440 neutrophil Anatomy 0.000 description 2
• 238000007899 nucleic acid hybridization Methods 0.000 description 2
• 239000002853 nucleic acid probe Substances 0.000 description 2
• 235000015097 nutrients Nutrition 0.000 description 2
• 229910052760 oxygen Inorganic materials 0.000 description 2
• 239000000546 pharmaceutical excipient Substances 0.000 description 2
• BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 2
• 239000008057 potassium phosphate buffer Substances 0.000 description 2
• OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 2
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
• 238000012545 processing Methods 0.000 description 2
• 238000001243 protein synthesis Methods 0.000 description 2
• 230000005855 radiation Effects 0.000 description 2
• 108091007054 readthrough proteins Proteins 0.000 description 2
• 102000005962 receptors Human genes 0.000 description 2
• 108020003175 receptors Proteins 0.000 description 2
• 150000003839 salts Chemical class 0.000 description 2
• 235000016491 selenocysteine Nutrition 0.000 description 2
• 229940055619 selenocysteine Drugs 0.000 description 2
• ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 2
• 230000019491 signal transduction Effects 0.000 description 2
• 239000012064 sodium phosphate buffer Substances 0.000 description 2
• 238000010561 standard procedure Methods 0.000 description 2
• 125000001424 substituent group Chemical group 0.000 description 2
• 125000004434 sulfur atom Chemical group 0.000 description 2
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
• 210000001519 tissue Anatomy 0.000 description 2
• 231100000331 toxic Toxicity 0.000 description 2
• 230000002588 toxic effect Effects 0.000 description 2
• 108091006106 transcriptional activators Proteins 0.000 description 2
• 230000012863 translational readthrough Effects 0.000 description 2
• 241001515965 unidentified phage Species 0.000 description 2
• 229940035893 uracil Drugs 0.000 description 2
• VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
• 229960005486 vaccine Drugs 0.000 description 2
• 239000004474 valine Substances 0.000 description 2
• 239000003643 water by type Substances 0.000 description 2
• DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 1
• UZEGYEIVOYIUDU-SECBINFHSA-N (2r)-2-amino-2-hydrazinyl-3-(4-hydroxyphenyl)propanoic acid Chemical compound NN[C@@](N)(C(O)=O)CC1=CC=C(O)C=C1 UZEGYEIVOYIUDU-SECBINFHSA-N 0.000 description 1
• VKYBWTVHQHFSJL-ZATYTLRZSA-N (2s)-2,5-diamino-5-oxopentanoic acid;(2s)-pyrrolidine-2-carboxylic acid Chemical compound OC(=O)[C@@H]1CCCN1.OC(=O)[C@@H](N)CCC(N)=O VKYBWTVHQHFSJL-ZATYTLRZSA-N 0.000 description 1
• HUOYGRNYKOUAFE-QMMMGPOBSA-N (2s)-2-(iodoamino)-3-phenylpropanoic acid Chemical compound OC(=O)[C@@H](NI)CC1=CC=CC=C1 HUOYGRNYKOUAFE-QMMMGPOBSA-N 0.000 description 1
• RWLSBXBFZHDHHX-VIFPVBQESA-N (2s)-2-(naphthalen-2-ylamino)propanoic acid Chemical compound C1=CC=CC2=CC(N[C@@H](C)C(O)=O)=CC=C21 RWLSBXBFZHDHHX-VIFPVBQESA-N 0.000 description 1
• YYTDJPUFAVPHQA-VKHMYHEASA-N (2s)-2-amino-3-(2,3,4,5,6-pentafluorophenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=C(F)C(F)=C(F)C(F)=C1F YYTDJPUFAVPHQA-VKHMYHEASA-N 0.000 description 1
• ALBODLTZUXKBGZ-JUUVMNCLSA-N (2s)-2-amino-3-phenylpropanoic acid;(2s)-2,6-diaminohexanoic acid Chemical compound NCCCC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CC1=CC=CC=C1 ALBODLTZUXKBGZ-JUUVMNCLSA-N 0.000 description 1
• DXAUAWUGCKCSFC-NSHDSACASA-N (2s)-3-phenyl-2-(prop-2-ynoxyamino)propanoic acid Chemical compound C#CCON[C@H](C(=O)O)CC1=CC=CC=C1 DXAUAWUGCKCSFC-NSHDSACASA-N 0.000 description 1
• BNIFSVVAHBLNTN-XKKUQSFHSA-N (2s)-4-amino-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-1-[(2s)-4-amino-2-[[2-[[(2s)-2-[[(2s)-2-[[(2s)-1-[(2s)-6-amino-2-[[(2s)-2-[[(2s)-2-[[(2s,3r)-2-amino-3-hydroxybutanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]hexan Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O)CCC1 BNIFSVVAHBLNTN-XKKUQSFHSA-N 0.000 description 1
• WHTVZRBIWZFKQO-AWEZNQCLSA-N (S)-chloroquine Chemical compound ClC1=CC=C2C(N[C@@H](C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-AWEZNQCLSA-N 0.000 description 1
• YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
• 229940035437 1,3-propanediol Drugs 0.000 description 1
• RXMWXENJQAINCC-DMTCNVIQSA-N 2,5-didehydro-D-gluconic acid Chemical compound OCC(=O)[C@@H](O)[C@H](O)C(=O)C(O)=O RXMWXENJQAINCC-DMTCNVIQSA-N 0.000 description 1
• RXMWXENJQAINCC-UHFFFAOYSA-N 2,5-diketo-D-gluconic acid Natural products OCC(=O)C(O)C(O)C(=O)C(O)=O RXMWXENJQAINCC-UHFFFAOYSA-N 0.000 description 1
• 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
• JZRBSTONIYRNRI-VIFPVBQESA-N 3-methylphenylalanine Chemical compound CC1=CC=CC(C[C@H](N)C(O)=O)=C1 JZRBSTONIYRNRI-VIFPVBQESA-N 0.000 description 1
• IRZQDMYEJPNDEN-UHFFFAOYSA-N 3-phenyl-2-aminobutanoic acid Natural products OC(=O)C(N)C(C)C1=CC=CC=C1 IRZQDMYEJPNDEN-UHFFFAOYSA-N 0.000 description 1
• HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
• 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
• 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
• PQSUYGKTWSAVDQ-UHFFFAOYSA-N Aldosterone Natural products C1CC2C3CCC(C(=O)CO)C3(C=O)CC(O)C2C2(C)C1=CC(=O)CC2 PQSUYGKTWSAVDQ-UHFFFAOYSA-N 0.000 description 1
• PQSUYGKTWSAVDQ-ZVIOFETBSA-N Aldosterone Chemical compound C([C@@]1([C@@H](C(=O)CO)CC[C@H]1[C@@H]1CC2)C=O)[C@H](O)[C@@H]1[C@]1(C)C2=CC(=O)CC1 PQSUYGKTWSAVDQ-ZVIOFETBSA-N 0.000 description 1
• 108700023418 Amidases Proteins 0.000 description 1
• 108090000531 Amidohydrolases Proteins 0.000 description 1
• 102000004092 Amidohydrolases Human genes 0.000 description 1
• 108010008830 Amino Acid Isomerases Proteins 0.000 description 1
• 102000006534 Amino Acid Isomerases Human genes 0.000 description 1
• 241000024188 Andala Species 0.000 description 1
• 102400000068 Angiostatin Human genes 0.000 description 1
• 108010079709 Angiostatins Proteins 0.000 description 1
• 102000007592 Apolipoproteins Human genes 0.000 description 1
• 108010071619 Apolipoproteins Proteins 0.000 description 1
• 108010083590 Apoproteins Proteins 0.000 description 1
• 102000006410 Apoproteins Human genes 0.000 description 1
• OZDNDGXASTWERN-CTNGQTDRSA-N Apovincamine Chemical compound C1=CC=C2C(CCN3CCC4)=C5[C@@H]3[C@]4(CC)C=C(C(=O)OC)N5C2=C1 OZDNDGXASTWERN-CTNGQTDRSA-N 0.000 description 1
• 101000716807 Arabidopsis thaliana Protein SCO1 homolog 1, mitochondrial Proteins 0.000 description 1
• 241000712891 Arenavirus Species 0.000 description 1
• 241000228212 Aspergillus Species 0.000 description 1
• 101800001288 Atrial natriuretic factor Proteins 0.000 description 1
• 102400001282 Atrial natriuretic peptide Human genes 0.000 description 1
• 101800001890 Atrial natriuretic peptide Proteins 0.000 description 1
• 241000271566 Aves Species 0.000 description 1
• 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
• 102100021277 Beta-secretase 2 Human genes 0.000 description 1
• 101710150190 Beta-secretase 2 Proteins 0.000 description 1
• 241000255789 Bombyx mori Species 0.000 description 1
• BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
• 101500028594 Bos taurus Neurophysin 2 Proteins 0.000 description 1
• 238000009010 Bradford assay Methods 0.000 description 1
• 241000219198 Brassica Species 0.000 description 1
• 235000011331 Brassica Nutrition 0.000 description 1
• 102100032367 C-C motif chemokine 5 Human genes 0.000 description 1
• 102100032366 C-C motif chemokine 7 Human genes 0.000 description 1
• 102100025248 C-X-C motif chemokine 10 Human genes 0.000 description 1
• 101710098275 C-X-C motif chemokine 10 Proteins 0.000 description 1
• 102100036150 C-X-C motif chemokine 5 Human genes 0.000 description 1
• 102100036153 C-X-C motif chemokine 6 Human genes 0.000 description 1
• 101710085504 C-X-C motif chemokine 6 Proteins 0.000 description 1
• 102100036170 C-X-C motif chemokine 9 Human genes 0.000 description 1
• 101710085500 C-X-C motif chemokine 9 Proteins 0.000 description 1
• 102000001902 CC Chemokines Human genes 0.000 description 1
• 108010040471 CC Chemokines Proteins 0.000 description 1
• 101150013553 CD40 gene Proteins 0.000 description 1
• 102100032912 CD44 antigen Human genes 0.000 description 1
• 108050006947 CXC Chemokine Proteins 0.000 description 1
• 102000019388 CXC chemokine Human genes 0.000 description 1
• 101100392772 Caenorhabditis elegans gln-2 gene Proteins 0.000 description 1
• 102000055006 Calcitonin Human genes 0.000 description 1
• 108060001064 Calcitonin Proteins 0.000 description 1
• 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
• 239000004215 Carbon black (E152) Substances 0.000 description 1
• 108010055166 Chemokine CCL5 Proteins 0.000 description 1
• 108010055124 Chemokine CCL7 Proteins 0.000 description 1
• 102000000012 Chemokine CCL8 Human genes 0.000 description 1
• 108010055204 Chemokine CCL8 Proteins 0.000 description 1
• VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
• 241000223782 Ciliophora Species 0.000 description 1
• 102100022641 Coagulation factor IX Human genes 0.000 description 1
• 102100023804 Coagulation factor VII Human genes 0.000 description 1
• 108091026890 Coding region Proteins 0.000 description 1
• 102000008186 Collagen Human genes 0.000 description 1
• 108010035532 Collagen Proteins 0.000 description 1
• 229940124073 Complement inhibitor Drugs 0.000 description 1
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
• 241000711573 Coronaviridae Species 0.000 description 1
• OMFXVFTZEKFJBZ-UHFFFAOYSA-N Corticosterone Natural products O=C1CCC2(C)C3C(O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 OMFXVFTZEKFJBZ-UHFFFAOYSA-N 0.000 description 1
• 241000557626 Corvus corax Species 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 241000938605 Crocodylia Species 0.000 description 1
• JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
• QNAYBMKLOCPYGJ-UWTATZPHSA-N D-alanine Chemical compound C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
• QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
• ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
• 229930195713 D-glutamate Natural products 0.000 description 1
• YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
• 108010017826 DNA Polymerase I Proteins 0.000 description 1
• 102000004594 DNA Polymerase I Human genes 0.000 description 1
• 238000012270 DNA recombination Methods 0.000 description 1
• 230000004543 DNA replication Effects 0.000 description 1
• 241000450599 DNA viruses Species 0.000 description 1
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
• 102000016680 Dioxygenases Human genes 0.000 description 1
• 108010028143 Dioxygenases Proteins 0.000 description 1
• 241000224431 Entamoeba Species 0.000 description 1
• 101710104662 Enterotoxin type C-3 Proteins 0.000 description 1
• 102400001368 Epidermal growth factor Human genes 0.000 description 1
• 101800003838 Epidermal growth factor Proteins 0.000 description 1
• 108020002908 Epoxide hydrolase Proteins 0.000 description 1
• 102000005486 Epoxide hydrolase Human genes 0.000 description 1
• 241001125671 Eretmochelys imbricata Species 0.000 description 1
• 102000003951 Erythropoietin Human genes 0.000 description 1
• 102100031939 Erythropoietin Human genes 0.000 description 1
• 108090000394 Erythropoietin Proteins 0.000 description 1
• 101900234631 Escherichia coli DNA polymerase I Proteins 0.000 description 1
• 241000701988 Escherichia virus T5 Species 0.000 description 1
• 108090000371 Esterases Proteins 0.000 description 1
• 241000567412 Estigmene acrea Species 0.000 description 1
• 102100030844 Exocyst complex component 1 Human genes 0.000 description 1
• 108010076282 Factor IX Proteins 0.000 description 1
• 108010023321 Factor VII Proteins 0.000 description 1
• 108010054218 Factor VIII Proteins 0.000 description 1
• 102000001690 Factor VIII Human genes 0.000 description 1
• 108010014173 Factor X Proteins 0.000 description 1
• 108010049003 Fibrinogen Proteins 0.000 description 1
• 102000008946 Fibrinogen Human genes 0.000 description 1
• 108090000368 Fibroblast growth factor 8 Proteins 0.000 description 1
• 102100037362 Fibronectin Human genes 0.000 description 1
• 108010067306 Fibronectins Proteins 0.000 description 1
• 108090000331 Firefly luciferases Proteins 0.000 description 1
• 241000710831 Flavivirus Species 0.000 description 1
• 102000002464 Galactosidases Human genes 0.000 description 1
• 108010093031 Galactosidases Proteins 0.000 description 1
• 102100040837 Galactoside alpha-(1,2)-fucosyltransferase 2 Human genes 0.000 description 1
• 101710115997 Gamma-tubulin complex component 2 Proteins 0.000 description 1
• 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
• 241000224466 Giardia Species 0.000 description 1
• 102000004547 Glucosylceramidase Human genes 0.000 description 1
• 108010017544 Glucosylceramidase Proteins 0.000 description 1
• 102100024977 Glutamine-tRNA ligase Human genes 0.000 description 1
• 108010024636 Glutathione Proteins 0.000 description 1
• BCCRXDTUTZHDEU-VKHMYHEASA-N Gly-Ser Chemical compound NCC(=O)N[C@@H](CO)C(O)=O BCCRXDTUTZHDEU-VKHMYHEASA-N 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 102000003886 Glycoproteins Human genes 0.000 description 1
• 108090000288 Glycoproteins Proteins 0.000 description 1
• 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
• 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
• 108700023372 Glycosyltransferases Proteins 0.000 description 1
• 102000006771 Gonadotropins Human genes 0.000 description 1
• 108010086677 Gonadotropins Proteins 0.000 description 1
• 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
• 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
• 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
• 108010051696 Growth Hormone Proteins 0.000 description 1
• 102100034221 Growth-regulated alpha protein Human genes 0.000 description 1
• HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
• 101150009006 HIS3 gene Proteins 0.000 description 1
• 108090000031 Hedgehog Proteins Proteins 0.000 description 1
• 102000003693 Hedgehog Proteins Human genes 0.000 description 1
• 241000255967 Helicoverpa zea Species 0.000 description 1
• 241000256244 Heliothis virescens Species 0.000 description 1
• 101710154606 Hemagglutinin Proteins 0.000 description 1
• 102000001554 Hemoglobins Human genes 0.000 description 1
• 108010054147 Hemoglobins Proteins 0.000 description 1
• HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
• 102000007625 Hirudins Human genes 0.000 description 1
• 108010007267 Hirudins Proteins 0.000 description 1
• 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
• 101000947186 Homo sapiens C-X-C motif chemokine 5 Proteins 0.000 description 1
• 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 1
• 101000893710 Homo sapiens Galactoside alpha-(1,2)-fucosyltransferase 2 Proteins 0.000 description 1
• 101001069921 Homo sapiens Growth-regulated alpha protein Proteins 0.000 description 1
• 101000973997 Homo sapiens Nucleosome assembly protein 1-like 4 Proteins 0.000 description 1
• 101000947178 Homo sapiens Platelet basic protein Proteins 0.000 description 1
• 101000582950 Homo sapiens Platelet factor 4 Proteins 0.000 description 1
• 101001076715 Homo sapiens RNA-binding protein 39 Proteins 0.000 description 1
• 101000652229 Homo sapiens Suppressor of cytokine signaling 7 Proteins 0.000 description 1
• YZJSUQQZGCHHNQ-UHFFFAOYSA-N Homoglutamine Chemical compound OC(=O)C(N)CCCC(N)=O YZJSUQQZGCHHNQ-UHFFFAOYSA-N 0.000 description 1
• 102000002265 Human Growth Hormone Human genes 0.000 description 1
• 108010000521 Human Growth Hormone Proteins 0.000 description 1
• 239000000854 Human Growth Hormone Substances 0.000 description 1
• 102000008100 Human Serum Albumin Human genes 0.000 description 1
• 108091006905 Human Serum Albumin Proteins 0.000 description 1
• 241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
• 108060003951 Immunoglobulin Proteins 0.000 description 1
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
• 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
• 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
• 102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 1
• 108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 1
• 102100022339 Integrin alpha-L Human genes 0.000 description 1
• 108010008212 Integrin alpha4beta1 Proteins 0.000 description 1
• 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 description 1
• 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 1
• 108090000174 Interleukin-10 Proteins 0.000 description 1
• 108090000177 Interleukin-11 Proteins 0.000 description 1
• 108010065805 Interleukin-12 Proteins 0.000 description 1
• 108010002386 Interleukin-3 Proteins 0.000 description 1
• 108090000978 Interleukin-4 Proteins 0.000 description 1
• 108010002616 Interleukin-5 Proteins 0.000 description 1
• 108090001005 Interleukin-6 Proteins 0.000 description 1
• 108010002586 Interleukin-7 Proteins 0.000 description 1
• 108090001007 Interleukin-8 Proteins 0.000 description 1
• 108010002335 Interleukin-9 Proteins 0.000 description 1
• 108090000769 Isomerases Proteins 0.000 description 1
• 102000004195 Isomerases Human genes 0.000 description 1
• 229930194542 Keto Natural products 0.000 description 1
• 102100020880 Kit ligand Human genes 0.000 description 1
• 101710177504 Kit ligand Proteins 0.000 description 1
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
• 108010001831 LDL receptors Proteins 0.000 description 1
• 102000010445 Lactoferrin Human genes 0.000 description 1
• 108010063045 Lactoferrin Proteins 0.000 description 1
• 101710173438 Late L2 mu core protein Proteins 0.000 description 1
• 108090001090 Lectins Proteins 0.000 description 1
• 102000004856 Lectins Human genes 0.000 description 1
• 241000222722 Leishmania <genus> Species 0.000 description 1
• 102000004058 Leukemia inhibitory factor Human genes 0.000 description 1
• 108090000581 Leukemia inhibitory factor Proteins 0.000 description 1
• 108010054320 Lignin peroxidase Proteins 0.000 description 1
• 101710155614 Ligninase A Proteins 0.000 description 1
• 101710155621 Ligninase B Proteins 0.000 description 1
• 108090001060 Lipase Proteins 0.000 description 1
• 102000004882 Lipase Human genes 0.000 description 1
• 239000004367 Lipase Substances 0.000 description 1
• 102100024640 Low-density lipoprotein receptor Human genes 0.000 description 1
• 108060001084 Luciferase Proteins 0.000 description 1
• 239000005089 Luciferase Substances 0.000 description 1
• 108010064548 Lymphocyte Function-Associated Antigen-1 Proteins 0.000 description 1
• 102000004083 Lymphotoxin-alpha Human genes 0.000 description 1
• 108090000542 Lymphotoxin-alpha Proteins 0.000 description 1
• 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 1
• 241000555300 Mamestra Species 0.000 description 1
• 102100027754 Mast/stem cell growth factor receptor Kit Human genes 0.000 description 1
• 241000202974 Methanobacterium Species 0.000 description 1
• 241000205276 Methanosarcina Species 0.000 description 1
• 241000191938 Micrococcus luteus Species 0.000 description 1
• 241000243190 Microsporidia Species 0.000 description 1
• 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
• 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
• 101710154541 Modulator protein Proteins 0.000 description 1
• 241000699666 Mus <mouse, genus> Species 0.000 description 1
• 108010021466 Mutant Proteins Proteins 0.000 description 1
• 102000008300 Mutant Proteins Human genes 0.000 description 1
• 231100000678 Mycotoxin Toxicity 0.000 description 1
• NSTPXGARCQOSAU-VIFPVBQESA-N N-formyl-L-phenylalanine Chemical compound O=CN[C@H](C(=O)O)CC1=CC=CC=C1 NSTPXGARCQOSAU-VIFPVBQESA-N 0.000 description 1
• 108010015406 Neurturin Proteins 0.000 description 1
• 102000001839 Neurturin Human genes 0.000 description 1
• 108010033272 Nitrilase Proteins 0.000 description 1
• 108010024026 Nitrile hydratase Proteins 0.000 description 1
• 238000000636 Northern blotting Methods 0.000 description 1
• 101710163270 Nuclease Proteins 0.000 description 1
• GEYBMYRBIABFTA-VIFPVBQESA-N O-methyl-L-tyrosine Chemical compound COC1=CC=C(C[C@H](N)C(O)=O)C=C1 GEYBMYRBIABFTA-VIFPVBQESA-N 0.000 description 1
• 102000004140 Oncostatin M Human genes 0.000 description 1
• 108090000630 Oncostatin M Proteins 0.000 description 1
• 241000713112 Orthobunyavirus Species 0.000 description 1
• 241000702244 Orthoreovirus Species 0.000 description 1
• 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
• 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
• 102000004316 Oxidoreductases Human genes 0.000 description 1
• 108090000854 Oxidoreductases Proteins 0.000 description 1
• 108090000417 Oxygenases Proteins 0.000 description 1
• 102000004020 Oxygenases Human genes 0.000 description 1
• 108090000445 Parathyroid hormone Proteins 0.000 description 1
• 102000003982 Parathyroid hormone Human genes 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 102000035195 Peptidases Human genes 0.000 description 1
• 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
• 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
• 241000709664 Picornaviridae Species 0.000 description 1
• 108010064851 Plant Proteins Proteins 0.000 description 1
• 102100036154 Platelet basic protein Human genes 0.000 description 1
• 102100030304 Platelet factor 4 Human genes 0.000 description 1
• 208000000474 Poliomyelitis Diseases 0.000 description 1
• 239000002202 Polyethylene glycol Substances 0.000 description 1
• 101710146427 Probable tyrosine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
• 239000004365 Protease Substances 0.000 description 1
• 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
• 101710176177 Protein A56 Proteins 0.000 description 1
• 102000001253 Protein Kinase Human genes 0.000 description 1
• 101710188315 Protein X Proteins 0.000 description 1
• 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 description 1
• 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 description 1
• 208000003251 Pruritus Diseases 0.000 description 1
• 241000205226 Pyrobaculum Species 0.000 description 1
• ASNFTDCKZKHJSW-REOHCLBHSA-N Quisqualic acid Chemical class OC(=O)[C@@H](N)CN1OC(=O)NC1=O ASNFTDCKZKHJSW-REOHCLBHSA-N 0.000 description 1
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
• 102000004879 Racemases and epimerases Human genes 0.000 description 1
• 108090001066 Racemases and epimerases Proteins 0.000 description 1
• 108020004511 Recombinant DNA Proteins 0.000 description 1
• 102000003743 Relaxin Human genes 0.000 description 1
• 108090000103 Relaxin Proteins 0.000 description 1
• 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 1
• 102000002278 Ribosomal Proteins Human genes 0.000 description 1
• 108010000605 Ribosomal Proteins Proteins 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 102100023361 SAP domain-containing ribonucleoprotein Human genes 0.000 description 1
• 101710194492 SET-binding protein Proteins 0.000 description 1
• 206010040070 Septic Shock Diseases 0.000 description 1
• 108010056088 Somatostatin Proteins 0.000 description 1
• 102000005157 Somatostatin Human genes 0.000 description 1
• 102100038803 Somatotropin Human genes 0.000 description 1
• 238000002105 Southern blotting Methods 0.000 description 1
• 241000256251 Spodoptera frugiperda Species 0.000 description 1
• 241000295644 Staphylococcaceae Species 0.000 description 1
• 101000882406 Staphylococcus aureus Enterotoxin type C-1 Proteins 0.000 description 1
• 101000882403 Staphylococcus aureus Enterotoxin type C-2 Proteins 0.000 description 1
• 101001057112 Staphylococcus aureus Enterotoxin type D Proteins 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• 108010023197 Streptokinase Proteins 0.000 description 1
• 102100021669 Stromal cell-derived factor 1 Human genes 0.000 description 1
• 101710088580 Stromal cell-derived factor 1 Proteins 0.000 description 1
• 102000005158 Subtilisins Human genes 0.000 description 1
• 108010056079 Subtilisins Proteins 0.000 description 1
• 239000012505 Superdex™ Substances 0.000 description 1
• 102100030529 Suppressor of cytokine signaling 7 Human genes 0.000 description 1
• 241000589596 Thermus Species 0.000 description 1
• 108010022394 Threonine synthase Proteins 0.000 description 1
• 108010078233 Thymalfasin Proteins 0.000 description 1
• 102400000800 Thymosin alpha-1 Human genes 0.000 description 1
• 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
• 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
• 206010044248 Toxic shock syndrome Diseases 0.000 description 1
• 231100000650 Toxic shock syndrome Toxicity 0.000 description 1
• 101710120037 Toxin CcdB Proteins 0.000 description 1
• 102000003929 Transaminases Human genes 0.000 description 1
• 108090000340 Transaminases Proteins 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• 241000224526 Trichomonas Species 0.000 description 1
• 241000255993 Trichoplusia ni Species 0.000 description 1
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
• 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
• 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 1
• 102000018378 Tyrosine-tRNA ligase Human genes 0.000 description 1
• 101710107268 Tyrosine-tRNA ligase, mitochondrial Proteins 0.000 description 1
• 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
• 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
• 206010046865 Vaccinia virus infection Diseases 0.000 description 1
• 108010000134 Vascular Cell Adhesion Molecule-1 Proteins 0.000 description 1
• 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 1
• 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
• 102100023543 Vascular cell adhesion protein 1 Human genes 0.000 description 1
• 102100039037 Vascular endothelial growth factor A Human genes 0.000 description 1
• ZVNYJIZDIRKMBF-UHFFFAOYSA-N Vesnarinone Chemical compound C1=C(OC)C(OC)=CC=C1C(=O)N1CCN(C=2C=C3CCC(=O)NC3=CC=2)CC1 ZVNYJIZDIRKMBF-UHFFFAOYSA-N 0.000 description 1
• 229930003268 Vitamin C Natural products 0.000 description 1
• 108700040099 Xylose isomerases Proteins 0.000 description 1
• 238000009825 accumulation Methods 0.000 description 1
• 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
• 230000009471 action Effects 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 125000002252 acyl group Chemical group 0.000 description 1
• 238000001261 affinity purification Methods 0.000 description 1
• 229960002478 aldosterone Drugs 0.000 description 1
• 125000001931 aliphatic group Chemical group 0.000 description 1
• 125000003342 alkenyl group Chemical group 0.000 description 1
• 125000000217 alkyl group Chemical group 0.000 description 1
• 125000000304 alkynyl group Chemical group 0.000 description 1
• 102000015395 alpha 1-Antitrypsin Human genes 0.000 description 1
• 108010050122 alpha 1-Antitrypsin Proteins 0.000 description 1
• 229940024142 alpha 1-antitrypsin Drugs 0.000 description 1
• 150000001370 alpha-amino acid derivatives Chemical class 0.000 description 1
• 102000005922 amidase Human genes 0.000 description 1
• 150000001408 amides Chemical group 0.000 description 1
• 150000001412 amines Chemical class 0.000 description 1
• 229940093740 amino acid and derivative Drugs 0.000 description 1
• 229940124277 aminobutyric acid Drugs 0.000 description 1
• BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
• 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
• 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
• 235000011130 ammonium sulphate Nutrition 0.000 description 1
• 239000012491 analyte Substances 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 238000005571 anion exchange chromatography Methods 0.000 description 1
• 150000001450 anions Chemical class 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 230000002587 anti-hemolytic effect Effects 0.000 description 1
• 108010082685 antiarrhythmic peptide Proteins 0.000 description 1
• 239000000427 antigen Substances 0.000 description 1
• 108091007433 antigens Proteins 0.000 description 1
• 102000036639 antigens Human genes 0.000 description 1
• 239000003430 antimalarial agent Substances 0.000 description 1
• 229940033495 antimalarials Drugs 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• OZDNDGXASTWERN-UHFFFAOYSA-N apovincamine Natural products C1=CC=C2C(CCN3CCC4)=C5C3C4(CC)C=C(C(=O)OC)N5C2=C1 OZDNDGXASTWERN-UHFFFAOYSA-N 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• FZCSTZYAHCUGEM-UHFFFAOYSA-N aspergillomarasmine B Natural products OC(=O)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O FZCSTZYAHCUGEM-UHFFFAOYSA-N 0.000 description 1
• 230000001746 atrial effect Effects 0.000 description 1
• 244000052616 bacterial pathogen Species 0.000 description 1
• 238000013320 baculovirus expression vector system Methods 0.000 description 1
• 238000007630 basic procedure Methods 0.000 description 1
• 239000011324 bead Substances 0.000 description 1
• 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 239000011942 biocatalyst Substances 0.000 description 1
• 230000008827 biological function Effects 0.000 description 1
• 230000033228 biological regulation Effects 0.000 description 1
• 230000000853 biopesticidal effect Effects 0.000 description 1
• 229960002685 biotin Drugs 0.000 description 1
• 235000020958 biotin Nutrition 0.000 description 1
• 239000011616 biotin Substances 0.000 description 1
• LWISPDYGRSGXME-YDHLFZDLSA-N biotin peg2 amine Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCOCCOCCN)SC[C@@H]21 LWISPDYGRSGXME-YDHLFZDLSA-N 0.000 description 1
• ZADPBFCGQRWHPN-UHFFFAOYSA-N boronic acid Chemical compound OBO ZADPBFCGQRWHPN-UHFFFAOYSA-N 0.000 description 1
• 210000004899 c-terminal region Anatomy 0.000 description 1
• BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 description 1
• 229960004015 calcitonin Drugs 0.000 description 1
• 238000004364 calculation method Methods 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• 125000004432 carbon atom Chemical group C* 0.000 description 1
• NSQLIUXCMFBZME-MPVJKSABSA-N carperitide Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CSSC[C@@H](C(=O)N1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)=O)[C@@H](C)CC)C1=CC=CC=C1 NSQLIUXCMFBZME-MPVJKSABSA-N 0.000 description 1
• 238000005341 cation exchange Methods 0.000 description 1
• 230000024245 cell differentiation Effects 0.000 description 1
• 239000013592 cell lysate Substances 0.000 description 1
• 230000009134 cell regulation Effects 0.000 description 1
• 210000004671 cell-free system Anatomy 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 239000003638 chemical reducing agent Substances 0.000 description 1
• 229910052801 chlorine Inorganic materials 0.000 description 1
• 229960003677 chloroquine Drugs 0.000 description 1
• WHTVZRBIWZFKQO-UHFFFAOYSA-N chloroquine Natural products ClC1=CC=C2C(NC(C)CCCN(CC)CC)=CC=NC2=C1 WHTVZRBIWZFKQO-UHFFFAOYSA-N 0.000 description 1
• 239000013599 cloning vector Substances 0.000 description 1
• 229920001436 collagen Polymers 0.000 description 1
• 238000004040 coloring Methods 0.000 description 1
• 238000004440 column chromatography Methods 0.000 description 1
• 239000004074 complement inhibitor Substances 0.000 description 1
• 239000000470 constituent Substances 0.000 description 1
• 229910052802 copper Inorganic materials 0.000 description 1
• 239000010949 copper Substances 0.000 description 1
• OMFXVFTZEKFJBZ-HJTSIMOOSA-N corticosterone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@H](CC4)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 OMFXVFTZEKFJBZ-HJTSIMOOSA-N 0.000 description 1
• 125000004122 cyclic group Chemical group 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000006324 decarbonylation Effects 0.000 description 1
• 238000006606 decarbonylation reaction Methods 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• 230000000593 degrading effect Effects 0.000 description 1
• 238000009795 derivation Methods 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• 238000001784 detoxification Methods 0.000 description 1
• 229950006137 dexfosfoserine Drugs 0.000 description 1
• 238000010586 diagram Methods 0.000 description 1
• 238000009792 diffusion process Methods 0.000 description 1
• 102000004419 dihydrofolate reductase Human genes 0.000 description 1
• 239000002934 diuretic Substances 0.000 description 1
• 230000012361 double-strand break repair Effects 0.000 description 1
• 241001492478 dsDNA viruses, no RNA stage Species 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 238000000132 electrospray ionisation Methods 0.000 description 1
• 238000005516 engineering process Methods 0.000 description 1
• 108010048367 enhanced green fluorescent protein Proteins 0.000 description 1
• 231100000655 enterotoxin Toxicity 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 230000009483 enzymatic pathway Effects 0.000 description 1
• 229940116977 epidermal growth factor Drugs 0.000 description 1
• 229940105423 erythropoietin Drugs 0.000 description 1
• 150000002148 esters Chemical class 0.000 description 1
• 229940011871 estrogen Drugs 0.000 description 1
• 239000000262 estrogen Substances 0.000 description 1
• 238000012869 ethanol precipitation Methods 0.000 description 1
• 230000007717 exclusion Effects 0.000 description 1
• 239000002095 exotoxin Substances 0.000 description 1
• 231100000776 exotoxin Toxicity 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 229960004222 factor ix Drugs 0.000 description 1
• 229940012413 factor vii Drugs 0.000 description 1
• 229960000301 factor viii Drugs 0.000 description 1
• 229940012426 factor x Drugs 0.000 description 1
• 229940012952 fibrinogen Drugs 0.000 description 1
• ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Substances NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 1
• 238000013467 fragmentation Methods 0.000 description 1
• 238000006062 fragmentation reaction Methods 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 230000004927 fusion Effects 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 229960002743 glutamine Drugs 0.000 description 1
• 108010051239 glutaminyl-tRNA synthetase Proteins 0.000 description 1
• 229960003180 glutathione Drugs 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• 102000045442 glycosyltransferase activity proteins Human genes 0.000 description 1
• 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 1
• 229910052737 gold Inorganic materials 0.000 description 1
• 239000002622 gonadotropin Substances 0.000 description 1
• 125000005843 halogen group Chemical group 0.000 description 1
• 230000036541 health Effects 0.000 description 1
• 229960002897 heparin Drugs 0.000 description 1
• 229920000669 heparin Polymers 0.000 description 1
• 208000002672 hepatitis B Diseases 0.000 description 1
• 125000000623 heterocyclic group Chemical group 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 1
• 229940006607 hirudin Drugs 0.000 description 1
• 125000001165 hydrophobic group Chemical group 0.000 description 1
• 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
• 238000012872 hydroxylapatite chromatography Methods 0.000 description 1
• 238000003384 imaging method Methods 0.000 description 1
• 150000002466 imines Chemical class 0.000 description 1
• 150000007976 iminium ions Chemical class 0.000 description 1
• 230000003053 immunization Effects 0.000 description 1
• 238000002649 immunization Methods 0.000 description 1
• 238000003018 immunoassay Methods 0.000 description 1
• 230000002163 immunogen Effects 0.000 description 1
• 102000018358 immunoglobulin Human genes 0.000 description 1
• 229940072221 immunoglobulins Drugs 0.000 description 1
• 238000010324 immunological assay Methods 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 229940097275 indigo Drugs 0.000 description 1
• COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
• 230000002458 infectious effect Effects 0.000 description 1
• 230000002401 inhibitory effect Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 238000007689 inspection Methods 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 102000002467 interleukin receptors Human genes 0.000 description 1
• 108010093036 interleukin receptors Proteins 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 229910052741 iridium Inorganic materials 0.000 description 1
• JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
• YOBAEOGBNPPUQV-UHFFFAOYSA-N iron;trihydrate Chemical compound O.O.O.[Fe].[Fe] YOBAEOGBNPPUQV-UHFFFAOYSA-N 0.000 description 1
• 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
• 229930027917 kanamycin Natural products 0.000 description 1
• 229960000318 kanamycin Drugs 0.000 description 1
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
• 229930182823 kanamycin A Natural products 0.000 description 1
• CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
• 238000002372 labelling Methods 0.000 description 1
• 238000011005 laboratory method Methods 0.000 description 1
• 229940078795 lactoferrin Drugs 0.000 description 1
• 235000021242 lactoferrin Nutrition 0.000 description 1
• 229910052745 lead Inorganic materials 0.000 description 1
• 239000002523 lectin Substances 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 125000005647 linker group Chemical group 0.000 description 1
• 235000019421 lipase Nutrition 0.000 description 1
• 150000002632 lipids Chemical class 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 239000006194 liquid suspension Substances 0.000 description 1
• 101150109301 lys2 gene Proteins 0.000 description 1
• 239000012139 lysis buffer Substances 0.000 description 1
• 238000002844 melting Methods 0.000 description 1
• 230000008018 melting Effects 0.000 description 1
• 239000012528 membrane Substances 0.000 description 1
• 229910021645 metal ion Inorganic materials 0.000 description 1
• 150000002739 metals Chemical class 0.000 description 1
• 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
• 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 230000002906 microbiologic effect Effects 0.000 description 1
• 230000033607 mismatch repair Effects 0.000 description 1
• ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 239000000178 monomer Substances 0.000 description 1
• 230000000869 mutational effect Effects 0.000 description 1
• 239000002636 mycotoxin Substances 0.000 description 1
• 230000001452 natriuretic effect Effects 0.000 description 1
• 239000013642 negative control Substances 0.000 description 1
• 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
• 229910052757 nitrogen Inorganic materials 0.000 description 1
• 125000004433 nitrogen atom Chemical group N* 0.000 description 1
• 239000002777 nucleoside Substances 0.000 description 1
• 150000003833 nucleoside derivatives Chemical class 0.000 description 1
• 210000000056 organ Anatomy 0.000 description 1
• 239000003960 organic solvent Substances 0.000 description 1
• 230000002188 osteogenic effect Effects 0.000 description 1
• 230000003647 oxidation Effects 0.000 description 1
• 238000007254 oxidation reaction Methods 0.000 description 1
• PSWJVKKJYCAPTI-UHFFFAOYSA-N oxido-oxo-phosphonophosphanylphosphanium Chemical compound OP(O)(=O)PP(=O)=O PSWJVKKJYCAPTI-UHFFFAOYSA-N 0.000 description 1
• 125000004430 oxygen atom Chemical group O* 0.000 description 1
• 239000005022 packaging material Substances 0.000 description 1
• 238000012856 packing Methods 0.000 description 1
• 229910052763 palladium Inorganic materials 0.000 description 1
• 239000000199 parathyroid hormone Substances 0.000 description 1
• 229960001319 parathyroid hormone Drugs 0.000 description 1
• 238000003909 pattern recognition Methods 0.000 description 1
• 230000035515 penetration Effects 0.000 description 1
• 108010012038 peptide 78 Proteins 0.000 description 1
• 229940125863 peptide 78 Drugs 0.000 description 1
• 239000000813 peptide hormone Substances 0.000 description 1
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
• 239000000816 peptidomimetic Substances 0.000 description 1
• 230000035699 permeability Effects 0.000 description 1
• 238000002823 phage display Methods 0.000 description 1
• 150000002993 phenylalanine derivatives Chemical class 0.000 description 1
• 229940080469 phosphocellulose Drugs 0.000 description 1
• DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 description 1
• DCWXELXMIBXGTH-QMMMGPOBSA-N phosphonotyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-QMMMGPOBSA-N 0.000 description 1
• 229910052698 phosphorus Inorganic materials 0.000 description 1
• 230000000704 physical effect Effects 0.000 description 1
• 230000008635 plant growth Effects 0.000 description 1
• 229910052697 platinum Inorganic materials 0.000 description 1
• 102000005162 pleiotrophin Human genes 0.000 description 1
• 230000001402 polyadenylating effect Effects 0.000 description 1
• 229920001223 polyethylene glycol Polymers 0.000 description 1
• 229920000166 polytrimethylene carbonate Polymers 0.000 description 1
• 230000004481 post-translational protein modification Effects 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• 239000000047 product Substances 0.000 description 1
• 229960003387 progesterone Drugs 0.000 description 1
• 239000000186 progesterone Substances 0.000 description 1
• 108060006633 protein kinase Proteins 0.000 description 1
• 238000001814 protein method Methods 0.000 description 1
• 239000012460 protein solution Substances 0.000 description 1
• 230000001698 pyrogenic effect Effects 0.000 description 1
• 238000011002 quantification Methods 0.000 description 1
• 230000002285 radioactive effect Effects 0.000 description 1
• 230000001105 regulatory effect Effects 0.000 description 1
• 230000008439 repair process Effects 0.000 description 1
• 238000011160 research Methods 0.000 description 1
• 238000012827 research and development Methods 0.000 description 1
• 230000032537 response to toxin Effects 0.000 description 1
• 238000010839 reverse transcription Methods 0.000 description 1
• 238000012552 review Methods 0.000 description 1
• 210000003705 ribosome Anatomy 0.000 description 1
• 238000007363 ring formation reaction Methods 0.000 description 1
• 229920002477 rna polymer Polymers 0.000 description 1
• 201000005404 rubella Diseases 0.000 description 1
• 229920006395 saturated elastomer Polymers 0.000 description 1
• 229930195734 saturated hydrocarbon Natural products 0.000 description 1
• 238000012163 sequencing technique Methods 0.000 description 1
• 101150091813 shfl gene Proteins 0.000 description 1
• 239000013605 shuttle vector Substances 0.000 description 1
• 238000001542 size-exclusion chromatography Methods 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 239000011780 sodium chloride Substances 0.000 description 1
• 239000007787 solid Substances 0.000 description 1
• NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 description 1
• 229960000553 somatostatin Drugs 0.000 description 1
• 238000000527 sonication Methods 0.000 description 1
• 108010068698 spleen exonuclease Proteins 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 238000007619 statistical method Methods 0.000 description 1
• 108020003113 steroid hormone receptors Proteins 0.000 description 1
• 102000005969 steroid hormone receptors Human genes 0.000 description 1
• 230000004936 stimulating effect Effects 0.000 description 1
• 229960005202 streptokinase Drugs 0.000 description 1
• 125000003107 substituted aryl group Chemical group 0.000 description 1
• 235000000346 sugar Nutrition 0.000 description 1
• 231100000617 superantigen Toxicity 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000013589 supplement Substances 0.000 description 1
• 229960003604 testosterone Drugs 0.000 description 1
• 125000003396 thiol group Chemical group [H]S* 0.000 description 1
• 150000003573 thiols Chemical class 0.000 description 1
• RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
• NZVYCXVTEHPMHE-ZSUJOUNUSA-N thymalfasin Chemical compound CC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O NZVYCXVTEHPMHE-ZSUJOUNUSA-N 0.000 description 1
• 229960004231 thymalfasin Drugs 0.000 description 1
• 229940113082 thymine Drugs 0.000 description 1
• 229960000187 tissue plasminogen activator Drugs 0.000 description 1
• 238000006257 total synthesis reaction Methods 0.000 description 1
• 231100000419 toxicity Toxicity 0.000 description 1
• 230000001988 toxicity Effects 0.000 description 1
• 238000002723 toxicity assay Methods 0.000 description 1
• 230000005026 transcription initiation Effects 0.000 description 1
• 230000001131 transforming effect Effects 0.000 description 1
• 230000009261 transgenic effect Effects 0.000 description 1
• 230000014621 translational initiation Effects 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• 235000011178 triphosphate Nutrition 0.000 description 1
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
• 125000005454 tryptophanyl group Chemical group 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 229930195735 unsaturated hydrocarbon Natural products 0.000 description 1
• 229960005356 urokinase Drugs 0.000 description 1
• 208000007089 vaccinia Diseases 0.000 description 1
• 238000012795 verification Methods 0.000 description 1
• 239000013603 viral vector Substances 0.000 description 1
• 230000003612 virological effect Effects 0.000 description 1
• 235000019154 vitamin C Nutrition 0.000 description 1
• 239000011718 vitamin C Substances 0.000 description 1
• 238000012982 x-ray structure analysis Methods 0.000 description 1
• 210000005253 yeast cell Anatomy 0.000 description 1
• JPZXHKDZASGCLU-LBPRGKRZSA-N β-(2-naphthyl)-alanine Chemical compound C1=CC=CC2=CC(C[C@H](N)C(O)=O)=CC=C21 JPZXHKDZASGCLU-LBPRGKRZSA-N 0.000 description 1