MXPA02005089A - Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs). - Google Patents

Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs).

Info

Publication number
MXPA02005089A
MXPA02005089A MXPA02005089A MXPA02005089A MXPA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A
Authority
MX
Mexico
Prior art keywords
protein
plant
gfp
suppressor
nucleic acid
Prior art date
Application number
MXPA02005089A
Other languages
English (en)
Spanish (es)
Inventor
David Charles Baulcombe
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Publication of MXPA02005089A publication Critical patent/MXPA02005089A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
MXPA02005089A 1999-11-22 2000-11-22 Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs). MXPA02005089A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
PCT/GB2000/004454 WO2001038512A2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Publications (1)

Publication Number Publication Date
MXPA02005089A true MXPA02005089A (es) 2003-01-28

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02005089A MXPA02005089A (es) 1999-11-22 2000-11-22 Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs).

Country Status (12)

Country Link
US (2) US7217854B1 (https=)
EP (1) EP1232274B1 (https=)
JP (1) JP4884628B2 (https=)
KR (1) KR20030004305A (https=)
AT (1) ATE460490T1 (https=)
AU (1) AU782788B2 (https=)
CA (1) CA2390152C (https=)
DE (1) DE60043992D1 (https=)
IL (1) IL149388A0 (https=)
MX (1) MXPA02005089A (https=)
NZ (1) NZ518947A (https=)
WO (1) WO2001038512A2 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
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WO2001034822A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Control of post-transcriptional gene silencing in plants
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
EP1594956A4 (en) 2003-02-03 2007-08-01 Fraunhofer Usa Inc SYSTEM FOR EXPRESSION OF GENES IN PLANTS
EP1672066B1 (en) * 2003-10-01 2012-01-18 Japan Science and Technology Agency Dna fragment, method for producing transformant for protein production and utilization thereof
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
AU2005216133A1 (en) * 2004-02-20 2005-09-09 Fraunhofer Usa, Inc. Systems and methods for clonal expression in plants
EP2357244A3 (en) 2004-04-22 2011-11-23 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) * 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
WO2007092460A2 (en) * 2006-02-03 2007-08-16 The Texas A & M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP1897953A1 (en) * 2006-09-06 2008-03-12 Icon Genetics GmbH Potexvirus-derived replicon
US9605270B2 (en) * 2006-09-22 2017-03-28 The Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
WO2008094512A2 (en) * 2007-01-29 2008-08-07 The Ohio Sate University Research Foundation System for expression of genes in plants from a virus-based expression vector
WO2008138896A1 (en) * 2007-05-09 2008-11-20 Devgen N.V. A method for sustainable transgene transcription
JP5785080B2 (ja) * 2008-08-27 2015-09-24 アリゾナ・ボード・オブ・リージェンツ・フォー・アンド・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティArizona Board Of Regents For And On Behalf Of Arizona State University 植物におけるワクチン及びモノクローナル抗体治療薬の高レベル迅速生産のためのdnaレプリコン系
AU2013204254B8 (en) * 2008-11-18 2015-10-08 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
BRPI0921467B1 (pt) 2008-11-18 2021-12-14 Commonwealth Scientific And Industrial Research Organisation Processo para a produção de óleo compreendendo ácido eicosapentaenóico (epa), ácido docosapentaenóico (dpa) e ácido docosahexaenóico (dha)
WO2010099462A2 (en) * 2009-02-26 2010-09-02 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) * 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
CA2824155A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
KR20140034139A (ko) 2011-01-17 2014-03-19 필립모리스 프로덕츠 에스.에이. 식물에서의 단백질 발현
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ns3유전자의 신규 용도
ES2744240T3 (es) * 2011-12-27 2020-02-24 Commw Scient Ind Res Org Silenciamiento génico y supresión del silenciamiento génico simultáneos en la misma célula
WO2013185184A2 (en) 2012-06-15 2013-12-19 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
EP3082405A4 (en) 2013-12-18 2017-12-13 Commonwealth Scientific and Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
SG11201610596PA (en) 2014-06-27 2017-01-27 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
EP3682012A1 (en) 2017-09-11 2020-07-22 R. J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
EP3880828A4 (en) * 2018-11-13 2022-08-03 University of Maryland, College Park PLANT VECTORS, COMPOSITIONS AND USES RELATED THERETO
CN115052979A (zh) 2019-11-12 2022-09-13 马里兰大学派克分院 植物载体、组合物及其相关用途
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用
CN119410670A (zh) * 2025-01-06 2025-02-11 中国热带农业科学院三亚研究院 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
WO2001034822A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Control of post-transcriptional gene silencing in plants

Also Published As

Publication number Publication date
US8871997B2 (en) 2014-10-28
KR20030004305A (ko) 2003-01-14
EP1232274B1 (en) 2010-03-10
NZ518947A (en) 2004-05-28
WO2001038512A2 (en) 2001-05-31
US7217854B1 (en) 2007-05-15
JP4884628B2 (ja) 2012-02-29
AU782788B2 (en) 2005-08-25
DE60043992D1 (de) 2010-04-22
JP2003514585A (ja) 2003-04-22
CA2390152C (en) 2012-08-28
EP1232274A2 (en) 2002-08-21
IL149388A0 (en) 2002-11-10
ATE460490T1 (de) 2010-03-15
WO2001038512A3 (en) 2002-03-28
AU1533401A (en) 2001-06-04
CA2390152A1 (en) 2001-05-31
US20070292862A1 (en) 2007-12-20

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