CA2390152C - Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) - Google Patents

Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) Download PDF

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Publication number
CA2390152C
CA2390152C CA2390152A CA2390152A CA2390152C CA 2390152 C CA2390152 C CA 2390152C CA 2390152 A CA2390152 A CA 2390152A CA 2390152 A CA2390152 A CA 2390152A CA 2390152 C CA2390152 C CA 2390152C
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CA
Canada
Prior art keywords
protein
plant
gfp
nucleic acid
suppressor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2390152A
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English (en)
French (fr)
Other versions
CA2390152A1 (en
Inventor
David Charles Baulcombe
Olivier Voinnet
Andrew John Hamilton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Bioscience Ltd
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Plant Bioscience Ltd
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Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Publication of CA2390152A1 publication Critical patent/CA2390152A1/en
Application granted granted Critical
Publication of CA2390152C publication Critical patent/CA2390152C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CA2390152A 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) Expired - Lifetime CA2390152C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB9927609.9 1999-11-22
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB0022960.9 2000-09-19
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0023668.7 2000-09-27
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
PCT/GB2000/004454 WO2001038512A2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Publications (2)

Publication Number Publication Date
CA2390152A1 CA2390152A1 (en) 2001-05-31
CA2390152C true CA2390152C (en) 2012-08-28

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2390152A Expired - Lifetime CA2390152C (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Country Status (12)

Country Link
US (2) US7217854B1 (https=)
EP (1) EP1232274B1 (https=)
JP (1) JP4884628B2 (https=)
KR (1) KR20030004305A (https=)
AT (1) ATE460490T1 (https=)
AU (1) AU782788B2 (https=)
CA (1) CA2390152C (https=)
DE (1) DE60043992D1 (https=)
IL (1) IL149388A0 (https=)
MX (1) MXPA02005089A (https=)
NZ (1) NZ518947A (https=)
WO (1) WO2001038512A2 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
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WO2001034822A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Control of post-transcriptional gene silencing in plants
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
EP1594956A4 (en) 2003-02-03 2007-08-01 Fraunhofer Usa Inc SYSTEM FOR EXPRESSION OF GENES IN PLANTS
EP1672066B1 (en) * 2003-10-01 2012-01-18 Japan Science and Technology Agency Dna fragment, method for producing transformant for protein production and utilization thereof
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
AU2005216133A1 (en) * 2004-02-20 2005-09-09 Fraunhofer Usa, Inc. Systems and methods for clonal expression in plants
EP2357244A3 (en) 2004-04-22 2011-11-23 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
CN103451246B (zh) 2004-04-22 2018-02-16 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) * 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
WO2007092460A2 (en) * 2006-02-03 2007-08-16 The Texas A & M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP1897953A1 (en) * 2006-09-06 2008-03-12 Icon Genetics GmbH Potexvirus-derived replicon
US9605270B2 (en) * 2006-09-22 2017-03-28 The Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
WO2008094512A2 (en) * 2007-01-29 2008-08-07 The Ohio Sate University Research Foundation System for expression of genes in plants from a virus-based expression vector
WO2008138896A1 (en) * 2007-05-09 2008-11-20 Devgen N.V. A method for sustainable transgene transcription
JP5785080B2 (ja) * 2008-08-27 2015-09-24 アリゾナ・ボード・オブ・リージェンツ・フォー・アンド・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティArizona Board Of Regents For And On Behalf Of Arizona State University 植物におけるワクチン及びモノクローナル抗体治療薬の高レベル迅速生産のためのdnaレプリコン系
AU2013204254B8 (en) * 2008-11-18 2015-10-08 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
BRPI0921467B1 (pt) 2008-11-18 2021-12-14 Commonwealth Scientific And Industrial Research Organisation Processo para a produção de óleo compreendendo ácido eicosapentaenóico (epa), ácido docosapentaenóico (dpa) e ácido docosahexaenóico (dha)
WO2010099462A2 (en) * 2009-02-26 2010-09-02 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) * 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
CA2824155A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
KR20140034139A (ko) 2011-01-17 2014-03-19 필립모리스 프로덕츠 에스.에이. 식물에서의 단백질 발현
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ns3유전자의 신규 용도
ES2744240T3 (es) * 2011-12-27 2020-02-24 Commw Scient Ind Res Org Silenciamiento génico y supresión del silenciamiento génico simultáneos en la misma célula
WO2013185184A2 (en) 2012-06-15 2013-12-19 Commonwealth Scientific And Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
EP3082405A4 (en) 2013-12-18 2017-12-13 Commonwealth Scientific and Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
SG11201610596PA (en) 2014-06-27 2017-01-27 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
EP3682012A1 (en) 2017-09-11 2020-07-22 R. J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
EP3880828A4 (en) * 2018-11-13 2022-08-03 University of Maryland, College Park PLANT VECTORS, COMPOSITIONS AND USES RELATED THERETO
CN115052979A (zh) 2019-11-12 2022-09-13 马里兰大学派克分院 植物载体、组合物及其相关用途
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用
CN119410670A (zh) * 2025-01-06 2025-02-11 中国热带农业科学院三亚研究院 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
WO2001034822A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Control of post-transcriptional gene silencing in plants

Also Published As

Publication number Publication date
US8871997B2 (en) 2014-10-28
KR20030004305A (ko) 2003-01-14
EP1232274B1 (en) 2010-03-10
NZ518947A (en) 2004-05-28
WO2001038512A2 (en) 2001-05-31
US7217854B1 (en) 2007-05-15
JP4884628B2 (ja) 2012-02-29
AU782788B2 (en) 2005-08-25
DE60043992D1 (de) 2010-04-22
MXPA02005089A (es) 2003-01-28
JP2003514585A (ja) 2003-04-22
EP1232274A2 (en) 2002-08-21
IL149388A0 (en) 2002-11-10
ATE460490T1 (de) 2010-03-15
WO2001038512A3 (en) 2002-03-28
AU1533401A (en) 2001-06-04
CA2390152A1 (en) 2001-05-31
US20070292862A1 (en) 2007-12-20

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