MX9709317A - Cromosomas sinteticos de mamifero y metodos para su construccion. - Google Patents

Cromosomas sinteticos de mamifero y metodos para su construccion.

Info

Publication number
MX9709317A
MX9709317A MX9709317A MX9709317A MX9709317A MX 9709317 A MX9709317 A MX 9709317A MX 9709317 A MX9709317 A MX 9709317A MX 9709317 A MX9709317 A MX 9709317A MX 9709317 A MX9709317 A MX 9709317A
Authority
MX
Mexico
Prior art keywords
dna
gene therapy
synthetic
chromosome
construction
Prior art date
Application number
MX9709317A
Other languages
English (en)
Inventor
Gil B Van Bokkelen
John J Harrington
Huntington F Willard
Original Assignee
Univ Case Western Reserve
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/487,989 external-priority patent/US5695967A/en
Application filed by Univ Case Western Reserve filed Critical Univ Case Western Reserve
Publication of MX9709317A publication Critical patent/MX9709317A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/208—Pseudochromosomes, minichrosomosomes of mammalian origin, e.g. minichromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La invencion se refiere al campo de la terapia génica, a la expresion génica y a los vectores para esto usos. En particular, la invencion se refiere a un método para producir unidades de ADN repetitivas grandes, estructurales intactas, especialmente utiles para la clonacion estable del ADN satélite alfa, y para el desarrollo y el uso de un cromosomas sintético o artificial para la expresion génica y la terapia génica en mamíferos, y especialmente en humanos. La invencion permite la construccion controlada de los cromosomas sintéticos o artificiales estables construidos a partir de los segmentos aislados de ADN purificado. Los segmentos mínimos funcionales incluyen preferentemente ADN centromérico, ADN telomérico, y ADN genomico. El cromosoma artificial realiza las funciones cromosomicas esenciales de los cromosomas de origen natural, para permitir que el cromosomas funcione como un vector efectivo para la terapia génica.
MX9709317A 1995-06-07 1997-12-01 Cromosomas sinteticos de mamifero y metodos para su construccion. MX9709317A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/487,989 US5695967A (en) 1995-06-07 1995-06-07 Method for stably cloning large repeating units of DNA
US08/643,554 US6348353B1 (en) 1995-06-07 1996-05-06 Artificial mammalian chromosome

Publications (1)

Publication Number Publication Date
MX9709317A true MX9709317A (es) 1998-08-30

Family

ID=27049198

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9709317A MX9709317A (es) 1995-06-07 1997-12-01 Cromosomas sinteticos de mamifero y metodos para su construccion.

Country Status (12)

Country Link
US (3) US6348353B1 (es)
EP (1) EP0832273A1 (es)
JP (1) JPH11507540A (es)
CN (1) CN1191574A (es)
AU (1) AU724695B2 (es)
BR (1) BR9608586A (es)
CA (1) CA2224114A1 (es)
HU (1) HUP9901072A2 (es)
IL (1) IL122414A0 (es)
MX (1) MX9709317A (es)
NZ (1) NZ311448A (es)
WO (1) WO1996040965A1 (es)

Families Citing this family (31)

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Publication number Priority date Publication date Assignee Title
US5869294A (en) * 1995-06-07 1999-02-09 Case Western Reserve University Method for stably cloning large repeating DNA sequences
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US6632976B1 (en) 1995-08-29 2003-10-14 Kirin Beer Kabushiki Kaisha Chimeric mice that are produced by microcell mediated chromosome transfer and that retain a human antibody gene
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6297029B1 (en) 1996-08-26 2001-10-02 Tsuneko Okazaki Mammalian artificial chromosomes
WO1998051790A1 (en) 1997-05-13 1998-11-19 Amrad Operations Pty. Ltd. A novel nucleic acid molecule
DE19720839B4 (de) * 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US6090584A (en) * 1997-08-21 2000-07-18 University Technologies International Inc. Baculovirus artificial chromosomes and methods of use
WO2000049142A1 (de) * 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
KR100863823B1 (ko) * 1999-12-22 2008-10-15 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 코스미드 ｄｎａ 작제물 및 이를 제조하고 이용하는 방법
JP4115281B2 (ja) * 2001-05-11 2008-07-09 キリンファーマ株式会社 ヒト抗体λ軽鎖遺伝子を含むヒト人工染色体、および子孫伝達可能な該ヒト人工染色体を含む非ヒト動物
US20040210955A1 (en) * 2001-10-26 2004-10-21 Hidenori Akutsu Preparation of spermatozoa for ICSI-mediated transgenesis and methods of using the same
AU2002953516A0 (en) * 2002-12-23 2003-01-16 Murdoch Childrens Research Institute Genetic therapy and genetic modification
BR0318377A (pt) * 2003-06-27 2006-07-25 Chromatin Inc composições de centrÈmero de planta
WO2005014786A2 (en) * 2003-08-07 2005-02-17 Athersys, Inc. Enhanced engineered chromosome formation from alpha satellite with artificially increased density of cenp-b boxes
CA2557644C (en) * 2004-02-23 2016-05-24 Chromatin, Inc. Plants modified with mini-chromosomes
EP2527456B1 (en) 2004-10-22 2018-05-16 Revivicor Inc. Transgenic porcines lacking endogenous immunoglobulin light chain
AU2006287553A1 (en) * 2005-09-08 2007-03-15 Chromatin, Inc. Plants modified with mini-chromosomes
WO2008112972A2 (en) * 2007-03-15 2008-09-18 Chromatin, Inc. Centromere sequences and minichromosomes
EP2207899B1 (en) * 2007-10-08 2018-04-18 Synthetic Genomics, Inc. Assembly of large nucleic acids
EP2348827B1 (en) 2008-10-27 2015-07-01 Revivicor, Inc. Immunocompromised ungulates
AU2010275448B2 (en) 2009-07-23 2014-03-06 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
WO2016130568A1 (en) * 2015-02-09 2016-08-18 Edward Perkins Compositions and methods for monitoring in real-time construction and bioengineering of mammalian synthetic chromosomes
CN106318965B (zh) * 2015-06-26 2019-05-07 深圳华大生命科学研究院 人工半合成染色体的整合方法及含有完整合成染色体的微生物

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US5049504A (en) 1986-11-24 1991-09-17 Genex Corporation Bioadhesive coding sequences
US5202256A (en) 1984-09-13 1993-04-13 Enzon Labs, Inc. Bioadhesive precursor protein expression vectors
US5242808A (en) 1984-09-13 1993-09-07 Enzon, Inc. Production of bioadhesive precursor protein analogs by genetically engineered organisms
US5149657A (en) 1984-09-13 1992-09-22 Enzon Labs Inc. Escherichia coli expression vector encoding bioadhesive precursor protein analogs comprising three to twenty repeats of the decapeptide (Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys)
US5202236A (en) 1984-09-13 1993-04-13 Enzon Labs Inc. Method of producing bioadhesive protein
FR2593827B1 (fr) 1986-01-31 1989-12-22 Inst Nat Sante Rech Med Fragments d'adn utilisables dans des vecteurs pour le maintien hereditaire de genes etrangers a l'etat autonome dans des animaux transgeniques, procede pour leur obtention et applications biologiques
US4889806A (en) * 1987-04-15 1989-12-26 Washington University Large DNA cloning system based on yeast artificial chromosomes
US4889906A (en) * 1988-11-25 1989-12-26 Monsanto Company Amine salts of 1,4,2-oxazaphospholidine-4-acetic acid, 2-alkoxy-2-oxides
WO1991007496A1 (en) 1989-11-09 1991-05-30 Allied-Signal Inc. Cloning and expression of genes encoding for polypeptides comprised of one or more repeating amino acid sequences
AU8904691A (en) 1990-10-17 1992-05-20 Board Of Regents, The University Of Texas System A eukaryotic episomal dna cloning and expression vector
US5288625A (en) 1991-09-13 1994-02-22 Biologic Research Center Of The Hungarian Academy Of Sciences Mammalian artificial chromosomes
GB9410446D0 (en) * 1994-05-25 1994-07-13 Cancer Res Campaign Tech Novel chromosome fragment
US5695967A (en) 1995-06-07 1997-12-09 Case Western Reserve University Method for stably cloning large repeating units of DNA
US5869294A (en) 1995-06-07 1999-02-09 Case Western Reserve University Method for stably cloning large repeating DNA sequences
US6348353B1 (en) 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes

Also Published As

Publication number Publication date
BR9608586A (pt) 1998-12-29
EP0832273A1 (en) 1998-04-01
IL122414A0 (en) 1998-06-15
AU724695B2 (en) 2000-09-28
CA2224114A1 (en) 1996-12-19
HUP9901072A2 (hu) 1999-07-28
US20050196837A1 (en) 2005-09-08
US20030003466A1 (en) 2003-01-02
CN1191574A (zh) 1998-08-26
NZ311448A (en) 2004-12-24
US6348353B1 (en) 2002-02-19
JPH11507540A (ja) 1999-07-06
AU6278196A (en) 1996-12-30
WO1996040965A1 (en) 1996-12-19

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Legal Events

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FG Grant or registration
MM Annulment or lapse due to non-payment of fees