MX9709308A - Mutantes de proteus vulgaris productores de condroitinasa i y condroitinasa ii. - Google Patents

Mutantes de proteus vulgaris productores de condroitinasa i y condroitinasa ii.

Info

Publication number
MX9709308A
MX9709308A MX9709308A MX9709308A MX9709308A MX 9709308 A MX9709308 A MX 9709308A MX 9709308 A MX9709308 A MX 9709308A MX 9709308 A MX9709308 A MX 9709308A MX 9709308 A MX9709308 A MX 9709308A
Authority
MX
Mexico
Prior art keywords
chondroitinase
vulgaris
cells
mutants
proteins
Prior art date
Application number
MX9709308A
Other languages
English (en)
Other versions
MXPA97009308A (es
Inventor
Jason Arnold Lotvin
Kiran Manohar Khandke
Mark Edward Ruppen
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of MXPA97009308A publication Critical patent/MXPA97009308A/es
Publication of MX9709308A publication Critical patent/MX9709308A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/37—Proteus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporcionan capas mutantes de Proteus vulgaris que, cuando se hacen crecer en ausencia de un inductor exogeno de condroitinasa I y II, producen proteínas condroitinasa I y condroitinasa II de P. vulgaris. Los mutantes típicamente producen proteínas condroitinasa I y II en ausencia de inductores exogenos en cantidades que exceden a las producidas por cepas de tipo silvestre de P. vulgaris, con tales inductores. Se describen dos clases de tales mutantes, clase I y II. Los mutantes clase I y clase II difieren en las cantidades relativas de condroitinasa I y II producidas cuando las células se hacen crecer en medio mínimo suplementario con casaaminoácidos. También se proporcionan variantes genotípicas adicionales que liberan proteínas condroitinasa I en el medio de cultivo. También se considera un método para producir proteínas condroitinasa I y II de P. vulgaris. Las células mutantes descritas en lo anterior se cultivan en ausencia de inductor exogeno convencional para condroitinasa I y II, después de lo cual las células se recolectan y se recuperan condroitinasa I y II a partir de las células recolectadas. También se proporciona un método para deteccion in situ de produccion de condroitinasa I y II por colonias bacterianas.
MX9709308A 1995-06-07 1996-06-04 Mutantes de proteus vulgaris productores de condroitinasa i y condroitinasa ii. MX9709308A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/476,261 US5888798A (en) 1995-06-07 1995-06-07 Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
US08476261 1995-06-07
PCT/US1996/008593 WO1996040897A2 (en) 1995-06-07 1996-06-04 Chondroitinase i and chondroitinase ii producing mutants of p. vulgaris

Publications (2)

Publication Number Publication Date
MXPA97009308A MXPA97009308A (es) 1998-02-01
MX9709308A true MX9709308A (es) 1998-02-28

Family

ID=23891149

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9709308A MX9709308A (es) 1995-06-07 1996-06-04 Mutantes de proteus vulgaris productores de condroitinasa i y condroitinasa ii.

Country Status (7)

Country Link
US (1) US5888798A (es)
EP (1) EP0832206A2 (es)
JP (1) JPH11506929A (es)
AU (1) AU6035596A (es)
CA (1) CA2221193A1 (es)
MX (1) MX9709308A (es)
WO (1) WO1996040897A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1737954A2 (en) * 2004-03-10 2007-01-03 The Massachusetts Institute Of Technology Recombinant chondroitinase abc i and uses thereof
US9796970B1 (en) 2017-04-24 2017-10-24 Advantek Serum Laboratories Ltd. Production of high purity chondroitinase ABC

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1067253A (en) * 1965-02-15 1967-05-03 Biorex Laboratories Ltd Process for the preparation of enzymes
US4696816A (en) * 1985-11-07 1987-09-29 Brown Mark D Method for treating intervertebral disc displacement with enzymes
JPS62122588A (ja) * 1985-11-22 1987-06-03 Taiyo Fishery Co Ltd 純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JPH0822223B2 (ja) * 1988-12-19 1996-03-06 東洋紡績株式会社 ＰｖｕＩ制限エンドヌクレアーゼの製造方法
ZA912770B (en) * 1990-04-16 1992-01-29 Bethesda Eye Inst Enzymatic disinsertion of vitreous body
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
JP3419811B2 (ja) * 1993-02-24 2003-06-23 マルハ株式会社 コンドロイチナーゼ遺伝子
JPH0698769A (ja) * 1992-09-22 1994-04-12 Maruha Corp コンドロイチナーゼ及びその遺伝子
WO1994025567A1 (en) * 1993-04-23 1994-11-10 American Cyanamid Company CLONING AND EXPRESSION OF THE CHONDROITINASE I AND II GENES FROM $i(P. VULGARIS)
US5525500A (en) * 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris

Also Published As

Publication number Publication date
JPH11506929A (ja) 1999-06-22
US5888798A (en) 1999-03-30
AU6035596A (en) 1996-12-30
WO1996040897A3 (en) 1997-04-24
WO1996040897A2 (en) 1996-12-19
EP0832206A2 (en) 1998-04-01
CA2221193A1 (en) 1996-12-19

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