MX9707635A - Inmunoanalisis sensibles que utilizan conjugados anticuerpos con moldes de adn replicables. - Google Patents

Inmunoanalisis sensibles que utilizan conjugados anticuerpos con moldes de adn replicables.

Info

Publication number
MX9707635A
MX9707635A MX9707635A MX9707635A MX9707635A MX 9707635 A MX9707635 A MX 9707635A MX 9707635 A MX9707635 A MX 9707635A MX 9707635 A MX9707635 A MX 9707635A MX 9707635 A MX9707635 A MX 9707635A
Authority
MX
Mexico
Prior art keywords
detect
rna
analyte
variant
antibody conjugates
Prior art date
Application number
MX9707635A
Other languages
English (en)
Inventor
Richard A Martinelli
Eddie Carroll Iii
Original Assignee
Chiron Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Diagnostics Corp filed Critical Chiron Diagnostics Corp
Publication of MX9707635A publication Critical patent/MX9707635A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Se describe un sistema de amplificacion de señales novedoso para inmunoanálisis que reducen al mínimo las señales no específicas. Los métodos de inmunoanálisis, reactivos y equipos de pruebas se describen para obtener inmunoanálisis de sensibilidad incrementada. Los reactivos incluyen conjugados de anticuerpos-ADN variantes, en donde el ADN variante es un substrato para una polimerasa de ARN dependientes de ARN, tales como replicasa de QB. Los métodos de inmunoanálisis para detectar, o para detectar y cuantificar el analito en muestras de prueba, comprenden transcribir el ADN variante de dichos conjugados de anticuerpos-ADN que se unen al analito, a ARN y replican las transcripciones de ARN, en donde la presencia o cantidad de replicacion de productos de replicacion de ARN variante se puede correlacionar con la presencia o cantidad de analito en las muestras de prueba. Además, se proveen métodos para detectar, o para detectar y cuantificar, simultáneamente dos o más analitos en muestra de prueba.
MX9707635A 1995-04-04 1996-04-01 Inmunoanalisis sensibles que utilizan conjugados anticuerpos con moldes de adn replicables. MX9707635A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/416,571 US6083689A (en) 1990-10-16 1995-04-04 Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates
PCT/US1996/004494 WO1996032640A1 (en) 1995-04-04 1996-04-01 Sensitive immunoassays utilizing antibody conjugates with replicable dna templates

Publications (1)

Publication Number Publication Date
MX9707635A true MX9707635A (es) 1997-12-31

Family

ID=23650484

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9707635A MX9707635A (es) 1995-04-04 1996-04-01 Inmunoanalisis sensibles que utilizan conjugados anticuerpos con moldes de adn replicables.

Country Status (12)

Country Link
US (1) US6083689A (es)
EP (1) EP0819249B1 (es)
JP (1) JPH11503828A (es)
KR (1) KR19980703574A (es)
AT (1) ATE206520T1 (es)
AU (1) AU5850396A (es)
BR (1) BR9604965A (es)
CA (1) CA2216466A1 (es)
DE (1) DE69615673D1 (es)
MX (1) MX9707635A (es)
PL (1) PL322650A1 (es)
WO (1) WO1996032640A1 (es)

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DE19959857C1 (de) * 1999-12-10 2001-06-28 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis von Analyten sowie ein Verfahren zur Herstellung und Verwendung
WO2001061349A1 (en) * 2000-02-18 2001-08-23 The University Of Tennessee Research Corporation Detection of microbial deposition and immune response at the basement membrane
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
DE10041766A1 (de) * 2000-08-25 2002-03-14 Friz Biochem Gmbh Verfahren zur Markierung chemischer Substanzen
GB0029617D0 (en) * 2000-12-05 2001-01-17 Norchip As Ligand detection method
CA2430329A1 (en) * 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
ATE361996T1 (de) * 2001-03-09 2007-06-15 Nugen Technologies Inc Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
US7018795B2 (en) * 2001-03-23 2006-03-28 Fuji Photo Film Co., Ltd. Hybridization probe and target nucleic acid detecting kit, target nucleic acid detecting apparatus and target nucleic acid detecting method using the same
US7077982B2 (en) * 2001-03-23 2006-07-18 Fuji Photo Film Co., Ltd. Molecular electric wire, molecular electric wire circuit using the same and process for producing the molecular electric wire circuit
US20020168291A1 (en) * 2001-03-23 2002-11-14 Fuji Photo Film Co., Ltd. Agent for health inspection and health inspection apparatus using the same
US20020168667A1 (en) * 2001-03-23 2002-11-14 Fuji Photo Film Co., Ltd. Antigen detecting agent and antigen detecting kit, antigen detecting apparatus and antigen detecting method using the same
US20030003476A1 (en) * 2001-03-23 2003-01-02 Fuji Photo Film Co., Ltd. Waste water inspecting agent and waste water inspecting apparatus using the same
US20020168756A1 (en) 2001-03-23 2002-11-14 Fuji Photo Film Co., Ltd. Particle size variable reactor
JP3691428B2 (ja) * 2001-11-12 2005-09-07 三菱電機株式会社 飛行体展示装置
WO2003048388A2 (en) * 2001-12-07 2003-06-12 The University Of Liverpool Immuno polymerase chain reaction assay
US20030175828A1 (en) * 2002-03-15 2003-09-18 Lazar James G. Signal amplification by Hybrid Capture
US20030179381A1 (en) * 2002-03-18 2003-09-25 Fuji Photo Film Co., Ltd. Sensor, color sensor and apparatus for inspection using the same
AU2003251986A1 (en) * 2002-07-17 2004-02-02 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
JP4294946B2 (ja) * 2002-12-13 2009-07-15 富士フイルム株式会社 標的検出装置及び標的検出方法並びに標的検出用基材
US7076127B2 (en) * 2003-01-14 2006-07-11 Fuji Photo Film Co., Ltd. Optical switch and safety apparatus using the same
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
TWI375796B (en) * 2003-04-18 2012-11-01 Becton Dickinson Co Immuno-amplification
EP1649055B1 (en) * 2003-06-27 2009-05-20 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20050214805A1 (en) * 2003-11-10 2005-09-29 Q-Rna, Inc. Methods of detection employing immuno-Q-Amp technology
US20060199194A1 (en) * 2003-11-10 2006-09-07 Q-Rna, Inc. Methods of detection using immuno-Q-Amp technology
JP2007518107A (ja) * 2004-01-13 2007-07-05 ユー．エス． ジェノミクス， インコーポレイテッド ポリマーを使用する溶液中の分析物の検出および定量化
JP2007529758A (ja) * 2004-03-19 2007-10-25 ユー．エス． ジェノミクス， インコーポレイテッド 単一分子の検出のための組成物および方法
EP1747295B1 (en) * 2004-05-12 2012-01-11 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20060204999A1 (en) * 2005-03-14 2006-09-14 Stephen Macevicz Detecting molecular complexes
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US20100035247A1 (en) * 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
US20100294665A1 (en) * 2007-07-12 2010-11-25 Richard Allen Method and system for transferring and/or concentrating a sample
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Also Published As

Publication number Publication date
US6083689A (en) 2000-07-04
EP0819249A4 (en) 1999-04-14
KR19980703574A (ko) 1998-11-05
AU5850396A (en) 1996-10-30
WO1996032640A1 (en) 1996-10-17
EP0819249B1 (en) 2001-10-04
CA2216466A1 (en) 1996-10-17
ATE206520T1 (de) 2001-10-15
JPH11503828A (ja) 1999-03-30
BR9604965A (pt) 1998-07-14
DE69615673D1 (de) 2001-11-08
EP0819249A1 (en) 1998-01-21
PL322650A1 (en) 1998-02-16

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