MX9304113A - Metodo para purificacion de una solucion enzimatica acuosa. - Google Patents

Metodo para purificacion de una solucion enzimatica acuosa.

Info

Publication number
MX9304113A
MX9304113A MX9304113A MX9304113A MX9304113A MX 9304113 A MX9304113 A MX 9304113A MX 9304113 A MX9304113 A MX 9304113A MX 9304113 A MX9304113 A MX 9304113A MX 9304113 A MX9304113 A MX 9304113A
Authority
MX
Mexico
Prior art keywords
purification
enzymatic solution
aqueous enzymatic
aqueous
aluminate
Prior art date
Application number
MX9304113A
Other languages
English (en)
Inventor
Niels-Viktor Nielsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of MX9304113A publication Critical patent/MX9304113A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporciona un método que comprende la adición sumultánea de ácido y aluminato en cantidades controladas, opcionalmente en presencia de un solvente miscible en agua, seguido por el presente método se separan más efectivamente las impurezas de la enzima, la cual permanece en el sobrenadante.
MX9304113A 1992-07-10 1993-07-08 Metodo para purificacion de una solucion enzimatica acuosa. MX9304113A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK92910A DK91092D0 (es) 1992-07-10 1992-07-10

Publications (1)

Publication Number Publication Date
MX9304113A true MX9304113A (es) 1994-04-29

Family

ID=8098903

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9304113A MX9304113A (es) 1992-07-10 1993-07-08 Metodo para purificacion de una solucion enzimatica acuosa.

Country Status (11)

Country Link
US (1) US5733764A (es)
EP (1) EP0651791B1 (es)
JP (1) JPH07508175A (es)
KR (1) KR100278329B1 (es)
AT (1) ATE207116T1 (es)
BR (1) BR9306693A (es)
DE (1) DE69330956T2 (es)
DK (2) DK91092D0 (es)
FI (1) FI116225B (es)
MX (1) MX9304113A (es)
WO (1) WO1994001537A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0828753T3 (da) * 1995-06-02 2003-06-30 Novozymes As A1/Fe-behandling af en proteinopløsning efterfulgt af membranopkoncentrering
US6235205B1 (en) 1996-10-03 2001-05-22 Cytec Technology Corp. Aqueous dispersions
JP4416188B2 (ja) * 1997-07-02 2010-02-17 ワシントン ステイト ユニバーシティ リサーチ ファウンデーション ポテト塊茎からのプロテイナーゼインヒビタータンパク質の単離方法
US6540693B2 (en) 1998-03-03 2003-04-01 Senorx, Inc. Methods and apparatus for securing medical instruments to desired locations in a patients body
US6344026B1 (en) 1998-04-08 2002-02-05 Senorx, Inc. Tissue specimen encapsulation device and method thereof
US6312429B1 (en) 1998-09-01 2001-11-06 Senorx, Inc. Electrosurgical lesion location device
FR2825373B1 (fr) * 2001-05-31 2004-04-30 Tmi Europ Procede de production enzymatique d'un agent de traitement a l'etat fluide
US9408592B2 (en) 2003-12-23 2016-08-09 Senorx, Inc. Biopsy device with aperture orientation and improved tip
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3795586A (en) * 1969-08-11 1974-03-05 Pabst Brewing Co Recovery of enzymes
US3664926A (en) * 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
US4237231A (en) * 1979-11-13 1980-12-02 Uop Inc. Method of purifying glucose isomerase and a composition for storing same
DE3639322A1 (de) * 1986-11-17 1988-05-26 Henkel Kgaa Verfahren zur reinigung und desinfektion von endoskopen und mittel zur durchfuehrung des verfahrens
US4711739A (en) * 1986-12-18 1987-12-08 S. C. Johnson & Son, Inc. Enzyme prespotter composition stabilized with water insoluble polyester or polyether polyol
DE3915277A1 (de) * 1989-05-10 1990-11-15 Henkel Kgaa Verfahren zur reinigung von enzymfluessigkonzentraten
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases

Also Published As

Publication number Publication date
FI950094A (fi) 1995-01-09
KR100278329B1 (ko) 2001-01-15
EP0651791B1 (en) 2001-10-17
JPH07508175A (ja) 1995-09-14
WO1994001537A1 (en) 1994-01-20
DK0651791T3 (da) 2002-01-28
FI116225B (fi) 2005-10-14
BR9306693A (pt) 1998-12-08
DK91092D0 (es) 1992-07-10
KR950702622A (ko) 1995-07-29
DE69330956D1 (de) 2001-11-22
DE69330956T2 (de) 2002-06-20
FI950094A0 (fi) 1995-01-09
ATE207116T1 (de) 2001-11-15
EP0651791A1 (en) 1995-05-10
US5733764A (en) 1998-03-31

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Legal Events

Date Code Title Description
FG Grant or registration
PD Change of proprietorship

Owner name: NOVOZYMES A/S

MM Annulment or lapse due to non-payment of fees