MX384586B - Ensayo de internalización del receptor del gusto. - Google Patents

Ensayo de internalización del receptor del gusto.

Info

Publication number
MX384586B
MX384586B MX2015015448A MX2015015448A MX384586B MX 384586 B MX384586 B MX 384586B MX 2015015448 A MX2015015448 A MX 2015015448A MX 2015015448 A MX2015015448 A MX 2015015448A MX 384586 B MX384586 B MX 384586B
Authority
MX
Mexico
Prior art keywords
taste receptor
receptor internalization
internalization assay
taste
assay
Prior art date
Application number
MX2015015448A
Other languages
English (en)
Other versions
MX2015015448A (es
Inventor
Stephen Gravina
Yuliya Kurash
Original Assignee
Pepsico Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pepsico Inc filed Critical Pepsico Inc
Publication of MX2015015448A publication Critical patent/MX2015015448A/es
Publication of MX384586B publication Critical patent/MX384586B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invención se refiere a un método para identificar una modulador del gusto, caracterizado porque comprende: a) poner en contacto una célula que comprende un receptor del gusto funcional con un compuesto de prueba, en donde la célula es una célula NCI-H716; b) poner en contacto la célula NCI-H716 con un primer reactivo que comprende una etiqueta detectable específica para un núcleo de célula y con un segundo reactivo que comprende una segunda etiqueta detectable específica para el receptor del gusto; y c) detectar la internalización del receptor del gusto, en donde la internalización del receptor del gusto en la presencia del compuesto de prueba indica que el compuesto de prueba es un modulador del gusto potencial y en donde el receptor del gusto funcional es un receptor del gusto endógeno funcional.
MX2015015448A 2013-05-10 2014-05-09 Ensayo de internalización del receptor del gusto. MX384586B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361821978P 2013-05-10 2013-05-10
PCT/US2014/037507 WO2014183041A1 (en) 2013-05-10 2014-05-09 Taste receptor internalization assay

Publications (2)

Publication Number Publication Date
MX2015015448A MX2015015448A (es) 2016-03-15
MX384586B true MX384586B (es) 2025-03-14

Family

ID=51867771

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015015448A MX384586B (es) 2013-05-10 2014-05-09 Ensayo de internalización del receptor del gusto.

Country Status (13)

Country Link
US (1) US9766236B2 (es)
EP (1) EP2994482B1 (es)
JP (1) JP6211685B2 (es)
CN (1) CN105377885B (es)
AU (1) AU2014262540B2 (es)
BR (1) BR112015028082A2 (es)
CA (1) CA2911189A1 (es)
ES (1) ES2776159T3 (es)
HK (1) HK1218302A1 (es)
MX (1) MX384586B (es)
PL (1) PL2994482T3 (es)
RU (1) RU2665812C2 (es)
WO (1) WO2014183041A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2994477A4 (en) 2013-05-10 2017-03-22 Pepsico, Inc. Cells as a model to identify potential taste modulators
US11102995B2 (en) 2016-09-16 2021-08-31 Pepsico, Inc. Compositions and methods for improving taste of non-nutritive sweeteners
EP4298209A1 (en) * 2021-05-07 2024-01-03 Firmenich Incorporated Cells expressing bitter taste receptors and uses thereof
WO2025262048A1 (en) * 2024-06-18 2025-12-26 Firmenich Sa Methods of determining susceptibility of olfactory receptor ligands to adaptation

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4115038A1 (de) * 1991-05-08 1992-11-12 Genentech Inc Neue konjugate, bestehend aus einem glykoprotein und einer nukleinsaeure-bindenden substanz
US6759206B1 (en) * 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
DK1095277T3 (da) * 1998-07-13 2003-04-22 Cellomics Inc System til cellebaseret screening
US7402400B2 (en) 2001-07-03 2008-07-22 Regents Of The University Of California Mammalian sweet taste receptors
JP4339542B2 (ja) * 1999-09-10 2009-10-07 ザ、リージェンツ、オブ、ザ、ユニバーシティ、オブ、カリフォルニア Ｔ２ｒ、すなわち味覚受容体の新規ファミリー
US7368285B2 (en) 2001-03-07 2008-05-06 Senomyx, Inc. Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same
US20080244761A1 (en) * 2001-03-07 2008-10-02 Senomyx, Inc. T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US6955887B2 (en) 2001-03-30 2005-10-18 Senomyx, Inc. Use of T1R hetero-oligomeric taste receptor to screen for compounds that modulate taste signaling
US7763431B1 (en) 2001-03-07 2010-07-27 Senomyx, Inc. Binding assays that use the T1R2/T1R3 (sweet) taste receptor to identify compounds that elicit or modulate sweet taste
US7022488B2 (en) 2003-02-03 2006-04-04 Senomyx, Inc. Functional coupling of T1Rs and T2Rs by Gi proteins, and cells-based assays for the identification of T1R and T2R modulators
WO2003025137A2 (en) 2001-09-18 2003-03-27 Irm, Llc Sweet taste receptors
AU2003261280B2 (en) 2002-07-29 2009-02-05 Senomyx, Inc. Identification of a novel bitter taste receptor, T2R76
WO2004055048A2 (en) 2002-12-18 2004-07-01 Givaudan Sa Chimeric alpha q-gustducin g-proteins
US20050244810A1 (en) * 2003-09-29 2005-11-03 Egan Josephine M Taste signaling in gastrointestinal cells
US20050106571A1 (en) 2003-10-02 2005-05-19 The Regents Of The University Of California Mammalian T1R3 sweet taste receptors
WO2006040337A1 (en) * 2004-10-13 2006-04-20 Evotec Technologies Gmbh Method for identifying compounds that affect a transport of a protein through a membrane trafficking pathway
WO2006044594A2 (en) * 2004-10-15 2006-04-27 Monell Chemical Senses Center Methods for culturing mammalian taste cells
US7799538B2 (en) 2005-03-09 2010-09-21 Trustees Of Dartmouth College Method for identifying agents which modulate GTPase activity involved in insulin-stimulated GLUT4 translocation
CN101605810B (zh) 2005-10-20 2013-07-17 塞诺米克斯公司 嵌合的人甜味-鲜味和鲜味-甜味受体
US20070218456A1 (en) 2006-02-08 2007-09-20 Invitrogen Corporation Cellular assays for signaling receptors
EP2047254B1 (en) 2006-04-20 2015-10-28 Givaudan SA Method relating to sweetness enhancement
EP2035827B1 (en) 2006-06-19 2012-10-17 Givaudan S.A. Nucleic acid, polypeptide and its use
EP2054523B1 (en) * 2006-07-26 2015-11-18 The Coca-Cola Company Methods for identifying modulators of rgs21 activity, compositions comprising an rgs21 modulator, and methods of use thereof to modulate taste sensation
JP5465656B2 (ja) * 2007-03-30 2014-04-09 ジボダン エス エー 調節物質を同定するための方法
US20090117563A1 (en) 2007-06-08 2009-05-07 Bryan Moyer Identification of TRPML3 (MCOLN3) as a Salty Taste Receptor and Use in Assays for Identifying Taste (Salty) Modulators and/or Therapeutics that Modulate Sodium Transport, Absorption or Excretion and/or Aldosterone and/or Vasopressin Production or Release
RU2522456C2 (ru) 2007-08-21 2014-07-10 Синомикс, Инк. Соединения, ингибирующие (блокирующие) горький вкус, способы их применения и получения
CN102369275A (zh) 2009-02-02 2012-03-07 卓莫赛尔公司 新型细胞系和方法
US8795977B2 (en) * 2009-12-02 2014-08-05 Conopco, Inc. Method for screening a potential modulator compound of a taste receptor
CN103338783B (zh) * 2011-01-25 2016-03-09 莫奈尔化学感官中心 提供或调节甜味的组合物和方法及其筛选方法
US9128079B2 (en) * 2011-08-08 2015-09-08 The Coca-Cola Company Methods of using lung or bronchial epithelial cells to identify bitter taste modulators
EP2994477A4 (en) 2013-05-10 2017-03-22 Pepsico, Inc. Cells as a model to identify potential taste modulators

Also Published As

Publication number Publication date
AU2014262540A1 (en) 2015-11-12
WO2014183041A1 (en) 2014-11-13
ES2776159T3 (es) 2020-07-29
EP2994482A4 (en) 2016-11-30
AU2014262540B2 (en) 2016-10-27
US9766236B2 (en) 2017-09-19
EP2994482A1 (en) 2016-03-16
CA2911189A1 (en) 2014-11-13
US20160084834A1 (en) 2016-03-24
HK1218302A1 (zh) 2017-02-10
MX2015015448A (es) 2016-03-15
PL2994482T3 (pl) 2020-06-01
RU2015152792A (ru) 2017-06-20
BR112015028082A2 (pt) 2017-07-25
EP2994482B1 (en) 2020-01-01
CN105377885A (zh) 2016-03-02
JP2016519313A (ja) 2016-06-30
RU2665812C2 (ru) 2018-09-04
JP6211685B2 (ja) 2017-10-11
CN105377885B (zh) 2019-11-05

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