MX376535B - Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa. - Google Patents
Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa.Info
- Publication number
- MX376535B MX376535B MX2017007204A MX2017007204A MX376535B MX 376535 B MX376535 B MX 376535B MX 2017007204 A MX2017007204 A MX 2017007204A MX 2017007204 A MX2017007204 A MX 2017007204A MX 376535 B MX376535 B MX 376535B
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- polypeptide
- sortase
- amino acid
- sub
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 10
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 10
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 10
- 230000021615 conjugation Effects 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000005580 one pot reaction Methods 0.000 title 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 4
- 125000000539 amino acid group Chemical group 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000004471 Glycine Substances 0.000 abstract 1
- 101900206500 Staphylococcus aureus Sortase A Proteins 0.000 abstract 1
- 241000193996 Streptococcus pyogenes Species 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 108090000250 sortase A Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14198534 | 2014-12-17 | ||
| PCT/EP2015/079692 WO2016096787A1 (en) | 2014-12-17 | 2015-12-15 | Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2017007204A MX2017007204A (es) | 2017-08-28 |
| MX376535B true MX376535B (es) | 2025-03-07 |
Family
ID=52338849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017007204A MX376535B (es) | 2014-12-17 | 2015-12-15 | Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US11162127B2 (from) |
| EP (1) | EP3233906B1 (from) |
| JP (1) | JP6605606B2 (from) |
| KR (1) | KR20170098821A (from) |
| CN (1) | CN107001446A (from) |
| BR (1) | BR112017010888A2 (from) |
| CA (1) | CA2966289A1 (from) |
| MX (1) | MX376535B (from) |
| RU (1) | RU2711364C2 (from) |
| SG (1) | SG11201704627XA (from) |
| WO (1) | WO2016096787A1 (from) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA201891066A1 (ru) * | 2015-10-30 | 2018-10-31 | ЭнБиИ-ТЕРАПЬЮТИКС АГ | Антитела к ror1 |
| CN110295157A (zh) * | 2016-08-25 | 2019-10-01 | 北京大学 | 一种金黄色葡萄球菌分选酶a高效突变体 |
| LT3810633T (lt) * | 2018-06-25 | 2024-01-10 | Spero Therapeutics, Inc. | Junginiai |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT410798B (de) | 2001-01-26 | 2003-07-25 | Cistem Biotechnologies Gmbh | Verfahren zur identifizierung, isolierung und herstellung von antigenen gegen ein spezifisches pathogen |
| BRPI0711249A2 (pt) | 2006-05-30 | 2012-03-13 | Genentech, Inc. | Anticorpos, polinucleotídeos, vetores, células hospedeira, métodos para fabricar um anticorpo, para detectar a presença de cd2 em uma amostra biológica, para tratar uma disfunção proliferativa de células b, para inibir proliferaçõa de células b, para tratar câncer, para fabricar um composto conjugado anti-corpo-droga. imunoconjugados,composições farmacêuticas, formulações farmacêuticas, conjugado anticorpo-droga, compostos conjugados anti-corpo-droga, teste para detectar células b e artigo de fabricação |
| SI2032606T1 (sl) * | 2006-05-30 | 2014-02-28 | Genentech, Inc. | Protitelesa in imunokonjugati in njihove uporabe |
| US8247198B2 (en) | 2007-09-21 | 2012-08-21 | Friedrich Srienc | Enzymatic processing in deep eutectic solvents |
| WO2010087994A2 (en) * | 2009-01-30 | 2010-08-05 | Whitehead Institute For Biomedical Research | Methods for ligation and uses thereof |
| US8648176B2 (en) | 2009-02-27 | 2014-02-11 | Massachusetts Institute Of Technology | Engineered proteins with high affinity for DOTA chelates |
| WO2012145522A2 (en) | 2011-04-19 | 2012-10-26 | Georgia Tech Research Corporation | Deep eutectic solvent systems and methods |
| JP2014525904A (ja) * | 2011-06-28 | 2014-10-02 | ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ | タンパク質連結用クリックケミストリーハンドルを設置するためのソルターゼの使用 |
| US9588110B2 (en) | 2011-07-28 | 2017-03-07 | Cell Signaling Technology, Inc. | Multi component antibody based detection technology |
| WO2013153203A1 (en) | 2012-04-12 | 2013-10-17 | Technische Universiteit Eindhoven | Pretreatment of lignocellulosic biomass and recovery of substituents using natural deep eutectic solvents/compound mixtures with low transition temperatures |
| CA2874436A1 (en) | 2012-05-21 | 2013-11-28 | Massachusetts Institute Of Technology | Translocation of non-natural chemical entities through anthrax protective antigen pore |
| US20140030697A1 (en) | 2012-06-14 | 2014-01-30 | Massachusetts Institute Of Technology | Sortase-mediated modification of viral surface proteins |
| US9267127B2 (en) | 2012-06-21 | 2016-02-23 | President And Fellows Of Harvard College | Evolution of bond-forming enzymes |
| EP2867254B1 (en) | 2012-06-27 | 2017-10-25 | F. Hoffmann-La Roche AG | Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof |
| JP6203838B2 (ja) * | 2012-06-27 | 2017-09-27 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 少なくとも２つの異なる結合実体を含む、テーラーメイドの高度に選択的かつ多重特異的なターゲティング実体を選択および作製するための方法、ならびにその使用 |
| WO2014055936A1 (en) | 2012-10-04 | 2014-04-10 | Integenx Inc. | Preservation of biological materials in non-aqueous fluid media |
| GB201303666D0 (en) | 2013-03-01 | 2013-04-17 | Goldsborough Andrew S | Sample fixation and stabilisation |
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| US9631218B2 (en) * | 2013-03-15 | 2017-04-25 | The Trustees Of The University Of Pennsylvania | Sortase-mediated protein purification and ligation |
| US20180104349A9 (en) | 2013-04-28 | 2018-04-19 | Gang Qin | Novel linker, preparation method, and application thereof |
| EP2994491A4 (en) | 2013-05-10 | 2016-12-07 | Whitehead Inst Biomedical Res | IN VITRO PREPARATION OF ERYTHROCYTES WITH SORTASE-PROOF PROTEINS |
| US10260038B2 (en) | 2013-05-10 | 2019-04-16 | Whitehead Institute For Biomedical Research | Protein modification of living cells using sortase |
-
2015
- 2015-12-15 MX MX2017007204A patent/MX376535B/es active IP Right Grant
- 2015-12-15 KR KR1020177015798A patent/KR20170098821A/ko not_active Ceased
- 2015-12-15 RU RU2017122041A patent/RU2711364C2/ru active
- 2015-12-15 WO PCT/EP2015/079692 patent/WO2016096787A1/en not_active Ceased
- 2015-12-15 SG SG11201704627XA patent/SG11201704627XA/en unknown
- 2015-12-15 CA CA2966289A patent/CA2966289A1/en active Pending
- 2015-12-15 JP JP2017531746A patent/JP6605606B2/ja not_active Expired - Fee Related
- 2015-12-15 CN CN201580068342.XA patent/CN107001446A/zh active Pending
- 2015-12-15 EP EP15817154.6A patent/EP3233906B1/en active Active
- 2015-12-15 BR BR112017010888A patent/BR112017010888A2/pt active Search and Examination
-
2017
- 2017-06-16 US US15/625,918 patent/US11162127B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| KR20170098821A (ko) | 2017-08-30 |
| MX2017007204A (es) | 2017-08-28 |
| US11162127B2 (en) | 2021-11-02 |
| CA2966289A1 (en) | 2016-06-23 |
| JP6605606B2 (ja) | 2019-11-13 |
| EP3233906B1 (en) | 2019-05-01 |
| US20170314055A1 (en) | 2017-11-02 |
| BR112017010888A2 (pt) | 2018-02-27 |
| RU2017122041A (ru) | 2019-01-21 |
| RU2711364C2 (ru) | 2020-01-16 |
| RU2017122041A3 (from) | 2019-07-17 |
| WO2016096787A1 (en) | 2016-06-23 |
| EP3233906A1 (en) | 2017-10-25 |
| SG11201704627XA (en) | 2017-07-28 |
| JP2018505661A (ja) | 2018-03-01 |
| CN107001446A (zh) | 2017-08-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |