MX375554B - Dispositivo de doble poro. - Google Patents
Dispositivo de doble poro.Info
- Publication number
- MX375554B MX375554B MX2016008367A MX2016008367A MX375554B MX 375554 B MX375554 B MX 375554B MX 2016008367 A MX2016008367 A MX 2016008367A MX 2016008367 A MX2016008367 A MX 2016008367A MX 375554 B MX375554 B MX 375554B
- Authority
- MX
- Mexico
- Prior art keywords
- chamber
- pore
- pore device
- double pore
- communication
- Prior art date
Links
- 239000011148 porous material Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44765—Apparatus specially adapted therefor of the counter-flow type
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Nanotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Dispersion Chemistry (AREA)
- Power Engineering (AREA)
- Computer Hardware Design (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Composite Materials (AREA)
Abstract
Se proporciona un dispositivo que comprende una cámara superior, una cámara intermedia y una cámara inferior, donde la cámara superior está en comunicación con la cámara intermedia a través de un primer poro, y la cámara intermedia está en comunicación con la cámara inferior a través de un segundo poro, donde el primer poro y el segundo poro son de aproximadamente 1 nm a aproximadamente 100 nm de diámetro, y están de aproximadamente 10 nm a aproximadamente 1000 nm de separación entre sí, y donde cada una de las cámaras comprende un electrodo para conectarse con una fuente de alimentación. También se proporcionan los métodos para usar el dispositivo, particularmente para secuenciar un polinucleótido.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161572843P | 2011-07-20 | 2011-07-20 | |
| PCT/US2012/047107 WO2013012881A2 (en) | 2011-07-20 | 2012-07-18 | Dual-pore device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX375554B true MX375554B (es) | 2025-03-06 |
Family
ID=47558702
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016008367A MX375554B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
| MX2013008539A MX342569B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013008539A MX342569B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US8961763B2 (es) |
| EP (3) | EP2734840B3 (es) |
| JP (1) | JP6364348B2 (es) |
| KR (2) | KR101820834B1 (es) |
| CN (2) | CN103328973B (es) |
| AU (1) | AU2012284137C1 (es) |
| CA (2) | CA2944478A1 (es) |
| ES (2) | ES2565634T7 (es) |
| IL (1) | IL228337A (es) |
| MX (2) | MX375554B (es) |
| WO (1) | WO2013012881A2 (es) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| CN103328973B (zh) | 2011-07-20 | 2015-04-01 | 加利福尼亚大学董事会 | 双孔装置 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| JP6063693B2 (ja) * | 2012-10-03 | 2017-01-18 | 株式会社日立ハイテクノロジーズ | 分析装置及び分析方法 |
| US20140099726A1 (en) * | 2012-10-10 | 2014-04-10 | Two Pore Guys, Inc. | Device for characterizing polymers |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| US10261065B2 (en) * | 2013-02-28 | 2019-04-16 | The University Of North Carolina At Chapel Hill | Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis |
| ES2704902T3 (es) * | 2013-05-06 | 2019-03-20 | Two Pore Guys Inc | Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión |
| KR102245192B1 (ko) * | 2013-05-06 | 2021-04-29 | 온테라 인크. | 나노포어를 이용한 표적 검출 |
| EP3014264A4 (en) * | 2013-06-25 | 2016-11-16 | Two Pore Guys Inc | QUANTIFICATION OF MULTIPLEXED BIOMARKERS BY NANOPORENT ANALYSIS OF BIOMARKER POLYMER COMPLEXES |
| WO2015031399A1 (en) | 2013-08-26 | 2015-03-05 | Two Pore Guys, Inc. | Molecule detection using boronic acid substituted probes |
| CN105531360B (zh) * | 2013-11-08 | 2017-07-11 | 株式会社日立高新技术 | Dna输送控制设备及其制造方法、以及dna测序装置 |
| US10190161B2 (en) * | 2014-04-03 | 2019-01-29 | Stmicroelectronics S.R.L. | Apparatus and method for nucleic acid sequencing based on nanowire detectors |
| US9765326B2 (en) * | 2014-04-03 | 2017-09-19 | Stmicroelectronics S.R.L. | Apparatus and method for nucleic acid sequencing based on nanochannels |
| JP6513705B2 (ja) | 2014-05-13 | 2019-05-15 | ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ | 固体状態ナノ細孔を用いた修飾生物学的分子の選択的分析 |
| US20170074855A1 (en) * | 2014-05-15 | 2017-03-16 | Two Pore Guys, Inc. | Scaffold Data Storage and Target Detection in a Sample Using a Nanopore |
| IL251274B2 (en) * | 2014-09-26 | 2026-01-01 | Ontera Inc | Target Sequence Detection Using Synthetic Detector Nanochannel Sensors |
| JP6472208B2 (ja) * | 2014-10-24 | 2019-02-20 | 株式会社日立ハイテクノロジーズ | 核酸搬送制御デバイス及びその製造方法、並びに核酸シーケンシング装置 |
| KR20170106487A (ko) * | 2015-02-02 | 2017-09-20 | 투 포어 가이즈, 인코포레이티드 | 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 |
| KR101945366B1 (ko) | 2015-03-11 | 2019-02-07 | 투 포어 가이즈, 인코포레이티드 | 경쟁 검정법을 통한 저분자의 나노포어 검출 |
| US9983191B2 (en) | 2015-03-11 | 2018-05-29 | Two Pore Guys, Inc. | Nanopore detection of small molecules through competition assays |
| IN2015CH01270A (es) * | 2015-03-13 | 2015-04-10 | Wipro Ltd | |
| US10859562B2 (en) | 2016-02-29 | 2020-12-08 | Iridia, Inc. | Methods, compositions, and devices for information storage |
| US10438662B2 (en) | 2016-02-29 | 2019-10-08 | Iridia, Inc. | Methods, compositions, and devices for information storage |
| US10640822B2 (en) | 2016-02-29 | 2020-05-05 | Iridia, Inc. | Systems and methods for writing, reading, and controlling data stored in a polymer |
| US10488394B2 (en) * | 2016-03-21 | 2019-11-26 | Ontera Inc. | Wafer-scale assembly of insulator-membrane-insulator devices for nanopore sensing |
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| JP2017187443A (ja) * | 2016-04-08 | 2017-10-12 | Towa株式会社 | 分析装置用ポリマー膜、分析装置、分析装置用基板、分析装置用ポリマー膜の製造方法、および分析装置用基板の製造方法 |
| US11333655B2 (en) | 2016-09-07 | 2022-05-17 | The Board Of Trustees Of The University Of Illinois | Nanopore-based system for trapping, controlled displacement, and sequencing of (bio)macromolecules |
| MX2018013686A (es) | 2016-10-24 | 2019-05-02 | Two Pore Guys Inc | Abundancia fraccional de secuencias de polinucleotidos en una muestra. |
| KR101895123B1 (ko) * | 2016-10-24 | 2018-09-04 | 고려대학교 산학협력단 | 이중 나노 포어 소자 및 이의 제조 방법 |
| US10650312B2 (en) | 2016-11-16 | 2020-05-12 | Catalog Technologies, Inc. | Nucleic acid-based data storage |
| ES2979182T3 (es) | 2016-11-16 | 2024-09-24 | Catalog Tech Inc | Almacenamiento de datos basado en ácidos nucleicos |
| WO2018183380A1 (en) * | 2017-03-28 | 2018-10-04 | Two Pore Guys, Inc. | Target polynucleotide detection and sequencing by incorporation of modified nucleotides for nanopore analysis |
| KR101903723B1 (ko) | 2017-04-27 | 2018-10-02 | 주식회사라이브셀인스트루먼트 | 나노포어 멤브레인 격자 구조물 및 자성체 샘플 회전 구조물이 구비된 생체조직 투명화 장치 |
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| JP7038147B2 (ja) * | 2017-06-21 | 2022-03-17 | ヌーマ バイオ インコーポレイテッド | 二重細孔制御およびセンサデバイス |
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| CN107402199B (zh) * | 2017-07-31 | 2019-09-10 | 京东方科技集团股份有限公司 | 基因测序芯片及其测序方法以及基因测序装置 |
| JP6831521B2 (ja) * | 2018-02-15 | 2021-02-17 | アイポア株式会社 | 分析素子及び分析装置 |
| AU2019222673B2 (en) | 2018-02-16 | 2022-01-20 | Illumina, Inc. | Device for sequencing |
| KR20200132921A (ko) | 2018-03-16 | 2020-11-25 | 카탈로그 테크놀로지스, 인크. | 핵산-기반 데이터를 저장하기 위한 화학적 방법들 |
| KR102956991B1 (ko) | 2018-05-16 | 2026-04-24 | 카탈로그 테크놀로지스, 인크. | 핵산-기반 데이터를 저장하기 위한 조성물 및 방법 |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
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| JP7082020B2 (ja) | 2018-09-28 | 2022-06-07 | 株式会社アドバンテスト | ポアチップケースおよび微粒子測定システム |
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| CN109612890B (zh) * | 2018-12-28 | 2021-02-02 | 瑞芯智造(深圳)科技有限公司 | 一种微粒计数系统及应用、检测金属离子和微粒的方法 |
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| CN115125131A (zh) * | 2021-03-29 | 2022-09-30 | 上海近观科技有限责任公司 | 基于场效应晶体管的纳米孔阵列结构及其制备方法 |
| JP2024512838A (ja) | 2021-03-31 | 2024-03-21 | イルミナ インコーポレイテッド | 分子検出のためのスケーラブル回路 |
| US12455277B2 (en) | 2021-09-30 | 2025-10-28 | Illumina, Inc. | Isolation of cells in a nanopore sensor array |
| CN120435657A (zh) | 2022-10-28 | 2025-08-05 | 格罗宁根大学 | 蛋白质的基于纳米孔的分析 |
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|---|---|---|---|---|
| US6267872B1 (en) * | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
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2012
- 2012-07-18 CN CN201280005639.8A patent/CN103328973B/zh active Active
- 2012-07-18 KR KR1020167005366A patent/KR101820834B1/ko active Active
- 2012-07-18 EP EP12815570.2A patent/EP2734840B3/en active Active
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- 2012-07-18 WO PCT/US2012/047107 patent/WO2013012881A2/en not_active Ceased
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