MX375554B - Dispositivo de doble poro. - Google Patents
Dispositivo de doble poro.Info
- Publication number
- MX375554B MX375554B MX2016008367A MX2016008367A MX375554B MX 375554 B MX375554 B MX 375554B MX 2016008367 A MX2016008367 A MX 2016008367A MX 2016008367 A MX2016008367 A MX 2016008367A MX 375554 B MX375554 B MX 375554B
- Authority
- MX
- Mexico
- Prior art keywords
- chamber
- pore
- pore device
- double pore
- communication
- Prior art date
Links
- 239000011148 porous material Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44765—Apparatus specially adapted therefor of the counter-flow type
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Nanotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Dispersion Chemistry (AREA)
- Power Engineering (AREA)
- Computer Hardware Design (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Composite Materials (AREA)
Abstract
Se proporciona un dispositivo que comprende una cámara superior, una cámara intermedia y una cámara inferior, donde la cámara superior está en comunicación con la cámara intermedia a través de un primer poro, y la cámara intermedia está en comunicación con la cámara inferior a través de un segundo poro, donde el primer poro y el segundo poro son de aproximadamente 1 nm a aproximadamente 100 nm de diámetro, y están de aproximadamente 10 nm a aproximadamente 1000 nm de separación entre sí, y donde cada una de las cámaras comprende un electrodo para conectarse con una fuente de alimentación. También se proporcionan los métodos para usar el dispositivo, particularmente para secuenciar un polinucleótido.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161572843P | 2011-07-20 | 2011-07-20 | |
| PCT/US2012/047107 WO2013012881A2 (en) | 2011-07-20 | 2012-07-18 | Dual-pore device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX375554B true MX375554B (es) | 2025-03-06 |
Family
ID=47558702
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013008539A MX342569B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
| MX2016008367A MX375554B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013008539A MX342569B (es) | 2011-07-20 | 2012-07-18 | Dispositivo de doble poro. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US8961763B2 (es) |
| EP (3) | EP3318872A1 (es) |
| JP (1) | JP6364348B2 (es) |
| KR (2) | KR101820834B1 (es) |
| CN (2) | CN104774757B (es) |
| AU (1) | AU2012284137C1 (es) |
| CA (2) | CA2944478A1 (es) |
| ES (2) | ES2659343T3 (es) |
| IL (1) | IL228337A (es) |
| MX (2) | MX342569B (es) |
| WO (1) | WO2013012881A2 (es) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| EP3318872A1 (en) | 2011-07-20 | 2018-05-09 | The Regents of the University of California | Dual-pore device |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| JP6063693B2 (ja) * | 2012-10-03 | 2017-01-18 | 株式会社日立ハイテクノロジーズ | 分析装置及び分析方法 |
| US20140099726A1 (en) * | 2012-10-10 | 2014-04-10 | Two Pore Guys, Inc. | Device for characterizing polymers |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| KR20150132125A (ko) * | 2013-02-28 | 2015-11-25 | 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 | 거대분자의 통제된 포획, 고정, 및 전달을 위한 통합된 부품을 가진 나노유체 장치 및 관련 분석 방법 |
| ES2704902T3 (es) | 2013-05-06 | 2019-03-20 | Two Pore Guys Inc | Un método de detección de objetivos biológicos usando un nanoporo y un agente de unión a proteínas de fusión |
| KR102245192B1 (ko) | 2013-05-06 | 2021-04-29 | 온테라 인크. | 나노포어를 이용한 표적 검출 |
| EP3014264A4 (en) * | 2013-06-25 | 2016-11-16 | Two Pore Guys Inc | QUANTIFICATION OF MULTIPLEXED BIOMARKERS BY NANOPORENT ANALYSIS OF BIOMARKER POLYMER COMPLEXES |
| EP3039155B1 (en) | 2013-08-26 | 2019-10-09 | Ontera Inc. | Molecule detection using boronic acid substituted probes |
| US10253362B2 (en) | 2013-11-08 | 2019-04-09 | Hitachi High-Technologies Corporation | DNA transport control device and method for producing same, as well as DNA sequencing device |
| US9765326B2 (en) * | 2014-04-03 | 2017-09-19 | Stmicroelectronics S.R.L. | Apparatus and method for nucleic acid sequencing based on nanochannels |
| US10190161B2 (en) | 2014-04-03 | 2019-01-29 | Stmicroelectronics S.R.L. | Apparatus and method for nucleic acid sequencing based on nanowire detectors |
| CN106662568A (zh) | 2014-05-13 | 2017-05-10 | 韦克福里斯特大学健康学院 | 利用固态纳米孔选择性分析改性的生物分子 |
| WO2015176034A1 (en) * | 2014-05-15 | 2015-11-19 | Two Pore Guys, Inc. | Scaffold data storage and target detection in a sample using a nanopore |
| KR20170064540A (ko) * | 2014-09-26 | 2017-06-09 | 투 포어 가이즈, 인코포레이티드 | 합성 프로브의 나노포어 감지에 의한 표적 서열 검출 |
| JP6472208B2 (ja) * | 2014-10-24 | 2019-02-20 | 株式会社日立ハイテクノロジーズ | 核酸搬送制御デバイス及びその製造方法、並びに核酸シーケンシング装置 |
| HK1245348A1 (zh) * | 2015-02-02 | 2018-08-24 | 奥特拉公司 | 来自样品背景的靶多核苷酸的纳米孔隙检测 |
| US9983191B2 (en) | 2015-03-11 | 2018-05-29 | Two Pore Guys, Inc. | Nanopore detection of small molecules through competition assays |
| CN107430107B (zh) | 2015-03-11 | 2019-08-30 | 双孔人公司 | 通过竞争分析的小分子的纳米孔隙检测 |
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| US11333655B2 (en) | 2016-09-07 | 2022-05-17 | The Board Of Trustees Of The University Of Illinois | Nanopore-based system for trapping, controlled displacement, and sequencing of (bio)macromolecules |
| KR101895123B1 (ko) * | 2016-10-24 | 2018-09-04 | 고려대학교 산학협력단 | 이중 나노 포어 소자 및 이의 제조 방법 |
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| JP6831521B2 (ja) * | 2018-02-15 | 2021-02-17 | アイポア株式会社 | 分析素子及び分析装置 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6267872B1 (en) * | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
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| ATE544063T1 (de) * | 2008-07-17 | 2012-02-15 | Koninkl Philips Electronics Nv | Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse |
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| JP5427722B2 (ja) * | 2010-07-28 | 2014-02-26 | 株式会社日立ハイテクノロジーズ | ナノポア式分析装置及び試料分析用チャンバ |
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-
2012
- 2012-07-18 EP EP17208398.2A patent/EP3318872A1/en not_active Withdrawn
- 2012-07-18 WO PCT/US2012/047107 patent/WO2013012881A2/en not_active Ceased
- 2012-07-18 CN CN201510199107.8A patent/CN104774757B/zh active Active
- 2012-07-18 US US13/882,191 patent/US8961763B2/en active Active
- 2012-07-18 JP JP2014521721A patent/JP6364348B2/ja active Active
- 2012-07-18 CA CA2944478A patent/CA2944478A1/en not_active Abandoned
- 2012-07-18 ES ES15190021.4T patent/ES2659343T3/es active Active
- 2012-07-18 ES ES12815570.2T patent/ES2565634T7/es active Active
- 2012-07-18 KR KR1020167005366A patent/KR101820834B1/ko active Active
- 2012-07-18 CN CN201280005639.8A patent/CN103328973B/zh active Active
- 2012-07-18 MX MX2013008539A patent/MX342569B/es active IP Right Grant
- 2012-07-18 EP EP12815570.2A patent/EP2734840B3/en active Active
- 2012-07-18 MX MX2016008367A patent/MX375554B/es unknown
- 2012-07-18 CA CA2823787A patent/CA2823787C/en active Active
- 2012-07-18 KR KR1020137023965A patent/KR101661297B1/ko active Active
- 2012-07-18 EP EP15190021.4A patent/EP2988128B1/en active Active
- 2012-07-18 AU AU2012284137A patent/AU2012284137C1/en active Active
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2013
- 2013-09-10 IL IL228337A patent/IL228337A/en active IP Right Grant
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2014
- 2014-11-29 US US14/556,134 patent/US9863912B2/en active Active
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