MX374934B - Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas. - Google Patents

Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas.

Info

Publication number
MX374934B
MX374934B MX2017005295A MX2017005295A MX374934B MX 374934 B MX374934 B MX 374934B MX 2017005295 A MX2017005295 A MX 2017005295A MX 2017005295 A MX2017005295 A MX 2017005295A MX 374934 B MX374934 B MX 374934B
Authority
MX
Mexico
Prior art keywords
selection marker
protein production
cell transfection
novel selection
cell lines
Prior art date
Application number
MX2017005295A
Other languages
English (en)
Other versions
MX2017005295A (es
Inventor
Bruno Dumas
Nabil Lounis
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of MX2017005295A publication Critical patent/MX2017005295A/es
Publication of MX374934B publication Critical patent/MX374934B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere al campo de la producción industrial de proteínas. La invención proporciona un nuevo sistema de expresión que usa dihidroorotato-deshidrogenasa (DHODH) como un marcador de selección en combinación con leflunomida o uno de sus metabolitos, principalmente para uso en líneas celulares de mamíferos. Asimismo se proporcionan vectores de expresión que codifican la DHODH, líneas celulares que comprenden dichos vectores y métodos para producir proteínas recombinantes.
MX2017005295A 2014-10-23 2015-10-22 Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas. MX374934B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14306689 2014-10-23
PCT/EP2015/074550 WO2016062837A1 (en) 2014-10-23 2015-10-22 Novel selection marker for cell transfection and protein production

Publications (2)

Publication Number Publication Date
MX2017005295A MX2017005295A (es) 2017-07-28
MX374934B true MX374934B (es) 2025-03-06

Family

ID=51799064

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005295A MX374934B (es) 2014-10-23 2015-10-22 Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas.

Country Status (11)

Country Link
US (3) US10557125B2 (es)
EP (1) EP3209770B1 (es)
CN (1) CN107109435B (es)
AU (1) AU2015334872B2 (es)
CA (1) CA2965202C (es)
DK (1) DK3209770T3 (es)
ES (1) ES2769877T3 (es)
HU (1) HUE048525T2 (es)
MX (1) MX374934B (es)
SG (1) SG11201702516UA (es)
WO (1) WO2016062837A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CL2016001661A1 (es) 2016-06-29 2017-03-03 Univ Chile Promotor hibrido de ß-actina (de cricetulus griseus) y citomegalovirus (cmv), con región rica en dinucleótidos, citosina-guanina, vectores, líneas celulares, procedimiento para producir proteínas recombinantes.
EP3752605A4 (en) * 2018-02-14 2021-12-15 The University of Houston System PROBE FOR UNIVERSAL DETECTION OF CIRCULATING TUMOR CELLS
US12529071B2 (en) 2019-03-19 2026-01-20 Sanofi Selection marker-comprising cell line and uses thereof for protein production
JP2023527163A (ja) * 2020-05-19 2023-06-27 ラモット・アット・テル・アビブ・ユニバーシテイ・リミテッド 核酸送達用の脂質粒子およびその臨床応用
EP4541812A3 (en) 2020-09-21 2025-06-11 Sanofi Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (ko) 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법
US20030166201A1 (en) 2001-04-30 2003-09-04 Jensen Michael C. Selection systems for genetically modified cells
GB0808029D0 (en) * 2008-05-01 2008-06-11 F2G Ltd Antifungal target
AU2010247938B2 (en) * 2009-05-11 2016-02-18 Pelican Technology Holdings, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
US20140031383A1 (en) * 2011-02-08 2014-01-30 Dana-Farber Cancer Institute, Inc. Methods for treatment of melanoma
EP2674495A1 (en) 2012-06-14 2013-12-18 Sanofi CHO expression system
EP2935581A4 (en) * 2012-12-21 2016-07-27 Merck Sharp & Dohme EXPRESSION VECTORS FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS

Also Published As

Publication number Publication date
US11384342B2 (en) 2022-07-12
HUE048525T2 (hu) 2020-07-28
US12203103B2 (en) 2025-01-21
CA2965202C (en) 2023-02-28
US10557125B2 (en) 2020-02-11
US20200199543A1 (en) 2020-06-25
ES2769877T3 (es) 2020-06-29
EP3209770A1 (en) 2017-08-30
AU2015334872B2 (en) 2021-12-16
CA2965202A1 (en) 2016-04-28
MX2017005295A (es) 2017-07-28
CN107109435B (zh) 2021-10-22
SG11201702516UA (en) 2017-04-27
AU2015334872A1 (en) 2017-06-08
CN107109435A (zh) 2017-08-29
DK3209770T3 (da) 2020-02-24
US20230025088A1 (en) 2023-01-26
EP3209770B1 (en) 2019-11-27
WO2016062837A1 (en) 2016-04-28
US20170335292A1 (en) 2017-11-23

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