MX371515B - Metodo y dispositivo para la recoleccion y amplificacion de acidos nucleicos circulantes. - Google Patents

Metodo y dispositivo para la recoleccion y amplificacion de acidos nucleicos circulantes.

Info

Publication number
MX371515B
MX371515B MX2016001159A MX2016001159A MX371515B MX 371515 B MX371515 B MX 371515B MX 2016001159 A MX2016001159 A MX 2016001159A MX 2016001159 A MX2016001159 A MX 2016001159A MX 371515 B MX371515 B MX 371515B
Authority
MX
Mexico
Prior art keywords
collection
nucleic acids
amplification
circulating nucleic
fraction
Prior art date
Application number
MX2016001159A
Other languages
English (en)
Other versions
MX2016001159A (es
Inventor
Charles Heller Ryan
Richard Nelson John
Leeming Kvam Erik
Andrew Grossmann Gregory
Jean Finehout Erin
Michael Puleo Christopher
Patrick Waters William
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of MX2016001159A publication Critical patent/MX2016001159A/es
Publication of MX371515B publication Critical patent/MX371515B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a métodos para la recolección y amplificación de los ácidos nucleicos circulantes de una fracción no celular de una muestra biológica. Los ácidos nucleicos circulantes se extraen de la fracción no celular y son circularizados para generar los círculos del ácido nucleico monocatenario, que se amplifican posteriormente por amplificación circular ondulante utilizando cebadores aleatorios para producir una biblioteca amplificada. Asimismo, se proporcionan dispositivos para la recolección de una fracción no celular de una muestra biológica. El dispositivo incluye una membrana de filtración y una matriz sólida seca, que está en contacto directo con la membrana de filtración.
MX2016001159A 2013-07-26 2014-07-24 Metodo y dispositivo para la recoleccion y amplificacion de acidos nucleicos circulantes. MX371515B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/952,173 US9644232B2 (en) 2013-07-26 2013-07-26 Method and device for collection and amplification of circulating nucleic acids
PCT/US2014/047953 WO2015013486A1 (en) 2013-07-26 2014-07-24 Method and device for collection and amplification of circulating nucleic acids

Publications (2)

Publication Number Publication Date
MX2016001159A MX2016001159A (es) 2016-04-29
MX371515B true MX371515B (es) 2020-01-31

Family

ID=51300879

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016001159A MX371515B (es) 2013-07-26 2014-07-24 Metodo y dispositivo para la recoleccion y amplificacion de acidos nucleicos circulantes.

Country Status (12)

Country Link
US (2) US9644232B2 (es)
EP (2) EP3470532B1 (es)
JP (1) JP6647715B2 (es)
KR (1) KR102313174B1 (es)
CN (1) CN105392900A (es)
AU (1) AU2014293096B2 (es)
CA (1) CA2917437C (es)
ES (1) ES2720435T3 (es)
IL (1) IL243673B (es)
MX (1) MX371515B (es)
SG (2) SG11201600615VA (es)
WO (1) WO2015013486A1 (es)

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Also Published As

Publication number Publication date
KR20160034306A (ko) 2016-03-29
KR102313174B1 (ko) 2021-10-15
WO2015013486A1 (en) 2015-01-29
CA2917437C (en) 2023-08-22
JP6647715B2 (ja) 2020-02-14
US11142758B2 (en) 2021-10-12
AU2014293096B2 (en) 2020-04-16
CN105392900A (zh) 2016-03-09
EP3024944B1 (en) 2019-03-13
ES2720435T3 (es) 2019-07-22
IL243673B (en) 2020-03-31
SG10201705894PA (en) 2017-08-30
JP2016530878A (ja) 2016-10-06
EP3470532B1 (en) 2021-12-15
IL243673A0 (en) 2016-03-31
EP3024944A1 (en) 2016-06-01
CA2917437A1 (en) 2015-01-29
AU2014293096A1 (en) 2016-01-28
MX2016001159A (es) 2016-04-29
US20150031035A1 (en) 2015-01-29
US9644232B2 (en) 2017-05-09
US20170211059A1 (en) 2017-07-27
SG11201600615VA (en) 2016-03-30
EP3470532A1 (en) 2019-04-17

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