MX363037B - Produccion y aplicacion de cultivos protozoarios de histomonas meleagridis (h. meleagridis). - Google Patents

Produccion y aplicacion de cultivos protozoarios de histomonas meleagridis (h. meleagridis).

Info

Publication number
MX363037B
MX363037B MX2014016016A MX2014016016A MX363037B MX 363037 B MX363037 B MX 363037B MX 2014016016 A MX2014016016 A MX 2014016016A MX 2014016016 A MX2014016016 A MX 2014016016A MX 363037 B MX363037 B MX 363037B
Authority
MX
Mexico
Prior art keywords
meleagridis
histomonas
culture
bacterial strain
cells
Prior art date
Application number
MX2014016016A
Other languages
English (en)
Other versions
MX2014016016A (es
Inventor
Hess Michael
Ganas Petra
Original Assignee
Veterinaermedizinische Univ Wien
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Veterinaermedizinische Univ Wien filed Critical Veterinaermedizinische Univ Wien
Publication of MX2014016016A publication Critical patent/MX2014016016A/es
Publication of MX363037B publication Critical patent/MX363037B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/68—Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/002—Protozoa antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0258—Escherichia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención da aconocer un método para producir un cultivo de cepa bacteriana única de Histomonas meleagridis (H. meleagridis), siendo caracterizado el método por los siguientes pasos: (a) proporcionar un cultivo xénico de H. meleagridis que comprenda células de H. meleagridis con una flora bacteriana tipo silvestre, (b) tratar el cultivo xénico con una mescla de antibióticos matando así la flora bacteriana tipo silvestre, (c) centrifugado y lavado de las células de H. meleagridis, (d) controlar la eficacia del paso (b), (e) resuspender las células lavadas de H. meleagridis, (f) agregar uno o más cepas(S) bacteriana (s) única (s) a las células resuspendidas de H. meleagridis, y (g) cultivar tal (es) cepa (s) bacteriana (s) única (s) con las células resuspendidas de H. meleagridis a fin de obtener un cultivo de cepa bacteriana única de H. meleagridis. La invención describe además la formulación de una vacuna que consta de un componente Histomonas que consiste en un cultivo atenuado de Histomonas meleagridis, un componente bacteriano que consiste de uno o más cultivos de una cepa bacteriana única, y compuestos de formulación no biológicos farmacéuticamente aceptables.
MX2014016016A 2012-07-02 2013-07-02 Produccion y aplicacion de cultivos protozoarios de histomonas meleagridis (h. meleagridis). MX363037B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12174582.2A EP2682457A1 (en) 2012-07-02 2012-07-02 Production and application of protozoa cultures of Histomonas meleagridis (H. meleagridis)
PCT/EP2013/063889 WO2014006018A1 (en) 2012-07-02 2013-07-02 Production and application of protozoa cultures of histomonas meleagridis (h. meleagridis)

Publications (2)

Publication Number Publication Date
MX2014016016A MX2014016016A (es) 2015-12-07
MX363037B true MX363037B (es) 2019-03-01

Family

ID=48793189

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014016016A MX363037B (es) 2012-07-02 2013-07-02 Produccion y aplicacion de cultivos protozoarios de histomonas meleagridis (h. meleagridis).

Country Status (11)

Country Link
US (2) US9833501B2 (es)
EP (2) EP2682457A1 (es)
JP (2) JP6386452B2 (es)
CN (1) CN104471054A (es)
AU (1) AU2013286002B2 (es)
BR (1) BR112014032937A2 (es)
CA (1) CA2877539A1 (es)
CL (1) CL2014003549A1 (es)
ES (1) ES2893834T3 (es)
MX (1) MX363037B (es)
WO (1) WO2014006018A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108633843B (zh) * 2018-04-18 2020-10-27 中国科学院水生生物研究所 一种鲩肠袋虫的体外培养方法及使用的培养基的制备方法
CN110616149A (zh) * 2019-10-11 2019-12-27 扬州大学 一种鸡源火鸡组织滴虫传代致弱株、其制备方法及用途
CN112322765B (zh) * 2020-10-15 2022-11-22 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） 快速检测鸡球虫与组织滴虫的特异性引物、试剂盒及方法
EP4112717A1 (en) 2021-06-29 2023-01-04 Veterinärmedizinische Universität Wien Attenuated histomonas meleagridis strain and vaccine

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT502702B1 (de) 2005-05-12 2008-05-15 Veterinaermedizinische Uni Wie Verfahren zur herstellung und anwendung von protozoenkulturen

Also Published As

Publication number Publication date
US20150157698A1 (en) 2015-06-11
JP2015522269A (ja) 2015-08-06
EP2867356A1 (en) 2015-05-06
JP6386452B2 (ja) 2018-09-05
CL2014003549A1 (es) 2015-08-07
BR112014032937A2 (pt) 2017-08-01
CA2877539A1 (en) 2014-01-09
US9833501B2 (en) 2017-12-05
ES2893834T3 (es) 2022-02-10
US20180117131A1 (en) 2018-05-03
AU2013286002B2 (en) 2019-01-31
WO2014006018A1 (en) 2014-01-09
JP2018148893A (ja) 2018-09-27
EP2682457A1 (en) 2014-01-08
US10350281B2 (en) 2019-07-16
EP2867356B1 (en) 2021-08-18
CN104471054A (zh) 2015-03-25
AU2013286002A1 (en) 2015-01-22
MX2014016016A (es) 2015-12-07

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