BR112014032937A2 - produção e aplicação de culturas de protozoários de histomonas meleagridis (h. meleagridis) - Google Patents
produção e aplicação de culturas de protozoários de histomonas meleagridis (h. meleagridis)Info
- Publication number
- BR112014032937A2 BR112014032937A2 BR112014032937A BR112014032937A BR112014032937A2 BR 112014032937 A2 BR112014032937 A2 BR 112014032937A2 BR 112014032937 A BR112014032937 A BR 112014032937A BR 112014032937 A BR112014032937 A BR 112014032937A BR 112014032937 A2 BR112014032937 A2 BR 112014032937A2
- Authority
- BR
- Brazil
- Prior art keywords
- meleagridis
- cells
- culture
- histomonas
- bacterial strain
- Prior art date
Links
- 241000948220 Histomonas meleagridis Species 0.000 title abstract 21
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 230000001580 bacterial effect Effects 0.000 abstract 16
- 239000000203 mixture Substances 0.000 abstract 6
- 238000009472 formulation Methods 0.000 abstract 4
- 241000948219 Histomonas Species 0.000 abstract 2
- 239000003242 anti bacterial agent Substances 0.000 abstract 2
- 229940088710 antibiotic agent Drugs 0.000 abstract 2
- 230000002238 attenuated effect Effects 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229960005486 vaccine Drugs 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/68—Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0258—Escherichia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
summary: the invention discloses a method for producing a single bacterial strain culture of histomonas meleagridis (h. meleagridis), the method being characterised by the following steps: (a) providing a xenic culture of h. meleagridis comprising h. meleagridis cells with a wild type bacterial flora, (b) treating the xenic culture with a mixture of antibiotics thereby killing the wild type bacterial flora, (c) centrifuging and washing the h. meleagridis cells, (d) controlling effectiveness of step (b), (e) resuspending the washed h. meleagridis cells, (f) adding one or more single bacterial strain(s) to the resuspended h. meleagridis cells, and (g) culturing the one or more single bacterial strain(s) with the resuspended h. meleagridis cells so as to obtain a single bacterial strain culture of h. meleagridis. the invention further discloses a vaccine formulation consisting of a histomonas component consisting of an attenuated culture of histomonas meleagridis, a bacterial component consisting of one or more cultures of a single bacterial strain, and pharmaceutically acceptable non-biological formulation compounds. ___________________________________________________________________ resumo patente de invenção: "produção e aplicação de culturas de protozoários de histomonas meleagridis (h. meleagridis)". a presente invenção refere-se a um método para produzir uma cultura de cepa bacteriana simples de histomonas meleagridis (h. meleagridis), em que o método é caracterizado pelas seguintes etapas: (a) fornecer uma cultura xênica de h. meleagridis que compreende células de h. meleagridis com uma flora bacteriana do tipo selvagem, (b) tratar a cultura xênica com uma mistura de antibióticos exterminando, assim, a flora bacteriana do tipo selvagem, (c) centrifugar e lavar as células de h. meleagridis, (d) controlar a eficácia da etapa (b), (e) ressuspender as células lavadas de h. meleagridis, (f) adicionar uma ou mais cepa(s) bacteriana(s) simples às células ressuspensas de h. meleagridis, e (g) cultivar a uma ou mais cepa(s) bacteriana(s) simples com as células ressuspensas de h. meleagridis de modo uma obter uma cultura de cepa bacteriana simples de h. meleagridis. a invenção revela adicionalmente uma formulação de vacina que consiste em um componente de histomonas que consistem em uma cultura atenuada de histomonas meleagridis, um componente bacteriano que consiste em uma ou mais culturas de uma cepa bacteriana simples e compostos de formulação não biológica farmaceuticamente aceitáveis.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12174582.2A EP2682457A1 (en) | 2012-07-02 | 2012-07-02 | Production and application of protozoa cultures of Histomonas meleagridis (H. meleagridis) |
| PCT/EP2013/063889 WO2014006018A1 (en) | 2012-07-02 | 2013-07-02 | Production and application of protozoa cultures of histomonas meleagridis (h. meleagridis) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112014032937A2 true BR112014032937A2 (pt) | 2017-08-01 |
Family
ID=48793189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014032937A BR112014032937A2 (pt) | 2012-07-02 | 2013-07-02 | produção e aplicação de culturas de protozoários de histomonas meleagridis (h. meleagridis) |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US9833501B2 (pt) |
| EP (2) | EP2682457A1 (pt) |
| JP (2) | JP6386452B2 (pt) |
| CN (1) | CN104471054A (pt) |
| AU (1) | AU2013286002B2 (pt) |
| BR (1) | BR112014032937A2 (pt) |
| CA (1) | CA2877539A1 (pt) |
| CL (1) | CL2014003549A1 (pt) |
| ES (1) | ES2893834T3 (pt) |
| MX (1) | MX363037B (pt) |
| WO (1) | WO2014006018A1 (pt) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633843B (zh) * | 2018-04-18 | 2020-10-27 | 中国科学院水生生物研究所 | 一种鲩肠袋虫的体外培养方法及使用的培养基的制备方法 |
| CN110616149A (zh) * | 2019-10-11 | 2019-12-27 | 扬州大学 | 一种鸡源火鸡组织滴虫传代致弱株、其制备方法及用途 |
| CN112322765B (zh) * | 2020-10-15 | 2022-11-22 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | 快速检测鸡球虫与组织滴虫的特异性引物、试剂盒及方法 |
| EP4112717A1 (en) | 2021-06-29 | 2023-01-04 | Veterinärmedizinische Universität Wien | Attenuated histomonas meleagridis strain and vaccine |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT502702B1 (de) | 2005-05-12 | 2008-05-15 | Veterinaermedizinische Uni Wie | Verfahren zur herstellung und anwendung von protozoenkulturen |
-
2012
- 2012-07-02 EP EP12174582.2A patent/EP2682457A1/en not_active Withdrawn
-
2013
- 2013-07-02 AU AU2013286002A patent/AU2013286002B2/en not_active Ceased
- 2013-07-02 MX MX2014016016A patent/MX363037B/es unknown
- 2013-07-02 CA CA2877539A patent/CA2877539A1/en not_active Abandoned
- 2013-07-02 EP EP13737171.2A patent/EP2867356B1/en active Active
- 2013-07-02 ES ES13737171T patent/ES2893834T3/es active Active
- 2013-07-02 US US14/412,582 patent/US9833501B2/en active Active
- 2013-07-02 CN CN201380035336.5A patent/CN104471054A/zh active Pending
- 2013-07-02 BR BR112014032937A patent/BR112014032937A2/pt not_active Application Discontinuation
- 2013-07-02 JP JP2015519185A patent/JP6386452B2/ja not_active Expired - Fee Related
- 2013-07-02 WO PCT/EP2013/063889 patent/WO2014006018A1/en active Application Filing
-
2014
- 2014-12-29 CL CL2014003549A patent/CL2014003549A1/es unknown
-
2017
- 2017-11-02 US US15/802,350 patent/US10350281B2/en active Active
-
2018
- 2018-04-19 JP JP2018080692A patent/JP2018148893A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2013286002B2 (en) | 2019-01-31 |
| CL2014003549A1 (es) | 2015-08-07 |
| CN104471054A (zh) | 2015-03-25 |
| EP2867356B1 (en) | 2021-08-18 |
| EP2867356A1 (en) | 2015-05-06 |
| EP2682457A1 (en) | 2014-01-08 |
| MX2014016016A (es) | 2015-12-07 |
| AU2013286002A1 (en) | 2015-01-22 |
| CA2877539A1 (en) | 2014-01-09 |
| US20150157698A1 (en) | 2015-06-11 |
| US20180117131A1 (en) | 2018-05-03 |
| US9833501B2 (en) | 2017-12-05 |
| ES2893834T3 (es) | 2022-02-10 |
| JP2015522269A (ja) | 2015-08-06 |
| US10350281B2 (en) | 2019-07-16 |
| MX363037B (es) | 2019-03-01 |
| JP2018148893A (ja) | 2018-09-27 |
| WO2014006018A1 (en) | 2014-01-09 |
| JP6386452B2 (ja) | 2018-09-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
|
| B07G | Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette] |
Free format text: NOTIFICACAO DE DEVOLUCAO DO PEDIDO POR NAO SE ENQUADRAR NO ART. 229-C DA LPI. |
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| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |