MX342055B - Iniciadores y sondas de kras. - Google Patents

Iniciadores y sondas de kras.

Info

Publication number
MX342055B
MX342055B MX2012011698A MX2012011698A MX342055B MX 342055 B MX342055 B MX 342055B MX 2012011698 A MX2012011698 A MX 2012011698A MX 2012011698 A MX2012011698 A MX 2012011698A MX 342055 B MX342055 B MX 342055B
Authority
MX
Mexico
Prior art keywords
probes
mutation
oligonucleotide primers
present
kras gene
Prior art date
Application number
MX2012011698A
Other languages
English (en)
Other versions
MX2012011698A (es
Inventor
Stephens Craig
Original Assignee
Response Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Response Genetics Inc filed Critical Response Genetics Inc
Publication of MX2012011698A publication Critical patent/MX2012011698A/es
Publication of MX342055B publication Critical patent/MX342055B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención proporciona iniciadores o sondas de oligonucleótidos para la detección de una mutación del gen KRAS. La invención proporciona además un método para detectar una mutación en el gen KRAS usando los iniciadores o sondas de oligonucleótidos descritas en la misma. Además, la presente invención abarca un método para predecir la sensibilidad de un tumor en un paciente a la quimioterapia dirigida al receptor del factor de crecimiento epidérmico, que comprende obtener ADN del tumor; y determinar si hay una mutación en el codón 12 y/o una mutación en el codón 13 en el exón 2 del gen KRAS en el ADN usando un método que utiliza al menos uno de los iniciadores y/o sondas de oligonucleótidos de la presente invención.
MX2012011698A 2010-04-12 2011-04-12 Iniciadores y sondas de kras. MX342055B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32311410P 2010-04-12 2010-04-12
PCT/US2011/032108 WO2011130265A2 (en) 2010-04-12 2011-04-12 Kras primers and probes

Publications (2)

Publication Number Publication Date
MX2012011698A MX2012011698A (es) 2013-03-20
MX342055B true MX342055B (es) 2016-09-12

Family

ID=44588159

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012011698A MX342055B (es) 2010-04-12 2011-04-12 Iniciadores y sondas de kras.

Country Status (12)

Country Link
US (2) US20130029336A1 (es)
EP (1) EP2558595A2 (es)
JP (1) JP2013523178A (es)
KR (1) KR20120140252A (es)
CN (1) CN102869790B (es)
AU (1) AU2011240653A1 (es)
CA (1) CA2796281C (es)
HK (1) HK1180726A1 (es)
IL (1) IL222379A0 (es)
MX (1) MX342055B (es)
NZ (1) NZ602920A (es)
WO (1) WO2011130265A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102888466A (zh) * 2012-10-30 2013-01-23 武汉友芝友生物制药有限公司 Kras基因突变检测试剂盒及检测方法
US10266828B2 (en) 2013-12-16 2019-04-23 Syddansk Universitet RAS exon 2 skipping for cancer treatment
KR102600344B1 (ko) * 2015-07-17 2023-11-09 주식회사 젠큐릭스 Kras 유전자 돌연변이 검출용 조성물 및 이를 포함하는 키트
CN106282363A (zh) * 2016-08-31 2017-01-04 北京晋祺生物科技有限公司 一种kras基因的检测引物组、其构成的反应体系及应用
CN111500727A (zh) * 2020-04-30 2020-08-07 北京和合医学诊断技术股份有限公司 用于检测kras基因和braf基因突变的引物组及其应用方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
CA2260362A1 (en) * 1996-07-11 1998-01-22 Takanori Oka Nucleic acid assay process and assay kit
GB9715034D0 (en) * 1997-07-18 1997-09-24 Zeneca Ltd Assay
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
ES2370054T3 (es) * 2005-08-24 2011-12-12 Bristol-Myers Squibb Company Biomarcadores y procedimientos para determinar la sensibilidad a moduladores del receptor del factor de crecimiento epidérmico.
EP2004666B1 (en) * 2006-03-27 2014-09-03 Globeimmune, Inc. Ras mutation and compositions and methods related thereto
EP2465950B1 (en) * 2007-03-13 2017-07-12 Amgen Inc. K-ras mutations and anti-egfr antibody therapy
US20090075267A1 (en) * 2007-03-13 2009-03-19 Salvatore Siena K-ras and B-raf mutations and anti-EGFr antibody therapy

Also Published As

Publication number Publication date
CN102869790B (zh) 2014-11-26
WO2011130265A3 (en) 2012-05-31
MX2012011698A (es) 2013-03-20
JP2013523178A (ja) 2013-06-17
WO2011130265A2 (en) 2011-10-20
CA2796281A1 (en) 2011-10-20
US20130029336A1 (en) 2013-01-31
IL222379A0 (en) 2012-12-31
HK1180726A1 (en) 2013-10-25
US20150184250A1 (en) 2015-07-02
KR20120140252A (ko) 2012-12-28
AU2011240653A1 (en) 2012-11-01
CA2796281C (en) 2016-10-11
NZ602920A (en) 2014-07-25
EP2558595A2 (en) 2013-02-20
CN102869790A (zh) 2013-01-09

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