MX337137B - Anticuerpos monoclonales y metodos de deteccion de enzimas que confieren resistencia a acido 2, 4-diclorofenoxiacetico. - Google Patents

Anticuerpos monoclonales y metodos de deteccion de enzimas que confieren resistencia a acido 2, 4-diclorofenoxiacetico.

Info

Publication number
MX337137B
MX337137B MX2013002867A MX2013002867A MX337137B MX 337137 B MX337137 B MX 337137B MX 2013002867 A MX2013002867 A MX 2013002867A MX 2013002867 A MX2013002867 A MX 2013002867A MX 337137 B MX337137 B MX 337137B
Authority
MX
Mexico
Prior art keywords
monoclonal antibodies
enzymes
detection methods
confer resistance
dichlorophenoxyacetic acid
Prior art date
Application number
MX2013002867A
Other languages
English (en)
Other versions
MX2013002867A (es
Inventor
Guomin Shan
Gaofeng Lin
Shawna K Embrey
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2013002867A publication Critical patent/MX2013002867A/es
Publication of MX337137B publication Critical patent/MX337137B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un anticuerpo monoclonal que se une específicamente a una enzima dioxigenasa de ariloxialcanoato (AAD-1) producido por un hibridoma depositado en la Americam Type Culture Collection (colección de cultivos tipo americano (ATCC) bajo el número de acceso seleccionado del grupo que consiste de PTA-11251 (47F185.#18), PTAQ-11252 (473F225.2#7), PTA-11253 (73F27.2#36), PTA-11254 (473H46.2) y PTA 11255 (473H172.3). Un método para identificar la presencia de una enzima AAD-1 que comprende: a) inmovilizar un primer anticuerpo monoclonal sobre una superficie de ensayo después lavar dicha superficie de ensayo; b) poner en contacto dicha superficie con un líquido de ensayo sospechoso de contener AAD-1, durante un periodo de tiempo suficiente para permitir el enlace y luego lavar dicha superficie de ensayo; c) poner en contacto dicha superficie de ensayo con un segundo anticuerpo diferente, conjugado con un grupo de reporte durante un período de tiempo suficiente para permitir el enlace de dicho segundo anticuerpo monoclonal conjugado y a continuación lavar dicha superficie de ensayo, y, d) detectar la presencia o ausencia de dicho grupo de reporte.
MX2013002867A 2010-09-15 2011-08-26 Anticuerpos monoclonales y metodos de deteccion de enzimas que confieren resistencia a acido 2, 4-diclorofenoxiacetico. MX337137B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38301510P 2010-09-15 2010-09-15
PCT/US2011/049263 WO2012036870A2 (en) 2010-09-15 2011-08-26 Monoclonal antibodies and detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid

Publications (2)

Publication Number Publication Date
MX2013002867A MX2013002867A (es) 2013-06-28
MX337137B true MX337137B (es) 2016-01-22

Family

ID=45807077

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013002867A MX337137B (es) 2010-09-15 2011-08-26 Anticuerpos monoclonales y metodos de deteccion de enzimas que confieren resistencia a acido 2, 4-diclorofenoxiacetico.

Country Status (13)

Country Link
US (1) US8673583B2 (es)
EP (1) EP2616488B1 (es)
JP (1) JP5940538B2 (es)
KR (1) KR101934542B1 (es)
CN (1) CN103209993B (es)
AR (1) AR082983A1 (es)
AU (1) AU2011302508B2 (es)
BR (1) BR112013006146B8 (es)
CA (1) CA2810390C (es)
CL (1) CL2013000697A1 (es)
DK (1) DK2616488T3 (es)
MX (1) MX337137B (es)
WO (1) WO2012036870A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2576623B1 (en) 2010-06-04 2015-01-21 Dow AgroSciences LLC Monoclonal antibodies detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid in plants
US9738705B2 (en) * 2014-04-29 2017-08-22 Dow Agrosciences Llc Monoclonal antibodies specific for Cry1Ca and related detection methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4363799A (en) 1979-03-20 1982-12-14 Ortho Pharmaceutical Corporation Monoclonal antibody to human T cells, and methods for preparing same
DE3629890A1 (de) 1986-08-29 1988-03-10 Schering Ag Mikroorganismen und plasmide fuer die 2,4-dichlorphenoxyessigsaeure (2,4-d)-monooxigenase - bildung und verfahren zur herstellung dieser plasmide und staemme
AU5482299A (en) * 1998-08-12 2000-03-06 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
MXPA01010921A (es) * 1999-04-29 2003-06-24 Syngenta Ltd Plantas resistentes a herbicidas.
BR122013026754B1 (pt) * 2000-06-22 2018-02-27 Monsanto Company Molécula de dna e processos para produzir uma planta de milho tolerante à aplicação do herbicida glifosato
CA2427816A1 (en) * 2000-11-03 2002-05-16 Dow Agrosciences Llc N-(¢1,2,4!triazoloazinyl) thiophenesulfonamide compounds as herbicides
PT2298901E (pt) * 2004-04-30 2013-05-15 Dow Agrosciences Llc Novos genes de resistência a herbicida
BRPI0618025B1 (pt) * 2005-10-28 2016-12-27 Dow Agrosciences Llc método para controlar ervas daninhas em uma área, polinucleotídeo isolado, célula de planta e planta resistente a herbicida
BRPI0811294B8 (pt) * 2007-05-09 2022-06-28 Dow Agrosciences Llc Polinucleotídeo isolado, e método para controle de ervas daninhas em uma área
US8598413B2 (en) * 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
BR112012003884A2 (pt) * 2009-08-19 2015-09-01 Dow Agrosciences Llc Evento aad-1 das-40278-9, linhagens de milho trangênico relacionadas e identificação evento-específica das mesmas.
JP5939989B2 (ja) * 2010-01-22 2016-06-29 ダウ アグロサイエンシィズ エルエルシー 植物における遺伝子ターゲティング用の操作されたランディングパッド
EP2576623B1 (en) 2010-06-04 2015-01-21 Dow AgroSciences LLC Monoclonal antibodies detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid in plants

Also Published As

Publication number Publication date
BR112013006146B1 (pt) 2020-04-14
MX2013002867A (es) 2013-06-28
WO2012036870A2 (en) 2012-03-22
US8673583B2 (en) 2014-03-18
CN103209993B (zh) 2014-12-10
CL2013000697A1 (es) 2014-07-25
EP2616488A2 (en) 2013-07-24
AU2011302508B2 (en) 2016-09-29
WO2012036870A3 (en) 2012-07-19
CA2810390A1 (en) 2012-03-22
EP2616488A4 (en) 2014-02-12
CA2810390C (en) 2018-10-23
BR112013006146B8 (pt) 2022-10-11
AU2011302508A1 (en) 2013-03-21
US20120064542A1 (en) 2012-03-15
AR082983A1 (es) 2013-01-23
CN103209993A (zh) 2013-07-17
EP2616488B1 (en) 2015-03-04
DK2616488T3 (en) 2015-05-26
KR20130107299A (ko) 2013-10-01
BR112013006146A2 (pt) 2016-06-14
JP5940538B2 (ja) 2016-06-29
JP2013541525A (ja) 2013-11-14
KR101934542B1 (ko) 2019-01-03

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