MX2023002103A - Metodos y aparatos para la purificacion de goticulas de gel de soporte de tejido biologico. - Google Patents

Metodos y aparatos para la purificacion de goticulas de gel de soporte de tejido biologico.

Info

Publication number
MX2023002103A
MX2023002103A MX2023002103A MX2023002103A MX2023002103A MX 2023002103 A MX2023002103 A MX 2023002103A MX 2023002103 A MX2023002103 A MX 2023002103A MX 2023002103 A MX2023002103 A MX 2023002103A MX 2023002103 A MX2023002103 A MX 2023002103A
Authority
MX
Mexico
Prior art keywords
apparatuses
gel droplets
methods
biological tissue
purification
Prior art date
Application number
MX2023002103A
Other languages
English (en)
Inventor
Xiling Shen
Zhaohui Wang
Original Assignee
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke filed Critical Univ Duke
Publication of MX2023002103A publication Critical patent/MX2023002103A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0202—Separation of non-miscible liquids by ab- or adsorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/08—Thickening liquid suspensions by filtration
    • B01D17/085—Thickening liquid suspensions by filtration with membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/02—Details relating to pores or porosity of the membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/02—Details relating to pores or porosity of the membranes
    • B01D2325/0283—Pore size
    • B01D2325/02834—Pore size more than 0.1 and up to 1 µm
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/38—Hydrophobic membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Thermal Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

En la presente se describen métodos y aparatos para formar gotículas de gel que incluyen tejido biológico (por ejemplo, células) y, en particular, métodos y aparatos para eliminar aceite de las gotículas de gel que comprenden células disociadas (incluidas microorganosferas). Aunque es beneficioso usar aceite en la formación de estas gotículas de gel, y en particular microorganosferas, el aceite puede inhibir el crecimiento y la supervivencia de las células dentro de las gotículas de gel. Los métodos y aparatos descritos en la presente pueden permitir la eliminación de aceite y pueden mejorar la supervivencia y la calidad de las gotículas de gel resultantes.
MX2023002103A 2020-08-26 2021-08-26 Metodos y aparatos para la purificacion de goticulas de gel de soporte de tejido biologico. MX2023002103A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063070334P 2020-08-26 2020-08-26
US17/233,950 US11628382B2 (en) 2020-08-26 2021-04-19 Methods and apparatuses for purification of gel droplets supporting biological tissue
PCT/US2021/047634 WO2022046958A1 (en) 2020-08-26 2021-08-26 Methods and apparatuses for purification of gel droplets supporting biological tissue

Publications (1)

Publication Number Publication Date
MX2023002103A true MX2023002103A (es) 2023-03-15

Family

ID=80353937

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023002103A MX2023002103A (es) 2020-08-26 2021-08-26 Metodos y aparatos para la purificacion de goticulas de gel de soporte de tejido biologico.

Country Status (9)

Country Link
US (2) US11628382B2 (es)
EP (1) EP4168573A1 (es)
JP (1) JP2023539242A (es)
KR (1) KR20230057347A (es)
CN (1) CN115917005A (es)
AU (1) AU2021332279A1 (es)
CA (1) CA3189614A1 (es)
MX (1) MX2023002103A (es)
WO (1) WO2022046958A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11466240B2 (en) * 2017-07-14 2022-10-11 The Governing Council Of The University Of Toronto Microfluidic platform for the rapid production of organoids/spheroids for compound screening
CN114702688B (zh) * 2022-03-13 2023-12-12 浙江大学 一种离心式水凝胶液滴的制备方法
WO2023212732A2 (en) * 2022-04-29 2023-11-02 Xilis, Inc. Methods and apparatuses for testing hepatocyte toxicity using microorganospheres
WO2024040145A1 (en) * 2022-08-17 2024-02-22 Xilis, Inc. Methods of delivering components to microorganospheres
US20240118187A1 (en) 2022-10-11 2024-04-11 Xilis, Inc. Measurement and control of droplet sizes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5266480A (en) 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US20160022873A1 (en) 2013-03-14 2016-01-28 Research Institute At Nationwide Children's Hospital, Inc. Tissue engineered intestine
WO2016145242A1 (en) 2015-03-11 2016-09-15 Agenus Inc. Methods and compositions for high throughput screening of biomolecules using gel microdrops
WO2017036533A1 (en) 2015-09-03 2017-03-09 Ecole Polytechnique Federale De Lausanne (Epfl) Three-dimensional hydrogels for culturing adult epithelial stem cells and organoids
IL266318B2 (en) 2016-11-08 2023-10-01 Nanna Therapeutics Ltd A chemical library without coded labeling

Also Published As

Publication number Publication date
WO2022046958A1 (en) 2022-03-03
AU2021332279A1 (en) 2023-02-16
US20220062791A1 (en) 2022-03-03
US20230364528A1 (en) 2023-11-16
US11628382B2 (en) 2023-04-18
EP4168573A1 (en) 2023-04-26
KR20230057347A (ko) 2023-04-28
CA3189614A1 (en) 2022-03-03
CN115917005A (zh) 2023-04-04
JP2023539242A (ja) 2023-09-13

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