MX2022012342A - Metodo para producir hialuronidasa recombinante. - Google Patents
Metodo para producir hialuronidasa recombinante.Info
- Publication number
- MX2022012342A MX2022012342A MX2022012342A MX2022012342A MX2022012342A MX 2022012342 A MX2022012342 A MX 2022012342A MX 2022012342 A MX2022012342 A MX 2022012342A MX 2022012342 A MX2022012342 A MX 2022012342A MX 2022012342 A MX2022012342 A MX 2022012342A
- Authority
- MX
- Mexico
- Prior art keywords
- producing recombinant
- recombinant hyaluronidase
- hyaluronidase
- culturing
- producing
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 108010003272 Hyaluronate lyase Proteins 0.000 title abstract 2
- 102000001974 Hyaluronidases Human genes 0.000 title abstract 2
- 229960002773 hyaluronidase Drugs 0.000 title abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2474—Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01035—Hyaluronoglucosaminidase (3.2.1.35), i.e. hyaluronidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se divulga un método para producir hialuronidasa o una variante de la misma. Específicamente, el método es capaz de cambiar los niveles de N-glicano bajo condiciones de cultivo que incluyen una concentración controlada de glucosa en el medio de cultivo y una temperatura de cultivo disminuida durante un periodo de tiempo de cultivo específico, aumentando así la actividad específica en un 10% o más y mejorando la calidad y el rendimiento de la producción.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20200099100 | 2020-08-07 | ||
| PCT/KR2021/010368 WO2022031093A1 (ko) | 2020-08-07 | 2021-08-06 | 재조합 히알루로니다제의 생산 방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2022012342A true MX2022012342A (es) | 2022-11-16 |
Family
ID=80118320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2022012342A MX2022012342A (es) | 2020-08-07 | 2021-08-06 | Metodo para producir hialuronidasa recombinante. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20230174963A1 (es) |
| EP (1) | EP4194551A1 (es) |
| JP (1) | JP2023528142A (es) |
| KR (2) | KR102649603B1 (es) |
| CN (1) | CN115427562A (es) |
| AU (1) | AU2021320569A1 (es) |
| BR (1) | BR112022019726A2 (es) |
| CA (1) | CA3173310A1 (es) |
| MX (1) | MX2022012342A (es) |
| WO (1) | WO2022031093A1 (es) |
| ZA (1) | ZA202210840B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024005502A1 (ko) * | 2022-06-29 | 2024-01-04 | 주식회사 오디스젠 | 중성 ph에서 활성을 나타내는 히알루로니다제 hyal1 변이체 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4464395B2 (ja) | 2003-03-05 | 2010-05-19 | ヘイローザイム インコーポレイテッド | 可溶性ヒアルロニダーゼ糖タンパク質（ｓＨＡＳＥＧＰ）、その調製プロセス、使用およびそれを含む薬学的組成物 |
| KR20140130512A (ko) * | 2008-03-06 | 2014-11-10 | 할로자임, 아이엔씨 | 가용성 히알루로니다아제의 대규모 제조 |
| EP2285402A2 (en) * | 2008-04-14 | 2011-02-23 | Halozyme, Inc. | Modified hyaluronidases and uses in treating hyaluronan-associated diseases and conditions |
| DK3037529T3 (da) * | 2008-12-09 | 2019-05-20 | Halozyme Inc | Udvidede opløselige ph20-polypeptider og anvendelse deraf |
| IL298330A (en) | 2011-12-30 | 2023-01-01 | Halozyme Inc | Ph20 polypeptide variants, formulations and uses thereof |
| JP7204729B2 (ja) * | 2018-07-25 | 2023-01-16 | アルテオゼン インコーポレイテッド | 新規なヒアルロン酸加水分解酵素変異体およびそれを含む薬学組成物 |
-
2021
- 2021-08-06 AU AU2021320569A patent/AU2021320569A1/en active Pending
- 2021-08-06 WO PCT/KR2021/010368 patent/WO2022031093A1/ko unknown
- 2021-08-06 KR KR1020210103530A patent/KR102649603B1/ko active Application Filing
- 2021-08-06 MX MX2022012342A patent/MX2022012342A/es unknown
- 2021-08-06 EP EP21853474.1A patent/EP4194551A1/en active Pending
- 2021-08-06 BR BR112022019726A patent/BR112022019726A2/pt unknown
- 2021-08-06 US US17/907,538 patent/US20230174963A1/en active Pending
- 2021-08-06 JP JP2022559471A patent/JP2023528142A/ja active Pending
- 2021-08-06 CN CN202180030097.9A patent/CN115427562A/zh active Pending
- 2021-08-06 CA CA3173310A patent/CA3173310A1/en active Pending
-
2022
- 2022-09-30 ZA ZA2022/10840A patent/ZA202210840B/en unknown
-
2024
- 2024-03-15 KR KR1020240036308A patent/KR20240037935A/ko active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| KR102649603B1 (ko) | 2024-03-21 |
| AU2021320569A1 (en) | 2022-10-27 |
| EP4194551A1 (en) | 2023-06-14 |
| CN115427562A (zh) | 2022-12-02 |
| CA3173310A1 (en) | 2022-02-10 |
| BR112022019726A2 (pt) | 2023-03-07 |
| JP2023528142A (ja) | 2023-07-04 |
| WO2022031093A1 (ko) | 2022-02-10 |
| KR20220018943A (ko) | 2022-02-15 |
| US20230174963A1 (en) | 2023-06-08 |
| KR20240037935A (ko) | 2024-03-22 |
| ZA202210840B (en) | 2023-08-30 |
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