MX2022009917A - Aparato para mejorar la eficiencia de transfeccion y/o administracion intracelular de un agente en una celula eucariota y/o la expresion de proteinas y metodo de uso del mismo. - Google Patents

Aparato para mejorar la eficiencia de transfeccion y/o administracion intracelular de un agente en una celula eucariota y/o la expresion de proteinas y metodo de uso del mismo.

Info

Publication number
MX2022009917A
MX2022009917A MX2022009917A MX2022009917A MX2022009917A MX 2022009917 A MX2022009917 A MX 2022009917A MX 2022009917 A MX2022009917 A MX 2022009917A MX 2022009917 A MX2022009917 A MX 2022009917A MX 2022009917 A MX2022009917 A MX 2022009917A
Authority
MX
Mexico
Prior art keywords
mixture
transfection
agent
protein expression
eukaryotic cell
Prior art date
Application number
MX2022009917A
Other languages
English (en)
Inventor
Henry William J
Anna Montali
Jean-Christophe Bourdon
Original Assignee
St Andrews Pharmaceutical Tech Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2004412.9A external-priority patent/GB202004412D0/en
Priority claimed from GBGB2004411.1A external-priority patent/GB202004411D0/en
Priority claimed from GBGB2009296.1A external-priority patent/GB202009296D0/en
Priority claimed from GBGB2009297.9A external-priority patent/GB202009297D0/en
Application filed by St Andrews Pharmaceutical Tech Limited filed Critical St Andrews Pharmaceutical Tech Limited
Publication of MX2022009917A publication Critical patent/MX2022009917A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/0624—Apparatus adapted for a specific treatment for eliminating microbes, germs, bacteria on or in the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N2/00—Magnetotherapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N2005/0635—Radiation therapy using light characterised by the body area to be irradiated
    • A61N2005/0643—Applicators, probes irradiating specific body areas in close proximity
    • A61N2005/0645—Applicators worn by the patient
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0601—Apparatus for use inside the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporciona un método y un aparato para mejorar la eficiencia de la transfección y/o un proceso de administración intracelular en una o más células eucariotas. El método incluye las etapas de proporcionar al menos un agente desnudo adecuado para transfección y/o administración intracelular. Introducir el al menos un agente desnudo en una o más células eucariotas para formar una mezcla o mezcla de transfección y permitir que la mezcla o mezcla de transfección experimente un proceso de transfección o un proceso de administración intracelular para formar una o más células eucariotas transfectadas o tratadas. El método incluye también la etapa de dirigir señales electromagnéticas pulsadas proporcionadas en cualquiera o cualquier combinación de una frecuencia predeterminada, a una tasa de pulso predeterminada, o a una potencia predeterminada, en el al menos un agente desnudo en la etapa a) antes de crear la mezcla o mezcla de transfección, en la mezcla o mezcla de transfección en la etapa b), en la mezcla o mezcla de transfección en la etapa c) y/o en la mezcla de células transfectadas o tratadas después de la etapa c).
MX2022009917A 2020-03-26 2021-03-25 Aparato para mejorar la eficiencia de transfeccion y/o administracion intracelular de un agente en una celula eucariota y/o la expresion de proteinas y metodo de uso del mismo. MX2022009917A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB2004412.9A GB202004412D0 (en) 2020-03-26 2020-03-26 Method and apparatus for improvements to gene therapy
GBGB2004411.1A GB202004411D0 (en) 2020-03-26 2020-03-26 Apparatus and method for the application of electromagnetic signals for anti-viral transdermal and/or treatment of a medical condition
GBGB2009296.1A GB202009296D0 (en) 2020-06-18 2020-06-18 Apparatus and method for the application of electromagnetic signals for anti-viral, transdermal and/or direct treatment of a medical condition
GBGB2009297.9A GB202009297D0 (en) 2020-06-18 2020-06-18 Method and apparatus for improvements to gene therapy
PCT/GB2021/050736 WO2021191623A1 (en) 2020-03-26 2021-03-25 Apparatus for improved transfection and/or intracellular delivery efficiency of an agent into a eukaryotic cell and/or protein expression and method of use thereof

Publications (1)

Publication Number Publication Date
MX2022009917A true MX2022009917A (es) 2022-09-09

Family

ID=75377829

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2022009917A MX2022009917A (es) 2020-03-26 2021-03-25 Aparato para mejorar la eficiencia de transfeccion y/o administracion intracelular de un agente en una celula eucariota y/o la expresion de proteinas y metodo de uso del mismo.
MX2022009916A MX2022009916A (es) 2020-03-26 2021-03-25 Aparato para mejorar la eficiencia de transfeccion y/o expresion de proteinas y metodo de uso del mismo.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2022009916A MX2022009916A (es) 2020-03-26 2021-03-25 Aparato para mejorar la eficiencia de transfeccion y/o expresion de proteinas y metodo de uso del mismo.

Country Status (14)

Country Link
US (2) US20230151386A1 (es)
EP (2) EP4058134A1 (es)
JP (2) JP2023519317A (es)
KR (2) KR20220157375A (es)
CN (2) CN115315291A (es)
AU (2) AU2021245088A1 (es)
BR (2) BR112022017417A2 (es)
CA (2) CA3163155A1 (es)
CL (2) CL2022002577A1 (es)
GB (2) GB2606942A (es)
IL (2) IL296677A (es)
MX (2) MX2022009917A (es)
WO (2) WO2021191623A1 (es)
ZA (2) ZA202210031B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024025825A1 (en) * 2022-07-25 2024-02-01 Mayo Foundation For Medical Education And Research Bioreactor systems and methods for electrically stimulating cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6152882A (en) * 1999-01-26 2000-11-28 Impulse Dynamics N.V. Apparatus and method for chronic measurement of monophasic action potentials
WO2016003485A1 (en) * 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
US11547848B2 (en) * 2018-06-21 2023-01-10 Regenesis Biomedical, Inc. High-power pulsed electromagnetic field applicator systems

Also Published As

Publication number Publication date
MX2022009916A (es) 2022-09-09
EP4058134A1 (en) 2022-09-21
CA3163153A1 (en) 2021-09-30
KR20220157375A (ko) 2022-11-29
US20230151386A1 (en) 2023-05-18
GB2606943A (en) 2022-11-23
CN115315291A (zh) 2022-11-08
US20230159954A1 (en) 2023-05-25
AU2021242028A1 (en) 2022-07-14
CL2022002577A1 (es) 2023-07-07
EP4058135A1 (en) 2022-09-21
KR20220157941A (ko) 2022-11-29
JP2023519317A (ja) 2023-05-10
JP2023519316A (ja) 2023-05-10
CA3163155A1 (en) 2021-09-30
IL296677A (en) 2022-11-01
BR112022017859A2 (pt) 2023-02-28
ZA202210031B (en) 2023-04-26
WO2021191624A1 (en) 2021-09-30
CL2022002575A1 (es) 2023-04-21
ZA202210033B (en) 2023-04-26
WO2021191623A1 (en) 2021-09-30
CN115361996A (zh) 2022-11-18
GB2606942A (en) 2022-11-23
IL296674A (en) 2022-11-01
GB202210608D0 (en) 2022-08-31
AU2021245088A1 (en) 2022-07-14
BR112022017417A2 (pt) 2022-11-22
GB202210609D0 (en) 2022-08-31

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