BR112022017859A2 - Aparelho para eficiência de transfecção e/ou entrega intracelular melhorada de um agente em uma célula eucariótica e/ou expressão de proteínas e método de uso do mesmo - Google Patents

Aparelho para eficiência de transfecção e/ou entrega intracelular melhorada de um agente em uma célula eucariótica e/ou expressão de proteínas e método de uso do mesmo

Info

Publication number
BR112022017859A2
BR112022017859A2 BR112022017859A BR112022017859A BR112022017859A2 BR 112022017859 A2 BR112022017859 A2 BR 112022017859A2 BR 112022017859 A BR112022017859 A BR 112022017859A BR 112022017859 A BR112022017859 A BR 112022017859A BR 112022017859 A2 BR112022017859 A2 BR 112022017859A2
Authority
BR
Brazil
Prior art keywords
mixture
transfection
intracellular delivery
agent
proteins
Prior art date
Application number
BR112022017859A
Other languages
English (en)
Inventor
Montali Anna
j henry William
Bourdon Jean-Cristophe
Original Assignee
St Andrews Pharmaceutical Tech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2004411.1A external-priority patent/GB202004411D0/en
Priority claimed from GBGB2004412.9A external-priority patent/GB202004412D0/en
Priority claimed from GBGB2009297.9A external-priority patent/GB202009297D0/en
Priority claimed from GBGB2009296.1A external-priority patent/GB202009296D0/en
Application filed by St Andrews Pharmaceutical Tech Ltd filed Critical St Andrews Pharmaceutical Tech Ltd
Publication of BR112022017859A2 publication Critical patent/BR112022017859A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N2/00—Magnetotherapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/0624—Apparatus adapted for a specific treatment for eliminating microbes, germs, bacteria on or in the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N2005/0635—Radiation therapy using light characterised by the body area to be irradiated
    • A61N2005/0643—Applicators, probes irradiating specific body areas in close proximity
    • A61N2005/0645—Applicators worn by the patient
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0601—Apparatus for use inside the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Aparelho para Eficiência de Transfecção e/ou Entrega Intracelular Melhorada de um Agente em uma Célula Eucariótica e/ou Expressão de Proteínas e Método de Uso do Mesmo. Um método e aparelho para melhorar a eficiência de transfecção e/ou um processo de entrega intracelular em uma ou mais células eucarióticas são proporcionados. O método inclui as etapas de proporcionar pelo menos um agente nu adequado para transfecção e/ou entrega intracelular. Introduzir o pelo menos um agente nu em uma ou mais células eucarióticas para formar uma mistura ou mistura de transfecção e permitir que a mistura ou mistura de transfecção seja submetida a um processo de transfecção ou processo de entrega intracelular para formar uma ou mais células eucarióticas tratadas ou transfectadas. O método também inclui a etapa de direcionar os sinais eletromagnéticos pulsados fornecidos em qualquer ou qualquer combinação de uma frequência predeterminada, a uma taxa de pulso predeterminada ou a uma potência predeterminada, ao pelo menos um agente nu na etapa a) antes de criar a mistura ou mistura de transfecção, à mistura ou mistura de transfecção na etapa b), à mistura ou mistura de transfecção na etapa c) e/ou à mistura de células tratadas ou transfectadas após a etapa c).
BR112022017859A 2020-03-26 2021-03-25 Aparelho para eficiência de transfecção e/ou entrega intracelular melhorada de um agente em uma célula eucariótica e/ou expressão de proteínas e método de uso do mesmo BR112022017859A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB2004411.1A GB202004411D0 (en) 2020-03-26 2020-03-26 Apparatus and method for the application of electromagnetic signals for anti-viral transdermal and/or treatment of a medical condition
GBGB2004412.9A GB202004412D0 (en) 2020-03-26 2020-03-26 Method and apparatus for improvements to gene therapy
GBGB2009297.9A GB202009297D0 (en) 2020-06-18 2020-06-18 Method and apparatus for improvements to gene therapy
GBGB2009296.1A GB202009296D0 (en) 2020-06-18 2020-06-18 Apparatus and method for the application of electromagnetic signals for anti-viral, transdermal and/or direct treatment of a medical condition
PCT/GB2021/050736 WO2021191623A1 (en) 2020-03-26 2021-03-25 Apparatus for improved transfection and/or intracellular delivery efficiency of an agent into a eukaryotic cell and/or protein expression and method of use thereof

Publications (1)

Publication Number Publication Date
BR112022017859A2 true BR112022017859A2 (pt) 2023-02-28

Family

ID=75377829

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112022017859A BR112022017859A2 (pt) 2020-03-26 2021-03-25 Aparelho para eficiência de transfecção e/ou entrega intracelular melhorada de um agente em uma célula eucariótica e/ou expressão de proteínas e método de uso do mesmo
BR112022017417A BR112022017417A2 (pt) 2020-03-26 2021-03-25 Aparelho para eficiência de transfecção e/ou expressão de proteínas melhoradas e método de uso do mesmo

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112022017417A BR112022017417A2 (pt) 2020-03-26 2021-03-25 Aparelho para eficiência de transfecção e/ou expressão de proteínas melhoradas e método de uso do mesmo

Country Status (14)

Country Link
US (2) US20230151386A1 (pt)
EP (2) EP4058134A1 (pt)
JP (2) JP2023519316A (pt)
KR (2) KR20220157941A (pt)
CN (2) CN115315291A (pt)
AU (2) AU2021242028A1 (pt)
BR (2) BR112022017859A2 (pt)
CA (2) CA3163153A1 (pt)
CL (2) CL2022002575A1 (pt)
GB (2) GB2606943A (pt)
IL (2) IL296674A (pt)
MX (2) MX2022009917A (pt)
WO (2) WO2021191624A1 (pt)
ZA (2) ZA202210033B (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024025825A1 (en) * 2022-07-25 2024-02-01 Mayo Foundation For Medical Education And Research Bioreactor systems and methods for electrically stimulating cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6152882A (en) * 1999-01-26 2000-11-28 Impulse Dynamics N.V. Apparatus and method for chronic measurement of monophasic action potentials
WO2016003485A1 (en) * 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
US11547848B2 (en) * 2018-06-21 2023-01-10 Regenesis Biomedical, Inc. High-power pulsed electromagnetic field applicator systems

Also Published As

Publication number Publication date
US20230151386A1 (en) 2023-05-18
CN115361996A (zh) 2022-11-18
IL296677A (en) 2022-11-01
AU2021242028A1 (en) 2022-07-14
CL2022002577A1 (es) 2023-07-07
IL296674A (en) 2022-11-01
KR20220157375A (ko) 2022-11-29
EP4058134A1 (en) 2022-09-21
GB2606942A (en) 2022-11-23
CA3163155A1 (en) 2021-09-30
BR112022017417A2 (pt) 2022-11-22
GB202210609D0 (en) 2022-08-31
ZA202210031B (en) 2023-04-26
GB202210608D0 (en) 2022-08-31
WO2021191623A1 (en) 2021-09-30
US20230159954A1 (en) 2023-05-25
CN115315291A (zh) 2022-11-08
JP2023519317A (ja) 2023-05-10
AU2021245088A1 (en) 2022-07-14
WO2021191624A1 (en) 2021-09-30
MX2022009917A (es) 2022-09-09
CL2022002575A1 (es) 2023-04-21
GB2606943A (en) 2022-11-23
EP4058135A1 (en) 2022-09-21
MX2022009916A (es) 2022-09-09
ZA202210033B (en) 2023-04-26
JP2023519316A (ja) 2023-05-10
CA3163153A1 (en) 2021-09-30
KR20220157941A (ko) 2022-11-29

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