CL2022002577A1 - Aparato para eficiencia de transfeccion mejorada y/o expresion de proteina y metodo de uso del mismo. - Google Patents

Aparato para eficiencia de transfeccion mejorada y/o expresion de proteina y metodo de uso del mismo.

Info

Publication number
CL2022002577A1
CL2022002577A1 CL2022002577A CL2022002577A CL2022002577A1 CL 2022002577 A1 CL2022002577 A1 CL 2022002577A1 CL 2022002577 A CL2022002577 A CL 2022002577A CL 2022002577 A CL2022002577 A CL 2022002577A CL 2022002577 A1 CL2022002577 A1 CL 2022002577A1
Authority
CL
Chile
Prior art keywords
protein expression
transfection efficiency
improved transfection
improved
efficiency
Prior art date
Application number
CL2022002577A
Other languages
English (en)
Inventor
William J Henry
Anna Montali
Jean-Christophe Bourdon
Original Assignee
St Andrews Pharmaceutical Tech Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB2004411.1A external-priority patent/GB202004411D0/en
Priority claimed from GBGB2004412.9A external-priority patent/GB202004412D0/en
Priority claimed from GBGB2009297.9A external-priority patent/GB202009297D0/en
Priority claimed from GBGB2009296.1A external-priority patent/GB202009296D0/en
Application filed by St Andrews Pharmaceutical Tech Limited filed Critical St Andrews Pharmaceutical Tech Limited
Publication of CL2022002577A1 publication Critical patent/CL2022002577A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00Radiation therapy
    • A61N5/06Radiation therapy using light
    • A61N5/0613Apparatus adapted for a specific treatment
    • A61N5/0624Apparatus adapted for a specific treatment for eliminating microbes, germs, bacteria on or in the body
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N2/00Magnetotherapy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00Radiation therapy
    • A61N5/06Radiation therapy using light
    • A61N2005/0635Radiation therapy using light characterised by the body area to be irradiated
    • A61N2005/0643Applicators, probes irradiating specific body areas in close proximity
    • A61N2005/0645Applicators worn by the patient
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00Radiation therapy
    • A61N5/06Radiation therapy using light
    • A61N5/0601Apparatus for use inside the body
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00Culture process characterised by the use of electromagnetic stimulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • Sustainable Development (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CL2022002577A 2020-03-26 2022-09-22 Aparato para eficiencia de transfeccion mejorada y/o expresion de proteina y metodo de uso del mismo. CL2022002577A1 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB2004411.1A GB202004411D0 (en) 2020-03-26 2020-03-26 Apparatus and method for the application of electromagnetic signals for anti-viral transdermal and/or treatment of a medical condition
GBGB2004412.9A GB202004412D0 (en) 2020-03-26 2020-03-26 Method and apparatus for improvements to gene therapy
GBGB2009297.9A GB202009297D0 (en) 2020-06-18 2020-06-18 Method and apparatus for improvements to gene therapy
GBGB2009296.1A GB202009296D0 (en) 2020-06-18 2020-06-18 Apparatus and method for the application of electromagnetic signals for anti-viral, transdermal and/or direct treatment of a medical condition

Publications (1)

Publication Number Publication Date
CL2022002577A1 true CL2022002577A1 (es) 2023-07-07

Family

ID=75377829

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2022002575A CL2022002575A1 (es) 2020-03-26 2022-09-22 Aparato para la transfeccion mejorada y/o eficiencia de suministro intracelular de un agente en una celula eucariotica y/o expresion de proteina y metodo de uso de la misma.
CL2022002577A CL2022002577A1 (es) 2020-03-26 2022-09-22 Aparato para eficiencia de transfeccion mejorada y/o expresion de proteina y metodo de uso del mismo.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2022002575A CL2022002575A1 (es) 2020-03-26 2022-09-22 Aparato para la transfeccion mejorada y/o eficiencia de suministro intracelular de un agente en una celula eucariotica y/o expresion de proteina y metodo de uso de la misma.

Country Status (14)

Country Link
US (2) US20230151386A1 (es)
EP (2) EP4058134A1 (es)
JP (2) JP2023519316A (es)
KR (2) KR20220157941A (es)
CN (2) CN115315291A (es)
AU (2) AU2021242028A1 (es)
BR (2) BR112022017859A2 (es)
CA (2) CA3163153A1 (es)
CL (2) CL2022002575A1 (es)
GB (2) GB2606943A (es)
IL (2) IL296674A (es)
MX (2) MX2022009917A (es)
WO (2) WO2021191624A1 (es)
ZA (2) ZA202210033B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024025825A1 (en) * 2022-07-25 2024-02-01 Mayo Foundation For Medical Education And Research Bioreactor systems and methods for electrically stimulating cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6152882A (en) * 1999-01-26 2000-11-28 Impulse Dynamics N.V. Apparatus and method for chronic measurement of monophasic action potentials
WO2016003485A1 (en) * 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
US11547848B2 (en) * 2018-06-21 2023-01-10 Regenesis Biomedical, Inc. High-power pulsed electromagnetic field applicator systems

Also Published As

Publication number Publication date
US20230151386A1 (en) 2023-05-18
CN115361996A (zh) 2022-11-18
IL296677A (en) 2022-11-01
AU2021242028A1 (en) 2022-07-14
IL296674A (en) 2022-11-01
BR112022017859A2 (pt) 2023-02-28
KR20220157375A (ko) 2022-11-29
EP4058134A1 (en) 2022-09-21
GB2606942A (en) 2022-11-23
CA3163155A1 (en) 2021-09-30
BR112022017417A2 (pt) 2022-11-22
GB202210609D0 (en) 2022-08-31
ZA202210031B (en) 2023-04-26
GB202210608D0 (en) 2022-08-31
WO2021191623A1 (en) 2021-09-30
US20230159954A1 (en) 2023-05-25
CN115315291A (zh) 2022-11-08
JP2023519317A (ja) 2023-05-10
AU2021245088A1 (en) 2022-07-14
WO2021191624A1 (en) 2021-09-30
MX2022009917A (es) 2022-09-09
CL2022002575A1 (es) 2023-04-21
GB2606943A (en) 2022-11-23
EP4058135A1 (en) 2022-09-21
MX2022009916A (es) 2022-09-09
ZA202210033B (en) 2023-04-26
JP2023519316A (ja) 2023-05-10
CA3163153A1 (en) 2021-09-30
KR20220157941A (ko) 2022-11-29

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