MX2022003691A - Espectrometria de masas nativa acoplada a cromatografia de interaccion hidrofobica para analisis de anticuerpos. - Google Patents
Espectrometria de masas nativa acoplada a cromatografia de interaccion hidrofobica para analisis de anticuerpos.Info
- Publication number
- MX2022003691A MX2022003691A MX2022003691A MX2022003691A MX2022003691A MX 2022003691 A MX2022003691 A MX 2022003691A MX 2022003691 A MX2022003691 A MX 2022003691A MX 2022003691 A MX2022003691 A MX 2022003691A MX 2022003691 A MX2022003691 A MX 2022003691A
- Authority
- MX
- Mexico
- Prior art keywords
- mass spectrometry
- hydrophobic interaction
- interaction chromatography
- proteins
- peptides
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0036—Step by step routines describing the handling of the data generated during a measurement
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención proporciona métodos y sistemas rápidos, sensibles y de alto rendimiento para caracterizar péptidos o proteínas con espectrometría de masas nativa acoplada a cromatografía de interacción hidrofóbica para mejorar el proceso de elaboración de productos biofarmacéuticos, tales como identificar impurezas durante la purificación de anticuerpos, controlar las variantes de modificación postraduccional durante la producción o caracterizar la relación fármaco-anticuerpo de los conjugados de anticuerpo y fármaco. Los perfiles de separación de los péptidos o las proteínas se generan y se comparan para identificar o calificar los péptidos o las proteínas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962907465P | 2019-09-27 | 2019-09-27 | |
PCT/US2020/052975 WO2021062341A1 (en) | 2019-09-27 | 2020-09-27 | Hydrophobic interaction chromatography-coupled native mass spectrometry for antibody analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022003691A true MX2022003691A (es) | 2022-04-26 |
Family
ID=72896082
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022003691A MX2022003691A (es) | 2019-09-27 | 2020-09-27 | Espectrometria de masas nativa acoplada a cromatografia de interaccion hidrofobica para analisis de anticuerpos. |
Country Status (12)
Country | Link |
---|---|
US (3) | US11525833B2 (es) |
EP (1) | EP4034883A1 (es) |
JP (1) | JP2022549875A (es) |
KR (1) | KR20220071222A (es) |
CN (1) | CN114787630A (es) |
AU (1) | AU2020353178A1 (es) |
BR (1) | BR112022005317A2 (es) |
CA (1) | CA3155664A1 (es) |
IL (1) | IL291660A (es) |
MX (1) | MX2022003691A (es) |
WO (1) | WO2021062341A1 (es) |
ZA (1) | ZA202203400B (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11202005234XA (en) | 2018-01-29 | 2020-07-29 | Ion Opticks Res Pty Ltd | Capillary fitting |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ516848A (en) * | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
ES2549077T3 (es) * | 2004-04-07 | 2015-10-22 | Genentech, Inc. | Espectrometría de masas de conjugados de anticuerpos |
WO2017151892A2 (en) | 2016-03-02 | 2017-09-08 | Waters Technologies Corporation | Identification and quantification of conjugated peptides in antibody drug conjugates by mass spectrometry |
-
2020
- 2020-09-27 EP EP20792800.3A patent/EP4034883A1/en active Pending
- 2020-09-27 AU AU2020353178A patent/AU2020353178A1/en active Pending
- 2020-09-27 MX MX2022003691A patent/MX2022003691A/es unknown
- 2020-09-27 WO PCT/US2020/052975 patent/WO2021062341A1/en unknown
- 2020-09-27 CA CA3155664A patent/CA3155664A1/en active Pending
- 2020-09-27 CN CN202080067616.4A patent/CN114787630A/zh active Pending
- 2020-09-27 BR BR112022005317A patent/BR112022005317A2/pt unknown
- 2020-09-27 KR KR1020227013304A patent/KR20220071222A/ko unknown
- 2020-09-27 US US17/033,820 patent/US11525833B2/en active Active
- 2020-09-27 JP JP2022519148A patent/JP2022549875A/ja active Pending
-
2022
- 2022-03-23 ZA ZA2022/03400A patent/ZA202203400B/en unknown
- 2022-03-24 IL IL291660A patent/IL291660A/en unknown
- 2022-11-07 US US17/981,839 patent/US11885811B2/en active Active
-
2023
- 2023-12-04 US US18/527,919 patent/US20240110924A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN114787630A (zh) | 2022-07-22 |
JP2022549875A (ja) | 2022-11-29 |
KR20220071222A (ko) | 2022-05-31 |
ZA202203400B (en) | 2024-01-31 |
BR112022005317A2 (pt) | 2022-06-14 |
US11885811B2 (en) | 2024-01-30 |
WO2021062341A1 (en) | 2021-04-01 |
US20210102951A1 (en) | 2021-04-08 |
US11525833B2 (en) | 2022-12-13 |
US20230251266A1 (en) | 2023-08-10 |
AU2020353178A1 (en) | 2022-04-14 |
US20240110924A1 (en) | 2024-04-04 |
IL291660A (en) | 2022-05-01 |
CA3155664A1 (en) | 2021-04-01 |
EP4034883A1 (en) | 2022-08-03 |
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