MX2022003438A - Sistemas y metodos para uso y regeneracion de cromatografia. - Google Patents

Sistemas y metodos para uso y regeneracion de cromatografia.

Info

Publication number
MX2022003438A
MX2022003438A MX2022003438A MX2022003438A MX2022003438A MX 2022003438 A MX2022003438 A MX 2022003438A MX 2022003438 A MX2022003438 A MX 2022003438A MX 2022003438 A MX2022003438 A MX 2022003438A MX 2022003438 A MX2022003438 A MX 2022003438A
Authority
MX
Mexico
Prior art keywords
alkaline solution
hydrophobic interaction
regeneration
systems
methods
Prior art date
Application number
MX2022003438A
Other languages
English (en)
Inventor
John Mattila
James Reilly
Robert Stairs
Samantha Wadsworth
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022003438A publication Critical patent/MX2022003438A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/34—Regenerating or reactivating
    • B01J20/345—Regenerating or reactivating using a particular desorbing compound or mixture
    • B01J20/3475—Regenerating or reactivating using a particular desorbing compound or mixture in the liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/40—Flow patterns using back flushing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/203—Equilibration or regeneration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Aspectos de la presente divulgación se refieren a un método de regeneración de una columna de cromatografía de interacción hidrofóbica a la que se ha aplicado una masa de carga, comprendiendo el método pasar uno o más volúmenes de columna de una solución alcalina a través de medios de interacción hidrofóbica dentro de la columna, donde la solución alcalina presenta un pH de entre aproximadamente 10 y aproximadamente 14, y una conductividad de entre 0,5 mS/cm y aproximadamente 10 mS/cm, donde se elimina el material unido a los medios de interacción hidrofóbica. En algunos casos, la solución alcalina puede incluir hidróxido de sodio a una concentración de entre, por ejemplo, aproximadamente 0,1 mM y 10 mM.
MX2022003438A 2019-09-24 2020-09-23 Sistemas y metodos para uso y regeneracion de cromatografia. MX2022003438A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962905033P 2019-09-24 2019-09-24
US202062958899P 2020-01-09 2020-01-09
PCT/US2020/052243 WO2021061790A1 (en) 2019-09-24 2020-09-23 Systems and methods for chromatography use and regeneration

Publications (1)

Publication Number Publication Date
MX2022003438A true MX2022003438A (es) 2022-04-19

Family

ID=72896066

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022003438A MX2022003438A (es) 2019-09-24 2020-09-23 Sistemas y metodos para uso y regeneracion de cromatografia.

Country Status (12)

Country Link
US (1) US20220323937A1 (es)
EP (1) EP4034870A1 (es)
JP (1) JP2022548394A (es)
KR (1) KR20220066393A (es)
CN (1) CN114667450A (es)
AU (1) AU2020352547A1 (es)
BR (1) BR112022004885A2 (es)
CA (2) CA3216345A1 (es)
CO (1) CO2022003888A2 (es)
IL (1) IL291020A (es)
MX (1) MX2022003438A (es)
WO (1) WO2021061790A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023191827A1 (en) * 2022-03-28 2023-10-05 President And Fellows Of Harvard College High efficiency purification of divergent aav serotypes using aavx affinity chromatography

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Also Published As

Publication number Publication date
CO2022003888A2 (es) 2022-04-19
CN114667450A (zh) 2022-06-24
WO2021061790A1 (en) 2021-04-01
CA3150234C (en) 2023-12-12
US20220323937A1 (en) 2022-10-13
CA3150234A1 (en) 2021-04-01
AU2020352547A1 (en) 2022-03-31
KR20220066393A (ko) 2022-05-24
CA3216345A1 (en) 2021-04-01
IL291020A (en) 2022-05-01
BR112022004885A2 (pt) 2022-06-07
EP4034870A1 (en) 2022-08-03
JP2022548394A (ja) 2022-11-18

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