MX2022001349A - Metodos para purificar anticuerpos. - Google Patents
Metodos para purificar anticuerpos.Info
- Publication number
- MX2022001349A MX2022001349A MX2022001349A MX2022001349A MX2022001349A MX 2022001349 A MX2022001349 A MX 2022001349A MX 2022001349 A MX2022001349 A MX 2022001349A MX 2022001349 A MX2022001349 A MX 2022001349A MX 2022001349 A MX2022001349 A MX 2022001349A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- recombinant antibody
- purifying antibodies
- imidazole
- purifying
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N2030/381—Flow patterns centrifugal chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Abstract
La presente invención se refiere al campo de la producción de moléculas de anticuerpos recombinantes. En particular, se proporcionan métodos para purificar tales moléculas de anticuerpo recombinante en las que se agrega imidazol o un análogo de imidazol durante la elución de la molécula de anticuerpo recombinante a partir de una resina de cromatografía de afinidad, tal como una resina basada en proteína A.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP19189841 | 2019-08-02 | ||
PCT/EP2020/071533 WO2021023619A1 (en) | 2019-08-02 | 2020-07-30 | Methods for purifying antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022001349A true MX2022001349A (es) | 2022-03-25 |
Family
ID=67658409
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022001349A MX2022001349A (es) | 2019-08-02 | 2020-07-30 | Metodos para purificar anticuerpos. |
Country Status (13)
Country | Link |
---|---|
US (1) | US20220280887A1 (es) |
EP (1) | EP4007771A1 (es) |
JP (1) | JP2022543187A (es) |
KR (1) | KR20220039744A (es) |
CN (1) | CN114401984A (es) |
AR (1) | AR119546A1 (es) |
AU (1) | AU2020326087A1 (es) |
BR (1) | BR112021023542A2 (es) |
CA (1) | CA3142610A1 (es) |
IL (1) | IL289959A (es) |
MX (1) | MX2022001349A (es) |
SG (1) | SG11202112846RA (es) |
WO (1) | WO2021023619A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20230092867A1 (en) * | 2020-01-20 | 2023-03-23 | WuXi Biologics Ireland Limited | A novel wash buffer solution for affinity chromatography |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5726293A (en) * | 1992-10-02 | 1998-03-10 | The General Hospital Corporation | Affinity purification methods involving imidazole elution |
US20040063153A1 (en) * | 2002-08-12 | 2004-04-01 | Tomas Jelinek | Method for isolation of protein complexes using affinity binding |
US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
UA112203C2 (uk) | 2011-11-11 | 2016-08-10 | Юсб Фарма С.А. | Злитий білок біоспецифічного антитіла, який зв'язується з ox40 людини та сироватковим альбуміном людини |
CA2856216C (en) | 2011-11-11 | 2021-01-12 | Ucb Pharma S.A. | Albumin binding antibodies and binding fragments thereof |
GB201208370D0 (en) | 2012-05-14 | 2012-06-27 | Ucb Pharma Sa | Antibodies |
JP6236948B2 (ja) * | 2013-07-17 | 2017-11-29 | 東ソー株式会社 | 抗体精製用溶出液および当該溶出液を用いた抗体精製方法 |
GB201411320D0 (en) | 2014-06-25 | 2014-08-06 | Ucb Biopharma Sprl | Antibody construct |
GB201506868D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method for protein purification |
-
2020
- 2020-07-30 JP JP2022501242A patent/JP2022543187A/ja active Pending
- 2020-07-30 US US17/631,897 patent/US20220280887A1/en active Pending
- 2020-07-30 WO PCT/EP2020/071533 patent/WO2021023619A1/en unknown
- 2020-07-30 AU AU2020326087A patent/AU2020326087A1/en active Pending
- 2020-07-30 EP EP20757539.0A patent/EP4007771A1/en active Pending
- 2020-07-30 CA CA3142610A patent/CA3142610A1/en active Pending
- 2020-07-30 CN CN202080044910.3A patent/CN114401984A/zh active Pending
- 2020-07-30 KR KR1020227004877A patent/KR20220039744A/ko unknown
- 2020-07-30 BR BR112021023542A patent/BR112021023542A2/pt unknown
- 2020-07-30 SG SG11202112846RA patent/SG11202112846RA/en unknown
- 2020-07-30 MX MX2022001349A patent/MX2022001349A/es unknown
- 2020-07-31 AR ARP200102156A patent/AR119546A1/es unknown
-
2022
- 2022-01-18 IL IL289959A patent/IL289959A/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2022543187A (ja) | 2022-10-11 |
AR119546A1 (es) | 2021-12-29 |
IL289959A (en) | 2022-03-01 |
BR112021023542A2 (pt) | 2022-03-22 |
EP4007771A1 (en) | 2022-06-08 |
WO2021023619A1 (en) | 2021-02-11 |
CA3142610A1 (en) | 2021-02-11 |
CN114401984A (zh) | 2022-04-26 |
US20220280887A1 (en) | 2022-09-08 |
KR20220039744A (ko) | 2022-03-29 |
SG11202112846RA (en) | 2021-12-30 |
AU2020326087A1 (en) | 2021-12-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX355255B (es) | Variantes de fc y métodos para su producción. | |
MX2012012119A (es) | Produccion de proteinas heteromultimericas. | |
MX350610B (es) | Proceso para la purificacion de anticuerpos monoclonales. | |
UA117575C2 (uk) | Антитіло, яке специфічно зв'язується з il-17a та його застосування | |
EA201990321A1 (ru) | Биспецифические антителоподобные связывающие белки, которые специфически связываются с cd3 и cd123 | |
RU2016109322A (ru) | Разделение биспецифических антител и побочных продуктов процесса получения биспецифических антител с применением гидроксиапатитной хроматографии | |
MX2021012251A (es) | Metodo para purificar un anticuerpo modificado en la region de fragmento cristalizable (fc). | |
TW200716669A (en) | Method for the purification of antibodies | |
MX2017004117A (es) | Moleculas de enlace, especialmente anticuerpos, enlazadas a l1cam(cd171). | |
MX2020000288A (es) | Cromatografia. | |
WO2018194496A3 (ru) | Моноклональное антитело к pd-l1 | |
MX2009008608A (es) | Anticuerpos monoclonales anti-cxcl13. | |
EP2574631A4 (en) | FILLER FOR AFFINITY CHROMATOGRAPHY AND METHOD FOR ISOLATING IMMUNE LOBULIN | |
MX2023006291A (es) | Metodos para purificar proteinas que contienen fc. | |
MX2010004660A (es) | NUEVOS ANTICUERPOS ESPECIFICOS DE LOS PEPTIDOS ß-AMILOIDES Y SUS USOS COMO AGENTES DE DIAGNOSTICO Y DROGAS. | |
MX2022002024A (es) | Moleculas de union anti-cd123 multimericas biespecificas y usos de estas. | |
NZ781138A (en) | Anti-vegf protein compositions and methods for producing the same | |
ATE529433T1 (de) | Verfahren zur reinigung eines il-18-bindenden proteins | |
MX2021014334A (es) | Agentes afines a aav9. | |
MX2022001349A (es) | Metodos para purificar anticuerpos. | |
MX2021009275A (es) | Moleculas de union especificas. | |
WO2018231339A3 (en) | TYROSINE PHOSPHORYLATED ANTI-PD-1 ANTIBODIES AND USES THEREOF | |
KR20220079910A (ko) | 숙주-세포 단백질을 특성화하는 방법 | |
MX2021007028A (es) | Anticuerpo anti-il-17a y uso del mismo. | |
EA202190459A1 (ru) | Способ и хроматографическая система для определения количества и чистоты мультимерного белка |