MX2021015805A - Kit, sistema, y celda de flujo. - Google Patents
Kit, sistema, y celda de flujo.Info
- Publication number
- MX2021015805A MX2021015805A MX2021015805A MX2021015805A MX2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A
- Authority
- MX
- Mexico
- Prior art keywords
- fluid
- solid support
- library preparation
- deoxyribonucleic acid
- kit
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/005—Beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00698—Measurement and control of process parameters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Abstract
Un ejemplo de un kit incluye un fluido de preparación de genoteca, un fluido de muestra y un fluido de enriquecimiento. El fluido de preparación de genoteca incluye glóbulos de preparación de genoteca, donde cada glóbulo de preparación de genoteca incluye un primer soporte sólido, y un transposoma unido al primer soporte sólido. El fluido incluye una secuencia de ácido desoxirribonucleico genómico. El fluido de enriquecimiento incluye glóbulos de captura diana, donde cada glóbulo de captura diana incluye un segundo soporte sólido, y sondas de captura unidas al segundo soporte sólido. Cada una de las sondas de captura incluye una secuencia de ácido desoxirribonucleico monocatenario que es complementaria a una región diana del ácido desoxirribonucleico genómico en el fluido de muestra.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062966351P | 2020-01-27 | 2020-01-27 | |
PCT/US2021/014919 WO2021154648A2 (en) | 2020-01-27 | 2021-01-25 | Kit, system, and flow cell |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2021015805A true MX2021015805A (es) | 2022-04-01 |
Family
ID=76969806
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021015805A MX2021015805A (es) | 2020-01-27 | 2021-01-25 | Kit, sistema, y celda de flujo. |
Country Status (10)
Country | Link |
---|---|
US (1) | US20210230585A1 (es) |
EP (1) | EP3969610A2 (es) |
JP (1) | JP2023511465A (es) |
KR (1) | KR20220131819A (es) |
CN (1) | CN114207128A (es) |
AU (1) | AU2021213088A1 (es) |
BR (1) | BR112021026701A2 (es) |
CA (1) | CA3145540A1 (es) |
IL (1) | IL289120A (es) |
MX (1) | MX2021015805A (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023052629A2 (en) * | 2021-09-30 | 2023-04-06 | Illumina Cambridge Limited | Blocker methods |
WO2023223033A1 (en) * | 2022-05-18 | 2023-11-23 | Oxford Nanopore Technologies Plc | Compositions and methods for nucleic acid extraction and purification |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013117595A2 (en) * | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
US20140005066A1 (en) * | 2012-06-29 | 2014-01-02 | Advanced Liquid Logic Inc. | Multiplexed PCR and Fluorescence Detection on a Droplet Actuator |
US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
CA2942908C (en) * | 2013-03-15 | 2023-08-15 | Lariat Biosciences, Inc. | Microfluidic methods for manipulating dna |
US9944924B2 (en) * | 2014-01-16 | 2018-04-17 | Illumina, Inc. | Polynucleotide modification on solid support |
US11453875B2 (en) * | 2015-05-28 | 2022-09-27 | Illumina Cambridge Limited | Surface-based tagmentation |
JP7164276B2 (ja) * | 2017-02-21 | 2022-11-01 | イルミナ インコーポレイテッド | リンカーを用いた固定化トランスポソームを使用するタグメンテーション |
ES2933979T3 (es) * | 2018-05-02 | 2023-02-15 | Hahn Schickard Ges Fuer Angewandte Forschung E V | Inmunoensayo para un sistema automatizado |
PE20220773A1 (es) * | 2018-06-29 | 2022-05-16 | Illumina Inc | Cubetas de lectura |
-
2021
- 2021-01-25 CA CA3145540A patent/CA3145540A1/en active Pending
- 2021-01-25 JP JP2021577073A patent/JP2023511465A/ja active Pending
- 2021-01-25 AU AU2021213088A patent/AU2021213088A1/en active Pending
- 2021-01-25 CN CN202180004114.1A patent/CN114207128A/zh active Pending
- 2021-01-25 BR BR112021026701A patent/BR112021026701A2/pt unknown
- 2021-01-25 KR KR1020217042260A patent/KR20220131819A/ko unknown
- 2021-01-25 EP EP21707071.3A patent/EP3969610A2/en active Pending
- 2021-01-25 MX MX2021015805A patent/MX2021015805A/es unknown
- 2021-01-26 US US17/158,926 patent/US20210230585A1/en active Pending
- 2021-12-19 IL IL289120A patent/IL289120A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2021213088A1 (en) | 2022-01-20 |
IL289120A (en) | 2022-02-01 |
CN114207128A (zh) | 2022-03-18 |
CA3145540A1 (en) | 2021-08-05 |
KR20220131819A (ko) | 2022-09-29 |
JP2023511465A (ja) | 2023-03-20 |
EP3969610A2 (en) | 2022-03-23 |
US20210230585A1 (en) | 2021-07-29 |
BR112021026701A2 (pt) | 2022-08-02 |
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