MX2021015805A - Kit, sistema, y celda de flujo. - Google Patents

Kit, sistema, y celda de flujo.

Info

Publication number
MX2021015805A
MX2021015805A MX2021015805A MX2021015805A MX2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A MX 2021015805 A MX2021015805 A MX 2021015805A
Authority
MX
Mexico
Prior art keywords
fluid
solid support
library preparation
deoxyribonucleic acid
kit
Prior art date
Application number
MX2021015805A
Other languages
English (en)
Inventor
Tarun Kumar Khurana
Yir-Shyuan Wu
Tyler Chen
Pallavi Daggumati
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Priority claimed from PCT/US2021/014919 external-priority patent/WO2021154648A2/en
Publication of MX2021015805A publication Critical patent/MX2021015805A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00497Features relating to the solid phase supports
    • B01J2219/005Beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068Means for controlling the apparatus of the process
    • B01J2219/00698Measurement and control of process parameters
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

Abstract

Un ejemplo de un kit incluye un fluido de preparación de genoteca, un fluido de muestra y un fluido de enriquecimiento. El fluido de preparación de genoteca incluye glóbulos de preparación de genoteca, donde cada glóbulo de preparación de genoteca incluye un primer soporte sólido, y un transposoma unido al primer soporte sólido. El fluido incluye una secuencia de ácido desoxirribonucleico genómico. El fluido de enriquecimiento incluye glóbulos de captura diana, donde cada glóbulo de captura diana incluye un segundo soporte sólido, y sondas de captura unidas al segundo soporte sólido. Cada una de las sondas de captura incluye una secuencia de ácido desoxirribonucleico monocatenario que es complementaria a una región diana del ácido desoxirribonucleico genómico en el fluido de muestra.
MX2021015805A 2020-01-27 2021-01-25 Kit, sistema, y celda de flujo. MX2021015805A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062966351P 2020-01-27 2020-01-27
PCT/US2021/014919 WO2021154648A2 (en) 2020-01-27 2021-01-25 Kit, system, and flow cell

Publications (1)

Publication Number Publication Date
MX2021015805A true MX2021015805A (es) 2022-04-01

Family

ID=76969806

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021015805A MX2021015805A (es) 2020-01-27 2021-01-25 Kit, sistema, y celda de flujo.

Country Status (10)

Country Link
US (1) US20210230585A1 (es)
EP (1) EP3969610A2 (es)
JP (1) JP2023511465A (es)
KR (1) KR20220131819A (es)
CN (1) CN114207128A (es)
AU (1) AU2021213088A1 (es)
BR (1) BR112021026701A2 (es)
CA (1) CA3145540A1 (es)
IL (1) IL289120A (es)
MX (1) MX2021015805A (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023052629A2 (en) * 2021-09-30 2023-04-06 Illumina Cambridge Limited Blocker methods
WO2023223033A1 (en) * 2022-05-18 2023-11-23 Oxford Nanopore Technologies Plc Compositions and methods for nucleic acid extraction and purification

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US20140005066A1 (en) * 2012-06-29 2014-01-02 Advanced Liquid Logic Inc. Multiplexed PCR and Fluorescence Detection on a Droplet Actuator
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CA2942908C (en) * 2013-03-15 2023-08-15 Lariat Biosciences, Inc. Microfluidic methods for manipulating dna
US9944924B2 (en) * 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
US11453875B2 (en) * 2015-05-28 2022-09-27 Illumina Cambridge Limited Surface-based tagmentation
JP7164276B2 (ja) * 2017-02-21 2022-11-01 イルミナ インコーポレイテッド リンカーを用いた固定化トランスポソームを使用するタグメンテーション
ES2933979T3 (es) * 2018-05-02 2023-02-15 Hahn Schickard Ges Fuer Angewandte Forschung E V Inmunoensayo para un sistema automatizado
PE20220773A1 (es) * 2018-06-29 2022-05-16 Illumina Inc Cubetas de lectura

Also Published As

Publication number Publication date
AU2021213088A1 (en) 2022-01-20
IL289120A (en) 2022-02-01
CN114207128A (zh) 2022-03-18
CA3145540A1 (en) 2021-08-05
KR20220131819A (ko) 2022-09-29
JP2023511465A (ja) 2023-03-20
EP3969610A2 (en) 2022-03-23
US20210230585A1 (en) 2021-07-29
BR112021026701A2 (pt) 2022-08-02

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