WO2021154648A3 - Kit, system, and flow cell - Google Patents

Kit, system, and flow cell Download PDF

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Publication number
WO2021154648A3
WO2021154648A3 PCT/US2021/014919 US2021014919W WO2021154648A3 WO 2021154648 A3 WO2021154648 A3 WO 2021154648A3 US 2021014919 W US2021014919 W US 2021014919W WO 2021154648 A3 WO2021154648 A3 WO 2021154648A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
solid support
library preparation
deoxyribonucleic acid
kit
Prior art date
Application number
PCT/US2021/014919
Other languages
French (fr)
Other versions
WO2021154648A2 (en
Inventor
Tyler CHEN
Pallavi DAGGUMATI
Tarun Kumar Khurana
Yir-Shyuan WU
Original Assignee
Illumina, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BR112021026701A priority Critical patent/BR112021026701A2/en
Priority to JP2021577073A priority patent/JP2023511465A/en
Priority to CA3145540A priority patent/CA3145540A1/en
Priority to CN202180004114.1A priority patent/CN114207128A/en
Priority to AU2021213088A priority patent/AU2021213088A1/en
Priority to EP21707071.3A priority patent/EP3969610A2/en
Application filed by Illumina, Inc. filed Critical Illumina, Inc.
Priority to KR1020217042260A priority patent/KR20220131819A/en
Priority to MX2021015805A priority patent/MX2021015805A/en
Priority to US17/158,926 priority patent/US20210230585A1/en
Publication of WO2021154648A2 publication Critical patent/WO2021154648A2/en
Publication of WO2021154648A3 publication Critical patent/WO2021154648A3/en
Priority to IL289120A priority patent/IL289120A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid.
PCT/US2021/014919 2020-01-27 2021-01-25 Kit, system, and flow cell WO2021154648A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
JP2021577073A JP2023511465A (en) 2020-01-27 2021-01-25 Kits, systems and flow cells
CA3145540A CA3145540A1 (en) 2020-01-27 2021-01-25 Kit, system, and flow cell
CN202180004114.1A CN114207128A (en) 2020-01-27 2021-01-25 Kit, system and flow cell
AU2021213088A AU2021213088A1 (en) 2020-01-27 2021-01-25 Kit, system, and flow cell
EP21707071.3A EP3969610A2 (en) 2020-01-27 2021-01-25 Kit, system, and flow cell
BR112021026701A BR112021026701A2 (en) 2020-01-27 2021-01-25 KIT, SYSTEM AND FLOW CELL
KR1020217042260A KR20220131819A (en) 2020-01-27 2021-01-25 Kits, systems and flow cells
MX2021015805A MX2021015805A (en) 2020-01-27 2021-01-25 Kit, system, and flow cell.
US17/158,926 US20210230585A1 (en) 2020-01-27 2021-01-26 Kit, system, and flow cell
IL289120A IL289120A (en) 2020-01-27 2021-12-19 Kit, system, and flow cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062966351P 2020-01-27 2020-01-27
US62/966,351 2020-01-27

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/158,926 Continuation US20210230585A1 (en) 2020-01-27 2021-01-26 Kit, system, and flow cell

Publications (2)

Publication Number Publication Date
WO2021154648A2 WO2021154648A2 (en) 2021-08-05
WO2021154648A3 true WO2021154648A3 (en) 2021-09-10

Family

ID=74672421

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/014919 WO2021154648A2 (en) 2020-01-27 2021-01-25 Kit, system, and flow cell

Country Status (1)

Country Link
WO (1) WO2021154648A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230302422A1 (en) * 2022-03-22 2023-09-28 Illumina, Inc. Chemical planar array
WO2023223033A1 (en) * 2022-05-18 2023-11-23 Oxford Nanopore Technologies Plc Compositions and methods for nucleic acid extraction and purification

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US20140005066A1 (en) * 2012-06-29 2014-01-02 Advanced Liquid Logic Inc. Multiplexed PCR and Fluorescence Detection on a Droplet Actuator
WO2014145555A1 (en) * 2013-03-15 2014-09-18 Lariat Biosciences, Inc. Microfluidic methods for manipulating dna
US20150353926A1 (en) * 2014-01-16 2015-12-10 Illumina Cambridge Limited Polynucleotide modification on solid support
WO2016189331A1 (en) * 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
WO2018156519A1 (en) * 2017-02-21 2018-08-30 Illumina Inc. Tagmentation using immobilized transposomes with linkers
WO2019211218A1 (en) * 2018-05-02 2019-11-07 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Immunoassay for an automated system
WO2020005503A1 (en) * 2018-06-29 2020-01-02 Illumina, Inc. Flow cells

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US20140005066A1 (en) * 2012-06-29 2014-01-02 Advanced Liquid Logic Inc. Multiplexed PCR and Fluorescence Detection on a Droplet Actuator
WO2014145555A1 (en) * 2013-03-15 2014-09-18 Lariat Biosciences, Inc. Microfluidic methods for manipulating dna
US20150353926A1 (en) * 2014-01-16 2015-12-10 Illumina Cambridge Limited Polynucleotide modification on solid support
WO2016189331A1 (en) * 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
WO2018156519A1 (en) * 2017-02-21 2018-08-30 Illumina Inc. Tagmentation using immobilized transposomes with linkers
WO2019211218A1 (en) * 2018-05-02 2019-11-07 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Immunoassay for an automated system
WO2020005503A1 (en) * 2018-06-29 2020-01-02 Illumina, Inc. Flow cells

Also Published As

Publication number Publication date
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