MX2021013017A - Metodos de alto rendimiento para aislar y caracterizar biblioteca de mutantes excretadores de amonio generadas por mutagenesis quimica. - Google Patents

Metodos de alto rendimiento para aislar y caracterizar biblioteca de mutantes excretadores de amonio generadas por mutagenesis quimica.

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Publication number
MX2021013017A
MX2021013017A MX2021013017A MX2021013017A MX2021013017A MX 2021013017 A MX2021013017 A MX 2021013017A MX 2021013017 A MX2021013017 A MX 2021013017A MX 2021013017 A MX2021013017 A MX 2021013017A MX 2021013017 A MX2021013017 A MX 2021013017A
Authority
MX
Mexico
Prior art keywords
methods
nitrogen
ammonium
characterizing
isolating
Prior art date
Application number
MX2021013017A
Other languages
English (en)
Inventor
Alvin Tamsir
Bilge Ozaydin Eskiyenenturk
Min-Hyung Ryu
Original Assignee
Pivot Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of MX2021013017A publication Critical patent/MX2021013017A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26—Klebsiella (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente descripción proporciona métodos de alto rendimiento para mutagenizar, seleccionar y dirigir rápidamente microbios candidatos que son capaces de fijar nitrógeno atmosférico en presencia de nitrógeno exógeno. Los métodos utilizan un biosensor microbiano capaz de detectar la presencia/ausencia de amonio y/o glutamina en una composición y señalizar con un indicador fluorescente. La presente descripción utiliza además ensayos de detección visual rápida capaces de procesar miles de microbios candidatos. Los métodos y el biosensor descritos se pueden usar para identificar bacterias mutantes con capacidades de fijación de nitrógeno mejoradas. También se describen bacterias mutantes con capacidades de fijación de nitrógeno mejoradas, así como métodos para utilizar estas nuevas bacterias para proporcionar nitrógeno fijo a una planta.
MX2021013017A 2019-04-25 2020-04-24 Metodos de alto rendimiento para aislar y caracterizar biblioteca de mutantes excretadores de amonio generadas por mutagenesis quimica. MX2021013017A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962838780P 2019-04-25 2019-04-25
PCT/US2020/029831 WO2020219893A2 (en) 2019-04-25 2020-04-24 High-throughput methods for isolating and characterizing ammonium-excreting mutant libraries generated by chemical mutagenesis

Publications (1)

Publication Number Publication Date
MX2021013017A true MX2021013017A (es) 2021-12-10

Family

ID=72941421

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021013017A MX2021013017A (es) 2019-04-25 2020-04-24 Metodos de alto rendimiento para aislar y caracterizar biblioteca de mutantes excretadores de amonio generadas por mutagenesis quimica.

Country Status (11)

Country Link
US (1) US20220282340A1 (es)
EP (1) EP3959302A4 (es)
KR (1) KR20220004047A (es)
CN (1) CN114008221A (es)
AR (1) AR118792A1 (es)
AU (1) AU2020263508A1 (es)
BR (1) BR112021021357A2 (es)
CA (1) CA3129539A1 (es)
MX (1) MX2021013017A (es)
UY (1) UY38676A (es)
WO (1) WO2020219893A2 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
CA2991776A1 (en) 2015-07-13 2017-01-19 Pivot Bio, Inc. Methods and compositions for improving plant traits
US11479516B2 (en) 2015-10-05 2022-10-25 Massachusetts Institute Of Technology Nitrogen fixation using refactored NIF clusters
CN110799474B (zh) 2017-01-12 2022-07-26 皮沃特生物公司 用于改良植物性状的方法及组合物
CN111587287A (zh) 2017-10-25 2020-08-25 皮沃特生物股份有限公司 用于改良固氮的工程微生物的方法和组合物
CN112739668A (zh) 2018-06-27 2021-04-30 皮沃特生物股份有限公司 包括重构固氮微生物的农业组合物
CN112481169B (zh) * 2020-12-08 2022-07-22 山东土地集团威海有限公司 一种耐盐碱泌铵型自生固氮菌株及其应用
AR124494A1 (es) 2020-12-23 2023-04-05 Pivot Bio Inc Sistemas, métodos y composiciones agrícolas para el tratamiento de semillas
CN113248582A (zh) * 2021-05-11 2021-08-13 西安交通大学 一种固氮微生物的改造与调控方法
CN114518358B (zh) * 2022-02-16 2023-10-27 四川农业大学 农药残留检测用三色传感探针及其制备方法、应用和深度学习的视觉智能监测装置及方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9279139B2 (en) * 2011-06-21 2016-03-08 University Of Guelph Media comprising a glutamine biosensor and methods of use thereof
CA2991776A1 (en) * 2015-07-13 2017-01-19 Pivot Bio, Inc. Methods and compositions for improving plant traits
CN110799474B (zh) * 2017-01-12 2022-07-26 皮沃特生物公司 用于改良植物性状的方法及组合物
KR20200044015A (ko) * 2017-08-09 2020-04-28 피벗 바이오, 인크. 유전자조작 미생물을 개선하기 위한 방법 및 조성물

Also Published As

Publication number Publication date
WO2020219893A2 (en) 2020-10-29
AU2020263508A1 (en) 2021-10-14
KR20220004047A (ko) 2022-01-11
CN114008221A (zh) 2022-02-01
EP3959302A4 (en) 2023-06-21
US20220282340A1 (en) 2022-09-08
EP3959302A2 (en) 2022-03-02
CA3129539A1 (en) 2020-10-29
WO2020219893A3 (en) 2020-12-24
AR118792A1 (es) 2021-11-03
BR112021021357A2 (pt) 2022-02-08
UY38676A (es) 2020-11-30

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